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Protein

Conantokin-G

Gene
N/A
Organism
Conus geographus (Geography cone) (Nubecula geographus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conantokins inhibit N-methyl-D-aspartate (NMDA) receptors. This toxin is selective for the NR2B/GRIN2B subunit. Induces sleep-like symptoms in young mice and hyperactivity in older mice.1 Publication

Miscellaneous

The mature peptide does not contain cysteine residue.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei85Important for selectivity1
Metal bindingi90Divalent metal cation; via 4-carboxyglutamate1
Metal bindingi94Divalent metal cation; via 4-carboxyglutamate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Ionotropic glutamate receptor inhibitor, Neurotoxin, Postsynaptic neurotoxin, Toxin
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Conantokin-G
Short name:
Con-G
Alternative name(s):
CGX-1007
Conotoxin GV
Sleeper peptide
OrganismiConus geographus (Geography cone) (Nubecula geographus)
Taxonomic identifieri6491 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri1373. Conantokin-G precursor.
1353. Conantokin-G precursor (variant).

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Failed in phase II clinical trial. Was tested under the name CGX-1007 by Cognetix Inc. to treat convulsion and epilepsy.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85L → I: No loss of inhibition of NR1a/NR2B receptor; no inhibition of NR1a/NR2A receptor (as for the wild-type). 1 Publication1
Mutagenesisi85L → V: No loss of inhibition of NR1a/NR2B receptor; no inhibition of NR1a/NR2A receptor (as for the wild-type). 1 Publication1
Mutagenesisi85L → Y: Little loss of inhibition of NR1a/NR2B receptor; 70% of inhibition of NR1a/NR2A receptor. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
PropeptideiPRO_000003506022 – 80Add BLAST59
PeptideiPRO_000003506181 – 97Conantokin-GAdd BLAST17

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei834-carboxyglutamate1 Publication1
Modified residuei844-carboxyglutamate1 Publication1
Modified residuei874-carboxyglutamate1 Publication1
Modified residuei904-carboxyglutamate1 Publication1
Modified residuei944-carboxyglutamate1 Publication1
Modified residuei97Asparagine amide1 Publication1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Gamma-carboxyglutamic acid

Expressioni

Tissue specificityi

Expressed by the venom duct.Curated

Structurei

Secondary structure

1100
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi82 – 96Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AD7NMR-A81-97[»]
1AWYNMR-A81-97[»]
1ONUNMR-A81-97[»]
2DPQX-ray1.25A81-97[»]
ProteinModelPortaliP07231.
SMRiP07231.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07231.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 80Gamma-carboxylation recognition sequence that plays a role in the conversion of Glu to carboxy-Glu (Gla)CuratedAdd BLAST20

Sequence similaritiesi

Belongs to the conotoxin B superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR005918. Conantokin_CS.
PROSITEiView protein in PROSITE
PS60025. CONANTOKIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLYTYLYLL VPLVTFHLIL GTGTLDDGGA LTERRSADAT ALKAEPVLLQ
60 70 80 90 100
KSAARSTDDN GKDRLTQMKR ILKQRGNKAR GEEELQENQE LIREKSNGKR
Length:100
Mass (Da):11,267
Last modified:December 15, 1998 - v2
Checksum:i3B0050FDFF2B9DFB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti85L → V1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043141 mRNA. Translation: AAC15669.1.
PIRiA05168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043141 mRNA. Translation: AAC15669.1.
PIRiA05168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AD7NMR-A81-97[»]
1AWYNMR-A81-97[»]
1ONUNMR-A81-97[»]
2DPQX-ray1.25A81-97[»]
ProteinModelPortaliP07231.
SMRiP07231.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ConoServeri1373. Conantokin-G precursor.
1353. Conantokin-G precursor (variant).

Miscellaneous databases

EvolutionaryTraceiP07231.

Family and domain databases

InterProiView protein in InterPro
IPR005918. Conantokin_CS.
PROSITEiView protein in PROSITE
PS60025. CONANTOKIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCKG_CONGE
AccessioniPrimary (citable) accession number: P07231
Secondary accession number(s): O61475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Pharmaceutical

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.