Reviewed, UniProtKB/Swiss-Prot P07246 (ADH3_YEAST)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 3, mitochondrial
    EC=1.1.1.1
Alternative name(s):
    YADH-3
    Alcohol dehydrogenase III

Gene names

Name:	ADH3
Ordered Locus Names:	YMR083W
ORF Names:	YM9582.08

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 11600 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from direct assay. Source: SGD amino acid catabolic process to alcohol via Ehrlich pathway Inferred from genetic interaction. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from direct assay. Source: SGD soluble fraction Inferred from direct assay. Source: SGD
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion
	Chain	28 – 375	348	Alcohol dehydrogenase 3, mitochondrial		PRO_0000000879
Regions
	Nucleotide binding	205 – 211	7	NAD By similarity
	Nucleotide binding	296 – 298	3	NAD By similarity
Sites
	Metal binding	71	1	Zinc 1; catalytic
	Metal binding	94	1	Zinc 1; catalytic
	Metal binding	125	1	Zinc 2
	Metal binding	128	1	Zinc 2
	Metal binding	131	1	Zinc 2
	Metal binding	139	1	Zinc 2
	Metal binding	181	1	Zinc 1; catalytic
	Binding site	229	1	NAD By similarity
	Binding site	234	1	NAD By similarity
	Binding site	368	1	NAD By similarity
Amino acid modifications
	Modified residue	310	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	10	1	R → K Ref.1
	Sequence conflict	10	1	R → K Ref.2
	Sequence conflict	45	1	K → N Ref.1
	Sequence conflict	45	1	K → N Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07246-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: AB849BC4E9D4EB8A	FASTA	375	40,370
	10 20 30 40 50 60 MLRTSTLFTR RVQPSLFSRN ILRLQSTAAI PKTQKGVIFY ENKGKLHYKD IPVPEPKPNE 70 80 90 100 110 120 ILINVKYSGV CHTDLHAWHG DWPLPVKLPL VGGHEGAGVV VKLGSNVKGW KVGDLAGIKW 130 140 150 160 170 180 LNGSCMTCEF CESGHESNCP DADLSGYTHD GSFQQFATAD AIQAAKIQQG TDLAEVAPIL 190 200 210 220 230 240 CAGVTVYKAL KEADLKAGDW VAISGAAGGL GSLAVQYATA MGYRVLGIDA GEEKEKLFKK 250 260 270 280 290 300 LGGEVFIDFT KTKNMVSDIQ EATKGGPHGV INVSVSEAAI SLSTEYVRPC GTVVLVGLPA 310 320 330 340 350 360 NAYVKSEVFS HVVKSINIKG SYVGNRADTR EALDFFSRGL IKSPIKIVGL SELPKVYDLM 370 EKGKILGRYV VDTSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pilgrim D., Ciriacy M. Submitted (OCT-1985) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation and DNA sequence of ADH3, a nuclear gene encoding the mitochondrial isozyme of alcohol dehydrogenase in Saccharomyces cerevisiae." Young E.T., Pilgrim D. Mol. Cell. Biol. 5:3024-3034(1985) [PubMed: 2943982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae." Leskovac V., Trivic S., Pericin D. FEMS Yeast Res. 2:481-494(2002) [PubMed: 12702265] [Abstract] Cited for: REVIEW.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K03292 Genomic DNA. Translation: AAA34409.1. Z49259 Genomic DNA. Translation: CAA89229.1. AY692988 Genomic DNA. Translation: AAT93007.1.
PIR	S54458.
RefSeq	NP_013800.1.
3D structure databases
SMR	P07246. Positions 29-375.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4445N.
IntAct	P07246. 47 interactions.
STRING	P07246.
Proteomic databases
PeptideAtlas	P07246.
Genome annotation databases
Ensembl	YMR083W; YMR083W; YMR083W; Saccharomyces cerevisiae. [Genome view]
GeneID	855107.
KEGG	sce:YMR083W.
NMPDR	fig|4932.3.peg.4847.
Organism-specific databases
CYGD	YMR083w.
SGD	S000004688. ADH3.
Phylogenomic databases
HOGENOM	HBG753318.
OMA	INFAVAR.
OrthoDB	EOG95HTDV.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11727.
BRENDA	1.1.1.1. 250.
Gene expression databases
ArrayExpress	P07246.
Genevestigator	P07246.
GermOnline	YMR083W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978437.

Entry information

Entry name

ADH3_YEAST

Accession

Primary (citable) accession number: P07246

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)