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Protein

Dual specificity protein phosphatase H1

Gene

H1L

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein.5 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
Protein serine phosphate + H2O = protein serine + phosphate.

Kineticsi

  1. KM=87 µM for 3-O-methylfluorescein phosphate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase H1 (EC:3.1.3.-, EC:3.1.3.48)
    Alternative name(s):
    Late protein H1
    Gene namesi
    ORF Names:H1L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344 Componenti: Genome

    Subcellular locationi

    • Virion 1 Publication
    • Host cytoplasm 1 Publication

    • Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi110 – 1101C → S: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 171171Dual specificity protein phosphatase H1PRO_0000094868Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    STAT1P422243EBI-7789600,EBI-1057697From a different organism.

    Protein-protein interaction databases

    IntActiP07239. 1 interaction.
    MINTiMINT-8216572.

    Structurei

    Secondary structure

    1
    171
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 149Combined sources
    Beta strandi31 – 377Combined sources
    Helixi39 – 435Combined sources
    Helixi45 – 473Combined sources
    Beta strandi48 – 503Combined sources
    Beta strandi53 – 575Combined sources
    Beta strandi59 – 613Combined sources
    Beta strandi71 – 744Combined sources
    Beta strandi80 – 823Combined sources
    Helixi86 – 883Combined sources
    Helixi89 – 10214Combined sources
    Beta strandi106 – 1094Combined sources
    Beta strandi111 – 1155Combined sources
    Helixi116 – 12813Combined sources
    Helixi134 – 14916Combined sources
    Helixi156 – 16611Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Q05X-ray2.57A/B/C/D1-171[»]
    2RF6X-ray1.95A1-171[»]
    3CM3X-ray1.32A1-171[»]
    ProteinModelPortaliP07239.
    SMRiP07239. Positions 1-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07239.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2727DimerizationAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07239-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDKKSLYKYL LLRSTGDMHK AKSPTIMTRV TNNVYLGNYK NAMDAPSSEV
    60 70 80 90 100
    KFKYVLNLTM DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD
    110 120 130 140 150
    QRNEPVLVHC AAGVNRSGAM ILAYLMSKNK ESLPMLYFLY VYHSMRDLRG
    160 170
    AFVENPSFKR QIIEKYVIDK N
    Length:171
    Mass (Da):19,698
    Last modified:April 1, 1988 - v1
    Checksum:i65DE6E875C7AD47E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201K → R in AAO89378 (Ref. 3) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1.
    AY243312 Genomic DNA. Translation: AAO89378.1.
    PIRiA24481. QQVZH1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1.
    AY243312 Genomic DNA. Translation: AAO89378.1.
    PIRiA24481. QQVZH1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Q05X-ray2.57A/B/C/D1-171[»]
    2RF6X-ray1.95A1-171[»]
    3CM3X-ray1.32A1-171[»]
    ProteinModelPortaliP07239.
    SMRiP07239. Positions 1-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiP07239. 1 interaction.
    MINTiMINT-8216572.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07239.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits."
      Broyles S.S., Moss B.
      Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment."
      Rosel J.L., Earl P.L., Weir J.P., Moss B.
      J. Virol. 60:436-449(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "A Tyr/Ser protein phosphatase encoded by vaccinia virus."
      Guan K., Broyels S.S., Dixon J.E.
      Nature 350:359-362(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ACTIVE SITE, MUTAGENESIS OF CYS-110.
    5. "Tyrosine phosphorylation of A17 during vaccinia virus infection: involvement of the H1 phosphatase and the F10 kinase."
      Derrien M., Punjabi A., Khanna M., Grubisha O., Traktman P.
      J. Virol. 73:7287-7296(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH A17.
    6. "Vaccinia virus blocks gamma interferon signal transduction: viral VH1 phosphatase reverses Stat1 activation."
      Najarro P., Traktman P., Lewis J.A.
      J. Virol. 75:3185-3196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Vaccinia virus blocks Stat1-dependent and Stat1-independent gene expression induced by type I and type II interferons."
      Mann B.A., Huang J.H., Li P., Chang H.C., Slee R.B., O'Sullivan A., Anita M., Yeh N., Klemsz M.J., Brutkiewicz R.R., Blum J.S., Kaplan M.H.
      J. Interferon Cytokine Res. 28:367-380(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    8. "Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701."
      Koksal A.C., Cingolani G.
      J. Biol. Chem. 286:14373-14382(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    9. "Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1."
      Koksal A.C., Nardozzi J.D., Cingolani G.
      J. Biol. Chem. 284:10129-10137(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.32 ANGSTROMS).

    Entry informationi

    Entry nameiDUSP_VACCW
    AccessioniPrimary (citable) accession number: P07239
    Secondary accession number(s): Q80HW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: January 7, 2015
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.