P07214 (SPRC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SPARC

Alternative name(s):
Basement-membrane protein 40
Short name=BM-40
Osteonectin
Short name=ON
Secreted protein acidic and rich in cysteine

Gene names

Name:

Sparc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Note: In or around the basement membrane. Ref.6
Developmental stage	Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Post-translational modification	N-glycosylated. Ref.6
Sequence similarities	Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Signal
Ligand	Calcium Copper Metal-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	bone development Inferred from mutant phenotype PubMed 22876197. Source: MGI cellular response to growth factor stimulus Inferred from direct assay PubMed 7848824. Source: MGI heart development Inferred from electronic annotation. Source: Compara inner ear development Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara ossification Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from genetic interaction PubMed 17299058. Source: MGI response to L-ascorbic acid Inferred from electronic annotation. Source: Compara response to cAMP Inferred from electronic annotation. Source: Compara response to cadmium ion Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to gravity Inferred from electronic annotation. Source: Compara response to lead ion Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to peptide hormone stimulus Inferred from electronic annotation. Source: Compara signal transduction Inferred from electronic annotation. Source: InterPro wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: Compara extracellular matrix Inferred from direct assay PubMed 8906420. Source: MGI extracellular space Inferred from electronic annotation. Source: InterPro membrane-bounded vesicle Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro extracellular matrix binding Inferred from direct assay PubMed 18757743. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.5
	Chain	18 – 302	285	SPARC		PRO_0000020305
Regions
	Domain	70 – 92	23	Follistatin-like
	Domain	88 – 150	63	Kazal-like
	Domain	260 – 295	36	EF-hand
	Calcium binding	273 – 284	12	By similarity
	Compositional bias	23 – 68	46	Asp/Glu-rich (acidic; binds calcium)
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Probable
	Disulfide bond	71 ↔ 82		By similarity
	Disulfide bond	76 ↔ 92		By similarity
	Disulfide bond	94 ↔ 129		By similarity
	Disulfide bond	100 ↔ 122		By similarity
	Disulfide bond	111 ↔ 148		By similarity
	Disulfide bond	154 ↔ 264
	Disulfide bond	272 ↔ 288

Sequences

	Sequence		Length	Mass (Da)
	P07214 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: A1FF717CA32F0F5C	FASTA	302	34,450
	10 20 30 40 50 60 MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE MGEFEDGAEE 70 80 90 100 110 120 TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC PAPIGEFEKV CSNDNKTFDS 130 140 150 160 170 180 SCHFFATKCT LEGTKKGHKL HLDYIGPCKY IAPCLDSELT EFPLRMRDWL KNVLVTLYER 190 200 210 220 230 240 DEGNNLLTEK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH 250 260 270 280 290 300 PIDGYLSHTE LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL VI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)." Lankat-Buttgereit B., Mann K., Deutzmann R., Timpl R., Krieg T. FEBS Lett. 236:352-356(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE. Tissue: Placenta.
[2]	"Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000." Mason I.J., Taylor A., Williams J.G., Sage H., Hogan B.L.M. EMBO J. 5:1465-1472(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region." McVey J.H., Nomura S., Kelly P., Mason I.J., Hogan B.L.M. J. Biol. Chem. 263:11111-11116(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC." Mann K., Deutzmann R., Paulsson M., Timpl R. FEBS Lett. 218:167-172(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-37 AND 215-234.
[6]	"Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues." Engel J., Taylor W., Paulsson M., Sage H., Hogan B.L.M. Biochemistry 26:6958-6965(1987) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, CALCIUM-BINDING, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04017 mRNA. Translation: CAA27642.1. M20692 , M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1. BC004638 mRNA. Translation: AAH04638.1.
IPI	IPI00126343.
PIR	GEMSN. A29227.
RefSeq	NP_033268.1. NM_009242.4.
UniGene	Mm.291442.
3D structure databases
DisProt	DP00052.
ProteinModelPortal	P07214.
SMR	P07214. Positions 70-302.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07214. 2 interactions.
PTM databases
PhosphoSite	P07214.
Proteomic databases
PaxDb	P07214.
PRIDE	P07214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
GeneID	20692.
KEGG	mmu:20692.
Organism-specific databases
CTD	6678.
MGI	MGI:98373. Sparc.
Phylogenomic databases
eggNOG	NOG147672.
HOGENOM	HOG000088654.
HOVERGEN	HBG002746.
InParanoid	P07214.
OMA	CELDESN.
OrthoDB	EOG4T7842.
Gene expression databases
ArrayExpress	P07214.
Bgee	P07214.
CleanEx	MM_SPARC.
Genevestigator	P07214.
GermOnline	ENSMUSG00000018593. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR003645. Fol_N. IPR015369. Follistatin/Osteonectin_EGF. IPR002350. Kazal_dom. IPR001999. Osteonectin_CS. IPR019577. SPARC/Testican_Ca-bd-dom. [Graphical view]
Pfam	PF09289. FOLN. 1 hit. PF00050. Kazal_1. 1 hit. PF10591. SPARC_Ca_bdg. 1 hit. [Graphical view]
SMART	SM00274. FOLN. 1 hit. SM00280. KAZAL. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. False negative. PS51465. KAZAL_2. 1 hit. PS00612. OSTEONECTIN_1. 1 hit. PS00613. OSTEONECTIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SPARC. mouse.
NextBio	299233.
SOURCE	Search...

Entry information

Entry name

SPRC_MOUSE

Accession

Primary (citable) accession number: P07214

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program