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P07213 (TOM70_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 160. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import receptor subunit TOM70
Alternative name(s):
70 kDa mitochondrial outer membrane protein
Translocase of outer membrane 70 kDa subunit
Gene names
Name:TOM70
Synonyms:MAS70, OMP1
Ordered Locus Names:YNL121C
ORF Names:N1905
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length617 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Ref.11

Subunit structure

Forms part of the TOM (translocase of outer membrane) complex that consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex interacts with TOM22. Interacts with TOM37. Ref.9 Ref.10

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein Ref.12.

Miscellaneous

Present with 45300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Tom70 family.

Contains 9 TPR repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BAXQ078122EBI-12551,EBI-516580From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 617617Mitochondrial import receptor subunit TOM70
PRO_0000106340

Regions

Topological domain1 – 1010Mitochondrial intermembrane Potential
Transmembrane11 – 3020Helical; Potential
Topological domain31 – 617587Cytoplasmic Potential
Repeat99 – 13234TPR 1
Repeat134 – 16532TPR 2
Repeat281 – 31535TPR 3
Repeat363 – 39634TPR 4
Repeat397 – 43034TPR 5
Repeat432 – 46433TPR 6
Repeat465 – 49834TPR 7
Repeat505 – 54137TPR 8
Repeat542 – 57534TPR 9

Amino acid modifications

Modified residue1741Phosphoserine Ref.15

Natural variations

Natural variant301N → S in strain: SK1, V1-09, YJM339 and YJM627. Ref.2
Natural variant681P → S in strain: V1-09 and YJM627. Ref.2
Natural variant2771N → H in strain: YJM1129. Ref.2
Natural variant3591L → S in strain: SK1. Ref.2
Natural variant3641N → I in strain: YJM269 and YJM270. Ref.2
Natural variant3851Y → H in strain: YJM1129. Ref.2
Natural variant4291E → G in ytrain: V1-09. Ref.2

Experimental info

Sequence conflict1851A → R in CAA29085. Ref.1

Secondary structure

............................................................. 617
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07213 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 664B8FC32CCE39A2

FASTA61770,123
        10         20         30         40         50         60 
MKSFITRNKT AILATVAATG TAIGAYYYYN QLQQQQQRGK KNTINKDEKK DTKDSQKETE 

        70         80         90        100        110        120 
GAKKSTAPSN PPIYPVSSNG EPDFSNKANF TAEEKDKYAL ALKDKGNQFF RNKKYDDAIK 

       130        140        150        160        170        180 
YYNWALELKE DPVFYSNLSA CYVSVGDLKK VVEMSTKALE LKPDYSKVLL RRASANEGLG 

       190        200        210        220        230        240 
KFADAMFDLS VLSLNGDFND ASIEPMLERN LNKQAMSKLK EKFGDIDTAT ATPTELSTQP 

       250        260        270        280        290        300 
AKERKDKQEN LPSVTSMASF FGIFKPELTF ANYDESNEAD KELMNGLSNL YKRSPESYDK 

       310        320        330        340        350        360 
ADESFTKAAR LFEEQLDKNN EDEKLKEKLA ISLEHTGIFK FLKNDPLGAH EDIKKAIELF 

       370        380        390        400        410        420 
PRVNSYIYMA LIMADRNDST EYYNYFDKAL KLDSNNSSVY YHRGQMNFIL QNYDQAGKDF 

       430        440        450        460        470        480 
DKAKELDPEN IFPYIQLACL AYRENKFDDC ETLFSEAKRK FPEAPEVPNF FAEILTDKND 

       490        500        510        520        530        540 
FDKALKQYDL AIELENKLDG IYVGIAPLVG KATLLTRNPT VENFIEATNL LEKASKLDPR 

       550        560        570        580        590        600 
SEQAKIGLAQ MKLQQEDIDE AITLFEESAD LARTMEEKLQ AITFAEAAKV QQRIRSDPVL 

       610 
AKKIQETLAK LREQGLM 

« Hide

References

« Hide 'large scale' references
[1]"Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane."
Hase T., Riezman H., Suda K., Schatz G.
EMBO J. 2:2169-2172(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-30; SER-68; HIS-277; SER-359; ILE-364; HIS-385 AND GLY-429.
Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
[3]"The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Expanding family."
Boguski M.S., Sikorski R.S., Hieter P.A., Goebl M.
Nature 346:114-114(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAINS TPR REPEATS.
[7]"Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70."
Hines V., Brandt A., Griffiths G., Horstmann H., Brutsch H., Schatz G.
EMBO J. 9:3191-3200(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Precursor binding to yeast mitochondria. A general role for the outer membrane protein Mas70p."
Hines V., Schatz G.
J. Biol. Chem. 268:449-454(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[9]"Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TOM COMPLEX.
[10]"Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase."
van Wilpe S., Ryan M.T., Hill K., Maarse A.C., Meisinger C., Brix J., Dekker P.J.T., Moczko M., Wagner R., Meijer M., Guiard B., Hoenlinger A., Pfanner N.
Nature 401:485-489(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TOM22.
[11]"The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins."
Brix J., Ziegler G.A., Dietmeier K., Schneider-Mergener J., Schulz G.E., Pfanner N.
J. Mol. Biol. 303:479-488(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[15]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05585 Genomic DNA. Translation: CAA29085.1.
EF125216 Genomic DNA. Translation: ABN58537.1.
EF125217 Genomic DNA. Translation: ABN58546.1.
EF125218 Genomic DNA. Translation: ABN58555.1.
EF125219 Genomic DNA. Translation: ABN58564.1.
EF125220 Genomic DNA. Translation: ABN58573.1.
EF125221 Genomic DNA. Translation: ABN58582.1.
EF125222 Genomic DNA. Translation: ABN58591.1.
EF125223 Genomic DNA. Translation: ABN58600.1.
EF125224 Genomic DNA. Translation: ABN58609.1.
EF125225 Genomic DNA. Translation: ABN58618.1.
EF125226 Genomic DNA. Translation: ABN58627.1.
EF125228 Genomic DNA. Translation: ABN58645.1.
Z69382 Genomic DNA. Translation: CAA93386.1.
Z71397 Genomic DNA. Translation: CAA96002.1.
BK006947 Genomic DNA. Translation: DAA10428.1.
PIRMMBYO. S63062.
RefSeqNP_014278.3. NM_001182959.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GW1X-ray3.00A/B94-607[»]
ProteinModelPortalP07213.
SMRP07213. Positions 94-607.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35706. 156 interactions.
DIPDIP-2301N.
IntActP07213. 12 interactions.
MINTMINT-508943.
STRING4932.YNL121C.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

PaxDbP07213.
PeptideAtlasP07213.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL121C; YNL121C; YNL121C.
GeneID855602.
KEGGsce:YNL121C.

Organism-specific databases

CYGDYNL121c.
SGDS000005065. TOM70.

Phylogenomic databases

eggNOGNOG290605.
GeneTreeENSGT00750000117222.
KOK17768.
OMAKTGDGYE.
OrthoDBEOG7R839F.

Enzyme and pathway databases

BioCycYEAST:G3O-33142-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

GenevestigatorP07213.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR005687. Tom70.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamPF00515. TPR_1. 2 hits.
[Graphical view]
SMARTSM00028. TPR. 6 hits.
[Graphical view]
TIGRFAMsTIGR00990. 3a0801s09. 1 hit.
PROSITEPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07213.
NextBio979768.

Entry information

Entry nameTOM70_YEAST
AccessionPrimary (citable) accession number: P07213
Secondary accession number(s): B0KZR5 expand/collapse secondary AC list , B0KZS4, B0KZU2, B0KZY7, B0KZZ6, B0L005, B0L023, D6W162
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references