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P07173

- CYCR_BLAVI

UniProt

P07173 - CYCR_BLAVI

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Protein

Photosynthetic reaction center cytochrome c subunit

Gene

pufC

Organism
Blastochloris viridis (Rhodopseudomonas viridis)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei21 – 211Not N-palmitoylated
Metal bindingi94 – 941Iron (heme 1 axial ligand)
Binding sitei107 – 1071Heme 1 (covalent)
Binding sitei110 – 1101Heme 1 (covalent)
Metal bindingi111 – 1111Iron (heme 1 axial ligand)
Metal bindingi130 – 1301Iron (heme 2 axial ligand)
Metal bindingi144 – 1441Iron (heme 4 axial ligand)
Binding sitei152 – 1521Heme 2 (covalent)
Binding sitei155 – 1551Heme 2 (covalent)
Metal bindingi156 – 1561Iron (heme 2 axial ligand)
Metal bindingi253 – 2531Iron (heme 3 axial ligand)
Binding sitei264 – 2641Heme 3 (covalent)
Binding sitei267 – 2671Heme 3 (covalent)
Metal bindingi268 – 2681Iron (heme 3 axial ligand)
Binding sitei325 – 3251Heme 4 (covalent)
Binding sitei328 – 3281Heme 4 (covalent)
Metal bindingi329 – 3291Iron (heme 4 axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
  2. photosynthesis, light reaction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Photosynthetic reaction center cytochrome c subunit
Alternative name(s):
Cytochrome c558/c559
Gene namesi
Name:pufC
Synonyms:cytC
OrganismiBlastochloris viridis (Rhodopseudomonas viridis)
Taxonomic identifieri1079 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeBlastochloris

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
  2. plasma membrane light-harvesting complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Reaction center

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 356336Photosynthetic reaction center cytochrome c subunitPRO_0000006551Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi21 – 211S-diacylglycerol cysteine1 PublicationPROSITE-ProRule annotation

Post-translational modificationi

Binds 4 heme groups per subunit.
After the signal sequence is removed, the N-terminal cysteine is modified to form a diacylglyceride thioether, but the alpha-amino group is free and is not N-palmitoylated.

Keywords - PTMi

Lipoprotein

Structurei

Secondary structure

1
356
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi45 – 5511Combined sources
Beta strandi69 – 713Combined sources
Helixi72 – 754Combined sources
Beta strandi76 – 783Combined sources
Beta strandi80 – 823Combined sources
Helixi87 – 10115Combined sources
Turni103 – 1053Combined sources
Helixi106 – 1094Combined sources
Helixi122 – 14019Combined sources
Helixi142 – 1454Combined sources
Turni146 – 1483Combined sources
Helixi152 – 1565Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi172 – 1754Combined sources
Turni182 – 1843Combined sources
Helixi189 – 1913Combined sources
Helixi192 – 1976Combined sources
Turni198 – 2025Combined sources
Helixi209 – 2135Combined sources
Beta strandi215 – 2173Combined sources
Beta strandi226 – 2294Combined sources
Beta strandi234 – 2363Combined sources
Helixi237 – 2393Combined sources
Helixi244 – 26017Combined sources
Helixi264 – 2663Combined sources
Helixi270 – 2723Combined sources
Helixi282 – 30019Combined sources
Helixi303 – 3075Combined sources
Helixi311 – 3133Combined sources
Helixi325 – 3295Combined sources
Beta strandi332 – 3343Combined sources
Helixi335 – 3384Combined sources
Helixi342 – 3443Combined sources
Helixi346 – 3483Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DXRX-ray2.00C21-352[»]
1PRCX-ray2.30C21-356[»]
1R2CX-ray2.86C21-356[»]
1VRNX-ray2.20C21-352[»]
2I5NX-ray1.96C21-356[»]
2JBLX-ray2.40C1-356[»]
2PRCX-ray2.45C21-356[»]
2WJMX-ray1.95C21-356[»]
2WJNX-ray1.86C21-356[»]
2X5UX-ray3.00C21-356[»]
2X5VX-ray3.00C21-356[»]
3D38X-ray3.21C21-356[»]
3G7FX-ray2.50C21-356[»]
3PRCX-ray2.40C21-356[»]
3T6DX-ray1.95C1-356[»]
3T6EX-ray1.92C1-356[»]
4AC5X-ray8.20C21-356[»]
4CASX-ray3.50A1-356[»]
5PRCX-ray2.35C21-356[»]
6PRCX-ray2.30C21-356[»]
7PRCX-ray2.65C21-356[»]
ProteinModelPortaliP07173.
SMRiP07173. Positions 21-352.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07173.

Family & Domainsi

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di1.10.468.10. 2 hits.
InterProiIPR011031. Multihaem_cyt.
IPR023119. Multihaem_cyt_PRC_cyt_su-like.
IPR003158. Photosyn_RC_cyt_c-su.
[Graphical view]
PfamiPF02276. CytoC_RC. 1 hit.
[Graphical view]
PIRSFiPIRSF000017. RC_cytochrome. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07173-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQLIVNSVA TVALASLVAG CFEPPPATTT QTGFRGLSMG EVLHPATVKA
60 70 80 90 100
KKERDAQYPP ALAAVKAEGP PVSQVYKNVK VLGNLTEAEF LRTMTAITEW
110 120 130 140 150
VSPQEGCTYC HDENNLASEA KYPYVVARRM LEMTRAINTN WTQHVAQTGV
160 170 180 190 200
TCYTCHRGTP LPPYVRYLEP TLPLNNRETP THVERVETRS GYVVRLAKYT
210 220 230 240 250
AYSALNYDPF TMFLANDKRQ VRVVPQTALP LVGVSRGKER RPLSDAYATF
260 270 280 290 300
ALMMSISDSL GTNCTFCHNA QTFESWGKKS TPQRAIAWWG IRMVRDLNMN
310 320 330 340 350
YLAPLNASLP ASRLGRQGEA PQADCRTCHQ GVTKPLFGAS RLKDYPELGP

IKAAAK
Length:356
Mass (Da):39,371
Last modified:April 1, 1988 - v1
Checksum:iECE3D64F1BB0877A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05768 Genomic DNA. Translation: CAA29223.1.
M16317 Genomic DNA. Translation: AAA26093.1.
PIRiS00139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05768 Genomic DNA. Translation: CAA29223.1 .
M16317 Genomic DNA. Translation: AAA26093.1 .
PIRi S00139.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DXR X-ray 2.00 C 21-352 [» ]
1PRC X-ray 2.30 C 21-356 [» ]
1R2C X-ray 2.86 C 21-356 [» ]
1VRN X-ray 2.20 C 21-352 [» ]
2I5N X-ray 1.96 C 21-356 [» ]
2JBL X-ray 2.40 C 1-356 [» ]
2PRC X-ray 2.45 C 21-356 [» ]
2WJM X-ray 1.95 C 21-356 [» ]
2WJN X-ray 1.86 C 21-356 [» ]
2X5U X-ray 3.00 C 21-356 [» ]
2X5V X-ray 3.00 C 21-356 [» ]
3D38 X-ray 3.21 C 21-356 [» ]
3G7F X-ray 2.50 C 21-356 [» ]
3PRC X-ray 2.40 C 21-356 [» ]
3T6D X-ray 1.95 C 1-356 [» ]
3T6E X-ray 1.92 C 1-356 [» ]
4AC5 X-ray 8.20 C 21-356 [» ]
4CAS X-ray 3.50 A 1-356 [» ]
5PRC X-ray 2.35 C 21-356 [» ]
6PRC X-ray 2.30 C 21-356 [» ]
7PRC X-ray 2.65 C 21-356 [» ]
ProteinModelPortali P07173.
SMRi P07173. Positions 21-352.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P07173.

Family and domain databases

Gene3Di 1.10.468.10. 2 hits.
InterProi IPR011031. Multihaem_cyt.
IPR023119. Multihaem_cyt_PRC_cyt_su-like.
IPR003158. Photosyn_RC_cyt_c-su.
[Graphical view ]
Pfami PF02276. CytoC_RC. 1 hit.
[Graphical view ]
PIRSFi PIRSF000017. RC_cytochrome. 1 hit.
PROSITEi PS51008. MULTIHEME_CYTC. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence of the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis."
    Weyer K.A., Lottspeich F., Lang F., Oesterhelt D., Michel H.
    EMBO J. 6:2197-2202(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE.
    Strain: ATCC 19567 / DSM 133 / F.
  2. "The cytochrome subunit of the photosynthetic reaction center from Rhodopseudomonas viridis is a lipoprotein."
    Weyer K.A., Schaefer W., Lottspeich F., Michel H.
    Biochemistry 26:2909-2914(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-46, DIACYLGLYCEROL AT CYS-21.
  3. "Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3-A resolution."
    Deisenhofer J., Epp O., Miki K., Huber R., Michel H.
    Nature 318:618-624(1985)
    Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
  4. "X-ray structure analysis of a membrane protein complex. Electron density map at 3-A resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis."
    Deisenhofer J., Epp O., Miki K., Huber R., Michel H.
    J. Mol. Biol. 180:385-398(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
  5. "The coupling of light-induced electron transfer and proton uptake as derived from crystal structures of reaction centres from Rhodopseudomonas viridis modified at the binding site of the secondary quinone, QB."
    Lancaster C.R.D., Michel H.
    Structure 5:1339-1359(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).
    Strain: ATCC 19567 / DSM 133 / F.
  6. "Refined crystal structures of reaction centres from Rhodopseudomonas viridis in complexes with the herbicide atrazine and two chiral atrazine derivatives also lead to a new model of the bound carotenoid."
    Lancaster C.R.D., Michel H.
    J. Mol. Biol. 286:883-898(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
    Strain: ATCC 19567 / DSM 133 / F.

Entry informationi

Entry nameiCYCR_BLAVI
AccessioniPrimary (citable) accession number: P07173
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3