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P07236

- SYTM_YEAST

UniProt

P07236 - SYTM_YEAST

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Protein
Threonine--tRNA ligase, mitochondrial
Gene
MST1, YKL194C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. threonine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. mitochondrial threonyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31956-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, mitochondrial (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:MST1
Ordered Locus Names:YKL194C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL194c.
SGDiS000001677. MST1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545Mitochondrion Reviewed prediction
Add
BLAST
Chaini46 – 462417Threonine--tRNA ligase, mitochondrial
PRO_0000035827Add
BLAST

Proteomic databases

MaxQBiP07236.
PaxDbiP07236.
PeptideAtlasiP07236.

Expressioni

Gene expression databases

GenevestigatoriP07236.

Interactioni

Protein-protein interaction databases

BioGridi33927. 24 interactions.
DIPiDIP-4855N.
IntActiP07236. 1 interaction.
MINTiMINT-555767.
STRINGi4932.YKL194C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4612
Helixi63 – 8220
Beta strandi93 – 964
Helixi97 – 1026
Turni106 – 1083
Helixi110 – 1123
Beta strandi113 – 1175
Beta strandi120 – 1223
Beta strandi126 – 1283
Helixi133 – 1408
Helixi147 – 1493
Beta strandi151 – 1566
Beta strandi159 – 1613
Turni166 – 1683
Turni171 – 1733
Beta strandi176 – 18712
Helixi189 – 1913
Helixi192 – 20716
Turni208 – 2103
Beta strandi225 – 2295
Helixi239 – 25618
Beta strandi260 – 2634
Beta strandi273 – 2797
Beta strandi285 – 29511
Helixi296 – 3005
Beta strandi316 – 32510
Helixi326 – 33712
Helixi343 – 3453
Beta strandi350 – 3567
Helixi360 – 37415
Beta strandi379 – 3813
Beta strandi394 – 3963
Helixi403 – 41311
Beta strandi416 – 4216
Helixi423 – 4286
Beta strandi431 – 4355
Helixi436 – 4383
Beta strandi443 – 4453
Helixi447 – 45913

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
ProteinModelPortaliP07236.
SMRiP07236. Positions 36-462.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003878.
KOiK01868.
OMAiCIICHSS.
OrthoDBiEOG7KDFK9.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07236-1 [UniParc]FASTAAdd to Basket

« Hide

MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM    50
TDPLSPGSMF FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW 100
EKSGHWENYA DDMFKVETTD EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL 150
PLRFSDFSPL HRNEASGALS GLTRLRKFHQ DDGHIFCTPS QVKSEIFNSL 200
KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK VWNHAEQVLK 250
EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER 300
FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA 350
VIIPVNTKNV QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN 400
EPVGYRIKSA ILKNYSYLII VGDEEVQLQK YNIRERDNRK SFEKLTMSQI 450
WEKFIELEKN YK 462
Length:462
Mass (Da):54,092
Last modified:June 1, 1994 - v2
Checksum:iBC90DED57EB037BF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651G → S in AAA34808. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRiS38031. YSBYTM.
RefSeqiNP_012727.1. NM_001179760.1.

Genome annotation databases

EnsemblFungiiYKL194C; YKL194C; YKL194C.
GeneIDi853640.
KEGGisce:YKL194C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12087 mRNA. Translation: AAA34808.1 .
Z28194 Genomic DNA. Translation: CAA82038.1 .
X83609 Genomic DNA. Translation: CAA58589.1 .
BK006944 Genomic DNA. Translation: DAA08972.1 .
PIRi S38031. YSBYTM.
RefSeqi NP_012727.1. NM_001179760.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3UGQ X-ray 2.10 A 26-462 [» ]
3UGT X-ray 3.60 A/B/C/D 26-462 [» ]
3UH0 X-ray 2.00 A 26-462 [» ]
4EO4 X-ray 2.87 A/B/C/D 26-462 [» ]
ProteinModelPortali P07236.
SMRi P07236. Positions 36-462.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33927. 24 interactions.
DIPi DIP-4855N.
IntActi P07236. 1 interaction.
MINTi MINT-555767.
STRINGi 4932.YKL194C.

Proteomic databases

MaxQBi P07236.
PaxDbi P07236.
PeptideAtlasi P07236.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL194C ; YKL194C ; YKL194C .
GeneIDi 853640.
KEGGi sce:YKL194C.

Organism-specific databases

CYGDi YKL194c.
SGDi S000001677. MST1.

Phylogenomic databases

eggNOGi COG0441.
HOGENOMi HOG000003878.
KOi K01868.
OMAi CIICHSS.
OrthoDBi EOG7KDFK9.

Enzyme and pathway databases

BioCyci YEAST:G3O-31956-MONOMER.

Miscellaneous databases

NextBioi 974531.

Gene expression databases

Genevestigatori P07236.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
[Graphical view ]
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view ]
PRINTSi PR01047. TRNASYNTHTHR.
SUPFAMi SSF52954. SSF52954. 1 hit.
TIGRFAMsi TIGR00418. thrS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase."
    Pape L.K., Koerner T.J., Tzagoloff A.
    J. Biol. Chem. 260:15362-15370(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase."
    Mackelvie S.H., Andrews P.D., Stark M.J.R.
    Mol. Cell. Biol. 15:3777-3785(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
    Strain: ATCC 200358 / YNN 295.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYTM_YEAST
AccessioniPrimary (citable) accession number: P07236
Secondary accession number(s): D6VX06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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