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P07236 (SYTM_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase, mitochondrial

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:MST1
Ordered Locus Names:YKL194C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

Subcellular location

Mitochondrion matrix.

Miscellaneous

Present with 1240 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4545Mitochondrion Potential
Chain46 – 462417Threonine--tRNA ligase, mitochondrial
PRO_0000035827

Experimental info

Sequence conflict2651G → S in AAA34808. Ref.1

Secondary structure

........................................................................ 462
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07236 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: BC90DED57EB037BF

FASTA46254,092
        10         20         30         40         50         60 
MKIQLVRWHC SRNALWNRAF YSTRKATKNA SSATPATMTS MVSQRQDLFM TDPLSPGSMF 

        70         80         90        100        110        120 
FLPNGAKIFN KLIEFMKLQQ KFKFGFNEVV TPLIYKKTLW EKSGHWENYA DDMFKVETTD 

       130        140        150        160        170        180 
EEKEEYGLKP MNCPGHCLIF GKKDRSYNEL PLRFSDFSPL HRNEASGALS GLTRLRKFHQ 

       190        200        210        220        230        240 
DDGHIFCTPS QVKSEIFNSL KLIDIVYNKI FPFVKGGSGA ESNYFINFST RPDHFIGDLK 

       250        260        270        280        290        300 
VWNHAEQVLK EILEESGKPW KLNPGDGAFY GPKLDIMVTD HLRKTHQVAT IQLDFQLPER 

       310        320        330        340        350        360 
FDLKFKDQDN SYKRPIMIHR ATFGSIERFM ALLIDSNEGR WPFWLNPYQA VIIPVNTKNV 

       370        380        390        400        410        420 
QQLDMCTALQ KKLRNELEAD DMEPVPLNDW HFNVDLDIRN EPVGYRIKSA ILKNYSYLII 

       430        440        450        460 
VGDEEVQLQK YNIRERDNRK SFEKLTMSQI WEKFIELEKN YK 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a yeast nuclear gene (MST1) coding for the mitochondrial threonyl-tRNA1 synthetase."
Pape L.K., Koerner T.J., Tzagoloff A.
J. Biol. Chem. 260:15362-15370(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase."
Mackelvie S.H., Andrews P.D., Stark M.J.R.
Mol. Cell. Biol. 15:3777-3785(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
Strain: ATCC 200358 / YNN 295.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12087 mRNA. Translation: AAA34808.1.
Z28194 Genomic DNA. Translation: CAA82038.1.
X83609 Genomic DNA. Translation: CAA58589.1.
BK006944 Genomic DNA. Translation: DAA08972.1.
PIRYSBYTM. S38031.
RefSeqNP_012727.1. NM_001179760.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UGQX-ray2.10A26-462[»]
3UGTX-ray3.60A/B/C/D26-462[»]
3UH0X-ray2.00A26-462[»]
4EO4X-ray2.87A/B/C/D26-462[»]
ProteinModelPortalP07236.
SMRP07236. Positions 36-462.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33927. 24 interactions.
DIPDIP-4855N.
IntActP07236. 1 interaction.
MINTMINT-555767.
STRING4932.YKL194C.

Proteomic databases

MaxQBP07236.
PaxDbP07236.
PeptideAtlasP07236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL194C; YKL194C; YKL194C.
GeneID853640.
KEGGsce:YKL194C.

Organism-specific databases

CYGDYKL194c.
SGDS000001677. MST1.

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000003878.
KOK01868.
OMACIICHSS.
OrthoDBEOG7KDFK9.

Enzyme and pathway databases

BioCycYEAST:G3O-31956-MONOMER.

Gene expression databases

GenevestigatorP07236.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002320. Thr-tRNA-ligase_IIa.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SUPFAMSSF52954. SSF52954. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974531.

Entry information

Entry nameSYTM_YEAST
AccessionPrimary (citable) accession number: P07236
Secondary accession number(s): D6VX06
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries