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P07254

- CHIA_SERMA

UniProt

P07254 - CHIA_SERMA

Protein

Chitinase A

Gene

chiA

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei315 – 3151Proton donorCurated

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chitin catabolic process Source: UniProtKB-KW
    2. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17691.
    BRENDAi3.2.1.14. 5690.
    SABIO-RKP07254.

    Protein family/group databases

    CAZyiGH18. Glycoside Hydrolase Family 18.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chitinase A (EC:3.2.1.14)
    Gene namesi
    Name:chiA
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 563540Chitinase APRO_0000011908Add
    BLAST

    Interactioni

    Structurei

    Secondary structure

    1
    563
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi37 – 437
    Helixi50 – 534
    Beta strandi54 – 563
    Beta strandi58 – 6710
    Beta strandi69 – 713
    Beta strandi75 – 817
    Beta strandi84 – 907
    Beta strandi93 – 10210
    Beta strandi106 – 11611
    Beta strandi119 – 1224
    Beta strandi126 – 1316
    Beta strandi156 – 16510
    Helixi166 – 1694
    Beta strandi170 – 1723
    Helixi176 – 1783
    Helixi181 – 1833
    Beta strandi185 – 1928
    Turni198 – 2003
    Helixi202 – 2065
    Helixi210 – 2178
    Turni218 – 2203
    Helixi231 – 2355
    Helixi251 – 26212
    Beta strandi267 – 2737
    Turni275 – 2773
    Helixi279 – 2835
    Helixi287 – 30317
    Beta strandi309 – 3135
    Helixi331 – 35323
    Beta strandi358 – 3647
    Helixi367 – 3704
    Helixi375 – 3784
    Helixi379 – 3813
    Beta strandi383 – 3886
    Beta strandi392 – 3943
    Beta strandi398 – 4003
    Helixi420 – 43011
    Helixi434 – 4363
    Beta strandi437 – 45014
    Helixi459 – 4613
    Beta strandi465 – 4673
    Beta strandi471 – 4733
    Beta strandi476 – 4783
    Helixi479 – 4857
    Beta strandi486 – 4883
    Beta strandi492 – 4965
    Turni497 – 5004
    Beta strandi501 – 5066
    Turni507 – 5104
    Beta strandi511 – 5144
    Helixi518 – 53114
    Beta strandi535 – 5395
    Helixi541 – 5433
    Helixi547 – 5559

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CTNX-ray2.30A24-563[»]
    1EHNX-ray1.90A24-563[»]
    1EIBX-ray1.80A24-563[»]
    1FFQX-ray1.90A24-563[»]
    1FFRX-ray1.80A24-563[»]
    1K9TX-ray1.80A24-563[»]
    1NH6X-ray2.05A24-563[»]
    1RD6X-ray2.60A1-563[»]
    1X6LX-ray1.90A1-563[»]
    1X6NX-ray2.00A1-563[»]
    2WK2X-ray2.05A24-563[»]
    2WLYX-ray2.40A24-563[»]
    2WLZX-ray1.82A24-563[»]
    2WM0X-ray1.90A24-563[»]
    ProteinModelPortaliP07254.
    SMRiP07254. Positions 24-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07254.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 563414CatalyticAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR013540. ChitinaseA_N.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR022409. PKD/Chitinase_dom.
    [Graphical view]
    PfamiPF08329. ChitinaseA_N. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view]
    SMARTiSM00636. Glyco_18. 1 hit.
    SM00089. PKD. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS01095. CHITINASE_18. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07254-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKFNKPLLA LLIGSTLCSA AQAAAPGKPT IAWGNTKFAI VEVDQAATAY    50
    NNLVKVKNAA DVSVSWNLWN GDAGTTAKIL LNGKEAWSGP STGSSGTANF 100
    KVNKGGRYQM QVALCNADGC TASDATEIVV ADTDGSHLAP LKEPLLEKNK 150
    PYKQNSGKVV GSYFVEWGVY GRNFTVDKIP AQNLTHLLYG FIPICGGNGI 200
    NDSLKEIEGS FQALQRSCQG REDFKVSIHD PFAALQKAQK GVTAWDDPYK 250
    GNFGQLMALK QAHPDLKILP SIGGWTLSDP FFFMGDKVKR DRFVGSVKEF 300
    LQTWKFFDGV DIDWEFPGGK GANPNLGSPQ DGETYVLLMK ELRAMLDQLS 350
    AETGRKYELT SAISAGKDKI DKVAYNVAQN SMDHIFLMSY DFYGPFDLKN 400
    LGHQTALNAP AWKPDTAYTT VNGVNALLAQ GVKPGKVVVG TAMYGRGWTG 450
    VNGYQNNIPF TGTATGPVKG TWKNGIVDYR QIAGQFMSGE WQYTYDATAE 500
    APYVFKPSTG DLITFDDARS VQAKGKYVLD KQLGGLFSWE IDADNGDILN 550
    SMNASLGNSA GVQ 563
    Length:563
    Mass (Da):60,979
    Last modified:February 1, 1995 - v3
    Checksum:i0696FEF6AF83AA35
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521N → S in CAA85291. (PubMed:7851747)Curated
    Sequence conflicti73 – 731A → T in CAA85291. (PubMed:7851747)Curated
    Sequence conflicti76 – 772TA → GP in CAA27292. (PubMed:16453672)Curated
    Sequence conflicti79 – 791I → V in CAA85291. (PubMed:7851747)Curated
    Sequence conflicti121 – 1211T → S in CAA85291. (PubMed:7851747)Curated
    Sequence conflicti139 – 1391A → P in CAA27292. (PubMed:16453672)Curated
    Sequence conflicti226 – 2261V → I in CAA27292. (PubMed:16453672)Curated
    Sequence conflicti395 – 3951P → A1 PublicationCurated
    Sequence conflicti395 – 3951P → A(PubMed:16453672)Curated
    Sequence conflicti395 – 3951P → A(PubMed:7851747)Curated
    Sequence conflicti410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG1 PublicationCuratedAdd
    BLAST
    Sequence conflicti410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG(PubMed:16453672)CuratedAdd
    BLAST
    Sequence conflicti437 – 4371V → I(PubMed:16453672)Curated
    Sequence conflicti437 – 4371V → I(PubMed:7851747)Curated
    Sequence conflicti464 – 4674ATGP → HRA in CAA27292. (PubMed:16453672)Curated
    Sequence conflicti473 – 4731K → E(PubMed:16453672)Curated
    Sequence conflicti473 – 4731K → E(PubMed:7851747)Curated
    Sequence conflicti484 – 4841G → S in CAA27292. (PubMed:16453672)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01455 Genomic DNA. Translation: AAA26551.1.
    X03657 Genomic DNA. Translation: CAA27292.1.
    Z36294 Genomic DNA. Translation: CAA85291.1.
    PIRiA25090.
    S60651.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01455 Genomic DNA. Translation: AAA26551.1 .
    X03657 Genomic DNA. Translation: CAA27292.1 .
    Z36294 Genomic DNA. Translation: CAA85291.1 .
    PIRi A25090.
    S60651.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CTN X-ray 2.30 A 24-563 [» ]
    1EHN X-ray 1.90 A 24-563 [» ]
    1EIB X-ray 1.80 A 24-563 [» ]
    1FFQ X-ray 1.90 A 24-563 [» ]
    1FFR X-ray 1.80 A 24-563 [» ]
    1K9T X-ray 1.80 A 24-563 [» ]
    1NH6 X-ray 2.05 A 24-563 [» ]
    1RD6 X-ray 2.60 A 1-563 [» ]
    1X6L X-ray 1.90 A 1-563 [» ]
    1X6N X-ray 2.00 A 1-563 [» ]
    2WK2 X-ray 2.05 A 24-563 [» ]
    2WLY X-ray 2.40 A 24-563 [» ]
    2WLZ X-ray 1.82 A 24-563 [» ]
    2WM0 X-ray 1.90 A 24-563 [» ]
    ProteinModelPortali P07254.
    SMRi P07254. Positions 24-563.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P07254.
    ChEMBLi CHEMBL5423.

    Protein family/group databases

    CAZyi GH18. Glycoside Hydrolase Family 18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17691.
    BRENDAi 3.2.1.14. 5690.
    SABIO-RK P07254.

    Miscellaneous databases

    EvolutionaryTracei P07254.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    3.10.50.10. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR011583. Chitinase_II.
    IPR029070. Chitinase_insertion.
    IPR013540. ChitinaseA_N.
    IPR001223. Glyco_hydro18cat.
    IPR001579. Glyco_hydro_18_chit_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR022409. PKD/Chitinase_dom.
    [Graphical view ]
    Pfami PF08329. ChitinaseA_N. 1 hit.
    PF00704. Glyco_hydro_18. 1 hit.
    [Graphical view ]
    SMARTi SM00636. Glyco_18. 1 hit.
    SM00089. PKD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF54556. SSF54556. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS01095. CHITINASE_18. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Koo J.C., Lim C.O., Choi Y.J., Kim C.Y., Bahk J.D., Lee S.Y., Cho M.J.
      Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens."
      Jones J.D.G., Grady K.L., Suslow T.V., Bedbrook J.R.
      EMBO J. 5:467-473(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 990 / QMB1466.
    3. "Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product."
      Brurberg M.B., Eijsink V.G.H., Nes I.F.
      FEMS Microbiol. Lett. 124:399-404(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-31.
      Strain: BJL200.
    4. "Crystal structure of a bacterial chitinase at 2.3-A resolution."
      Perrakis A., Tews I., Dauter Z., Oppenheim A.B., Chet I., Wilson K.S., Vorgias C.E.
      Structure 2:1169-1180(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SEQUENCE REVISION.

    Entry informationi

    Entry nameiCHIA_SERMA
    AccessioniPrimary (citable) accession number: P07254
    Secondary accession number(s): Q54275
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3