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P07239

- DUSP_VACCW

UniProt

P07239 - DUSP_VACCW

Protein

Dual specificity protein phosphatase H1

Gene

H1L

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated host STAT1 and blocking interferon (IFN)-stimulated innate immune responses. Dephosphorylates the A17 protein.5 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
    Protein serine phosphate + H2O = protein serine + phosphate.

    Kineticsi

    1. KM=87 µM for 3-O-methylfluorescein phosphate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101Phosphocysteine intermediate1 PublicationPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
    3. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. suppression by virus of host STAT1 activity Source: UniProtKB-KW
    2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase H1 (EC:3.1.3.-, EC:3.1.3.48)
    Alternative name(s):
    Late protein H1
    Gene namesi
    ORF Names:H1L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    Subcellular locationi

    Virion 1 Publication. Host cytoplasm 1 Publication
    Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi110 – 1101C → S: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 171171Dual specificity protein phosphatase H1PRO_0000094868Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    STAT1P422243EBI-7789600,EBI-1057697From a different organism.

    Protein-protein interaction databases

    IntActiP07239. 1 interaction.
    MINTiMINT-8216572.

    Structurei

    Secondary structure

    1
    171
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 149
    Beta strandi31 – 377
    Helixi39 – 435
    Helixi45 – 473
    Beta strandi48 – 503
    Beta strandi53 – 575
    Beta strandi59 – 613
    Beta strandi71 – 744
    Beta strandi80 – 823
    Helixi86 – 883
    Helixi89 – 10214
    Beta strandi106 – 1094
    Beta strandi111 – 1155
    Helixi116 – 12813
    Helixi134 – 14916
    Helixi156 – 16611

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Q05X-ray2.57A/B/C/D1-171[»]
    2RF6X-ray1.95A1-171[»]
    3CM3X-ray1.32A1-171[»]
    ProteinModelPortaliP07239.
    SMRiP07239. Positions 1-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07239.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2727DimerizationAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07239-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKKSLYKYL LLRSTGDMHK AKSPTIMTRV TNNVYLGNYK NAMDAPSSEV    50
    KFKYVLNLTM DKYTLPNSNI NIIHIPLVDD TTTDISKYFD DVTAFLSKCD 100
    QRNEPVLVHC AAGVNRSGAM ILAYLMSKNK ESLPMLYFLY VYHSMRDLRG 150
    AFVENPSFKR QIIEKYVIDK N 171
    Length:171
    Mass (Da):19,698
    Last modified:April 1, 1988 - v1
    Checksum:i65DE6E875C7AD47E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201K → R in AAO89378. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1.
    AY243312 Genomic DNA. Translation: AAO89378.1.
    PIRiA24481. QQVZH1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13209 Genomic DNA. Translation: AAB59836.1 .
    AY243312 Genomic DNA. Translation: AAO89378.1 .
    PIRi A24481. QQVZH1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Q05 X-ray 2.57 A/B/C/D 1-171 [» ]
    2RF6 X-ray 1.95 A 1-171 [» ]
    3CM3 X-ray 1.32 A 1-171 [» ]
    ProteinModelPortali P07239.
    SMRi P07239. Positions 1-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P07239. 1 interaction.
    MINTi MINT-8216572.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P07239.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits."
      Broyles S.S., Moss B.
      Proc. Natl. Acad. Sci. U.S.A. 83:3141-3145(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment."
      Rosel J.L., Earl P.L., Weir J.P., Moss B.
      J. Virol. 60:436-449(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "A Tyr/Ser protein phosphatase encoded by vaccinia virus."
      Guan K., Broyels S.S., Dixon J.E.
      Nature 350:359-362(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ACTIVE SITE, MUTAGENESIS OF CYS-110.
    5. "Tyrosine phosphorylation of A17 during vaccinia virus infection: involvement of the H1 phosphatase and the F10 kinase."
      Derrien M., Punjabi A., Khanna M., Grubisha O., Traktman P.
      J. Virol. 73:7287-7296(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH A17.
    6. "Vaccinia virus blocks gamma interferon signal transduction: viral VH1 phosphatase reverses Stat1 activation."
      Najarro P., Traktman P., Lewis J.A.
      J. Virol. 75:3185-3196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Vaccinia virus blocks Stat1-dependent and Stat1-independent gene expression induced by type I and type II interferons."
      Mann B.A., Huang J.H., Li P., Chang H.C., Slee R.B., O'Sullivan A., Anita M., Yeh N., Klemsz M.J., Brutkiewicz R.R., Blum J.S., Kaplan M.H.
      J. Interferon Cytokine Res. 28:367-380(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    8. "Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701."
      Koksal A.C., Cingolani G.
      J. Biol. Chem. 286:14373-14382(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    9. "Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1."
      Koksal A.C., Nardozzi J.D., Cingolani G.
      J. Biol. Chem. 284:10129-10137(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.32 ANGSTROMS).

    Entry informationi

    Entry nameiDUSP_VACCW
    AccessioniPrimary (citable) accession number: P07239
    Secondary accession number(s): Q80HW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3