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P07254 (CHIA_SERMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chitinase A
EC=3.2.1.14
Gene names
Name:chiA
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sequence similarities

Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.3
Chain24 – 563540Chitinase A
PRO_0000011908

Regions

Region150 – 563414Catalytic

Sites

Active site3151Proton donor Probable

Experimental info

Sequence conflict521N → S in CAA85291. Ref.3
Sequence conflict731A → T in CAA85291. Ref.3
Sequence conflict76 – 772TA → GP in CAA27292. Ref.2
Sequence conflict791I → V in CAA85291. Ref.3
Sequence conflict1211T → S in CAA85291. Ref.3
Sequence conflict1391A → P in CAA27292. Ref.2
Sequence conflict2261V → I in CAA27292. Ref.2
Sequence conflict3951P → A Ref.1
Sequence conflict3951P → A Ref.2
Sequence conflict3951P → A Ref.3
Sequence conflict410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG Ref.1
Sequence conflict410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG Ref.2
Sequence conflict4371V → I Ref.2
Sequence conflict4371V → I Ref.3
Sequence conflict464 – 4674ATGP → HRA in CAA27292. Ref.2
Sequence conflict4731K → E Ref.2
Sequence conflict4731K → E Ref.3
Sequence conflict4841G → S in CAA27292. Ref.2

Secondary structure

1 ................................................................................................. 563
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07254 [UniParc].

Last modified February 1, 1995. Version 3.
Checksum: 0696FEF6AF83AA35

FASTA56360,979
        10         20         30         40         50         60 
MRKFNKPLLA LLIGSTLCSA AQAAAPGKPT IAWGNTKFAI VEVDQAATAY NNLVKVKNAA 

        70         80         90        100        110        120 
DVSVSWNLWN GDAGTTAKIL LNGKEAWSGP STGSSGTANF KVNKGGRYQM QVALCNADGC 

       130        140        150        160        170        180 
TASDATEIVV ADTDGSHLAP LKEPLLEKNK PYKQNSGKVV GSYFVEWGVY GRNFTVDKIP 

       190        200        210        220        230        240 
AQNLTHLLYG FIPICGGNGI NDSLKEIEGS FQALQRSCQG REDFKVSIHD PFAALQKAQK 

       250        260        270        280        290        300 
GVTAWDDPYK GNFGQLMALK QAHPDLKILP SIGGWTLSDP FFFMGDKVKR DRFVGSVKEF 

       310        320        330        340        350        360 
LQTWKFFDGV DIDWEFPGGK GANPNLGSPQ DGETYVLLMK ELRAMLDQLS AETGRKYELT 

       370        380        390        400        410        420 
SAISAGKDKI DKVAYNVAQN SMDHIFLMSY DFYGPFDLKN LGHQTALNAP AWKPDTAYTT 

       430        440        450        460        470        480 
VNGVNALLAQ GVKPGKVVVG TAMYGRGWTG VNGYQNNIPF TGTATGPVKG TWKNGIVDYR 

       490        500        510        520        530        540 
QIAGQFMSGE WQYTYDATAE APYVFKPSTG DLITFDDARS VQAKGKYVLD KQLGGLFSWE 

       550        560 
IDADNGDILN SMNASLGNSA GVQ

« Hide

References

[1]Koo J.C., Lim C.O., Choi Y.J., Kim C.Y., Bahk J.D., Lee S.Y., Cho M.J.
Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens."
Jones J.D.G., Grady K.L., Suslow T.V., Bedbrook J.R.
EMBO J. 5:467-473(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 990 / QMB1466.
[3]"Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product."
Brurberg M.B., Eijsink V.G.H., Nes I.F.
FEMS Microbiol. Lett. 124:399-404(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-31.
Strain: BJL200.
[4]"Crystal structure of a bacterial chitinase at 2.3-A resolution."
Perrakis A., Tews I., Dauter Z., Oppenheim A.B., Chet I., Wilson K.S., Vorgias C.E.
Structure 2:1169-1180(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SEQUENCE REVISION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L01455 Genomic DNA. Translation: AAA26551.1.
X03657 Genomic DNA. Translation: CAA27292.1.
Z36294 Genomic DNA. Translation: CAA85291.1.
PIRA25090.
S60651.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTNX-ray2.30A24-563[»]
1EHNX-ray1.90A24-563[»]
1EIBX-ray1.80A24-563[»]
1FFQX-ray1.90A24-563[»]
1FFRX-ray1.80A24-563[»]
1K9TX-ray1.80A24-563[»]
1NH6X-ray2.05A24-563[»]
1RD6X-ray2.60A1-563[»]
1X6LX-ray1.90A1-563[»]
1X6NX-ray2.00A1-563[»]
2WK2X-ray2.05A24-563[»]
2WLZX-ray1.82A24-563[»]
2WM0X-ray1.90A24-563[»]
ProteinModelPortalP07254.
SMRP07254. Positions 24-563.
ModBaseSearch...

Protein family/group databases

CAZyGH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17691.
BRENDA3.2.1.14. 5690.
SABIO-RKP07254.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.20.20.80. 2 hits.
InterProIPR011583. Chitinase_II.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
[Graphical view]
PfamPF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTSM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view]
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
SSF81296. Ig_E-set. 1 hit.
PROSITEPS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP07254.
ChEMBLCHEMBL5423.
EvolutionaryTraceP07254.

Entry information

Entry nameCHIA_SERMA
AccessionPrimary (citable) accession number: P07254
Secondary accession number(s): Q54275
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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