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Protein

Pullulanase

Gene

pulA

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei684NucleophileBy similarity1
Active sitei713Proton donorBy similarity1
Sitei841Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiCBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Pullulanase (EC:3.2.1.41)
Alternative name(s):
Alpha-dextrin endo-1,6-alpha-glucosidase
Pullulan 6-glucanohydrolase
Gene namesi
Name:pulA
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19PROSITE-ProRule annotationAdd BLAST19
ChainiPRO_000000142720 – 1090PullulanaseAdd BLAST1071

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi20S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

11090
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 66Combined sources7
Helixi68 – 71Combined sources4
Beta strandi80 – 87Combined sources8
Beta strandi95 – 97Combined sources3
Beta strandi118 – 124Combined sources7
Beta strandi126 – 144Combined sources19
Beta strandi146 – 149Combined sources4
Turni150 – 152Combined sources3
Beta strandi157 – 161Combined sources5
Beta strandi168 – 170Combined sources3
Helixi171 – 178Combined sources8
Beta strandi186 – 188Combined sources3
Beta strandi190 – 195Combined sources6
Helixi197 – 199Combined sources3
Beta strandi203 – 213Combined sources11
Beta strandi221 – 223Combined sources3
Beta strandi231 – 233Combined sources3
Helixi237 – 242Combined sources6
Helixi244 – 246Combined sources3
Beta strandi251 – 253Combined sources3
Helixi260 – 263Combined sources4
Beta strandi266 – 273Combined sources8
Beta strandi278 – 286Combined sources9
Helixi288 – 299Combined sources12
Beta strandi305 – 309Combined sources5
Beta strandi312 – 318Combined sources7
Beta strandi323 – 330Combined sources8
Beta strandi336 – 341Combined sources6
Beta strandi343 – 345Combined sources3
Turni346 – 349Combined sources4
Beta strandi350 – 355Combined sources6
Helixi357 – 359Combined sources3
Beta strandi363 – 371Combined sources9
Turni373 – 375Combined sources3
Beta strandi377 – 383Combined sources7
Beta strandi389 – 391Combined sources3
Helixi393 – 395Combined sources3
Beta strandi397 – 399Combined sources3
Helixi406 – 408Combined sources3
Helixi425 – 429Combined sources5
Beta strandi432 – 436Combined sources5
Helixi438 – 442Combined sources5
Helixi450 – 452Combined sources3
Helixi456 – 460Combined sources5
Helixi465 – 476Combined sources12
Beta strandi480 – 484Combined sources5
Beta strandi487 – 492Combined sources6
Helixi496 – 498Combined sources3
Helixi506 – 512Combined sources7
Helixi514 – 517Combined sources4
Helixi522 – 525Combined sources4
Beta strandi526 – 528Combined sources3
Helixi531 – 539Combined sources9
Helixi548 – 556Combined sources9
Beta strandi559 – 561Combined sources3
Beta strandi568 – 574Combined sources7
Helixi586 – 600Combined sources15
Beta strandi605 – 610Combined sources6
Beta strandi613 – 616Combined sources4
Turni619 – 621Combined sources3
Helixi627 – 630Combined sources4
Turni632 – 634Combined sources3
Turni640 – 642Combined sources3
Beta strandi650 – 654Combined sources5
Helixi659 – 674Combined sources16
Beta strandi680 – 683Combined sources4
Helixi686 – 688Combined sources3
Helixi691 – 702Combined sources12
Beta strandi709 – 712Combined sources4
Turni720 – 722Combined sources3
Turni728 – 734Combined sources7
Beta strandi737 – 739Combined sources3
Helixi741 – 748Combined sources8
Helixi758 – 761Combined sources4
Turni765 – 771Combined sources7
Helixi781 – 795Combined sources15
Beta strandi799 – 801Combined sources3
Beta strandi803 – 805Combined sources3
Beta strandi811 – 813Combined sources3
Helixi814 – 816Combined sources3
Beta strandi817 – 819Combined sources3
Beta strandi822 – 824Combined sources3
Beta strandi827 – 829Combined sources3
Helixi830 – 832Combined sources3
Beta strandi833 – 835Combined sources3
Beta strandi840 – 842Combined sources3
Helixi845 – 852Combined sources8
Helixi859 – 874Combined sources16
Beta strandi876 – 883Combined sources8
Helixi886 – 888Combined sources3
Helixi898 – 900Combined sources3
Helixi902 – 905Combined sources4
Beta strandi918 – 920Combined sources3
Helixi924 – 927Combined sources4
Helixi928 – 930Combined sources3
Helixi931 – 938Combined sources8
Helixi946 – 964Combined sources19
Helixi967 – 969Combined sources3
Helixi974 – 980Combined sources7
Beta strandi981 – 985Combined sources5
Beta strandi994 – 1000Combined sources7
Turni1003 – 1005Combined sources3
Beta strandi1011 – 1021Combined sources11
Beta strandi1023 – 1025Combined sources3
Beta strandi1027 – 1029Combined sources3
Turni1031 – 1034Combined sources4
Helixi1041 – 1046Combined sources6
Helixi1047 – 1049Combined sources3
Turni1051 – 1054Combined sources4
Beta strandi1063 – 1065Combined sources3
Beta strandi1067 – 1076Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YOCX-ray2.88A/B21-1089[»]
ProteinModelPortaliP07206.
SMRiP07206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07206.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd10315. CBM41_pullulanase. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 4 hits.
InterProiIPR013784. Carb-bd-like_fold.
IPR005323. CBM41_pullulanase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR011839. Pullul_strch.
IPR024561. Pullul_strch_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 5 hits.
PfamiPF02922. CBM_48. 1 hit.
PF11852. DUF3372. 1 hit.
PF03714. PUD. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR02103. pullul_strch. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRYTRNALV LGSLVLLSGC DNGSSSSSSG NPDTPDNQDV VVRLPDVAVP
60 70 80 90 100
GEAVTAVENQ AVIHLVDIAG ITSSSAADYS SKNLYLWNNE TCDALSAPVA
110 120 130 140 150
DWNDVSTTPS GSDKYGPYWV IPLNKESGCI NVIVRDGTDK LIDSDLRVAF
160 170 180 190 200
GDFTDRTVSV IAGNSAVYDS RADAFRAAFG VALAEAHWVD KNTLLWPGGQ
210 220 230 240 250
DKPIVRLYYS HSSKVAADGE GKFTDRYLKL TPTTVSQQVS MRFPHLSSYA
260 270 280 290 300
AFKLPDNANV DELLQGETVA IAAAEDGILI SATQVQTAGV LDDAYAEAAE
310 320 330 340 350
ALSYGAQLAD GGVTFRVWAP TAQQVDVVVY SADKKVIGSH PMTRDSASGA
360 370 380 390 400
WSWQGGSDLK GAFYRYAMTV YHPQSRKVEQ YEVTDPYAHS LSTNSEYSQV
410 420 430 440 450
VDLNDSALKP DGWDNLTMPH AQKTKADLAK MTIHESHIRD LSAWDQTVPA
460 470 480 490 500
ELRGKYLALT AGDSNMVQHL KTLSASGVTH VELLPVFDLA TVNEFSDKVA
510 520 530 540 550
DIQQPFSRLC EVNSAVKSSE FAGYCDSGST VEEVLNQLKQ SDSQDNPQVQ
560 570 580 590 600
ALNTLVAQTD SYNWGYDPFH YTVPEGSYAT DPEGTTRIKE FRTMIQAIKQ
610 620 630 640 650
DLGMNVIMDV VYNHTNAAGP TDRTSVLDKI VPWYYQRLNE TTGSVESATC
660 670 680 690 700
CSDSAPEHRM FAKLIADSLA VWTTDYKIDG FRFDLMGYHP KAQILSAWER
710 720 730 740 750
IKALNPDIYF FGEGWDSNQS DRFEIASQIN LKGTGIGTFS DRLRDSVRGG
760 770 780 790 800
GPFDSGDALR QNQGIGSGAG VLPNELASLS DDQVRHLADL TRLGMAGNLA
810 820 830 840 850
DFVMIDKDGA AKKGSEIDYN GAPGGYAADP TEVVNYVSKH DNQTLWDMIS
860 870 880 890 900
YKASQEADLA TRVRMQAVSL ATVMLGQGIA FDQQGSELLR SKSFTRDSYD
910 920 930 940 950
SGDWFNRVDY SLQDNNYNVG MPRISDDGSN YEVITRVKEM VATPGEAELK
960 970 980 990 1000
QMTAFYQELT ELRKSSPLFT LGDGSAVMKR VDFRNTGSDQ QAGLLVMTVD
1010 1020 1030 1040 1050
DGMKAGASLD SRLDGLVVAI NAAPESRTLN EFAGETLQLS AIQQTAGENS
1060 1070 1080 1090
LANGVQIAAD GTVTLPAWSV AVLELPQGEA QGAGLPVSSK
Length:1,090
Mass (Da):118,098
Last modified:April 1, 1990 - v2
Checksum:i240AE7DFB3FF1BD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6R → C in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti10V → F in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti15V → I in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti23G → S in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti31N → S in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti34T → N in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti36D → DGNP in AAA25087 (PubMed:3902792).Curated1
Sequence conflicti55 – 58TAVE → MATA in AAA25087 (PubMed:3902792).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52181 Genomic DNA. Translation: CAA36431.1.
M12503 Genomic DNA. Translation: AAA25087.2.
M29097 Genomic DNA. Translation: AAA61976.1.
PIRiS11823.
S38801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52181 Genomic DNA. Translation: CAA36431.1.
M12503 Genomic DNA. Translation: AAA25087.2.
M29097 Genomic DNA. Translation: AAA61976.1.
PIRiS11823.
S38801.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YOCX-ray2.88A/B21-1089[»]
ProteinModelPortaliP07206.
SMRiP07206.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP07206.

Family and domain databases

CDDicd10315. CBM41_pullulanase. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 4 hits.
InterProiIPR013784. Carb-bd-like_fold.
IPR005323. CBM41_pullulanase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR011839. Pullul_strch.
IPR024561. Pullul_strch_C.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 5 hits.
PfamiPF02922. CBM_48. 1 hit.
PF11852. DUF3372. 1 hit.
PF03714. PUD. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR02103. pullul_strch. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPULA_KLEPN
AccessioniPrimary (citable) accession number: P07206
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1990
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.