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Protein

SPARC

Gene

Sparc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi273 – 284By similarityAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: CAFA
  • collagen binding Source: MGI
  • extracellular matrix binding Source: MGI

GO - Biological processi

Keywordsi

LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-3000178. ECM proteoglycans.
R-MMU-3000497. Scavenging by Class H Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98373. Sparc.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • cell surface Source: MGI
  • extracellular space Source: BHF-UCL
  • nuclear matrix Source: MGI
  • platelet alpha granule Source: MGI
  • platelet alpha granule membrane Source: MGI
  • proteinaceous extracellular matrix Source: MGI

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002030518 – 302SPARCAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 82PROSITE-ProRule annotation
Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
Disulfide bondi94 ↔ 129PROSITE-ProRule annotation
Disulfide bondi100 ↔ 122PROSITE-ProRule annotation
Disulfide bondi111 ↔ 148PROSITE-ProRule annotation
Glycosylationi115N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi154 ↔ 264
Disulfide bondi272 ↔ 288

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP07214.
PeptideAtlasiP07214.
PRIDEiP07214.

PTM databases

PhosphoSitePlusiP07214.

Expressioni

Developmental stagei

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

BgeeiENSMUSG00000018593.
CleanExiMM_SPARC.
ExpressionAtlasiP07214. baseline and differential.
GenevisibleiP07214. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203423. 2 interactors.
IntActiP07214. 3 interactors.
MINTiMINT-4996368.
STRINGi10090.ENSMUSP00000018737.

Structurei

3D structure databases

DisProtiDP00052.
ProteinModelPortaliP07214.
SMRiP07214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 92Follistatin-likeAdd BLAST23
Domaini88 – 150Kazal-likePROSITE-ProRule annotationAdd BLAST63
Domaini260 – 295EF-handAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 68Asp/Glu-rich (acidic; binds calcium)Add BLAST46

Sequence similaritiesi

Belongs to the SPARC family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP07214.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.
PhylomeDBiP07214.
TreeFamiTF319356.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
PfamiView protein in Pfam
PF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
SMARTiView protein in SMART
SM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
SUPFAMiSSF100895. SSF100895. 1 hit.
SSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE
60 70 80 90 100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC
110 120 130 140 150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY
160 170 180 190 200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE
210 220 230 240 250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE
260 270 280 290 300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL

VI
Length:302
Mass (Da):34,450
Last modified:April 1, 1988 - v1
Checksum:iA1FF717CA32F0F5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA. Translation: CAA27642.1.
M20692
, M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
BC004638 mRNA. Translation: AAH04638.1.
CCDSiCCDS24712.1.
PIRiA29227. GEMSN.
RefSeqiNP_033268.1. NM_009242.5.
UniGeneiMm.291442.

Genome annotation databases

EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
ENSMUST00000214685; ENSMUSP00000151000; ENSMUSG00000018593.
GeneIDi20692.
KEGGimmu:20692.
UCSCiuc007izh.3. mouse.

Similar proteinsi

Entry informationi

Entry nameiSPRC_MOUSE
AccessioniPrimary (citable) accession number: P07214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: August 30, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families