P07247 (KRUP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein krueppel

Gene names

Name:	Kr
ORF Names:	CG3340

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Krueppel is a gap class segmentation protein. It is involved in the segmentation of the embryo and in the differentiation of the Malpighian tubules.
Subcellular location	Nucleus. Note: Chromatin associated. Ref.6
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers.
Sequence caution	The sequence CAA27148.1 differs from that shown. Reason: Frameshift at position 411.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Gap protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Malpighian tubule morphogenesis Traceable author statement. Source: FlyBase axon guidance Inferred from mutant phenotype. Source: FlyBase chromatin silencing Inferred from mutant phenotype. Source: FlyBase compound eye development Inferred from mutant phenotype. Source: FlyBase ganglion mother cell fate determination Traceable author statement. Source: FlyBase muscle organ development Traceable author statement. Source: FlyBase negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: FlyBase neuroblast fate determination Inferred from expression pattern. Source: FlyBase regulation of development, heterochronic Non-traceable author statement. Source: FlyBase trunk segmentation Traceable author statement. Source: FlyBase ventral cord development Non-traceable author statement. Source: FlyBase wing disc development Inferred from genetic interaction. Source: FlyBase zygotic determination of anterior/posterior axis, embryo Non-traceable author statement. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from direct assay. Source: FlyBase sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: FlyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Protein krueppel		PRO_0000046992
Regions
	Zinc finger	222 – 244	23	C2H2-type 1
	Zinc finger	250 – 272	23	C2H2-type 2
	Zinc finger	278 – 300	23	C2H2-type 3
	Zinc finger	306 – 328	23	C2H2-type 4
	Zinc finger	334 – 354	21	C2H2-type 5
Amino acid modifications
	Modified residue	468	1	Phosphoserine Ref.7
	Modified residue	471	1	Phosphoserine Ref.7
	Modified residue	477	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	153 – 154	2	HE → Q in AAL48902. Ref.4
	Sequence conflict	181 – 182	2	HT → LS in CAA27148. Ref.1
	Sequence conflict	256	1	H → D in CAA27148. Ref.1
	Sequence conflict	329	1	N → T in CAA27148. Ref.1
	Sequence conflict	397	1	G → S in CAA27148. Ref.1
	Sequence conflict	429	1	C → Y Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07247 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 67A94177A701D976	FASTA	502	54,715
	10 20 30 40 50 60 MSISMLQDAQ TRTLAAALAG IKQEDVHLDR SMSLSPPMSA NTSATSAAAI YPAMGLQQAA 70 80 90 100 110 120 AASAFGMLSP TQLLAANRQA AAFMAQLPMS TLANTLFPHN PAALFGAWAA QQSLPPQGTH 130 140 150 160 170 180 LHSPPASPHS PLSTPLGSGK HPLNSPNSTP QHHEPAKKAR KLSVKKEFQT EISMSVNDMY 190 200 210 220 230 240 HTSGGPISPP SSGSSPNSTH DGAGGNAGCV GVSKDPSRDK SFTCKICSRS FGYKHVLQNH 250 260 270 280 290 300 ERTHTGEKPF ECPECHKRFT RDHHLKTHMR LHTGEKPYHC SHCDRQFVQV ANLRRHLRVH 310 320 330 340 350 360 TGERPYTCEI CDGKFSDSNQ LKSHMLVHNG EKPFECERCH MKFRRRHHLM NHKCGIQSPP 370 380 390 400 410 420 TPALSPAMSG DYPVAISAIA IEASTNRFAA MCATYGGSNE SVDMEKATPE DDGPLDLSED 430 440 450 460 470 480 GASSVDGHCS NIARRKAQDI RRVFRLPPPQ IPHVPSDMPE QTEPEDLSMH SPRSIGSHEQ 490 500 TDDIDLYDLD DAPASYMGHQ QH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural homology of the product of the Drosophila Krueppel gene with Xenopus transcription factor IIIA." Rosenberg U.B., Schroeder C., Preiss A., Kienlin A., Cote S., Riede I., Jaeckle H. Nature 319:336-339(1986) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Evolutionary conservation pattern of zinc-finger domains of Drosophila segmentation genes." Sommer R.J., Retzlaff M., Goerlich K., Sander K., Tautz D. Proc. Natl. Acad. Sci. U.S.A. 89:10782-10786(1992) [PubMed: 1438276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 240-314.
[6]	"Drosophila Kruppel gene product produced in a baculovirus expression system is a nuclear phosphoprotein that binds to DNA." Ollo R., Maniatis T. Proc. Natl. Acad. Sci. U.S.A. 84:5700-5704(1987) [PubMed: 3112773] [Abstract] Cited for: SUBCELLULAR LOCATION, DNA-BINDING, PHOSPHORYLATION.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468; SER-471 AND SER-477, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03414 Genomic DNA. Translation: CAA27148.1. Frameshift. AE013599 Genomic DNA. Translation: AAF47321.1. AY071280 mRNA. Translation: AAL48902.1.
PIR	TWFF. A24566.
RefSeq	NP_523867.1. NM_079143.2.
UniGene	Dm.2786.
3D structure databases
ProteinModelPortal	P07247.
SMR	P07247. Positions 218-438.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07247. 1 interaction.
MINT	MINT-303217.
STRING	P07247.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0072449; FBpp0072351; FBgn0001325.
GeneID	38012.
KEGG	dme:Dmel_CG3340.
NMPDR	fig|7227.3.peg.7460.
Organism-specific databases
CTD	38012.
FlyBase	FBgn0001325. Kr.
Phylogenomic databases
eggNOG	inNOG05986.
GeneTree	EMGT00070000025668.
InParanoid	P07247.
OMA	FTCKICS.
OrthoDB	EOG4W3R4B.
PhylomeDB	P07247.
Gene expression databases
ArrayExpress	P07247.
Bgee	P07247.
GermOnline	CG3340. Drosophila melanogaster.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 5 hits.
KO	K09231.
Pfam	PF00096. zf-C2H2. 4 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 4 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 5 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	806541.

Entry information

Entry name

KRUP_DROME

Accession

Primary (citable) accession number: P07247
Secondary accession number(s): Q8SYW3, Q9W0V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project