Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

SPARC

Gene

Sparc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi273 – 28412By similarityAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • collagen binding Source: MGI
  • extracellular matrix binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-3000178. ECM proteoglycans.
R-MMU-3000497. Scavenging by Class H Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98373. Sparc.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 302285SPARCPRO_0000020305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi71 ↔ 82PROSITE-ProRule annotation
Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
Disulfide bondi94 ↔ 129PROSITE-ProRule annotation
Disulfide bondi100 ↔ 122PROSITE-ProRule annotation
Disulfide bondi111 ↔ 148PROSITE-ProRule annotation
Glycosylationi115 – 1151N-linked (GlcNAc...)1 Publication
Disulfide bondi154 ↔ 264
Disulfide bondi272 ↔ 288

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP07214.
PeptideAtlasiP07214.
PRIDEiP07214.

PTM databases

PhosphoSiteiP07214.

Expressioni

Developmental stagei

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

BgeeiENSMUSG00000018593.
CleanExiMM_SPARC.
ExpressionAtlasiP07214. baseline and differential.
GenevisibleiP07214. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203423. 2 interactions.
IntActiP07214. 3 interactions.
MINTiMINT-4996368.
STRINGi10090.ENSMUSP00000018737.

Structurei

3D structure databases

DisProtiDP00052.
ProteinModelPortaliP07214.
SMRiP07214. Positions 70-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 9223Follistatin-likeAdd
BLAST
Domaini88 – 15063Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini260 – 29536EF-handAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 6846Asp/Glu-rich (acidic; binds calcium)Add
BLAST

Sequence similaritiesi

Belongs to the SPARC family.Curated
Contains 1 EF-hand domain.Curated
Contains 1 follistatin-like domain.Curated
Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP07214.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.
PhylomeDBiP07214.
TreeFamiTF319356.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE
60 70 80 90 100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC
110 120 130 140 150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY
160 170 180 190 200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE
210 220 230 240 250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE
260 270 280 290 300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL

VI
Length:302
Mass (Da):34,450
Last modified:April 1, 1988 - v1
Checksum:iA1FF717CA32F0F5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA. Translation: CAA27642.1.
M20692
, M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
BC004638 mRNA. Translation: AAH04638.1.
CCDSiCCDS24712.1.
PIRiA29227. GEMSN.
RefSeqiNP_033268.1. NM_009242.5.
UniGeneiMm.291442.

Genome annotation databases

EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
GeneIDi20692.
KEGGimmu:20692.
UCSCiuc007izh.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA. Translation: CAA27642.1.
M20692
, M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
BC004638 mRNA. Translation: AAH04638.1.
CCDSiCCDS24712.1.
PIRiA29227. GEMSN.
RefSeqiNP_033268.1. NM_009242.5.
UniGeneiMm.291442.

3D structure databases

DisProtiDP00052.
ProteinModelPortaliP07214.
SMRiP07214. Positions 70-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203423. 2 interactions.
IntActiP07214. 3 interactions.
MINTiMINT-4996368.
STRINGi10090.ENSMUSP00000018737.

PTM databases

PhosphoSiteiP07214.

Proteomic databases

PaxDbiP07214.
PeptideAtlasiP07214.
PRIDEiP07214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
GeneIDi20692.
KEGGimmu:20692.
UCSCiuc007izh.3. mouse.

Organism-specific databases

CTDi6678.
MGIiMGI:98373. Sparc.

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP07214.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.
PhylomeDBiP07214.
TreeFamiTF319356.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-3000178. ECM proteoglycans.
R-MMU-3000497. Scavenging by Class H Receptors.

Miscellaneous databases

ChiTaRSiSparc. mouse.
PROiP07214.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018593.
CleanExiMM_SPARC.
ExpressionAtlasiP07214. baseline and differential.
GenevisibleiP07214. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPRC_MOUSE
AccessioniPrimary (citable) accession number: P07214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 7, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.