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P07254

- CHIA_SERMA

UniProt

P07254 - CHIA_SERMA

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Protein

Chitinase A

Gene
chiA
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei315 – 3151Proton donor Inferred

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. chitin catabolic process Source: UniProtKB-KW
  2. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17691.
BRENDAi3.2.1.14. 5690.
SABIO-RKP07254.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase A (EC:3.2.1.14)
Gene namesi
Name:chiA
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 563540Chitinase APRO_0000011908Add
BLAST

Interactioni

Structurei

Secondary structure

1
563
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 437
Helixi50 – 534
Beta strandi54 – 563
Beta strandi58 – 6710
Beta strandi69 – 713
Beta strandi75 – 817
Beta strandi84 – 907
Beta strandi93 – 10210
Beta strandi106 – 11611
Beta strandi119 – 1224
Beta strandi126 – 1316
Beta strandi156 – 16510
Helixi166 – 1694
Beta strandi170 – 1723
Helixi176 – 1783
Helixi181 – 1833
Beta strandi185 – 1928
Turni198 – 2003
Helixi202 – 2065
Helixi210 – 2178
Turni218 – 2203
Helixi231 – 2355
Helixi251 – 26212
Beta strandi267 – 2737
Turni275 – 2773
Helixi279 – 2835
Helixi287 – 30317
Beta strandi309 – 3135
Helixi331 – 35323
Beta strandi358 – 3647
Helixi367 – 3704
Helixi375 – 3784
Helixi379 – 3813
Beta strandi383 – 3886
Beta strandi392 – 3943
Beta strandi398 – 4003
Helixi420 – 43011
Helixi434 – 4363
Beta strandi437 – 45014
Helixi459 – 4613
Beta strandi465 – 4673
Beta strandi471 – 4733
Beta strandi476 – 4783
Helixi479 – 4857
Beta strandi486 – 4883
Beta strandi492 – 4965
Turni497 – 5004
Beta strandi501 – 5066
Turni507 – 5104
Beta strandi511 – 5144
Helixi518 – 53114
Beta strandi535 – 5395
Helixi541 – 5433
Helixi547 – 5559

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CTNX-ray2.30A24-563[»]
1EHNX-ray1.90A24-563[»]
1EIBX-ray1.80A24-563[»]
1FFQX-ray1.90A24-563[»]
1FFRX-ray1.80A24-563[»]
1K9TX-ray1.80A24-563[»]
1NH6X-ray2.05A24-563[»]
1RD6X-ray2.60A1-563[»]
1X6LX-ray1.90A1-563[»]
1X6NX-ray2.00A1-563[»]
2WK2X-ray2.05A24-563[»]
2WLYX-ray2.40A24-563[»]
2WLZX-ray1.82A24-563[»]
2WM0X-ray1.90A24-563[»]
ProteinModelPortaliP07254.
SMRiP07254. Positions 24-563.

Miscellaneous databases

EvolutionaryTraceiP07254.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 563414CatalyticAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
[Graphical view]
PfamiPF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07254-1 [UniParc]FASTAAdd to Basket

« Hide

MRKFNKPLLA LLIGSTLCSA AQAAAPGKPT IAWGNTKFAI VEVDQAATAY    50
NNLVKVKNAA DVSVSWNLWN GDAGTTAKIL LNGKEAWSGP STGSSGTANF 100
KVNKGGRYQM QVALCNADGC TASDATEIVV ADTDGSHLAP LKEPLLEKNK 150
PYKQNSGKVV GSYFVEWGVY GRNFTVDKIP AQNLTHLLYG FIPICGGNGI 200
NDSLKEIEGS FQALQRSCQG REDFKVSIHD PFAALQKAQK GVTAWDDPYK 250
GNFGQLMALK QAHPDLKILP SIGGWTLSDP FFFMGDKVKR DRFVGSVKEF 300
LQTWKFFDGV DIDWEFPGGK GANPNLGSPQ DGETYVLLMK ELRAMLDQLS 350
AETGRKYELT SAISAGKDKI DKVAYNVAQN SMDHIFLMSY DFYGPFDLKN 400
LGHQTALNAP AWKPDTAYTT VNGVNALLAQ GVKPGKVVVG TAMYGRGWTG 450
VNGYQNNIPF TGTATGPVKG TWKNGIVDYR QIAGQFMSGE WQYTYDATAE 500
APYVFKPSTG DLITFDDARS VQAKGKYVLD KQLGGLFSWE IDADNGDILN 550
SMNASLGNSA GVQ 563
Length:563
Mass (Da):60,979
Last modified:February 1, 1995 - v3
Checksum:i0696FEF6AF83AA35
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521N → S in CAA85291. 1 Publication
Sequence conflicti73 – 731A → T in CAA85291. 1 Publication
Sequence conflicti76 – 772TA → GP in CAA27292. 1 Publication
Sequence conflicti79 – 791I → V in CAA85291. 1 Publication
Sequence conflicti121 – 1211T → S in CAA85291. 1 Publication
Sequence conflicti139 – 1391A → P in CAA27292. 1 Publication
Sequence conflicti226 – 2261V → I in CAA27292. 1 Publication
Sequence conflicti395 – 3951P → A1 Publication
Sequence conflicti395 – 3951P → A1 Publication
Sequence conflicti395 – 3951P → A1 Publication
Sequence conflicti410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG1 PublicationAdd
BLAST
Sequence conflicti410 – 42920PAWKP…NALLA → RPGSRHRLHHGERRQCAAG1 PublicationAdd
BLAST
Sequence conflicti437 – 4371V → I1 Publication
Sequence conflicti437 – 4371V → I1 Publication
Sequence conflicti464 – 4674ATGP → HRA in CAA27292. 1 Publication
Sequence conflicti473 – 4731K → E1 Publication
Sequence conflicti473 – 4731K → E1 Publication
Sequence conflicti484 – 4841G → S in CAA27292. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01455 Genomic DNA. Translation: AAA26551.1.
X03657 Genomic DNA. Translation: CAA27292.1.
Z36294 Genomic DNA. Translation: CAA85291.1.
PIRiA25090.
S60651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01455 Genomic DNA. Translation: AAA26551.1 .
X03657 Genomic DNA. Translation: CAA27292.1 .
Z36294 Genomic DNA. Translation: CAA85291.1 .
PIRi A25090.
S60651.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CTN X-ray 2.30 A 24-563 [» ]
1EHN X-ray 1.90 A 24-563 [» ]
1EIB X-ray 1.80 A 24-563 [» ]
1FFQ X-ray 1.90 A 24-563 [» ]
1FFR X-ray 1.80 A 24-563 [» ]
1K9T X-ray 1.80 A 24-563 [» ]
1NH6 X-ray 2.05 A 24-563 [» ]
1RD6 X-ray 2.60 A 1-563 [» ]
1X6L X-ray 1.90 A 1-563 [» ]
1X6N X-ray 2.00 A 1-563 [» ]
2WK2 X-ray 2.05 A 24-563 [» ]
2WLY X-ray 2.40 A 24-563 [» ]
2WLZ X-ray 1.82 A 24-563 [» ]
2WM0 X-ray 1.90 A 24-563 [» ]
ProteinModelPortali P07254.
SMRi P07254. Positions 24-563.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P07254.
ChEMBLi CHEMBL5423.

Protein family/group databases

CAZyi GH18. Glycoside Hydrolase Family 18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-17691.
BRENDAi 3.2.1.14. 5690.
SABIO-RK P07254.

Miscellaneous databases

EvolutionaryTracei P07254.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR013540. ChitinaseA_N.
IPR001223. Glyco_hydro18cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR022409. PKD/Chitinase_dom.
[Graphical view ]
Pfami PF08329. ChitinaseA_N. 1 hit.
PF00704. Glyco_hydro_18. 1 hit.
[Graphical view ]
SMARTi SM00636. Glyco_18. 1 hit.
SM00089. PKD. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS01095. CHITINASE_18. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Koo J.C., Lim C.O., Choi Y.J., Kim C.Y., Bahk J.D., Lee S.Y., Cho M.J.
    Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens."
    Jones J.D.G., Grady K.L., Suslow T.V., Bedbrook J.R.
    EMBO J. 5:467-473(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 990 / QMB1466.
  3. "Characterization of a chitinase gene (chiA) from Serratia marcescens BJL200 and one-step purification of the gene product."
    Brurberg M.B., Eijsink V.G.H., Nes I.F.
    FEMS Microbiol. Lett. 124:399-404(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-31.
    Strain: BJL200.
  4. "Crystal structure of a bacterial chitinase at 2.3-A resolution."
    Perrakis A., Tews I., Dauter Z., Oppenheim A.B., Chet I., Wilson K.S., Vorgias C.E.
    Structure 2:1169-1180(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SEQUENCE REVISION.

Entry informationi

Entry nameiCHIA_SERMA
AccessioniPrimary (citable) accession number: P07254
Secondary accession number(s): Q54275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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