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P07201

- RIR2_SPISO

UniProt

P07201 - RIR2_SPISO

Protein

Ribonucleoside-diphosphate reductase small chain

Gene
N/A
Organism
Spisula solidissima (Atlantic surf-clam)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi130 – 1301Iron 1PROSITE-ProRule annotation
    Metal bindingi161 – 1611Iron 1PROSITE-ProRule annotation
    Metal bindingi161 – 1611Iron 2By similarity
    Metal bindingi164 – 1641Iron 1PROSITE-ProRule annotation
    Active sitei168 – 1681PROSITE-ProRule annotation
    Metal bindingi224 – 2241Iron 2By similarity
    Metal bindingi258 – 2581Iron 2By similarity
    Metal bindingi261 – 2611Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small chain (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    p41
    OrganismiSpisula solidissima (Atlantic surf-clam)
    Taxonomic identifieri6584 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaHeteroconchiaVeneroidaMactroideaMactridaeSpisula

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384Ribonucleoside-diphosphate reductase small chainPRO_0000190453Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.

    Structurei

    3D structure databases

    ProteinModelPortaliP07201.
    SMRiP07201. Positions 3-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P07201-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSINTTRKE NELSGNLGKM KITEENKPKK VLGEITNFQR STQKTPLKQE    50
    IKPVVKKSQQ VEPLLADNPR RFVVLPIQYH DIWKMYKKAE ASFWTAEEVD 100
    LSKDMAHWES LKKEEKHFIS HVLAFFAASD GIVNENLVER FSKEVQVTEA 150
    RCFYGFQIAM ENIHSEMYSL LIDTYIKDPQ ERDFLFNAIE TMPCVKEKAD 200
    WAMRWINDDS SSYAERVVAF AAVEGIFFSG SFASIFWLKK RGIMPGLTFS 250
    NELISRDEGL HCDFACLMFS HLVNKPSQER IHQIIDEAVK IEQVFLTEAL 300
    PCRLIGMNCD LMRQYIEFVA DRLLLELKCD KLYNKENPFD FMEHISLEGK 350
    TNFFEKRVGE YQKMGVMSGG NTGDSHAFTL DADF 384
    Length:384
    Mass (Da):44,447
    Last modified:April 1, 1993 - v2
    Checksum:i2F576F7BEA9297B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55125 mRNA. Translation: CAA38919.1.
    X02471 mRNA. Translation: CAA26307.1.
    PIRiA22259. RDSS2R.
    S24585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55125 mRNA. Translation: CAA38919.1 .
    X02471 mRNA. Translation: CAA26307.1 .
    PIRi A22259. RDSS2R.
    S24585.

    3D structure databases

    ProteinModelPortali P07201.
    SMRi P07201. Positions 3-344.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Maternal mRNA from clam oocytes can be specifically unmasked in vitro by antisense RNA complementary to the 3'-untranslated region."
      Standart N.M., Dale M., Stewart E., Hunt T.
      Genes Dev. 4:2157-2168(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The small subunit of ribonucleotide reductase is encoded by one of the most abundant translationally regulated maternal RNAs in clam and sea urchin eggs."
      Standart N.M., Bray S.J., George E.L., Hunt T., Ruderman J.V.
      J. Cell Biol. 100:1968-1976(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 85-384.

    Entry informationi

    Entry nameiRIR2_SPISO
    AccessioniPrimary (citable) accession number: P07201
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    There is no sign of synthesis of a corresponding large subunit after fertilization. The authors suspect that the unfertilized oocytes contain a stockpile of large subunits ready for combination with newly made small subunits.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3