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P07236

- SYTM_YEAST

UniProt

P07236 - SYTM_YEAST

Protein

Threonine--tRNA ligase, mitochondrial

Gene

MST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. threonine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. mitochondrial threonyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31956-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Threonine--tRNA ligase, mitochondrial (EC:6.1.1.3)
    Alternative name(s):
    Threonyl-tRNA synthetase
    Short name:
    ThrRS
    Gene namesi
    Name:MST1
    Ordered Locus Names:YKL194C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL194c.
    SGDiS000001677. MST1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545MitochondrionSequence AnalysisAdd
    BLAST
    Chaini46 – 462417Threonine--tRNA ligase, mitochondrialPRO_0000035827Add
    BLAST

    Proteomic databases

    MaxQBiP07236.
    PaxDbiP07236.
    PeptideAtlasiP07236.

    Expressioni

    Gene expression databases

    GenevestigatoriP07236.

    Interactioni

    Protein-protein interaction databases

    BioGridi33927. 24 interactions.
    DIPiDIP-4855N.
    IntActiP07236. 1 interaction.
    MINTiMINT-555767.
    STRINGi4932.YKL194C.

    Structurei

    Secondary structure

    1
    462
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 4612
    Helixi63 – 8220
    Beta strandi93 – 964
    Helixi97 – 1026
    Turni106 – 1083
    Helixi110 – 1123
    Beta strandi113 – 1175
    Beta strandi120 – 1223
    Beta strandi126 – 1283
    Helixi133 – 1408
    Helixi147 – 1493
    Beta strandi151 – 1566
    Beta strandi159 – 1613
    Turni166 – 1683
    Turni171 – 1733
    Beta strandi176 – 18712
    Helixi189 – 1913
    Helixi192 – 20716
    Turni208 – 2103
    Beta strandi225 – 2295
    Helixi239 – 25618
    Beta strandi260 – 2634
    Beta strandi273 – 2797
    Beta strandi285 – 29511
    Helixi296 – 3005
    Beta strandi316 – 32510
    Helixi326 – 33712
    Helixi343 – 3453
    Beta strandi350 – 3567
    Helixi360 – 37415
    Beta strandi379 – 3813
    Beta strandi394 – 3963
    Helixi403 – 41311
    Beta strandi416 – 4216
    Helixi423 – 4286
    Beta strandi431 – 4355
    Helixi436 – 4383
    Beta strandi443 – 4453
    Helixi447 – 45913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UGQX-ray2.10A26-462[»]
    3UGTX-ray3.60A/B/C/D26-462[»]
    3UH0X-ray2.00A26-462[»]
    4EO4X-ray2.87A/B/C/D26-462[»]
    ProteinModelPortaliP07236.
    SMRiP07236. Positions 36-462.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0441.
    HOGENOMiHOG000003878.
    KOiK01868.
    OMAiCIICHSS.
    OrthoDBiEOG7KDFK9.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002320. Thr-tRNA-ligase_IIa.
    [Graphical view]
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PRINTSiPR01047. TRNASYNTHTHR.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    TIGRFAMsiTIGR00418. thrS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07236-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM    50
    TDPLSPGSMF FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW 100
    EKSGHWENYA DDMFKVETTD EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL 150
    PLRFSDFSPL HRNEASGALS GLTRLRKFHQ DDGHIFCTPS QVKSEIFNSL 200
    KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK VWNHAEQVLK 250
    EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER 300
    FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA 350
    VIIPVNTKNV QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN 400
    EPVGYRIKSA ILKNYSYLII VGDEEVQLQK YNIRERDNRK SFEKLTMSQI 450
    WEKFIELEKN YK 462
    Length:462
    Mass (Da):54,092
    Last modified:June 1, 1994 - v2
    Checksum:iBC90DED57EB037BF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti265 – 2651G → S in AAA34808. (PubMed:2999113)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12087 mRNA. Translation: AAA34808.1.
    Z28194 Genomic DNA. Translation: CAA82038.1.
    X83609 Genomic DNA. Translation: CAA58589.1.
    BK006944 Genomic DNA. Translation: DAA08972.1.
    PIRiS38031. YSBYTM.
    RefSeqiNP_012727.1. NM_001179760.1.

    Genome annotation databases

    EnsemblFungiiYKL194C; YKL194C; YKL194C.
    GeneIDi853640.
    KEGGisce:YKL194C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12087 mRNA. Translation: AAA34808.1 .
    Z28194 Genomic DNA. Translation: CAA82038.1 .
    X83609 Genomic DNA. Translation: CAA58589.1 .
    BK006944 Genomic DNA. Translation: DAA08972.1 .
    PIRi S38031. YSBYTM.
    RefSeqi NP_012727.1. NM_001179760.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3UGQ X-ray 2.10 A 26-462 [» ]
    3UGT X-ray 3.60 A/B/C/D 26-462 [» ]
    3UH0 X-ray 2.00 A 26-462 [» ]
    4EO4 X-ray 2.87 A/B/C/D 26-462 [» ]
    ProteinModelPortali P07236.
    SMRi P07236. Positions 36-462.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33927. 24 interactions.
    DIPi DIP-4855N.
    IntActi P07236. 1 interaction.
    MINTi MINT-555767.
    STRINGi 4932.YKL194C.

    Proteomic databases

    MaxQBi P07236.
    PaxDbi P07236.
    PeptideAtlasi P07236.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL194C ; YKL194C ; YKL194C .
    GeneIDi 853640.
    KEGGi sce:YKL194C.

    Organism-specific databases

    CYGDi YKL194c.
    SGDi S000001677. MST1.

    Phylogenomic databases

    eggNOGi COG0441.
    HOGENOMi HOG000003878.
    KOi K01868.
    OMAi CIICHSS.
    OrthoDBi EOG7KDFK9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31956-MONOMER.

    Miscellaneous databases

    NextBioi 974531.

    Gene expression databases

    Genevestigatori P07236.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002320. Thr-tRNA-ligase_IIa.
    [Graphical view ]
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    PRINTSi PR01047. TRNASYNTHTHR.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    TIGRFAMsi TIGR00418. thrS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase."
      Pape L.K., Koerner T.J., Tzagoloff A.
      J. Biol. Chem. 260:15362-15370(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase."
      Mackelvie S.H., Andrews P.D., Stark M.J.R.
      Mol. Cell. Biol. 15:3777-3785(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
      Strain: ATCC 200358 / YNN 295.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYTM_YEAST
    AccessioniPrimary (citable) accession number: P07236
    Secondary accession number(s): D6VX06
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1240 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3