Alcohol dehydrogenase 3, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P07246 (ADH3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 131. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase 3, mitochondrial
EC=1.1.1.1

Alternative name(s):
Alcohol dehydrogenase III
YADH-3

Gene names

Name:	ADH3
Ordered Locus Names:	YMR083W
ORF Names:	YM9582.08

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	An alcohol + NAD⁺ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homotetramer.
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 11600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NADH oxidation Inferred from direct assay PubMed 3546317. Source: SGD amino acid catabolic process to alcohol via Ehrlich pathway Inferred from genetic interaction PubMed 12499363. Source: SGD fermentation Inferred from sequence or structural similarity PubMed 3550419. Source: SGD
Cellular_component	mitochondrial matrix Inferred from direct assay PubMed 3550419. Source: SGD
Molecular_function	alcohol dehydrogenase (NAD) activity Inferred from direct assay PubMed 3550419. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NAM7	P30771	4	EBI-2227,EBI-11831

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 27

Mitochondrion

Chain

28 – 375

348

Alcohol dehydrogenase 3, mitochondrial

PRO_0000000879

Regions

Nucleotide binding

205 – 211

NAD By similarity

Nucleotide binding

296 – 298

NAD By similarity

Sites

Metal binding

Zinc 1; catalytic

Metal binding

Zinc 1; catalytic

Metal binding

125

Zinc 2

Metal binding

128

Zinc 2

Metal binding

131

Zinc 2

Metal binding

139

Zinc 2

Metal binding

181

Zinc 1; catalytic

Binding site

229

NAD By similarity

Binding site

234

NAD By similarity

Binding site

368

NAD By similarity

Amino acid modifications

Modified residue

310

Phosphoserine Ref.8

Experimental info

Sequence conflict

R → K in AAA34409. Ref.1

Sequence conflict

K → N in AAA34409. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P07246 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: AB849BC4E9D4EB8A
Show »

FASTA

375

40,370

        10         20         30         40         50         60 
MLRTSTLFTR RVQPSLFSRN ILRLQSTAAI PKTQKGVIFY ENKGKLHYKD IPVPEPKPNE 

        70         80         90        100        110        120 
ILINVKYSGV CHTDLHAWHG DWPLPVKLPL VGGHEGAGVV VKLGSNVKGW KVGDLAGIKW 

       130        140        150        160        170        180 
LNGSCMTCEF CESGHESNCP DADLSGYTHD GSFQQFATAD AIQAAKIQQG TDLAEVAPIL 

       190        200        210        220        230        240 
CAGVTVYKAL KEADLKAGDW VAISGAAGGL GSLAVQYATA MGYRVLGIDA GEEKEKLFKK 

       250        260        270        280        290        300 
LGGEVFIDFT KTKNMVSDIQ EATKGGPHGV INVSVSEAAI SLSTEYVRPC GTVVLVGLPA 

       310        320        330        340        350        360 
NAYVKSEVFS HVVKSINIKG SYVGNRADTR EALDFFSRGL IKSPIKIVGL SELPKVYDLM 

       370 
EKGKILGRYV VDTSK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pilgrim D., Ciriacy M. Submitted (OCT-1985) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation and DNA sequence of ADH3, a nuclear gene encoding the mitochondrial isozyme of alcohol dehydrogenase in Saccharomyces cerevisiae." Young E.T., Pilgrim D. Mol. Cell. Biol. 5:3024-3034(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The three zinc-containing alcohol dehydrogenases from baker's yeast, Saccharomyces cerevisiae." Leskovac V., Trivic S., Pericin D. FEMS Yeast Res. 2:481-494(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K03292 Genomic DNA. Translation: AAA34409.1. Z49259 Genomic DNA. Translation: CAA89229.1. AY692988 Genomic DNA. Translation: AAT93007.1. BK006946 Genomic DNA. Translation: DAA09980.1.
PIR	S54458.
RefSeq	NP_013800.1. NM_001182582.1.
3D structure databases
ProteinModelPortal	P07246.
SMR	P07246. Positions 29-375.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4445N.
IntAct	P07246. 22 interactions.
MINT	MINT-482092.
STRING	4932.YMR083W.
Proteomic databases
PaxDb	P07246.
PeptideAtlas	P07246.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR083W; YMR083W; YMR083W.
GeneID	855107.
KEGG	sce:YMR083W.
Organism-specific databases
CYGD	YMR083w.
SGD	S000004688. ADH3.
Phylogenomic databases
eggNOG	COG1064.
GeneTree	ENSGT00550000075207.
HOGENOM	HOG000294685.
KO	K13953.
OMA	PCRTGWE.
OrthoDB	EOG4Q5CZM.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11727. YEAST:YMR083W-MONOMER.
Gene expression databases
Genevestigator	P07246.
GermOnline	YMR083W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.180.10. 1 hit.
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978437.

Entry information

Entry name

ADH3_YEAST

Accession

Primary (citable) accession number: P07246
Secondary accession number(s): D6VZQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents