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Protein

L-lactate dehydrogenase B chain

Gene

LDHB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A-like 6A (LDHAL6A), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase, L-lactate dehydrogenase A-like 6B (LDHAL6B), L-lactate dehydrogenase (HEL-S-133P), L-lactate dehydrogenase (LDHAL6A), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHAL6A), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase C chain (LDHC)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NAD1 Publication1
Binding sitei107Substrate1
Binding sitei139NAD or substrate1 Publication1
Binding sitei170Substrate1
Active sitei194Proton acceptor1
Binding sitei249Substrate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 53NADBy similarityAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.27. 2681.
ReactomeiR-HSA-70268. Pyruvate metabolism.
SABIO-RKiP07195.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Alternative name(s):
LDH heart subunit
Short name:
LDH-H
Renal carcinoma antigen NY-REN-46
Gene namesi
Name:LDHB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:6541. LDHB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: HPA
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • membrane raft Source: UniProtKB
  • mitochondrion Source: Ensembl
  • myelin sheath Source: Ensembl

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Lactate dehydrogenase B deficiency (LDHBD)9 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA condition with no deleterious effects on health. LDHBD is of interest to laboratory medicine mainly because it can cause misdiagnosis in those disorders in which elevation of serum LDH is expected.
See also OMIM:614128
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0041737K → E in LDHBD; slightly decreased activity. 1 PublicationCorresponds to variant dbSNP:rs118203897Ensembl.1
Natural variantiVAR_00417435A → E in LDHBD. 1 Publication1
Natural variantiVAR_01163469G → E in LDHBD. 1 Publication1
Natural variantiVAR_011635107R → W in LDHBD; inactive. 1 PublicationCorresponds to variant dbSNP:rs777954556Ensembl.1
Natural variantiVAR_004175129S → R in LDHBD. 1 PublicationCorresponds to variant dbSNP:rs118203896Ensembl.1
Natural variantiVAR_004176171F → V in LDHBD. 1 Publication1
Natural variantiVAR_004177172R → H in LDHBD. 2 PublicationsCorresponds to variant dbSNP:rs118203895Ensembl.1
Natural variantiVAR_011636172R → P in LDHBD. 1 Publication1
Natural variantiVAR_004178175M → L in LDHBD. 1 Publication1
Natural variantiVAR_011637223Missing in LDHBD. 1 Publication1
Natural variantiVAR_004179322D → V in LDHBD. 1 Publication1
Natural variantiVAR_011638325W → R in LDHBD. Corresponds to variant dbSNP:rs267607212Ensembl.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi3945.
MalaCardsiLDHB.
MIMi614128. phenotype.
OpenTargetsiENSG00000111716.
Orphaneti284435. Glycogen storage disease due to lactate dehydrogenase H-subunit deficiency.
PharmGKBiPA30325.

Chemistry databases

ChEMBLiCHEMBL4940.
DrugBankiDB00157. NADH.
DB03940. Oxamic Acid.

Polymorphism and mutation databases

BioMutaiLDHB.
DMDMi126041.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684592 – 334L-lactate dehydrogenase B chainAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei5N6-acetyllysineBy similarity1
Modified residuei7N6-acetyllysineCombined sources1
Modified residuei18PhosphothreonineBy similarity1
Modified residuei44PhosphoserineCombined sources1
Modified residuei58N6-acetyllysineCombined sources1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei119N6-acetyllysineCombined sources1
Modified residuei127N6-acetyllysineBy similarity1
Modified residuei240PhosphotyrosineCombined sources1
Modified residuei319N6-acetyllysineBy similarity1
Modified residuei329N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP07195.
PaxDbiP07195.
PeptideAtlasiP07195.
PRIDEiP07195.
TopDownProteomicsiP07195.

2D gel databases

DOSAC-COBS-2DPAGEiP07195.
OGPiP07195.
REPRODUCTION-2DPAGEiIPI00219217.
SWISS-2DPAGEiP07195.
UCD-2DPAGEiP07195.

PTM databases

iPTMnetiP07195.
PhosphoSitePlusiP07195.
SwissPalmiP07195.

Expressioni

Gene expression databases

BgeeiENSG00000111716.
CleanExiHS_LDHB.
ExpressionAtlasiP07195. baseline and differential.
GenevisibleiP07195. HS.

Organism-specific databases

HPAiCAB004641.
HPA019007.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • kinase binding Source: Ensembl

Protein-protein interaction databases

BioGridi110137. 136 interactors.
IntActiP07195. 29 interactors.
MINTiMINT-1144561.
STRINGi9606.ENSP00000229319.

Chemistry databases

BindingDBiP07195.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Beta strandi9 – 13Combined sources5
Beta strandi21 – 27Combined sources7
Helixi31 – 42Combined sources12
Beta strandi47 – 52Combined sources6
Helixi56 – 68Combined sources13
Helixi69 – 72Combined sources4
Beta strandi76 – 80Combined sources5
Helixi84 – 87Combined sources4
Beta strandi91 – 95Combined sources5
Helixi107 – 110Combined sources4
Helixi111 – 128Combined sources18
Beta strandi133 – 136Combined sources4
Beta strandi138 – 140Combined sources3
Helixi141 – 152Combined sources12
Helixi156 – 158Combined sources3
Beta strandi159 – 161Combined sources3
Helixi165 – 179Combined sources15
Helixi183 – 185Combined sources3
Beta strandi190 – 192Combined sources3
Helixi202 – 204Combined sources3
Helixi212 – 215Combined sources4
Turni217 – 220Combined sources4
Beta strandi221 – 223Combined sources3
Beta strandi225 – 227Combined sources3
Helixi229 – 246Combined sources18
Helixi251 – 265Combined sources15
Beta strandi270 – 277Combined sources8
Turni279 – 282Combined sources4
Beta strandi289 – 297Combined sources9
Beta strandi300 – 304Combined sources5
Helixi311 – 328Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I0ZX-ray2.10A/B2-334[»]
1T2FX-ray3.00A/B/C/D2-332[»]
ProteinModelPortaliP07195.
SMRiP07195.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07195.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP07195.
KOiK00016.
OMAiRQPGQTR.
OrthoDBiEOG091G0HME.
PhylomeDBiP07195.
TreeFamiTF314963.

Family and domain databases

HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG
310 320 330
LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Length:334
Mass (Da):36,638
Last modified:January 23, 2007 - v2
Checksum:i3AD605DEED0D54A2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0041737K → E in LDHBD; slightly decreased activity. 1 PublicationCorresponds to variant dbSNP:rs118203897Ensembl.1
Natural variantiVAR_00417435A → E in LDHBD. 1 Publication1
Natural variantiVAR_01163469G → E in LDHBD. 1 Publication1
Natural variantiVAR_011635107R → W in LDHBD; inactive. 1 PublicationCorresponds to variant dbSNP:rs777954556Ensembl.1
Natural variantiVAR_004175129S → R in LDHBD. 1 PublicationCorresponds to variant dbSNP:rs118203896Ensembl.1
Natural variantiVAR_004176171F → V in LDHBD. 1 Publication1
Natural variantiVAR_004177172R → H in LDHBD. 2 PublicationsCorresponds to variant dbSNP:rs118203895Ensembl.1
Natural variantiVAR_011636172R → P in LDHBD. 1 Publication1
Natural variantiVAR_004178175M → L in LDHBD. 1 Publication1
Natural variantiVAR_049758175M → V. Corresponds to variant dbSNP:rs7966339Ensembl.1
Natural variantiVAR_011637223Missing in LDHBD. 1 Publication1
Natural variantiVAR_004179322D → V in LDHBD. 1 Publication1
Natural variantiVAR_011638325W → R in LDHBD. Corresponds to variant dbSNP:rs267607212Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13794
, X13795, X13796, X13797, X13798, X13799, X13800 Genomic DNA. Translation: CAA32033.1.
Y00711 mRNA. Translation: CAA68701.1.
BC002362 mRNA. Translation: AAH02362.1.
BC015122 mRNA. Translation: AAH15122.1.
BC071860 mRNA. Translation: AAH71860.1.
CCDSiCCDS8691.1.
PIRiS02795. DEHULH.
RefSeqiNP_001167568.1. NM_001174097.2.
NP_001302466.1. NM_001315537.1.
NP_002291.1. NM_002300.7.
UniGeneiHs.446149.

Genome annotation databases

EnsembliENST00000350669; ENSP00000229319; ENSG00000111716.
ENST00000396076; ENSP00000379386; ENSG00000111716.
GeneIDi3945.
KEGGihsa:3945.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLDHB_HUMAN
AccessioniPrimary (citable) accession number: P07195
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 206 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  8. SIMILARITY comments
    Index of protein domains and families