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P07245

- C1TC_YEAST

UniProt

P07245 - C1TC_YEAST

Protein

C-1-tetrahydrofolate synthase, cytoplasmic

Gene

ADE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
    5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei194 – 1941NADPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1713NADPBy similarity
    Nucleotide bindingi384 – 3918ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. formate-tetrahydrofolate ligase activity Source: SGD
    3. methenyltetrahydrofolate cyclohydrolase activity Source: SGD
    4. methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: SGD

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. folic acid-containing compound metabolic process Source: SGD
    3. histidine biosynthetic process Source: UniProtKB-KW
    4. methionine biosynthetic process Source: UniProtKB-KW
    5. purine nucleobase biosynthetic process Source: SGD
    6. purine nucleotide biosynthetic process Source: UniProtKB-KW
    7. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Ligase, Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YGR204W-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C-1-tetrahydrofolate synthase, cytoplasmic
    Short name:
    C1-THF synthase
    Including the following 3 domains:
    Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
    Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
    Formyltetrahydrofolate synthetase (EC:6.3.4.3)
    Gene namesi
    Name:ADE3
    Ordered Locus Names:YGR204W
    ORF Names:G7733
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR204w.
    SGDiS000003436. ADE3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi11 – 111C → S: Almost no effect on activity. 1 Publication
    Mutagenesisi144 – 1441C → S: Cyclohydrolase activity is reduced 20-fold. Dehydrogenase Km is increased 7-fold. 1 Publication
    Mutagenesisi257 – 2571C → S: Cyclohydrolase activity is increased 2-fold. Dehydrogenase Km is increased 2-fold. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 946946C-1-tetrahydrofolate synthase, cytoplasmicPRO_0000199326Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei176 – 1761Phosphoserine2 Publications
    Modified residuei318 – 3181Phosphothreonine1 Publication
    Modified residuei322 – 3221Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP07245.
    PaxDbiP07245.
    PeptideAtlasiP07245.

    Expressioni

    Gene expression databases

    GenevestigatoriP07245.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi33457. 33 interactions.
    DIPiDIP-3867N.
    IntActiP07245. 11 interactions.
    MINTiMINT-574376.
    STRINGi4932.YGR204W.

    Structurei

    3D structure databases

    ProteinModelPortaliP07245.
    SMRiP07245. Positions 2-297, 320-946.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 319319Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd
    BLAST
    Regioni51 – 555Substrate bindingBy similarity
    Regioni98 – 1003Substrate bindingBy similarity
    Regioni277 – 2815Substrate bindingBy similarity
    Regioni320 – 946627Formyltetrahydrofolate synthetaseAdd
    BLAST

    Domaini

    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

    Sequence similaritiesi

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

    Phylogenomic databases

    eggNOGiCOG0190.
    GeneTreeiENSGT00750000117401.
    HOGENOMiHOG000040280.
    KOiK00288.
    OMAiHEYLDEN.
    OrthoDBiEOG7K0ZMJ.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    3.40.50.720. 1 hit.
    HAMAPiMF_01543. FTHFS.
    MF_01576. THF_DHG_CYH.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020867. THF_DH/CycHdrlase_CS.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view]
    PRINTSiPR00085. THFDHDRGNASE.
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    PS00766. THF_DHG_CYH_1. 1 hit.
    PS00767. THF_DHG_CYH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07245-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGQVLDGKA CAQQFRSNIA NEIKSIQGHV PGFAPNLAII QVGNRPDSAT    50
    YVRMKRKAAE EAGIVANFIH LDESATEFEV LRYVDQLNED PHTHGIIVQL 100
    PLPAHLDEDR ITSRVLAEKD VDGFGPTNIG ELNKKNGHPF FLPCTPKGII 150
    ELLHKANVTI EGSRSVVIGR SDIVGSPVAE LLKSLNSTVT ITHSKTRDIA 200
    SYLHDADIVV VAIGQPEFVK GEWFKPRDGT SSDKKTVVID VGTNYVADPS 250
    KKSGFKCVGD VEFNEAIKYV HLITPVPGGV GPMTVAMLMQ NTLIAAKRQM 300
    EESSKPLQIP PLPLKLLTPV PSDIDISRAQ QPKLINQLAQ ELGIYSHELE 350
    LYGHYKAKIS PKVIERLQTR QNGKYILVSG ITPTPLGEGK STTTMGLVQA 400
    LTAHLGKPAI ANVRQPSLGP TLGVKGGAAG GGYSQVIPMD EFNLHLTGDI 450
    HAIGAANNLL AAAIDTRMFH ETTQKNDATF YNRLVPRKNG KRKFTPSMQR 500
    RLNRLGIQKT NPDDLTPEEI NKFARLNIDP DTITIKRVVD INDRMLRQIT 550
    IGQAPTEKNH TRVTGFDITV ASELMAILAL SKDLRDMKER IGRVVVAADV 600
    NRSPVTVEDV GCTGALTALL RDAIKPNLMQ TLEGTPVLVH AGPFANISIG 650
    ASSVIADRVA LKLVGTEPEA KTEAGYVVTE AGFDFTMGGE RFFNIKCRSS 700
    GLTPNAVVLV ATVRALKSHG GAPDVKPGQP LPSAYTEENI EFVEKGAANM 750
    CKQIANIKQF GVPVVVAINK FETDTEGEIA AIRKAALEAG AFEAVTSNHW 800
    AEGGKGAIDL AKAVIEASNQ PVDFHFLYDV NSSVEDKLTT IVQKMYGGAA 850
    IDILPEAQRK IDMYKEQGFG NLPICIAKTQ YSLSHDATLK GVPTGFTFPI 900
    RDVRLSNGAG YLYALAAEIQ TIPGLATYAG YMAVEVDDDG EIDGLF 946
    Length:946
    Mass (Da):102,205
    Last modified:April 1, 1988 - v1
    Checksum:iBC2897EC380CBA6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12878 Genomic DNA. Translation: AAA66316.1.
    Z49133 Genomic DNA. Translation: CAA88997.1.
    Z72989 Genomic DNA. Translation: CAA97231.1.
    AY692966 Genomic DNA. Translation: AAT92985.1.
    BK006941 Genomic DNA. Translation: DAA08297.1.
    PIRiA29550.
    RefSeqiNP_011720.3. NM_001181333.3.

    Genome annotation databases

    EnsemblFungiiYGR204W; YGR204W; YGR204W.
    GeneIDi853118.
    KEGGisce:YGR204W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12878 Genomic DNA. Translation: AAA66316.1 .
    Z49133 Genomic DNA. Translation: CAA88997.1 .
    Z72989 Genomic DNA. Translation: CAA97231.1 .
    AY692966 Genomic DNA. Translation: AAT92985.1 .
    BK006941 Genomic DNA. Translation: DAA08297.1 .
    PIRi A29550.
    RefSeqi NP_011720.3. NM_001181333.3.

    3D structure databases

    ProteinModelPortali P07245.
    SMRi P07245. Positions 2-297, 320-946.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33457. 33 interactions.
    DIPi DIP-3867N.
    IntActi P07245. 11 interactions.
    MINTi MINT-574376.
    STRINGi 4932.YGR204W.

    Proteomic databases

    MaxQBi P07245.
    PaxDbi P07245.
    PeptideAtlasi P07245.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR204W ; YGR204W ; YGR204W .
    GeneIDi 853118.
    KEGGi sce:YGR204W.

    Organism-specific databases

    CYGDi YGR204w.
    SGDi S000003436. ADE3.

    Phylogenomic databases

    eggNOGi COG0190.
    GeneTreei ENSGT00750000117401.
    HOGENOMi HOG000040280.
    KOi K00288.
    OMAi HEYLDEN.
    OrthoDBi EOG7K0ZMJ.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci YEAST:YGR204W-MONOMER.

    Miscellaneous databases

    NextBioi 973148.

    Gene expression databases

    Genevestigatori P07245.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    3.40.50.720. 1 hit.
    HAMAPi MF_01543. FTHFS.
    MF_01576. THF_DHG_CYH.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020867. THF_DH/CycHdrlase_CS.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00085. THFDHDRGNASE.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    PS00766. THF_DHG_CYH_1. 1 hit.
    PS00767. THF_DHG_CYH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Saccharomyces cerevisiae ADE3 gene encoding C1-tetrahydrofolate synthase."
      Staben C., Rabinowitz J.C.
      J. Biol. Chem. 261:4629-4637(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs."
      Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C.
      Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Site-directed mutagenesis of yeast C1-tetrahydrofolate synthase: analysis of an overlapping active site in a multifunctional enzyme."
      Barlowe C.K., Williams M.E., Rabinowitz J.C., Appling D.R.
      Biochemistry 28:2099-2106(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176; THR-318 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiC1TC_YEAST
    AccessioniPrimary (citable) accession number: P07245
    Secondary accession number(s): D6VUY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 150 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 35600 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3