Reviewed, UniProtKB/Swiss-Prot P07236 (SYTM_YEAST)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase, mitochondrial
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	MST1
Ordered Locus Names:	YKL194C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 1240 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial threonyl-tRNA aminoacylation Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Ref.1 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Mitochondrion Potential
	Chain	46 – 462	417	Threonyl-tRNA synthetase, mitochondrial		PRO_0000035827
Experimental info
	Sequence conflict	265	1	G → S in AAA34808. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07236-1 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: BC90DED57EB037BF	FASTA	462	54,092
	10 20 30 40 50 60 MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM TDPLSPGSMF 70 80 90 100 110 120 FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW EKSGHWENYA DDMFKVETTD 130 140 150 160 170 180 EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL PLRFSDFSPL HRNEASGALS GLTRLRKFHQ 190 200 210 220 230 240 DDGHIFCTPS QVKSEIFNSL KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK 250 260 270 280 290 300 VWNHAEQVLK EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER 310 320 330 340 350 360 FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA VIIPVNTKNV 370 380 390 400 410 420 QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN EPVGYRIKSA ILKNYSYLII 430 440 450 460 VGDEEVQLQK YNIRERDNRK SFEKLTMSQI WEKFIELEKN YK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase." Pape L.K., Koerner T.J., Tzagoloff A. J. Biol. Chem. 260:15362-15370(1985) [PubMed: 2999113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase." Mackelvie S.H., Andrews P.D., Stark M.J.R. Mol. Cell. Biol. 15:3777-3785(1995) [PubMed: 7791785] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14. Strain: ATCC 200358 / YNN 295.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12087 mRNA. Translation: AAA34808.1. Z28194 Genomic DNA. Translation: CAA82038.1. X83609 Genomic DNA. Translation: CAA58589.1.
PIR	YSBYTM. S38031.
RefSeq	NP_012727.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P00955.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4855N.
IntAct	P07236. 3 interactions.
STRING	P07236.
Proteomic databases
PeptideAtlas	P07236.
Genome annotation databases
Ensembl	YKL194C; YKL194C; YKL194C; Saccharomyces cerevisiae. [Genome view]
GeneID	853640.
GenomeReviews	Gene locus YKL194C in contig Y13137_GR.
KEGG	sce:YKL194C.
NMPDR	fig|4932.3.peg.3704.
Organism-specific databases
CYGD	YKL194c.
SGD	S000001677. MST1.
Phylogenomic databases
HOGENOM	P07236.
OMA	LCPHHAL.
Enzyme and pathway databases
BRENDA	6.1.1.3. 250.
Gene expression databases
ArrayExpress	P07236.
Genevestigator	P07236.
GermOnline	YKL194C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974531.

Entry information

Entry name

SYTM_YEAST

Accession

Primary (citable) accession number: P07236

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 1, 1994
Last modified:	November 3, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)