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Protein

Bifunctional purine biosynthetic protein ADE5,7

Gene

ADE5,7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (ADE6)
  2. Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (ADE4)
  2. Bifunctional purine biosynthetic protein ADE5,7 (ADE5,7)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298ManganeseBy similarity1
Metal bindingi300ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 203ATPBy similarityAdd BLAST64

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphoribosylamine-glycine ligase activity Source: SGD
  • phosphoribosylformylglycinamidine cyclo-ligase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YGL234W-MONOMER.
YEAST:YGL234W-MONOMER.
ReactomeiR-SCE-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE5,7
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ADE5,7
Ordered Locus Names:YGL234W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL234W.
SGDiS000003203. ADE5,7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749411 – 802Bifunctional purine biosynthetic protein ADE5,7Add BLAST802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei455PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07244.
PRIDEiP07244.

PTM databases

iPTMnetiP07244.

Interactioni

Protein-protein interaction databases

BioGridi33005. 37 interactors.
DIPiDIP-4080N.
IntActiP07244. 15 interactors.
MINTiMINT-550925.

Structurei

3D structure databases

ProteinModelPortaliP07244.
SMRiP07244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 330ATP-graspAdd BLAST217

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 450GARSAdd BLAST450
Regioni451 – 802AIRSAdd BLAST352

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
InParanoidiP07244.
KOiK11788.
OMAiFPFVGML.
OrthoDBiEOG092C3N9W.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNILVLGNG AREHVLVTKL AQSPTVGKIY VAPGNGGTAT MDPSRVINWD
60 70 80 90 100
ITPDVANFAR LQSMAVEHKI NLVVPGPELP LVNGITSVFH SVGIPVFGPS
110 120 130 140 150
VKAAQLEASK AFSKRFMSKH NIPTASYDVF TNPEEAISFL QAHTDKAFVI
160 170 180 190 200
KADGIAAGKG VIIPSSIDES VQAIKDIMVT KQFGEEAGKQ VVIEQFLEGD
210 220 230 240 250
EISLLTIVDG YSHFNLPVAQ DHKRIFDGDK GLNTGGMGAY APAPVATPSL
260 270 280 290 300
LKTIDSQIVK PTIDGMRRDG MPFVGVLFTG MILVKDSKTN QLVPEVLEYN
310 320 330 340 350
VRFGDPETQA VLSLLDDQTD LAQVFLAAAE HRLDSVNIGI DDTRSAVTVV
360 370 380 390 400
VAAGGYPESY AKGDKITLDT DKLPPHTQIF QAGTKYDSAT DSLLTNGGRV
410 420 430 440 450
LSVTSTAQDL RTAVDTVYEA VKCVHFQNSY YRKDIAYRAF QNSESSKVAI
460 470 480 490 500
TYADSGVSVD NGNNLVQTIK EMVRSTRRPG ADSDIGGFGG LFDLAQAGFR
510 520 530 540 550
QNEDTLLVGA TDGVGTKLII AQETGIHNTV GIDLVAMNVN DLVVQGAEPL
560 570 580 590 600
FFLDYFATGA LDIQVASDFV SGVANGCIQS GCALVGGETS EMPGMYPPGH
610 620 630 640 650
YDTNGTAVGA VLRQDILPKI NEMAAGDVLL GLASSGVHSN GFSLVRKIIQ
660 670 680 690 700
HVALPWDAPC PWDESKTLGE GILEPTKIYV KQLLPSIRQR LLLGLAHITG
710 720 730 740 750
GGLVENIPRA IPDHLQARVD MSTWEVPRVF KWFGQAGNVP HDDILRTFNM
760 770 780 790 800
GVGMVLIVKR ENVKAVCDSL TEEGEIIWEL GSLQERPKDA PGCVIENGTK

LY
Length:802
Mass (Da):86,068
Last modified:April 1, 1988 - v1
Checksum:i1583C6F3E64085D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28K → R in AAT93005 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04337 Genomic DNA. Translation: CAA27867.1.
Z72756 Genomic DNA. Translation: CAA96952.1.
AY692986 Genomic DNA. Translation: AAT93005.1.
BK006941 Genomic DNA. Translation: DAA07884.1.
PIRiA26343.
RefSeqiNP_011280.1. NM_001181100.1.

Genome annotation databases

EnsemblFungiiYGL234W; YGL234W; YGL234W.
GeneIDi852617.
KEGGisce:YGL234W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04337 Genomic DNA. Translation: CAA27867.1.
Z72756 Genomic DNA. Translation: CAA96952.1.
AY692986 Genomic DNA. Translation: AAT93005.1.
BK006941 Genomic DNA. Translation: DAA07884.1.
PIRiA26343.
RefSeqiNP_011280.1. NM_001181100.1.

3D structure databases

ProteinModelPortaliP07244.
SMRiP07244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33005. 37 interactors.
DIPiDIP-4080N.
IntActiP07244. 15 interactors.
MINTiMINT-550925.

PTM databases

iPTMnetiP07244.

Proteomic databases

MaxQBiP07244.
PRIDEiP07244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL234W; YGL234W; YGL234W.
GeneIDi852617.
KEGGisce:YGL234W.

Organism-specific databases

EuPathDBiFungiDB:YGL234W.
SGDiS000003203. ADE5,7.

Phylogenomic databases

GeneTreeiENSGT00390000000292.
HOGENOMiHOG000030315.
InParanoidiP07244.
KOiK11788.
OMAiFPFVGML.
OrthoDBiEOG092C3N9W.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.
BioCyciMetaCyc:YGL234W-MONOMER.
YEAST:YGL234W-MONOMER.
ReactomeiR-SCE-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiP07244.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
MF_00138. GARS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SMARTiSM01210. GARS_C. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR2_YEAST
AccessioniPrimary (citable) accession number: P07244
Secondary accession number(s): D6VVA0, E9P907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 35800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.