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Protein

Threonine--tRNA ligase, mitochondrial

Gene

MST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • RNA binding Source: GO_Central
  • threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  • mitochondrial threonyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31956-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, mitochondrial (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:MST1
Ordered Locus Names:YKL194C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL194C.
SGDiS000001677. MST1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45MitochondrionSequence analysisAdd BLAST45
ChainiPRO_000003582746 – 462Threonine--tRNA ligase, mitochondrialAdd BLAST417

Proteomic databases

MaxQBiP07236.
PRIDEiP07236.

Interactioni

Protein-protein interaction databases

BioGridi33927. 25 interactors.
DIPiDIP-4855N.
IntActiP07236. 1 interactor.
MINTiMINT-555767.

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 46Combined sources12
Helixi63 – 82Combined sources20
Beta strandi93 – 96Combined sources4
Helixi97 – 102Combined sources6
Turni106 – 108Combined sources3
Helixi110 – 112Combined sources3
Beta strandi113 – 117Combined sources5
Beta strandi120 – 122Combined sources3
Beta strandi126 – 128Combined sources3
Helixi133 – 140Combined sources8
Helixi147 – 149Combined sources3
Beta strandi151 – 156Combined sources6
Beta strandi159 – 161Combined sources3
Turni166 – 168Combined sources3
Turni171 – 173Combined sources3
Beta strandi176 – 187Combined sources12
Helixi189 – 191Combined sources3
Helixi192 – 207Combined sources16
Turni208 – 210Combined sources3
Beta strandi225 – 229Combined sources5
Helixi239 – 256Combined sources18
Beta strandi260 – 263Combined sources4
Beta strandi273 – 279Combined sources7
Beta strandi285 – 295Combined sources11
Helixi296 – 300Combined sources5
Beta strandi316 – 325Combined sources10
Helixi326 – 337Combined sources12
Helixi343 – 345Combined sources3
Beta strandi350 – 356Combined sources7
Helixi360 – 374Combined sources15
Beta strandi379 – 381Combined sources3
Beta strandi394 – 396Combined sources3
Helixi403 – 413Combined sources11
Beta strandi416 – 421Combined sources6
Helixi423 – 428Combined sources6
Beta strandi431 – 435Combined sources5
Helixi436 – 438Combined sources3
Beta strandi443 – 445Combined sources3
Helixi447 – 459Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
4YYEX-ray2.30A/B26-462[»]
ProteinModelPortaliP07236.
SMRiP07236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000003878.
InParanoidiP07236.
KOiK01868.
OMAiYKRPIMI.
OrthoDBiEOG092C32CL.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR033728. ThrRS_core.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM
60 70 80 90 100
TDPLSPGSMF FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW
110 120 130 140 150
EKSGHWENYA DDMFKVETTD EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL
160 170 180 190 200
PLRFSDFSPL HRNEASGALS GLTRLRKFHQ DDGHIFCTPS QVKSEIFNSL
210 220 230 240 250
KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK VWNHAEQVLK
260 270 280 290 300
EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER
310 320 330 340 350
FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA
360 370 380 390 400
VIIPVNTKNV QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN
410 420 430 440 450
EPVGYRIKSA ILKNYSYLII VGDEEVQLQK YNIRERDNRK SFEKLTMSQI
460
WEKFIELEKN YK
Length:462
Mass (Da):54,092
Last modified:June 1, 1994 - v2
Checksum:iBC90DED57EB037BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265G → S in AAA34808 (PubMed:2999113).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRiS38031. YSBYTM.
RefSeqiNP_012727.1. NM_001179760.1.

Genome annotation databases

EnsemblFungiiYKL194C; YKL194C; YKL194C.
GeneIDi853640.
KEGGisce:YKL194C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRiS38031. YSBYTM.
RefSeqiNP_012727.1. NM_001179760.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
4YYEX-ray2.30A/B26-462[»]
ProteinModelPortaliP07236.
SMRiP07236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33927. 25 interactors.
DIPiDIP-4855N.
IntActiP07236. 1 interactor.
MINTiMINT-555767.

Proteomic databases

MaxQBiP07236.
PRIDEiP07236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL194C; YKL194C; YKL194C.
GeneIDi853640.
KEGGisce:YKL194C.

Organism-specific databases

EuPathDBiFungiDB:YKL194C.
SGDiS000001677. MST1.

Phylogenomic databases

HOGENOMiHOG000003878.
InParanoidiP07236.
KOiK01868.
OMAiYKRPIMI.
OrthoDBiEOG092C32CL.

Enzyme and pathway databases

BioCyciYEAST:G3O-31956-MONOMER.

Miscellaneous databases

PROiP07236.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
IPR033728. ThrRS_core.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYTM_YEAST
AccessioniPrimary (citable) accession number: P07236
Secondary accession number(s): D6VX06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.