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Protein

Threonine--tRNA ligase, mitochondrial

Gene

MST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  • mitochondrial threonyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31956-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, mitochondrial (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:MST1
Ordered Locus Names:YKL194C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKL194c.
EuPathDBiFungiDB:YKL194C.
SGDiS000001677. MST1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545MitochondrionSequence AnalysisAdd
BLAST
Chaini46 – 462417Threonine--tRNA ligase, mitochondrialPRO_0000035827Add
BLAST

Proteomic databases

MaxQBiP07236.
PaxDbiP07236.
PeptideAtlasiP07236.

Interactioni

Protein-protein interaction databases

BioGridi33927. 24 interactions.
DIPiDIP-4855N.
IntActiP07236. 1 interaction.
MINTiMINT-555767.
STRINGi4932.YKL194C.

Structurei

Secondary structure

1
462
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4612Combined sources
Helixi63 – 8220Combined sources
Beta strandi93 – 964Combined sources
Helixi97 – 1026Combined sources
Turni106 – 1083Combined sources
Helixi110 – 1123Combined sources
Beta strandi113 – 1175Combined sources
Beta strandi120 – 1223Combined sources
Beta strandi126 – 1283Combined sources
Helixi133 – 1408Combined sources
Helixi147 – 1493Combined sources
Beta strandi151 – 1566Combined sources
Beta strandi159 – 1613Combined sources
Turni166 – 1683Combined sources
Turni171 – 1733Combined sources
Beta strandi176 – 18712Combined sources
Helixi189 – 1913Combined sources
Helixi192 – 20716Combined sources
Turni208 – 2103Combined sources
Beta strandi225 – 2295Combined sources
Helixi239 – 25618Combined sources
Beta strandi260 – 2634Combined sources
Beta strandi273 – 2797Combined sources
Beta strandi285 – 29511Combined sources
Helixi296 – 3005Combined sources
Beta strandi316 – 32510Combined sources
Helixi326 – 33712Combined sources
Helixi343 – 3453Combined sources
Beta strandi350 – 3567Combined sources
Helixi360 – 37415Combined sources
Beta strandi379 – 3813Combined sources
Beta strandi394 – 3963Combined sources
Helixi403 – 41311Combined sources
Beta strandi416 – 4216Combined sources
Helixi423 – 4286Combined sources
Beta strandi431 – 4355Combined sources
Helixi436 – 4383Combined sources
Beta strandi443 – 4453Combined sources
Helixi447 – 45913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
ProteinModelPortaliP07236.
SMRiP07236. Positions 36-462.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003878.
InParanoidiP07236.
KOiK01868.
OMAiCIKMMYD.
OrthoDBiEOG7KDFK9.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM
60 70 80 90 100
TDPLSPGSMF FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW
110 120 130 140 150
EKSGHWENYA DDMFKVETTD EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL
160 170 180 190 200
PLRFSDFSPL HRNEASGALS GLTRLRKFHQ DDGHIFCTPS QVKSEIFNSL
210 220 230 240 250
KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK VWNHAEQVLK
260 270 280 290 300
EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER
310 320 330 340 350
FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA
360 370 380 390 400
VIIPVNTKNV QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN
410 420 430 440 450
EPVGYRIKSA ILKNYSYLII VGDEEVQLQK YNIRERDNRK SFEKLTMSQI
460
WEKFIELEKN YK
Length:462
Mass (Da):54,092
Last modified:June 1, 1994 - v2
Checksum:iBC90DED57EB037BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651G → S in AAA34808 (PubMed:2999113).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRiS38031. YSBYTM.
RefSeqiNP_012727.1. NM_001179760.1.

Genome annotation databases

EnsemblFungiiYKL194C; YKL194C; YKL194C.
GeneIDi853640.
KEGGisce:YKL194C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRiS38031. YSBYTM.
RefSeqiNP_012727.1. NM_001179760.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
ProteinModelPortaliP07236.
SMRiP07236. Positions 36-462.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33927. 24 interactions.
DIPiDIP-4855N.
IntActiP07236. 1 interaction.
MINTiMINT-555767.
STRINGi4932.YKL194C.

Proteomic databases

MaxQBiP07236.
PaxDbiP07236.
PeptideAtlasiP07236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL194C; YKL194C; YKL194C.
GeneIDi853640.
KEGGisce:YKL194C.

Organism-specific databases

CYGDiYKL194c.
EuPathDBiFungiDB:YKL194C.
SGDiS000001677. MST1.

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003878.
InParanoidiP07236.
KOiK01868.
OMAiCIKMMYD.
OrthoDBiEOG7KDFK9.

Enzyme and pathway databases

BioCyciYEAST:G3O-31956-MONOMER.

Miscellaneous databases

NextBioi974531.
PROiP07236.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase."
    Pape L.K., Koerner T.J., Tzagoloff A.
    J. Biol. Chem. 260:15362-15370(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase."
    Mackelvie S.H., Andrews P.D., Stark M.J.R.
    Mol. Cell. Biol. 15:3777-3785(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
    Strain: ATCC 200358 / YNN 295.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYTM_YEAST
AccessioniPrimary (citable) accession number: P07236
Secondary accession number(s): D6VX06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 1, 1994
Last modified: June 24, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.