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Protein

Bifunctional purine biosynthetic protein ADE5,7

Gene

ADE5,7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 35800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (ADE6)
  2. Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ADE4)
  2. Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298ManganeseBy similarity1
Metal bindingi300ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 203ATPBy similarityAdd BLAST64

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphoribosylamine-glycine ligase activity Source: SGD
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YGL234W-MONOMER
YEAST:YGL234W-MONOMER
ReactomeiR-SCE-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00125
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE5,7
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ADE5,7
Ordered Locus Names:YGL234W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL234W
SGDiS000003203 ADE5,7

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749411 – 802Bifunctional purine biosynthetic protein ADE5,7Add BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei455PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07244
PaxDbiP07244
PRIDEiP07244

PTM databases

iPTMnetiP07244

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GSY2P274723EBI-323,EBI-8036

Protein-protein interaction databases

BioGridi33005, 64 interactors
DIPiDIP-4080N
IntActiP07244, 42 interactors
MINTiP07244
STRINGi4932.YGL234W

Structurei

3D structure databases

ProteinModelPortaliP07244
SMRiP07244
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 330ATP-graspAdd BLAST217

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 450GARSAdd BLAST450
Regioni451 – 802AIRSAdd BLAST352

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000292
HOGENOMiHOG000030315
InParanoidiP07244
KOiK11788
OMAiLLERHNC
OrthoDBiEOG092C3N9W

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

P07244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNILVLGNG AREHVLVTKL AQSPTVGKIY VAPGNGGTAT MDPSRVINWD
60 70 80 90 100
ITPDVANFAR LQSMAVEHKI NLVVPGPELP LVNGITSVFH SVGIPVFGPS
110 120 130 140 150
VKAAQLEASK AFSKRFMSKH NIPTASYDVF TNPEEAISFL QAHTDKAFVI
160 170 180 190 200
KADGIAAGKG VIIPSSIDES VQAIKDIMVT KQFGEEAGKQ VVIEQFLEGD
210 220 230 240 250
EISLLTIVDG YSHFNLPVAQ DHKRIFDGDK GLNTGGMGAY APAPVATPSL
260 270 280 290 300
LKTIDSQIVK PTIDGMRRDG MPFVGVLFTG MILVKDSKTN QLVPEVLEYN
310 320 330 340 350
VRFGDPETQA VLSLLDDQTD LAQVFLAAAE HRLDSVNIGI DDTRSAVTVV
360 370 380 390 400
VAAGGYPESY AKGDKITLDT DKLPPHTQIF QAGTKYDSAT DSLLTNGGRV
410 420 430 440 450
LSVTSTAQDL RTAVDTVYEA VKCVHFQNSY YRKDIAYRAF QNSESSKVAI
460 470 480 490 500
TYADSGVSVD NGNNLVQTIK EMVRSTRRPG ADSDIGGFGG LFDLAQAGFR
510 520 530 540 550
QNEDTLLVGA TDGVGTKLII AQETGIHNTV GIDLVAMNVN DLVVQGAEPL
560 570 580 590 600
FFLDYFATGA LDIQVASDFV SGVANGCIQS GCALVGGETS EMPGMYPPGH
610 620 630 640 650
YDTNGTAVGA VLRQDILPKI NEMAAGDVLL GLASSGVHSN GFSLVRKIIQ
660 670 680 690 700
HVALPWDAPC PWDESKTLGE GILEPTKIYV KQLLPSIRQR LLLGLAHITG
710 720 730 740 750
GGLVENIPRA IPDHLQARVD MSTWEVPRVF KWFGQAGNVP HDDILRTFNM
760 770 780 790 800
GVGMVLIVKR ENVKAVCDSL TEEGEIIWEL GSLQERPKDA PGCVIENGTK

LY
Length:802
Mass (Da):86,068
Last modified:April 1, 1988 - v1
Checksum:i1583C6F3E64085D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28K → R in AAT93005 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04337 Genomic DNA Translation: CAA27867.1
Z72756 Genomic DNA Translation: CAA96952.1
AY692986 Genomic DNA Translation: AAT93005.1
BK006941 Genomic DNA Translation: DAA07884.1
PIRiA26343
RefSeqiNP_011280.1, NM_001181100.1

Genome annotation databases

EnsemblFungiiYGL234W; YGL234W; YGL234W
GeneIDi852617
KEGGisce:YGL234W

Similar proteinsi

Entry informationi

Entry nameiPUR2_YEAST
AccessioniPrimary (citable) accession number: P07244
Secondary accession number(s): D6VVA0, E9P907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health