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P07214

- SPRC_MOUSE

UniProt

P07214 - SPRC_MOUSE

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Protein

SPARC

Gene

Sparc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi273 – 28412By similarityAdd
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: Ensembl
  2. extracellular matrix binding Source: MGI

GO - Biological processi

  1. bone development Source: MGI
  2. cellular response to growth factor stimulus Source: MGI
  3. heart development Source: Ensembl
  4. inner ear development Source: Ensembl
  5. lung development Source: Ensembl
  6. ossification Source: Ensembl
  7. regulation of cell proliferation Source: MGI
  8. response to cadmium ion Source: Ensembl
  9. response to calcium ion Source: Ensembl
  10. response to cAMP Source: Ensembl
  11. response to cytokine Source: Ensembl
  12. response to ethanol Source: Ensembl
  13. response to glucocorticoid Source: Ensembl
  14. response to gravity Source: Ensembl
  15. response to L-ascorbic acid Source: Ensembl
  16. response to lead ion Source: Ensembl
  17. response to lipopolysaccharide Source: Ensembl
  18. response to peptide hormone Source: Ensembl
  19. signal transduction Source: InterPro
  20. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Calcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196594. Scavenging by Class H Receptors.
REACT_196606. ECM proteoglycans.

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:98373. Sparc.

Subcellular locationi

Secretedextracellular spaceextracellular matrixbasement membrane 1 Publication
Note: In or around the basement membrane.

GO - Cellular componenti

  1. basement membrane Source: UniProtKB-KW
  2. cytoplasm Source: Ensembl
  3. extracellular matrix Source: MGI
  4. extracellular space Source: Ensembl
  5. membrane-bounded vesicle Source: Ensembl
  6. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 302285SPARCPRO_0000020305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi71 ↔ 82PROSITE-ProRule annotation
Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
Disulfide bondi94 ↔ 129PROSITE-ProRule annotation
Disulfide bondi100 ↔ 122PROSITE-ProRule annotation
Disulfide bondi111 ↔ 148PROSITE-ProRule annotation
Glycosylationi115 – 1151N-linked (GlcNAc...)1 Publication
Disulfide bondi154 ↔ 264
Disulfide bondi272 ↔ 288

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP07214.
PaxDbiP07214.
PRIDEiP07214.

PTM databases

PhosphoSiteiP07214.

Expressioni

Developmental stagei

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Gene expression databases

BgeeiP07214.
CleanExiMM_SPARC.
ExpressionAtlasiP07214. baseline and differential.
GenevestigatoriP07214.

Interactioni

Protein-protein interaction databases

BioGridi203423. 1 interaction.
IntActiP07214. 3 interactions.
MINTiMINT-4996368.

Structurei

3D structure databases

DisProtiDP00052.
ProteinModelPortaliP07214.
SMRiP07214. Positions 70-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 9223Follistatin-likeAdd
BLAST
Domaini88 – 15063Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini260 – 29536EF-handAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 6846Asp/Glu-rich (acidic; binds calcium)Add
BLAST

Sequence similaritiesi

Belongs to the SPARC family.Curated
Contains 1 EF-hand domain.Curated
Contains 1 follistatin-like domain.Curated
Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG147672.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP07214.
OMAiCFGIKEK.
OrthoDBiEOG738059.
PhylomeDBiP07214.
TreeFamiTF319356.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07214-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE
60 70 80 90 100
MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC
110 120 130 140 150
PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY
160 170 180 190 200
IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE
210 220 230 240 250
NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE
260 270 280 290 300
LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL

VI
Length:302
Mass (Da):34,450
Last modified:April 1, 1988 - v1
Checksum:iA1FF717CA32F0F5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA. Translation: CAA27642.1.
M20692
, M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
BC004638 mRNA. Translation: AAH04638.1.
CCDSiCCDS24712.1.
PIRiA29227. GEMSN.
RefSeqiNP_033268.1. NM_009242.5.
XP_006532729.1. XM_006532666.1.
UniGeneiMm.291442.

Genome annotation databases

EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
GeneIDi20692.
KEGGimmu:20692.
UCSCiuc007izh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04017 mRNA. Translation: CAA27642.1 .
M20692
, M20684 , M20685 , M20686 , M20687 , M20688 , M20689 , M20690 , M20691 Genomic DNA. Translation: AAA40125.1 .
BC004638 mRNA. Translation: AAH04638.1 .
CCDSi CCDS24712.1.
PIRi A29227. GEMSN.
RefSeqi NP_033268.1. NM_009242.5.
XP_006532729.1. XM_006532666.1.
UniGenei Mm.291442.

3D structure databases

DisProti DP00052.
ProteinModelPortali P07214.
SMRi P07214. Positions 70-302.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203423. 1 interaction.
IntActi P07214. 3 interactions.
MINTi MINT-4996368.

PTM databases

PhosphoSitei P07214.

Proteomic databases

MaxQBi P07214.
PaxDbi P07214.
PRIDEi P07214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018737 ; ENSMUSP00000018737 ; ENSMUSG00000018593 .
GeneIDi 20692.
KEGGi mmu:20692.
UCSCi uc007izh.2. mouse.

Organism-specific databases

CTDi 6678.
MGIi MGI:98373. Sparc.

Phylogenomic databases

eggNOGi NOG147672.
HOGENOMi HOG000088654.
HOVERGENi HBG002746.
InParanoidi P07214.
OMAi CFGIKEK.
OrthoDBi EOG738059.
PhylomeDBi P07214.
TreeFami TF319356.

Enzyme and pathway databases

Reactomei REACT_196594. Scavenging by Class H Receptors.
REACT_196606. ECM proteoglycans.

Miscellaneous databases

ChiTaRSi Sparc. mouse.
NextBioi 299233.
PROi P07214.
SOURCEi Search...

Gene expression databases

Bgeei P07214.
CleanExi MM_SPARC.
ExpressionAtlasi P07214. baseline and differential.
Genevestigatori P07214.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view ]
Pfami PF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view ]
SMARTi SM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)."
    Lankat-Buttgereit B., Mann K., Deutzmann R., Timpl R., Krieg T.
    FEBS Lett. 236:352-356(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
    Tissue: Placenta.
  2. "Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000."
    Mason I.J., Taylor A., Williams J.G., Sage H., Hogan B.L.M.
    EMBO J. 5:1465-1472(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region."
    McVey J.H., Nomura S., Kelly P., Mason I.J., Hogan B.L.M.
    J. Biol. Chem. 263:11111-11116(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC."
    Mann K., Deutzmann R., Paulsson M., Timpl R.
    FEBS Lett. 218:167-172(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-37 AND 215-234.
  6. "Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues."
    Engel J., Taylor W., Paulsson M., Sage H., Hogan B.L.M.
    Biochemistry 26:6958-6965(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION, CALCIUM-BINDING, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSPRC_MOUSE
AccessioniPrimary (citable) accession number: P07214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3