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Protein

Dual specificity protein phosphatase H1

Gene

H1L

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein.5 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
Protein serine phosphate + H2O = protein serine + phosphate.

Kineticsi

  1. KM=87 µM for 3-O-methylfluorescein phosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei110Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

    Enzyme and pathway databases

    SABIO-RKP07239.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase H1 (EC:3.1.3.-, EC:3.1.3.48)
    Alternative name(s):
    Late protein H1
    Gene namesi
    ORF Names:H1L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    Proteomesi
    • UP000000344 Componenti: Genome

    Subcellular locationi

    • Virion 1 Publication
    • Host cytoplasm 1 Publication

    • Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi110C → S: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000948681 – 171Dual specificity protein phosphatase H1Add BLAST171

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    STAT1P422243EBI-7789600,EBI-1057697From a different organism.

    Protein-protein interaction databases

    IntActiP07239. 1 interactor.
    MINTiMINT-8216572.

    Structurei

    Secondary structure

    1171
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi6 – 14Combined sources9
    Beta strandi31 – 37Combined sources7
    Helixi39 – 43Combined sources5
    Helixi45 – 47Combined sources3
    Beta strandi48 – 50Combined sources3
    Beta strandi53 – 57Combined sources5
    Beta strandi59 – 61Combined sources3
    Beta strandi71 – 74Combined sources4
    Beta strandi80 – 82Combined sources3
    Helixi86 – 88Combined sources3
    Helixi89 – 102Combined sources14
    Beta strandi106 – 109Combined sources4
    Beta strandi111 – 115Combined sources5
    Helixi116 – 128Combined sources13
    Helixi134 – 149Combined sources16
    Helixi156 – 166Combined sources11

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2Q05X-ray2.57A/B/C/D1-171[»]
    2RF6X-ray1.95A1-171[»]
    3CM3X-ray1.32A1-171[»]
    ProteinModelPortaliP07239.
    SMRiP07239.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07239.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 27DimerizationAdd BLAST27

    Sequence similaritiesi

    Family and domain databases

    CDDicd00127. DSPc. 1 hit.
    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR016130. Tyr_Pase_AS.
    IPR003595. Tyr_Pase_cat.
    IPR000387. TYR_PHOSPHATASE_dom.
    IPR020422. TYR_PHOSPHATASE_DUAL_dom.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    SM00404. PTPc_motif. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07239-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDKKSLYKYL LLRSTGDMHK AKSPTIMTRV TNNVYLGNYK NAMDAPSSEV
    60 70 80 90 100
    KFKYVLNLTM DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD
    110 120 130 140 150
    QRNEPVLVHC AAGVNRSGAM ILAYLMSKNK ESLPMLYFLY VYHSMRDLRG
    160 170
    AFVENPSFKR QIIEKYVIDK N
    Length:171
    Mass (Da):19,698
    Last modified:April 1, 1988 - v1
    Checksum:i65DE6E875C7AD47E
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti20K → R in AAO89378 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1.
    AY243312 Genomic DNA. Translation: AAO89378.1.
    PIRiA24481. QQVZH1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1.
    AY243312 Genomic DNA. Translation: AAO89378.1.
    PIRiA24481. QQVZH1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2Q05X-ray2.57A/B/C/D1-171[»]
    2RF6X-ray1.95A1-171[»]
    3CM3X-ray1.32A1-171[»]
    ProteinModelPortaliP07239.
    SMRiP07239.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiP07239. 1 interactor.
    MINTiMINT-8216572.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    SABIO-RKP07239.

    Miscellaneous databases

    EvolutionaryTraceiP07239.

    Family and domain databases

    CDDicd00127. DSPc. 1 hit.
    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR016130. Tyr_Pase_AS.
    IPR003595. Tyr_Pase_cat.
    IPR000387. TYR_PHOSPHATASE_dom.
    IPR020422. TYR_PHOSPHATASE_DUAL_dom.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    SM00404. PTPc_motif. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDUSP_VACCW
    AccessioniPrimary (citable) accession number: P07239
    Secondary accession number(s): Q80HW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: November 30, 2016
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.