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Protein

ATP synthase subunit alpha, mitochondrial

Gene

ATP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei407Required for activityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 213ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • ATP synthesis coupled proton transport Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28984-MONOMER.
BRENDAi3.6.3.14. 984.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit alpha, mitochondrial
Gene namesi
Name:ATP1
Ordered Locus Names:YBL099W
ORF Names:YBL0827
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL099W.
SGDiS000000195. ATP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • mitochondrial intermembrane space Source: Reactome
  • mitochondrial nucleoid Source: SGD
  • mitochondrial proton-transporting ATP synthase, catalytic core Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291G → D in ATP1-2; growth-defect. 1 Publication1
Mutagenesisi383T → I in ATP1-1; growth-defect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionAdd BLAST35
ChainiPRO_000000243336 – 545ATP synthase subunit alpha, mitochondrialAdd BLAST510

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphoserineCombined sources1
Modified residuei178PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07251.
PRIDEiP07251.

PTM databases

iPTMnetiP07251.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.

Protein-protein interaction databases

BioGridi32606. 97 interactors.
DIPiDIP-3025N.
IntActiP07251. 23 interactors.
MINTiMINT-2786223.

Structurei

Secondary structure

1545
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni62 – 64Combined sources3
Beta strandi65 – 72Combined sources8
Beta strandi75 – 80Combined sources6
Beta strandi88 – 92Combined sources5
Beta strandi97 – 103Combined sources7
Beta strandi108 – 114Combined sources7
Helixi116 – 118Combined sources3
Beta strandi124 – 137Combined sources14
Helixi138 – 140Combined sources3
Beta strandi144 – 146Combined sources3
Beta strandi153 – 155Combined sources3
Beta strandi161 – 167Combined sources7
Beta strandi173 – 176Combined sources4
Beta strandi181 – 183Combined sources3
Helixi188 – 193Combined sources6
Beta strandi203 – 207Combined sources5
Beta strandi208 – 211Combined sources4
Helixi212 – 221Combined sources10
Helixi224 – 227Combined sources4
Beta strandi228 – 230Combined sources3
Turni232 – 234Combined sources3
Beta strandi237 – 244Combined sources8
Helixi247 – 258Combined sources12
Turni259 – 261Combined sources3
Helixi263 – 265Combined sources3
Beta strandi266 – 271Combined sources6
Helixi277 – 296Combined sources20
Beta strandi300 – 306Combined sources7
Helixi308 – 321Combined sources14
Helixi328 – 330Combined sources3
Helixi335 – 343Combined sources9
Beta strandi347 – 349Combined sources3
Turni351 – 354Combined sources4
Beta strandi357 – 365Combined sources9
Beta strandi367 – 369Combined sources3
Helixi374 – 382Combined sources9
Beta strandi383 – 389Combined sources7
Helixi391 – 396Combined sources6
Turni404 – 406Combined sources3
Beta strandi408 – 411Combined sources4
Helixi412 – 415Combined sources4
Helixi418 – 437Combined sources20
Helixi438 – 446Combined sources9
Helixi449 – 465Combined sources17
Helixi475 – 487Combined sources13
Turni488 – 492Combined sources5
Helixi495 – 497Combined sources3
Helixi498 – 512Combined sources15
Helixi514 – 523Combined sources10
Helixi528 – 542Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HLDX-ray2.80A/B/C/J/K/L/S/T/U36-545[»]
2WPDX-ray3.43A/B/C36-545[»]
2XOKX-ray3.01A/B/C1-545[»]
3FKSX-ray3.59A/B/C/J/K/L/S/T/U36-545[»]
3OE7X-ray3.19A/B/C/J/K/L/S/T/U36-545[»]
3OEEX-ray2.74A/B/C/J/K/L/S/T/U36-545[»]
3OEHX-ray3.00A/B/C/J/K/L/S/T/U36-545[»]
3OFNX-ray3.20A/B/C/J/K/L/S/T/U36-545[»]
3ZIAX-ray2.50A/B/C/K/L/M36-545[»]
3ZRYX-ray6.50A/B/C36-545[»]
4B2Qelectron microscopy37.00A/C/a/c61-545[»]
B/b60-545[»]
ProteinModelPortaliP07251.
SMRiP07251.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07251.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074846.
HOGENOMiHOG000130111.
InParanoidiP07251.
KOiK02132.
OMAiQVVSIWA.
OrthoDBiEOG092C1T32.

Family and domain databases

CDDicd01132. F1_ATPase_alpha. 1 hit.
Gene3Di2.40.30.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01346. ATP_synth_alpha_bact. 1 hit.
InterProiIPR023366. ATP_synth_asu-like.
IPR000793. ATP_synth_asu_C.
IPR033732. ATP_synth_F1_a.
IPR005294. ATP_synth_F1_asu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR15184:SF3. PTHR15184:SF3. 1 hit.
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039088. F_ATPase_subunit_alpha. 1 hit.
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00962. atpA. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARTAAIRS LSRTLINSTK AARPAAAALA STRRLASTKA QPTEVSSILE
60 70 80 90 100
ERIKGVSDEA NLNETGRVLA VGDGIARVFG LNNIQAEELV EFSSGVKGMA
110 120 130 140 150
LNLEPGQVGI VLFGSDRLVK EGELVKRTGN IVDVPVGPGL LGRVVDALGN
160 170 180 190 200
PIDGKGPIDA AGRSRAQVKA PGILPRRSVH EPVQTGLKAV DALVPIGRGQ
210 220 230 240 250
RELIIGDRQT GKTAVALDTI LNQKRWNNGS DESKKLYCVY VAVGQKRSTV
260 270 280 290 300
AQLVQTLEQH DAMKYSIIVA ATASEAAPLQ YLAPFTAASI GEWFRDNGKH
310 320 330 340 350
ALIVYDDLSK QAVAYRQLSL LLRRPPGREA YPGDVFYLHS RLLERAAKLS
360 370 380 390 400
EKEGSGSLTA LPVIETQGGD VSAYIPTNVI SITDGQIFLE AELFYKGIRP
410 420 430 440 450
AINVGLSVSR VGSAAQVKAL KQVAGSLKLF LAQYREVAAF AQFGSDLDAS
460 470 480 490 500
TKQTLVRGER LTQLLKQNQY SPLATEEQVP LIYAGVNGHL DGIELSRIGE
510 520 530 540
FESSFLSYLK SNHNELLTEI REKGELSKEL LASLKSATES FVATF
Length:545
Mass (Da):58,608
Last modified:July 27, 2011 - v5
Checksum:iE8041D289B924696
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti311 – 313QAV → ASL in AAA66888 (PubMed:2876995).Curated3
Sequence conflicti321L → M in AAA66888 (PubMed:2876995).Curated1
Sequence conflicti340S → P in CAA56001 (PubMed:7502586).Curated1
Sequence conflicti340S → P in CAA84924 (PubMed:7813418).Curated1
Sequence conflicti385G → A in AAA66888 (PubMed:2876995).Curated1
Sequence conflicti459E → Q in AAA66888 (PubMed:2876995).Curated1
Sequence conflicti479 – 483VPLIY → SMII in AAA66888 (PubMed:2876995).Curated5
Sequence conflicti490 – 493LDGI → SGWY in AAA66888 (PubMed:2876995).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02603 Genomic DNA. Translation: AAA66888.1.
D88458 Genomic DNA. Translation: BAA13613.1.
D37948 Genomic DNA. Translation: BAA22508.1.
X79489 Genomic DNA. Translation: CAA56001.1.
Z35861 Genomic DNA. Translation: CAA84924.1.
AY692969 Genomic DNA. Translation: AAT92988.1.
BK006936 Genomic DNA. Translation: DAA07026.2.
PIRiS45401. PWBYA.
RefSeqiNP_009453.2. NM_001178339.2.

Genome annotation databases

EnsemblFungiiYBL099W; YBL099W; YBL099W.
GeneIDi852177.
KEGGisce:YBL099W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02603 Genomic DNA. Translation: AAA66888.1.
D88458 Genomic DNA. Translation: BAA13613.1.
D37948 Genomic DNA. Translation: BAA22508.1.
X79489 Genomic DNA. Translation: CAA56001.1.
Z35861 Genomic DNA. Translation: CAA84924.1.
AY692969 Genomic DNA. Translation: AAT92988.1.
BK006936 Genomic DNA. Translation: DAA07026.2.
PIRiS45401. PWBYA.
RefSeqiNP_009453.2. NM_001178339.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HLDX-ray2.80A/B/C/J/K/L/S/T/U36-545[»]
2WPDX-ray3.43A/B/C36-545[»]
2XOKX-ray3.01A/B/C1-545[»]
3FKSX-ray3.59A/B/C/J/K/L/S/T/U36-545[»]
3OE7X-ray3.19A/B/C/J/K/L/S/T/U36-545[»]
3OEEX-ray2.74A/B/C/J/K/L/S/T/U36-545[»]
3OEHX-ray3.00A/B/C/J/K/L/S/T/U36-545[»]
3OFNX-ray3.20A/B/C/J/K/L/S/T/U36-545[»]
3ZIAX-ray2.50A/B/C/K/L/M36-545[»]
3ZRYX-ray6.50A/B/C36-545[»]
4B2Qelectron microscopy37.00A/C/a/c61-545[»]
B/b60-545[»]
ProteinModelPortaliP07251.
SMRiP07251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32606. 97 interactors.
DIPiDIP-3025N.
IntActiP07251. 23 interactors.
MINTiMINT-2786223.

Protein family/group databases

TCDBi3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiP07251.

Proteomic databases

MaxQBiP07251.
PRIDEiP07251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL099W; YBL099W; YBL099W.
GeneIDi852177.
KEGGisce:YBL099W.

Organism-specific databases

EuPathDBiFungiDB:YBL099W.
SGDiS000000195. ATP1.

Phylogenomic databases

GeneTreeiENSGT00550000074846.
HOGENOMiHOG000130111.
InParanoidiP07251.
KOiK02132.
OMAiQVVSIWA.
OrthoDBiEOG092C1T32.

Enzyme and pathway databases

BioCyciYEAST:G3O-28984-MONOMER.
BRENDAi3.6.3.14. 984.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP07251.
PROiP07251.

Family and domain databases

CDDicd01132. F1_ATPase_alpha. 1 hit.
Gene3Di2.40.30.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01346. ATP_synth_alpha_bact. 1 hit.
InterProiIPR023366. ATP_synth_asu-like.
IPR000793. ATP_synth_asu_C.
IPR033732. ATP_synth_F1_a.
IPR005294. ATP_synth_F1_asu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR15184:SF3. PTHR15184:SF3. 1 hit.
PfamiPF00006. ATP-synt_ab. 1 hit.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039088. F_ATPase_subunit_alpha. 1 hit.
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00962. atpA. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATPA_YEAST
AccessioniPrimary (citable) accession number: P07251
Secondary accession number(s): D6VPQ6, Q92449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 177 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 41500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.