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Protein

Mitochondrial import receptor subunit TOM70

Gene

TOM70

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.1 Publication

GO - Molecular functioni

  • mitochondrion targeting sequence binding Source: SGD
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro

GO - Biological processi

  • protein import into mitochondrial inner membrane Source: SGD
  • protein import into mitochondrial matrix Source: SGD
  • protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33142-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM70
Alternative name(s):
70 kDa mitochondrial outer membrane protein
Translocase of outer membrane 70 kDa subunit
Gene namesi
Name:TOM70
Synonyms:MAS70, OMP1
Ordered Locus Names:YNL121C
ORF Names:N1905
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL121C.
SGDiS000005065. TOM70.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10Mitochondrial intermembraneSequence analysis10
Transmembranei11 – 30HelicalSequence analysisAdd BLAST20
Topological domaini31 – 617CytoplasmicSequence analysisAdd BLAST587

GO - Cellular componenti

  • integral component of mitochondrial outer membrane Source: SGD
  • mitochondrial outer membrane Source: Reactome
  • mitochondrial outer membrane translocase complex Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063401 – 617Mitochondrial import receptor subunit TOM70Add BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07213.
PRIDEiP07213.

PTM databases

iPTMnetiP07213.

Interactioni

Subunit structurei

Forms part of the TOM (translocase of outer membrane) complex that consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex interacts with TOM22. Interacts with TOM37.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BAXQ078122EBI-12551,EBI-516580From a different organism.

Protein-protein interaction databases

BioGridi35706. 161 interactors.
DIPiDIP-2301N.
IntActiP07213. 12 interactors.
MINTiMINT-508943.

Structurei

Secondary structure

1617
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi95 – 111Combined sources17
Helixi115 – 128Combined sources14
Helixi132 – 145Combined sources14
Helixi148 – 161Combined sources14
Helixi166 – 178Combined sources13
Helixi182 – 194Combined sources13
Beta strandi195 – 197Combined sources3
Helixi200 – 202Combined sources3
Helixi204 – 219Combined sources16
Helixi254 – 261Combined sources8
Helixi278 – 290Combined sources13
Helixi297 – 315Combined sources19
Turni316 – 318Combined sources3
Helixi323 – 342Combined sources20
Helixi346 – 359Combined sources14
Helixi363 – 374Combined sources12
Helixi382 – 384Combined sources3
Helixi385 – 390Combined sources6
Helixi398 – 409Combined sources12
Helixi415 – 425Combined sources11
Helixi432 – 440Combined sources9
Turni441 – 445Combined sources5
Helixi447 – 460Combined sources14
Helixi466 – 477Combined sources12
Helixi481 – 496Combined sources16
Beta strandi498 – 500Combined sources3
Helixi506 – 516Combined sources11
Helixi523 – 537Combined sources15
Helixi542 – 554Combined sources13
Helixi558 – 571Combined sources14
Helixi575 – 594Combined sources20
Helixi598 – 605Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GW1X-ray3.00A/B94-607[»]
ProteinModelPortaliP07213.
SMRiP07213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati99 – 132TPR 1Add BLAST34
Repeati134 – 165TPR 2Add BLAST32
Repeati281 – 315TPR 3Add BLAST35
Repeati363 – 396TPR 4Add BLAST34
Repeati397 – 430TPR 5Add BLAST34
Repeati432 – 464TPR 6Add BLAST33
Repeati465 – 498TPR 7Add BLAST34
Repeati505 – 541TPR 8Add BLAST37
Repeati542 – 575TPR 9Add BLAST34

Sequence similaritiesi

Belongs to the Tom70 family.Curated
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133798.
InParanoidiP07213.
KOiK17768.
OMAiCITYKNG.
OrthoDBiEOG092C1Q9V.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005687. Tom70.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
TIGRFAMsiTIGR00990. 3a0801s09. 1 hit.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFITRNKT AILATVAATG TAIGAYYYYN QLQQQQQRGK KNTINKDEKK
60 70 80 90 100
DTKDSQKETE GAKKSTAPSN PPIYPVSSNG EPDFSNKANF TAEEKDKYAL
110 120 130 140 150
ALKDKGNQFF RNKKYDDAIK YYNWALELKE DPVFYSNLSA CYVSVGDLKK
160 170 180 190 200
VVEMSTKALE LKPDYSKVLL RRASANEGLG KFADAMFDLS VLSLNGDFND
210 220 230 240 250
ASIEPMLERN LNKQAMSKLK EKFGDIDTAT ATPTELSTQP AKERKDKQEN
260 270 280 290 300
LPSVTSMASF FGIFKPELTF ANYDESNEAD KELMNGLSNL YKRSPESYDK
310 320 330 340 350
ADESFTKAAR LFEEQLDKNN EDEKLKEKLA ISLEHTGIFK FLKNDPLGAH
360 370 380 390 400
EDIKKAIELF PRVNSYIYMA LIMADRNDST EYYNYFDKAL KLDSNNSSVY
410 420 430 440 450
YHRGQMNFIL QNYDQAGKDF DKAKELDPEN IFPYIQLACL AYRENKFDDC
460 470 480 490 500
ETLFSEAKRK FPEAPEVPNF FAEILTDKND FDKALKQYDL AIELENKLDG
510 520 530 540 550
IYVGIAPLVG KATLLTRNPT VENFIEATNL LEKASKLDPR SEQAKIGLAQ
560 570 580 590 600
MKLQQEDIDE AITLFEESAD LARTMEEKLQ AITFAEAAKV QQRIRSDPVL
610
AKKIQETLAK LREQGLM
Length:617
Mass (Da):70,123
Last modified:October 1, 1996 - v2
Checksum:i664B8FC32CCE39A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185A → R in CAA29085 (PubMed:6365533).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti30N → S in strain: SK1, V1-09, YJM339 and YJM627. 1 Publication1
Natural varianti68P → S in strain: V1-09 and YJM627. 1 Publication1
Natural varianti277N → H in strain: YJM1129. 1 Publication1
Natural varianti359L → S in strain: SK1. 1 Publication1
Natural varianti364N → I in strain: YJM269 and YJM270. 1 Publication1
Natural varianti385Y → H in strain: YJM1129. 1 Publication1
Natural varianti429E → G in ytrain: V1-09. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05585 Genomic DNA. Translation: CAA29085.1.
EF125216 Genomic DNA. Translation: ABN58537.1.
EF125217 Genomic DNA. Translation: ABN58546.1.
EF125218 Genomic DNA. Translation: ABN58555.1.
EF125219 Genomic DNA. Translation: ABN58564.1.
EF125220 Genomic DNA. Translation: ABN58573.1.
EF125221 Genomic DNA. Translation: ABN58582.1.
EF125222 Genomic DNA. Translation: ABN58591.1.
EF125223 Genomic DNA. Translation: ABN58600.1.
EF125224 Genomic DNA. Translation: ABN58609.1.
EF125225 Genomic DNA. Translation: ABN58618.1.
EF125226 Genomic DNA. Translation: ABN58627.1.
EF125228 Genomic DNA. Translation: ABN58645.1.
Z69382 Genomic DNA. Translation: CAA93386.1.
Z71397 Genomic DNA. Translation: CAA96002.1.
BK006947 Genomic DNA. Translation: DAA10428.1.
PIRiS63062. MMBYO.
RefSeqiNP_014278.3. NM_001182959.3.

Genome annotation databases

EnsemblFungiiYNL121C; YNL121C; YNL121C.
GeneIDi855602.
KEGGisce:YNL121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05585 Genomic DNA. Translation: CAA29085.1.
EF125216 Genomic DNA. Translation: ABN58537.1.
EF125217 Genomic DNA. Translation: ABN58546.1.
EF125218 Genomic DNA. Translation: ABN58555.1.
EF125219 Genomic DNA. Translation: ABN58564.1.
EF125220 Genomic DNA. Translation: ABN58573.1.
EF125221 Genomic DNA. Translation: ABN58582.1.
EF125222 Genomic DNA. Translation: ABN58591.1.
EF125223 Genomic DNA. Translation: ABN58600.1.
EF125224 Genomic DNA. Translation: ABN58609.1.
EF125225 Genomic DNA. Translation: ABN58618.1.
EF125226 Genomic DNA. Translation: ABN58627.1.
EF125228 Genomic DNA. Translation: ABN58645.1.
Z69382 Genomic DNA. Translation: CAA93386.1.
Z71397 Genomic DNA. Translation: CAA96002.1.
BK006947 Genomic DNA. Translation: DAA10428.1.
PIRiS63062. MMBYO.
RefSeqiNP_014278.3. NM_001182959.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GW1X-ray3.00A/B94-607[»]
ProteinModelPortaliP07213.
SMRiP07213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35706. 161 interactors.
DIPiDIP-2301N.
IntActiP07213. 12 interactors.
MINTiMINT-508943.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

PTM databases

iPTMnetiP07213.

Proteomic databases

MaxQBiP07213.
PRIDEiP07213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL121C; YNL121C; YNL121C.
GeneIDi855602.
KEGGisce:YNL121C.

Organism-specific databases

EuPathDBiFungiDB:YNL121C.
SGDiS000005065. TOM70.

Phylogenomic databases

GeneTreeiENSGT00860000133798.
InParanoidiP07213.
KOiK17768.
OMAiCITYKNG.
OrthoDBiEOG092C1Q9V.

Enzyme and pathway databases

BioCyciYEAST:G3O-33142-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP07213.
PROiP07213.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005687. Tom70.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 8 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
TIGRFAMsiTIGR00990. 3a0801s09. 1 hit.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOM70_YEAST
AccessioniPrimary (citable) accession number: P07213
Secondary accession number(s): B0KZR5
, B0KZS4, B0KZU2, B0KZY7, B0KZZ6, B0L005, B0L023, D6W162
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 186 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 45300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.