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Protein

Major centromere autoantigen B

Gene

CENPB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed:11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).Curated1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 48H-T-H motifAdd BLAST21
DNA bindingi97 – 129H-T-H motifAdd BLAST33

GO - Molecular functioni

  • centromeric DNA binding Source: BHF-UCL
  • chromatin binding Source: UniProtKB
  • satellite DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: BHF-UCL

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Major centromere autoantigen B
Alternative name(s):
Centromere protein B
Short name:
CENP-B
Gene namesi
Name:CENPB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125817.7.
HGNCiHGNC:1852. CENPB.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4K → Q: Abolishes N-terminal methylation. 1 Publication1

Organism-specific databases

DisGeNETi1059.
OpenTargetsiENSG00000125817.
PharmGKBiPA26397.

Polymorphism and mutation databases

BioMutaiCENPB.
DMDMi116109.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001261252 – 599Major centromere autoantigen BAdd BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylglycine; alternate1 Publication1
Modified residuei2N,N-dimethylglycine; alternate1 Publication1
Modified residuei2N-methylglycine; alternate1 Publication1
Modified residuei156PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Cross-linki246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei396PhosphothreonineCombined sources1
Modified residuei398PhosphothreonineCombined sources1

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.By similarity
N-terminally methylated by METTL11A/NTM1. Alpha-N-methylation is stimulated in response to extracellular stimuli, including increased cell density and heat shock, and seems to facilitate binding to CENP-B boxes. Chromatin-bound CENP-B is primarily trimethylated.1 Publication

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP07199.
MaxQBiP07199.
PaxDbiP07199.
PeptideAtlasiP07199.
PRIDEiP07199.

PTM databases

iPTMnetiP07199.
PhosphoSitePlusiP07199.

Expressioni

Developmental stagei

Expression varies across the cell cycle, with high levels in G2 phase (at the mRNA level).1 Publication

Gene expression databases

BgeeiENSG00000125817.
CleanExiHS_CENPB.
GenevisibleiP07199. HS.

Organism-specific databases

HPAiCAB011626.
HPA053507.
HPA054405.

Interactioni

Subunit structurei

Antiparallel homodimer (PubMed:1469042, PubMed:14522975). Interacts with CENPT (PubMed:19412974). Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292).4 Publications

Protein-protein interaction databases

BioGridi107488. 71 interactors.
CORUMiP07199.
IntActiP07199. 32 interactors.
MINTiMINT-2857560.
STRINGi9606.ENSP00000369075.

Structurei

Secondary structure

1599
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi10 – 22Combined sources13
Beta strandi24 – 26Combined sources3
Helixi28 – 35Combined sources8
Helixi39 – 47Combined sources9
Helixi49 – 59Combined sources11
Helixi60 – 64Combined sources5
Helixi75 – 88Combined sources14
Helixi89 – 91Combined sources3
Helixi97 – 111Combined sources15
Helixi120 – 129Combined sources10
Helixi544 – 558Combined sources15
Helixi565 – 584Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BW6NMR-A1-56[»]
1HLVX-ray2.50A1-129[»]
1UFIX-ray1.65A/B/C/D540-599[»]
ProteinModelPortaliP07199.
SMRiP07199.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07199.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 52HTH psq-typePROSITE-ProRule annotationAdd BLAST51
Domaini65 – 136HTH CENPB-typePROSITE-ProRule annotationAdd BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni536 – 599HomodimerizationAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi404 – 465Glu-rich (acidic)Add BLAST62
Compositional biasi508 – 538Asp/Glu-rich (acidic)Add BLAST31

Phylogenomic databases

eggNOGiKOG3105. Eukaryota.
ENOG4110CDI. LUCA.
GeneTreeiENSGT00760000119149.
HOGENOMiHOG000111537.
HOVERGENiHBG050890.
InParanoidiP07199.
KOiK11496.
OMAiKRRQLTF.
OrthoDBiEOG091G0G48.
PhylomeDBiP07199.
TreeFamiTF101131.

Family and domain databases

Gene3Di1.10.287.1090. 1 hit.
2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR033062. CENP-B.
IPR015115. CenpB_C.
IPR004875. DDE_SF_endonuclease_dom.
IPR034882. Dimerisation_CENP-B_sf.
IPR009057. Homeobox-like_sf.
IPR006600. HTH_CenpB_DNA-bd_dom.
IPR007889. HTH_Psq.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
PANTHERiPTHR45553. PTHR45553. 1 hit.
PfamiView protein in Pfam
PF09026. CENP-B_dimeris. 1 hit.
PF04218. CENP-B_N. 1 hit.
PF03184. DDE_1. 1 hit.
PF03221. HTH_Tnp_Tc5. 1 hit.
SMARTiView protein in SMART
SM00674. CENPB. 1 hit.
SUPFAMiSSF101160. SSF101160. 1 hit.
SSF46689. SSF46689. 2 hits.
PROSITEiView protein in PROSITE
PS51253. HTH_CENPB. 1 hit.
PS50960. HTH_PSQ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR
60 70 80 90 100
AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL
110 120 130 140 150
KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCSGVAR ARARNAAPRT
160 170 180 190 200
PAAPASPAAV PSEGSGGSTT GWRAREEQPP SVAEGYASQD VFSATETSLW
210 220 230 240 250
YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP
260 270 280 290 300
RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA
310 320 330 340 350
AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE
360 370 380 390 400
GQDPSGLQLG LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS
410 420 430 440 450
LKSEGEEEEE EEEEEEEEEG EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE
460 470 480 490 500
EGDVDSDEEE EEDEESSSEG LEAEDWAQGV VEAGGSFGAY GAQEEAQCPT
510 520 530 540 550
LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP VPSFGEAMAY
560 570 580 590
FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
Length:599
Mass (Da):65,171
Last modified:August 1, 1992 - v2
Checksum:i9B4B7DB957A914AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti583R → M in CAA28918 (PubMed:2435739).Curated1
Sequence conflicti592 – 593VR → LL in CAA28918 (PubMed:2435739).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55039 Genomic DNA. Translation: CAA38879.1.
AL109804 Genomic DNA. No translation available.
BC012297 mRNA. Translation: AAH12297.1.
BC053847 mRNA. Translation: AAH53847.1.
X05299 mRNA. Translation: CAA28918.1.
CCDSiCCDS13064.1.
PIRiS18735.
RefSeqiNP_001801.1. NM_001810.5.
UniGeneiHs.516855.

Genome annotation databases

EnsembliENST00000379751; ENSP00000369075; ENSG00000125817.
GeneIDi1059.
KEGGihsa:1059.
UCSCiuc002wjk.3. human.

Similar proteinsi

Entry informationi

Entry nameiCENPB_HUMAN
AccessioniPrimary (citable) accession number: P07199
Secondary accession number(s): Q96EI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: August 1, 1992
Last modified: November 22, 2017
This is version 192 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references