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P07214 (SPRC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name=BM-40
Osteonectin
Short name=ON
Secreted protein acidic and rich in cysteine
Gene names
Name:Sparc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane. Note: In or around the basement membrane. Ref.6

Developmental stage

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

Post-translational modification

N-glycosylated. Ref.6

Sequence similarities

Belongs to the SPARC family.

Contains 1 EF-hand domain.

Contains 1 follistatin-like domain.

Contains 1 Kazal-like domain.

Ontologies

Keywords
   Cellular componentBasement membrane
Extracellular matrix
Secreted
   DomainSignal
   LigandCalcium
Copper
Metal-binding
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processbone development

Inferred from mutant phenotype PubMed 22876197. Source: MGI

cellular response to growth factor stimulus

Inferred from direct assay PubMed 7848824. Source: MGI

heart development

Inferred from electronic annotation. Source: Ensembl

inner ear development

Inferred from electronic annotation. Source: Ensembl

lung development

Inferred from electronic annotation. Source: Ensembl

ossification

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from genetic interaction PubMed 17299058. Source: MGI

response to L-ascorbic acid

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to cadmium ion

Inferred from electronic annotation. Source: Ensembl

response to calcium ion

Inferred from electronic annotation. Source: Ensembl

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to gravity

Inferred from electronic annotation. Source: Ensembl

response to lead ion

Inferred from electronic annotation. Source: Ensembl

response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from electronic annotation. Source: InterPro

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasement membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: Ensembl

extracellular matrix

Inferred from direct assay PubMed 8906420. Source: MGI

extracellular space

Inferred from electronic annotation. Source: Ensembl

membrane-bounded vesicle

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: Ensembl

extracellular matrix binding

Inferred from direct assay PubMed 18757743. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.5
Chain18 – 302285SPARC
PRO_0000020305

Regions

Domain70 – 9223Follistatin-like
Domain88 – 15063Kazal-like
Domain260 – 29536EF-hand
Calcium binding273 – 28412 By similarity
Compositional bias23 – 6846Asp/Glu-rich (acidic; binds calcium)

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) Probable
Disulfide bond71 ↔ 82 By similarity
Disulfide bond76 ↔ 92 By similarity
Disulfide bond94 ↔ 129 By similarity
Disulfide bond100 ↔ 122 By similarity
Disulfide bond111 ↔ 148 By similarity
Disulfide bond154 ↔ 264
Disulfide bond272 ↔ 288

Sequences

Sequence LengthMass (Da)Tools
P07214 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: A1FF717CA32F0F5C

FASTA30234,450
        10         20         30         40         50         60 
MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE MGEFEDGAEE 

        70         80         90        100        110        120 
TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC PAPIGEFEKV CSNDNKTFDS 

       130        140        150        160        170        180 
SCHFFATKCT LEGTKKGHKL HLDYIGPCKY IAPCLDSELT EFPLRMRDWL KNVLVTLYER 

       190        200        210        220        230        240 
DEGNNLLTEK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH 

       250        260        270        280        290        300 
PIDGYLSHTE LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL 


VI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)."
Lankat-Buttgereit B., Mann K., Deutzmann R., Timpl R., Krieg T.
FEBS Lett. 236:352-356(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
Tissue: Placenta.
[2]"Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000."
Mason I.J., Taylor A., Williams J.G., Sage H., Hogan B.L.M.
EMBO J. 5:1465-1472(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region."
McVey J.H., Nomura S., Kelly P., Mason I.J., Hogan B.L.M.
J. Biol. Chem. 263:11111-11116(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC."
Mann K., Deutzmann R., Paulsson M., Timpl R.
FEBS Lett. 218:167-172(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-37 AND 215-234.
[6]"Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues."
Engel J., Taylor W., Paulsson M., Sage H., Hogan B.L.M.
Biochemistry 26:6958-6965(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION, CALCIUM-BINDING, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04017 mRNA. Translation: CAA27642.1.
M20692 expand/collapse EMBL AC list , M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
BC004638 mRNA. Translation: AAH04638.1.
PIRGEMSN. A29227.
RefSeqNP_033268.1. NM_009242.4.
XP_006532729.1. XM_006532666.1.
UniGeneMm.291442.

3D structure databases

DisProtDP00052.
ProteinModelPortalP07214.
SMRP07214. Positions 70-302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203423. 1 interaction.
IntActP07214. 3 interactions.
MINTMINT-4996368.

PTM databases

PhosphoSiteP07214.

Proteomic databases

PaxDbP07214.
PRIDEP07214.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
GeneID20692.
KEGGmmu:20692.
UCSCuc007izh.2. mouse.

Organism-specific databases

CTD6678.
MGIMGI:98373. Sparc.

Phylogenomic databases

eggNOGNOG147672.
HOGENOMHOG000088654.
HOVERGENHBG002746.
InParanoidP07214.
OMACFGIKEK.
OrthoDBEOG738059.
PhylomeDBP07214.
TreeFamTF319356.

Gene expression databases

ArrayExpressP07214.
BgeeP07214.
CleanExMM_SPARC.
GenevestigatorP07214.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSPARC. mouse.
NextBio299233.
PROP07214.
SOURCESearch...

Entry information

Entry nameSPRC_MOUSE
AccessionPrimary (citable) accession number: P07214
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot