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P07214

- SPRC_MOUSE

UniProt

P07214 - SPRC_MOUSE

Protein

SPARC

Gene

Sparc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi273 – 28412By similarityAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: Ensembl
    2. extracellular matrix binding Source: MGI

    GO - Biological processi

    1. bone development Source: MGI
    2. cellular response to growth factor stimulus Source: MGI
    3. heart development Source: Ensembl
    4. inner ear development Source: Ensembl
    5. lung development Source: Ensembl
    6. ossification Source: Ensembl
    7. regulation of cell proliferation Source: MGI
    8. response to cadmium ion Source: Ensembl
    9. response to calcium ion Source: Ensembl
    10. response to cAMP Source: Ensembl
    11. response to cytokine Source: Ensembl
    12. response to ethanol Source: Ensembl
    13. response to glucocorticoid Source: Ensembl
    14. response to gravity Source: Ensembl
    15. response to L-ascorbic acid Source: Ensembl
    16. response to lead ion Source: Ensembl
    17. response to lipopolysaccharide Source: Ensembl
    18. response to peptide hormone Source: Ensembl
    19. signal transduction Source: InterPro
    20. wound healing Source: Ensembl

    Keywords - Ligandi

    Calcium, Copper, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196594. Scavenging by Class H Receptors.
    REACT_196606. ECM proteoglycans.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SPARC
    Alternative name(s):
    Basement-membrane protein 40
    Short name:
    BM-40
    Osteonectin
    Short name:
    ON
    Secreted protein acidic and rich in cysteine
    Gene namesi
    Name:Sparc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:98373. Sparc.

    Subcellular locationi

    Secretedextracellular spaceextracellular matrixbasement membrane 1 Publication
    Note: In or around the basement membrane.

    GO - Cellular componenti

    1. basement membrane Source: UniProtKB-SubCell
    2. cytoplasm Source: Ensembl
    3. extracellular matrix Source: MGI
    4. extracellular space Source: Ensembl
    5. membrane-bounded vesicle Source: Ensembl
    6. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Basement membrane, Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 302285SPARCPRO_0000020305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi71 ↔ 82PROSITE-ProRule annotation
    Disulfide bondi76 ↔ 92PROSITE-ProRule annotation
    Disulfide bondi94 ↔ 129PROSITE-ProRule annotation
    Disulfide bondi100 ↔ 122PROSITE-ProRule annotation
    Disulfide bondi111 ↔ 148PROSITE-ProRule annotation
    Glycosylationi115 – 1151N-linked (GlcNAc...)1 Publication
    Disulfide bondi154 ↔ 264
    Disulfide bondi272 ↔ 288

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP07214.
    PaxDbiP07214.
    PRIDEiP07214.

    PTM databases

    PhosphoSiteiP07214.

    Expressioni

    Developmental stagei

    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.

    Gene expression databases

    ArrayExpressiP07214.
    BgeeiP07214.
    CleanExiMM_SPARC.
    GenevestigatoriP07214.

    Interactioni

    Protein-protein interaction databases

    BioGridi203423. 1 interaction.
    IntActiP07214. 3 interactions.
    MINTiMINT-4996368.

    Structurei

    3D structure databases

    DisProtiDP00052.
    ProteinModelPortaliP07214.
    SMRiP07214. Positions 70-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini70 – 9223Follistatin-likeAdd
    BLAST
    Domaini88 – 15063Kazal-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini260 – 29536EF-handAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi23 – 6846Asp/Glu-rich (acidic; binds calcium)Add
    BLAST

    Sequence similaritiesi

    Belongs to the SPARC family.Curated
    Contains 1 EF-hand domain.Curated
    Contains 1 follistatin-like domain.Curated
    Contains 1 Kazal-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG147672.
    HOGENOMiHOG000088654.
    HOVERGENiHBG002746.
    InParanoidiP07214.
    OMAiCFGIKEK.
    OrthoDBiEOG738059.
    PhylomeDBiP07214.
    TreeFamiTF319356.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR003645. Fol_N.
    IPR015369. Follistatin/Osteonectin_EGF.
    IPR002350. Kazal_dom.
    IPR001999. Osteonectin_CS.
    IPR019577. SPARC/Testican_Ca-bd-dom.
    [Graphical view]
    PfamiPF09289. FOLN. 1 hit.
    PF00050. Kazal_1. 1 hit.
    PF10591. SPARC_Ca_bdg. 1 hit.
    [Graphical view]
    SMARTiSM00274. FOLN. 1 hit.
    SM00280. KAZAL. 1 hit.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    PS00612. OSTEONECTIN_1. 1 hit.
    PS00613. OSTEONECTIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07214-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAWIFFLLC LAGRALAAPQ QTEVAEEIVE EETVVEETGV PVGANPVQVE    50
    MGEFEDGAEE TVEEVVADNP CQNHHCKHGK VCELDESNTP MCVCQDPTSC 100
    PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY 150
    IAPCLDSELT EFPLRMRDWL KNVLVTLYER DEGNNLLTEK QKLRVKKIHE 200
    NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQH PIDGYLSHTE 250
    LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL 300
    VI 302
    Length:302
    Mass (Da):34,450
    Last modified:April 1, 1988 - v1
    Checksum:iA1FF717CA32F0F5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04017 mRNA. Translation: CAA27642.1.
    M20692
    , M20684, M20685, M20686, M20687, M20688, M20689, M20690, M20691 Genomic DNA. Translation: AAA40125.1.
    BC004638 mRNA. Translation: AAH04638.1.
    CCDSiCCDS24712.1.
    PIRiA29227. GEMSN.
    RefSeqiNP_033268.1. NM_009242.5.
    XP_006532729.1. XM_006532666.1.
    UniGeneiMm.291442.

    Genome annotation databases

    EnsembliENSMUST00000018737; ENSMUSP00000018737; ENSMUSG00000018593.
    GeneIDi20692.
    KEGGimmu:20692.
    UCSCiuc007izh.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04017 mRNA. Translation: CAA27642.1 .
    M20692
    , M20684 , M20685 , M20686 , M20687 , M20688 , M20689 , M20690 , M20691 Genomic DNA. Translation: AAA40125.1 .
    BC004638 mRNA. Translation: AAH04638.1 .
    CCDSi CCDS24712.1.
    PIRi A29227. GEMSN.
    RefSeqi NP_033268.1. NM_009242.5.
    XP_006532729.1. XM_006532666.1.
    UniGenei Mm.291442.

    3D structure databases

    DisProti DP00052.
    ProteinModelPortali P07214.
    SMRi P07214. Positions 70-302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203423. 1 interaction.
    IntActi P07214. 3 interactions.
    MINTi MINT-4996368.

    PTM databases

    PhosphoSitei P07214.

    Proteomic databases

    MaxQBi P07214.
    PaxDbi P07214.
    PRIDEi P07214.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018737 ; ENSMUSP00000018737 ; ENSMUSG00000018593 .
    GeneIDi 20692.
    KEGGi mmu:20692.
    UCSCi uc007izh.2. mouse.

    Organism-specific databases

    CTDi 6678.
    MGIi MGI:98373. Sparc.

    Phylogenomic databases

    eggNOGi NOG147672.
    HOGENOMi HOG000088654.
    HOVERGENi HBG002746.
    InParanoidi P07214.
    OMAi CFGIKEK.
    OrthoDBi EOG738059.
    PhylomeDBi P07214.
    TreeFami TF319356.

    Enzyme and pathway databases

    Reactomei REACT_196594. Scavenging by Class H Receptors.
    REACT_196606. ECM proteoglycans.

    Miscellaneous databases

    ChiTaRSi SPARC. mouse.
    NextBioi 299233.
    PROi P07214.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P07214.
    Bgeei P07214.
    CleanExi MM_SPARC.
    Genevestigatori P07214.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR003645. Fol_N.
    IPR015369. Follistatin/Osteonectin_EGF.
    IPR002350. Kazal_dom.
    IPR001999. Osteonectin_CS.
    IPR019577. SPARC/Testican_Ca-bd-dom.
    [Graphical view ]
    Pfami PF09289. FOLN. 1 hit.
    PF00050. Kazal_1. 1 hit.
    PF10591. SPARC_Ca_bdg. 1 hit.
    [Graphical view ]
    SMARTi SM00274. FOLN. 1 hit.
    SM00280. KAZAL. 1 hit.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS51465. KAZAL_2. 1 hit.
    PS00612. OSTEONECTIN_1. 1 hit.
    PS00613. OSTEONECTIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)."
      Lankat-Buttgereit B., Mann K., Deutzmann R., Timpl R., Krieg T.
      FEBS Lett. 236:352-356(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE.
      Tissue: Placenta.
    2. "Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000."
      Mason I.J., Taylor A., Williams J.G., Sage H., Hogan B.L.M.
      EMBO J. 5:1465-1472(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region."
      McVey J.H., Nomura S., Kelly P., Mason I.J., Hogan B.L.M.
      J. Biol. Chem. 263:11111-11116(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Solubilization of protein BM-40 from a basement membrane tumor with chelating agents and evidence for its identity with osteonectin and SPARC."
      Mann K., Deutzmann R., Paulsson M., Timpl R.
      FEBS Lett. 218:167-172(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-37 AND 215-234.
    6. "Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues."
      Engel J., Taylor W., Paulsson M., Sage H., Hogan B.L.M.
      Biochemistry 26:6958-6965(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, CALCIUM-BINDING, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSPRC_MOUSE
    AccessioniPrimary (citable) accession number: P07214
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3