Cytochrome b-c1 complex subunit 2, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P07257 (QCR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 135. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome b-c1 complex subunit 2, mitochondrial

Alternative name(s):
Complex III subunit 2
Core protein II
Ubiquinol-cytochrome-c reductase complex core protein 2

Gene names

Name:	QCR2
Synonyms:	COR2, UCR2
Ordered Locus Names:	YPR191W
ORF Names:	P9677.6

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The complex couples electron transfer from ubiquinol to cytochrome c. QCR2 is required for the assembly of the complex.
Subunit structure	Fungi cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.
Subcellular location	Mitochondrion inner membrane Ref.5.
Miscellaneous	Present with 35700 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.1. Source: SGD mitochondrial electron transport, ubiquinol to cytochrome c Inferred from direct assay Ref.1. Source: SGD proteolysis Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial crista Inferred from direct assay. Source: SGD mitochondrial respiratory chain complex III Inferred from direct assay Ref.8. Source: SGD
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COR1	P07256	5	EBI-19929,EBI-19922

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 16

Mitochondrion

Chain

17 – 368

352

Cytochrome b-c1 complex subunit 2, mitochondrial

PRO_0000026801

Amino acid modifications

Modified residue

141

Phosphoserine Ref.7

Secondary structure

368

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07257 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 51FDE1C5DA4420D0
Show »

FASTA

368

40,478

        10         20         30         40         50         60 
MLSAARLQFA QGSVRRLTVS ARDAPTKIST LAVKVHGGSR YATKDGVAHL LNRFNFQNTN 

        70         80         90        100        110        120 
TRSALKLVRE SELLGGTFKS TLDREYITLK ATFLKDDLPY YVNALADVLY KTAFKPHELT 

       130        140        150        160        170        180 
ESVLPAARYD YAVAEQCPVK SAEDQLYAIT FRKGLGNPLL YDGVERVSLQ DIKDFADKVY 

       190        200        210        220        230        240 
TKENLEVSGE NVVEADLKRF VDESLLSTLP AGKSLVSKSE PKFFLGEENR VRFIGDSVAA 

       250        260        270        280        290        300 
IGIPVNKASL AQYEVLANYL TSALSELSGL ISSAKLDKFT DGGLFTLFVR DQDSAVVSSN 

       310        320        330        340        350        360 
IKKIVADLKK GKDLSPAINY TKLKNAVQNE SVSSPIELNF DAVKDFKLGK FNYVAVGDVS 


NLPYLDEL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subunit II of yeast QH2:cytochrome-c oxidoreductase. Nucleotide sequence of the gene and features of the protein." Oudshoorn P., van Steeg H., Swinkels B.W., Schoppink P., Grivell L.A. Eur. J. Biochem. 163:97-103(1987) [PubMed: 3028797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[8]	"Structure at 2.3 A resolution of the cytochrome bc1 complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment." Hunte C., Koepke J., Lange C., Rossmanith T., Michel H. Structure 8:669-684(2000) [PubMed: 10873857] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[9]	"Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c." Lange C., Hunte C. Proc. Natl. Acad. Sci. U.S.A. 99:2800-2805(2002) [PubMed: 11880631] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.97 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X05120 Genomic DNA. Translation: CAA28768.1.
U25841 Genomic DNA. Translation: AAB64620.1.
AY558068 Genomic DNA. Translation: AAS56394.1.
BK006949 Genomic DNA. Translation: DAA11607.1.

PIR

A27535.

RefSeq

NP_015517.1. NM_001184288.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EZV	X-ray	2.30	B	17-368	[»]
1KB9	X-ray	2.30	B	17-368	[»]
1KYO	X-ray	2.97	B/M	17-368	[»]
1P84	X-ray	2.50	B	17-368	[»]
2IBZ	X-ray	2.30	B	17-368	[»]
3CX5	X-ray	1.90	B/M	17-368	[»]
3CXH	X-ray	2.50	B/M	17-368	[»]

ProteinModelPortal

P07257.

SMR

P07257. Positions 17-368.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3820N.

IntAct

P07257. 23 interactions.

MINT

MINT-493086.

STRING

P07257.

Protein family/group databases

MEROPS

M16.974.

Proteomic databases

PeptideAtlas

P07257.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPR191W; YPR191W; YPR191W.

GeneID

856321.

KEGG

sce:YPR191W.

NMPDR

fig|4932.3.peg.6666.

Organism-specific databases

CYGD

YPR191w.

SGD

S000006395. QCR2.

Phylogenomic databases

eggNOG

fuNOG04216.

GeneTree

EFGT00050000004568.

HOGENOM

HBG737788.

OMA

KFNYVAV.

OrthoDB

EOG4Q2HPZ.

Gene expression databases

ArrayExpress

P07257.

Genevestigator

P07257.

GermOnline

YPR191W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR011249. Metalloenz_metal-bd.
IPR011237. Pept_M16_core.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]

Gene3D

G3DSA:3.30.830.10. Pept_M16_core. 2 hits.

K00415.

Pfam

PF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]

SUPFAM

SSF63411. Metalloenz_metal-bd. 2 hits.

PROSITE

PS00143. INSULINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

981715.

Entry information

Entry name

QCR2_YEAST

Accession

Primary (citable) accession number: P07257
Secondary accession number(s): D6W4J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	December 14, 2011
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents