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Protein

C-1-tetrahydrofolate synthase, cytoplasmic

Gene

ADE3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 35600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi169 – 171NADPBy similarity3
Nucleotide bindingi384 – 391ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • formate-tetrahydrofolate ligase activity Source: SGD
  • methenyltetrahydrofolate cyclohydrolase activity Source: SGD
  • methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Multifunctional enzyme, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YGR204W-MONOMER
BRENDAi3.5.4.9 984
6.3.4.3 984
ReactomeiR-SCE-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, cytoplasmic
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
Gene namesi
Name:ADE3
Ordered Locus Names:YGR204W
ORF Names:G7733
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR204W
SGDiS000003436 ADE3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11C → S: Almost no effect on activity. 1 Publication1
Mutagenesisi144C → S: Cyclohydrolase activity is reduced 20-fold. Dehydrogenase Km is increased 7-fold. 1 Publication1
Mutagenesisi257C → S: Cyclohydrolase activity is increased 2-fold. Dehydrogenase Km is increased 2-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001993261 – 946C-1-tetrahydrofolate synthase, cytoplasmicAdd BLAST946

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176PhosphoserineCombined sources1
Modified residuei318PhosphothreonineCombined sources1
Modified residuei322PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07245
PaxDbiP07245
PRIDEiP07245

PTM databases

iPTMnetiP07245

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi33457, 56 interactors
DIPiDIP-3867N
IntActiP07245, 14 interactors
MINTiP07245
STRINGi4932.YGR204W

Structurei

3D structure databases

ProteinModelPortaliP07245
SMRiP07245
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 319Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST319
Regioni51 – 55Substrate bindingBy similarity5
Regioni98 – 100Substrate bindingBy similarity3
Regioni277 – 281Substrate bindingBy similarity5
Regioni320 – 946Formyltetrahydrofolate synthetaseAdd BLAST627

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140894
HOGENOMiHOG000040280
InParanoidiP07245
KOiK00288
OMAiCGEIMTM
OrthoDBiEOG092C0B4M

Family and domain databases

CDDicd00477 FTHFS, 1 hit
HAMAPiMF_01543 FTHFS, 1 hit
MF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR000559 Formate_THF_ligase
IPR020628 Formate_THF_ligase_CS
IPR036291 NAD(P)-bd_dom_sf
IPR027417 P-loop_NTPase
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020867 THF_DH/CycHdrlase_CS
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PfamiView protein in Pfam
PF01268 FTHFS, 1 hit
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00721 FTHFS_1, 1 hit
PS00722 FTHFS_2, 1 hit
PS00766 THF_DHG_CYH_1, 1 hit
PS00767 THF_DHG_CYH_2, 1 hit

Sequencei

Sequence statusi: Complete.

P07245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQVLDGKA CAQQFRSNIA NEIKSIQGHV PGFAPNLAII QVGNRPDSAT
60 70 80 90 100
YVRMKRKAAE EAGIVANFIH LDESATEFEV LRYVDQLNED PHTHGIIVQL
110 120 130 140 150
PLPAHLDEDR ITSRVLAEKD VDGFGPTNIG ELNKKNGHPF FLPCTPKGII
160 170 180 190 200
ELLHKANVTI EGSRSVVIGR SDIVGSPVAE LLKSLNSTVT ITHSKTRDIA
210 220 230 240 250
SYLHDADIVV VAIGQPEFVK GEWFKPRDGT SSDKKTVVID VGTNYVADPS
260 270 280 290 300
KKSGFKCVGD VEFNEAIKYV HLITPVPGGV GPMTVAMLMQ NTLIAAKRQM
310 320 330 340 350
EESSKPLQIP PLPLKLLTPV PSDIDISRAQ QPKLINQLAQ ELGIYSHELE
360 370 380 390 400
LYGHYKAKIS PKVIERLQTR QNGKYILVSG ITPTPLGEGK STTTMGLVQA
410 420 430 440 450
LTAHLGKPAI ANVRQPSLGP TLGVKGGAAG GGYSQVIPMD EFNLHLTGDI
460 470 480 490 500
HAIGAANNLL AAAIDTRMFH ETTQKNDATF YNRLVPRKNG KRKFTPSMQR
510 520 530 540 550
RLNRLGIQKT NPDDLTPEEI NKFARLNIDP DTITIKRVVD INDRMLRQIT
560 570 580 590 600
IGQAPTEKNH TRVTGFDITV ASELMAILAL SKDLRDMKER IGRVVVAADV
610 620 630 640 650
NRSPVTVEDV GCTGALTALL RDAIKPNLMQ TLEGTPVLVH AGPFANISIG
660 670 680 690 700
ASSVIADRVA LKLVGTEPEA KTEAGYVVTE AGFDFTMGGE RFFNIKCRSS
710 720 730 740 750
GLTPNAVVLV ATVRALKSHG GAPDVKPGQP LPSAYTEENI EFVEKGAANM
760 770 780 790 800
CKQIANIKQF GVPVVVAINK FETDTEGEIA AIRKAALEAG AFEAVTSNHW
810 820 830 840 850
AEGGKGAIDL AKAVIEASNQ PVDFHFLYDV NSSVEDKLTT IVQKMYGGAA
860 870 880 890 900
IDILPEAQRK IDMYKEQGFG NLPICIAKTQ YSLSHDATLK GVPTGFTFPI
910 920 930 940
RDVRLSNGAG YLYALAAEIQ TIPGLATYAG YMAVEVDDDG EIDGLF
Length:946
Mass (Da):102,205
Last modified:April 1, 1988 - v1
Checksum:iBC2897EC380CBA6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12878 Genomic DNA Translation: AAA66316.1
Z49133 Genomic DNA Translation: CAA88997.1
Z72989 Genomic DNA Translation: CAA97231.1
AY692966 Genomic DNA Translation: AAT92985.1
BK006941 Genomic DNA Translation: DAA08297.1
PIRiA29550
RefSeqiNP_011720.3, NM_001181333.3

Genome annotation databases

EnsemblFungiiYGR204W; YGR204W; YGR204W
GeneIDi853118
KEGGisce:YGR204W

Similar proteinsi

Entry informationi

Entry nameiC1TC_YEAST
AccessioniPrimary (citable) accession number: P07245
Secondary accession number(s): D6VUY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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