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Protein

Phosphoglycerate kinase 2

Gene

PGK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity).By similarity1 Publication

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-162eP), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (HEL-S-278), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS)
  2. Phosphoglycerate kinase 2 (PGK2), Phosphoglycerate kinase 1 (PGK1)
  3. no protein annotated in this organism
  4. Alpha-enolase (ENO1), Beta-enolase (ENO3), Enolase 4 (ENO4), Gamma-enolase (ENO2)
  5. Pyruvate kinase, Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase (PKM), Pyruvate kinase, Pyruvate kinase PKLR (PKLR), Pyruvate kinase, Pyruvate kinase (PKM2), Pyruvate kinase (PKM), Pyruvate kinase (HEL-S-30), Pyruvate kinase (PKM2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei313ATP; via carbonyl oxygenBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • flagellated sperm motility Source: Ensembl
  • glycolytic process Source: UniProtKB
  • phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS10215-MONOMER.
ZFISH:HS10215-MONOMER.
ReactomeiR-HSA-70171. Glycolysis.
R-HSA-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 2 (EC:2.7.2.3)
Alternative name(s):
Phosphoglycerate kinase, testis specific
Gene namesi
Name:PGK2
Synonyms:PGKB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:8898. PGK2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
  • sperm fibrous sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000170950.
PharmGKBiPA33237.

Chemistry databases

ChEMBLiCHEMBL2096677.

Polymorphism and mutation databases

BioMutaiPGK2.
DMDMi21264485.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001458322 – 417Phosphoglycerate kinase 2Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineBy similarity1
Modified residuei11N6-acetyllysineBy similarity1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineBy similarity1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineBy similarity1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysineBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineBy similarity1
Modified residuei361N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP07205.
MaxQBiP07205.
PaxDbiP07205.
PeptideAtlasiP07205.
PRIDEiP07205.

2D gel databases

UCD-2DPAGEP07205.

PTM databases

iPTMnetiP07205.
PhosphoSitePlusiP07205.
SwissPalmiP07205.

Expressioni

Tissue specificityi

Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific. Expression significantly decreased in the testis of elderly men.1 Publication

Gene expression databases

BgeeiENSG00000170950.
CleanExiHS_PGK2.
ExpressionAtlasiP07205. baseline and differential.
GenevisibleiP07205. HS.

Organism-specific databases

HPAiHPA045385.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi111253. 31 interactors.
STRINGi9606.ENSP00000305995.

Structurei

3D structure databases

ProteinModelPortaliP07205.
SMRiP07205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP07205.
KOiK00927.
OMAiKGCITVI.
OrthoDBiEOG091G08S6.
PhylomeDBiP07205.
TreeFamiTF300489.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC
60 70 80 90 100
LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV
110 120 130 140 150
GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA
210 220 230 240 250
LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN
260 270 280 290 300
MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK
310 320 330 340 350
ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK
360 370 380 390 400
GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKILPG VEALSNM
Length:417
Mass (Da):44,796
Last modified:January 23, 2007 - v3
Checksum:i0CD5C71C2D3A9272
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti396G → R in CAA28872 (PubMed:3453121).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05246 Genomic DNA. Translation: CAA28872.1.
AK312770 mRNA. Translation: BAG35635.1.
AL121974 Genomic DNA. Translation: CAC19655.1.
BC038843 mRNA. Translation: AAH38843.1.
CCDSiCCDS4930.1.
PIRiA24030.
A27816.
RefSeqiNP_620061.2. NM_138733.4.
UniGeneiHs.367727.

Genome annotation databases

EnsembliENST00000304801; ENSP00000305995; ENSG00000170950.
GeneIDi5232.
KEGGihsa:5232.
UCSCiuc003ozu.4. human.

Cross-referencesi

Web resourcesi

Wikipedia

Phosphoglycerate kinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05246 Genomic DNA. Translation: CAA28872.1.
AK312770 mRNA. Translation: BAG35635.1.
AL121974 Genomic DNA. Translation: CAC19655.1.
BC038843 mRNA. Translation: AAH38843.1.
CCDSiCCDS4930.1.
PIRiA24030.
A27816.
RefSeqiNP_620061.2. NM_138733.4.
UniGeneiHs.367727.

3D structure databases

ProteinModelPortaliP07205.
SMRiP07205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111253. 31 interactors.
STRINGi9606.ENSP00000305995.

Chemistry databases

ChEMBLiCHEMBL2096677.

PTM databases

iPTMnetiP07205.
PhosphoSitePlusiP07205.
SwissPalmiP07205.

Polymorphism and mutation databases

BioMutaiPGK2.
DMDMi21264485.

2D gel databases

UCD-2DPAGEP07205.

Proteomic databases

EPDiP07205.
MaxQBiP07205.
PaxDbiP07205.
PeptideAtlasiP07205.
PRIDEiP07205.

Protocols and materials databases

DNASUi5232.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304801; ENSP00000305995; ENSG00000170950.
GeneIDi5232.
KEGGihsa:5232.
UCSCiuc003ozu.4. human.

Organism-specific databases

CTDi5232.
GeneCardsiPGK2.
HGNCiHGNC:8898. PGK2.
HPAiHPA045385.
MIMi172270. gene.
neXtProtiNX_P07205.
OpenTargetsiENSG00000170950.
PharmGKBiPA33237.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1367. Eukaryota.
COG0126. LUCA.
GeneTreeiENSGT00390000008820.
HOGENOMiHOG000227107.
HOVERGENiHBG008177.
InParanoidiP07205.
KOiK00927.
OMAiKGCITVI.
OrthoDBiEOG091G08S6.
PhylomeDBiP07205.
TreeFamiTF300489.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciMetaCyc:HS10215-MONOMER.
ZFISH:HS10215-MONOMER.
ReactomeiR-HSA-70171. Glycolysis.
R-HSA-70263. Gluconeogenesis.

Miscellaneous databases

GenomeRNAii5232.
PROiP07205.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170950.
CleanExiHS_PGK2.
ExpressionAtlasiP07205. baseline and differential.
GenevisibleiP07205. HS.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK2_HUMAN
AccessioniPrimary (citable) accession number: P07205
Secondary accession number(s): B2R6Y8, Q9H107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.