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P07213

- TOM70_YEAST

UniProt

P07213 - TOM70_YEAST

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Protein

Mitochondrial import receptor subunit TOM70

Gene
TOM70, MAS70, OMP1, YNL121C, N1905
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.1 Publication

GO - Molecular functioni

  1. mitochondrion targeting sequence binding Source: SGD
  2. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  3. protein binding Source: IntAct

GO - Biological processi

  1. protein import into mitochondrial inner membrane Source: SGD
  2. protein import into mitochondrial matrix Source: SGD
  3. protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33142-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM70
Alternative name(s):
70 kDa mitochondrial outer membrane protein
Translocase of outer membrane 70 kDa subunit
Gene namesi
Name:TOM70
Synonyms:MAS70, OMP1
Ordered Locus Names:YNL121C
ORF Names:N1905
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL121c.
SGDiS000005065. TOM70.

Subcellular locationi

Mitochondrion outer membrane; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010Mitochondrial intermembrane Reviewed prediction
Transmembranei11 – 3020Helical; Reviewed predictionAdd
BLAST
Topological domaini31 – 617587Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: SGD
  2. mitochondrial outer membrane Source: Reactome
  3. mitochondrial outer membrane translocase complex Source: SGD
  4. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 617617Mitochondrial import receptor subunit TOM70PRO_0000106340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP07213.
PaxDbiP07213.
PeptideAtlasiP07213.

Expressioni

Gene expression databases

GenevestigatoriP07213.

Interactioni

Subunit structurei

Forms part of the TOM (translocase of outer membrane) complex that consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex interacts with TOM22. Interacts with TOM37.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BAXQ078122EBI-12551,EBI-516580From a different organism.

Protein-protein interaction databases

BioGridi35706. 157 interactions.
DIPiDIP-2301N.
IntActiP07213. 12 interactions.
MINTiMINT-508943.
STRINGi4932.YNL121C.

Structurei

Secondary structure

1
617
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi95 – 11117
Helixi115 – 12814
Helixi132 – 14514
Helixi148 – 16114
Helixi166 – 17813
Helixi182 – 19413
Beta strandi195 – 1973
Helixi200 – 2023
Helixi204 – 21916
Helixi254 – 2618
Helixi278 – 29013
Helixi297 – 31519
Turni316 – 3183
Helixi323 – 34220
Helixi346 – 35914
Helixi363 – 37412
Helixi382 – 3843
Helixi385 – 3906
Helixi398 – 40912
Helixi415 – 42511
Helixi432 – 4409
Turni441 – 4455
Helixi447 – 46014
Helixi466 – 47712
Helixi481 – 49616
Beta strandi498 – 5003
Helixi506 – 51611
Helixi523 – 53715
Helixi542 – 55413
Helixi558 – 57114
Helixi575 – 59420
Helixi598 – 6058

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GW1X-ray3.00A/B94-607[»]
ProteinModelPortaliP07213.
SMRiP07213. Positions 94-607.

Miscellaneous databases

EvolutionaryTraceiP07213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati99 – 13234TPR 1Add
BLAST
Repeati134 – 16532TPR 2Add
BLAST
Repeati281 – 31535TPR 3Add
BLAST
Repeati363 – 39634TPR 4Add
BLAST
Repeati397 – 43034TPR 5Add
BLAST
Repeati432 – 46433TPR 6Add
BLAST
Repeati465 – 49834TPR 7Add
BLAST
Repeati505 – 54137TPR 8Add
BLAST
Repeati542 – 57534TPR 9Add
BLAST

Sequence similaritiesi

Belongs to the Tom70 family.
Contains 9 TPR repeats.

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290605.
GeneTreeiENSGT00750000117222.
KOiK17768.
OMAiCILEAFQ.
OrthoDBiEOG7R839F.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005687. Tom70.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 2 hits.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
TIGRFAMsiTIGR00990. 3a0801s09. 1 hit.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07213-1 [UniParc]FASTAAdd to Basket

« Hide

MKSFITRNKT AILATVAATG TAIGAYYYYN QLQQQQQRGK KNTINKDEKK    50
DTKDSQKETE GAKKSTAPSN PPIYPVSSNG EPDFSNKANF TAEEKDKYAL 100
ALKDKGNQFF RNKKYDDAIK YYNWALELKE DPVFYSNLSA CYVSVGDLKK 150
VVEMSTKALE LKPDYSKVLL RRASANEGLG KFADAMFDLS VLSLNGDFND 200
ASIEPMLERN LNKQAMSKLK EKFGDIDTAT ATPTELSTQP AKERKDKQEN 250
LPSVTSMASF FGIFKPELTF ANYDESNEAD KELMNGLSNL YKRSPESYDK 300
ADESFTKAAR LFEEQLDKNN EDEKLKEKLA ISLEHTGIFK FLKNDPLGAH 350
EDIKKAIELF PRVNSYIYMA LIMADRNDST EYYNYFDKAL KLDSNNSSVY 400
YHRGQMNFIL QNYDQAGKDF DKAKELDPEN IFPYIQLACL AYRENKFDDC 450
ETLFSEAKRK FPEAPEVPNF FAEILTDKND FDKALKQYDL AIELENKLDG 500
IYVGIAPLVG KATLLTRNPT VENFIEATNL LEKASKLDPR SEQAKIGLAQ 550
MKLQQEDIDE AITLFEESAD LARTMEEKLQ AITFAEAAKV QQRIRSDPVL 600
AKKIQETLAK LREQGLM 617
Length:617
Mass (Da):70,123
Last modified:October 1, 1996 - v2
Checksum:i664B8FC32CCE39A2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301N → S in strain: SK1, V1-09, YJM339 and YJM627. 1 Publication
Natural varianti68 – 681P → S in strain: V1-09 and YJM627. 1 Publication
Natural varianti277 – 2771N → H in strain: YJM1129. 1 Publication
Natural varianti359 – 3591L → S in strain: SK1. 1 Publication
Natural varianti364 – 3641N → I in strain: YJM269 and YJM270. 1 Publication
Natural varianti385 – 3851Y → H in strain: YJM1129. 1 Publication
Natural varianti429 – 4291E → G in ytrain: V1-09. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851A → R in CAA29085. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05585 Genomic DNA. Translation: CAA29085.1.
EF125216 Genomic DNA. Translation: ABN58537.1.
EF125217 Genomic DNA. Translation: ABN58546.1.
EF125218 Genomic DNA. Translation: ABN58555.1.
EF125219 Genomic DNA. Translation: ABN58564.1.
EF125220 Genomic DNA. Translation: ABN58573.1.
EF125221 Genomic DNA. Translation: ABN58582.1.
EF125222 Genomic DNA. Translation: ABN58591.1.
EF125223 Genomic DNA. Translation: ABN58600.1.
EF125224 Genomic DNA. Translation: ABN58609.1.
EF125225 Genomic DNA. Translation: ABN58618.1.
EF125226 Genomic DNA. Translation: ABN58627.1.
EF125228 Genomic DNA. Translation: ABN58645.1.
Z69382 Genomic DNA. Translation: CAA93386.1.
Z71397 Genomic DNA. Translation: CAA96002.1.
BK006947 Genomic DNA. Translation: DAA10428.1.
PIRiS63062. MMBYO.
RefSeqiNP_014278.3. NM_001182959.3.

Genome annotation databases

EnsemblFungiiYNL121C; YNL121C; YNL121C.
GeneIDi855602.
KEGGisce:YNL121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05585 Genomic DNA. Translation: CAA29085.1 .
EF125216 Genomic DNA. Translation: ABN58537.1 .
EF125217 Genomic DNA. Translation: ABN58546.1 .
EF125218 Genomic DNA. Translation: ABN58555.1 .
EF125219 Genomic DNA. Translation: ABN58564.1 .
EF125220 Genomic DNA. Translation: ABN58573.1 .
EF125221 Genomic DNA. Translation: ABN58582.1 .
EF125222 Genomic DNA. Translation: ABN58591.1 .
EF125223 Genomic DNA. Translation: ABN58600.1 .
EF125224 Genomic DNA. Translation: ABN58609.1 .
EF125225 Genomic DNA. Translation: ABN58618.1 .
EF125226 Genomic DNA. Translation: ABN58627.1 .
EF125228 Genomic DNA. Translation: ABN58645.1 .
Z69382 Genomic DNA. Translation: CAA93386.1 .
Z71397 Genomic DNA. Translation: CAA96002.1 .
BK006947 Genomic DNA. Translation: DAA10428.1 .
PIRi S63062. MMBYO.
RefSeqi NP_014278.3. NM_001182959.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GW1 X-ray 3.00 A/B 94-607 [» ]
ProteinModelPortali P07213.
SMRi P07213. Positions 94-607.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35706. 157 interactions.
DIPi DIP-2301N.
IntActi P07213. 12 interactions.
MINTi MINT-508943.
STRINGi 4932.YNL121C.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi P07213.
PaxDbi P07213.
PeptideAtlasi P07213.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL121C ; YNL121C ; YNL121C .
GeneIDi 855602.
KEGGi sce:YNL121C.

Organism-specific databases

CYGDi YNL121c.
SGDi S000005065. TOM70.

Phylogenomic databases

eggNOGi NOG290605.
GeneTreei ENSGT00750000117222.
KOi K17768.
OMAi CILEAFQ.
OrthoDBi EOG7R839F.

Enzyme and pathway databases

BioCyci YEAST:G3O-33142-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTracei P07213.
NextBioi 979768.

Gene expression databases

Genevestigatori P07213.

Family and domain databases

Gene3Di 1.25.40.10. 3 hits.
InterProi IPR005687. Tom70.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view ]
Pfami PF00515. TPR_1. 2 hits.
[Graphical view ]
SMARTi SM00028. TPR. 6 hits.
[Graphical view ]
TIGRFAMsi TIGR00990. 3a0801s09. 1 hit.
PROSITEi PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane."
    Hase T., Riezman H., Suda K., Schatz G.
    EMBO J. 2:2169-2172(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast."
    Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S., Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H., Steinmetz L.M.
    Genetics 180:1661-1670(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-30; SER-68; HIS-277; SER-359; ILE-364; HIS-385 AND GLY-429.
    Strain: ATCC 200060 / W303, S103, SK1, V1-09, YJM 1129, YJM 269, YJM 270, YJM 320, YJM 326, YJM 339, YJM 627 and YJM230.
  3. "The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames."
    de Antoni A., D'Angelo M., Dal Pero F., Sartorello F., Pandolfo D., Pallavicini A., Lanfranchi G., Valle G.
    Yeast 13:261-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: DOMAINS TPR REPEATS.
  7. "Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70."
    Hines V., Brandt A., Griffiths G., Horstmann H., Brutsch H., Schatz G.
    EMBO J. 9:3191-3200(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Precursor binding to yeast mitochondria. A general role for the outer membrane protein Mas70p."
    Hines V., Schatz G.
    J. Biol. Chem. 268:449-454(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
    Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
    Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TOM COMPLEX.
  10. Cited for: INTERACTION WITH TOM22.
  11. "The mitochondrial import receptor Tom70: identification of a 25 kDa core domain with a specific binding site for preproteins."
    Brix J., Ziegler G.A., Dietmeier K., Schneider-Mergener J., Schulz G.E., Pfanner N.
    J. Mol. Biol. 303:479-488(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTOM70_YEAST
AccessioniPrimary (citable) accession number: P07213
Secondary accession number(s): B0KZR5
, B0KZS4, B0KZU2, B0KZY7, B0KZZ6, B0L005, B0L023, D6W162
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 45300 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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