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P07173

- CYCR_BLAVI

UniProt

P07173 - CYCR_BLAVI

Protein

Photosynthetic reaction center cytochrome c subunit

Gene

pufC

Organism
Blastochloris viridis (Rhodopseudomonas viridis)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei21 – 211Not N-palmitoylated
    Metal bindingi94 – 941Iron (heme 1 axial ligand)
    Binding sitei107 – 1071Heme 1 (covalent)
    Binding sitei110 – 1101Heme 1 (covalent)
    Metal bindingi111 – 1111Iron (heme 1 axial ligand)
    Metal bindingi130 – 1301Iron (heme 2 axial ligand)
    Metal bindingi144 – 1441Iron (heme 4 axial ligand)
    Binding sitei152 – 1521Heme 2 (covalent)
    Binding sitei155 – 1551Heme 2 (covalent)
    Metal bindingi156 – 1561Iron (heme 2 axial ligand)
    Metal bindingi253 – 2531Iron (heme 3 axial ligand)
    Binding sitei264 – 2641Heme 3 (covalent)
    Binding sitei267 – 2671Heme 3 (covalent)
    Metal bindingi268 – 2681Iron (heme 3 axial ligand)
    Binding sitei325 – 3251Heme 4 (covalent)
    Binding sitei328 – 3281Heme 4 (covalent)
    Metal bindingi329 – 3291Iron (heme 4 axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW
    2. photosynthesis, light reaction Source: InterPro

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Photosynthetic reaction center cytochrome c subunit
    Alternative name(s):
    Cytochrome c558/c559
    Gene namesi
    Name:pufC
    Synonyms:cytC
    OrganismiBlastochloris viridis (Rhodopseudomonas viridis)
    Taxonomic identifieri1079 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaeBlastochloris

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell
    2. plasma membrane light-harvesting complex Source: InterPro

    Keywords - Cellular componenti

    Cell membrane, Membrane, Reaction center

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 356336Photosynthetic reaction center cytochrome c subunitPRO_0000006551Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi21 – 211S-diacylglycerol cysteine1 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    Binds 4 heme groups per subunit.
    After the signal sequence is removed, the N-terminal cysteine is modified to form a diacylglyceride thioether, but the alpha-amino group is free and is not N-palmitoylated.

    Keywords - PTMi

    Lipoprotein

    Structurei

    Secondary structure

    1
    356
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi45 – 5511
    Beta strandi69 – 713
    Helixi72 – 754
    Beta strandi76 – 783
    Beta strandi80 – 823
    Helixi87 – 10115
    Turni103 – 1053
    Helixi106 – 1094
    Helixi122 – 14019
    Helixi142 – 1454
    Turni146 – 1483
    Helixi152 – 1565
    Beta strandi159 – 1613
    Beta strandi172 – 1754
    Turni182 – 1843
    Helixi189 – 1913
    Helixi192 – 1976
    Turni198 – 2025
    Helixi209 – 2135
    Beta strandi215 – 2173
    Beta strandi226 – 2294
    Beta strandi234 – 2363
    Helixi237 – 2393
    Helixi244 – 26017
    Helixi264 – 2663
    Helixi270 – 2723
    Helixi282 – 30019
    Helixi303 – 3075
    Helixi311 – 3133
    Helixi325 – 3295
    Beta strandi332 – 3343
    Helixi335 – 3384
    Helixi342 – 3443
    Helixi346 – 3483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DXRX-ray2.00C21-352[»]
    1PRCX-ray2.30C21-356[»]
    1R2CX-ray2.86C21-356[»]
    1VRNX-ray2.20C21-352[»]
    2I5NX-ray1.96C21-356[»]
    2JBLX-ray2.40C1-356[»]
    2PRCX-ray2.45C21-356[»]
    2WJMX-ray1.95C21-356[»]
    2WJNX-ray1.86C21-356[»]
    2X5UX-ray3.00C21-356[»]
    2X5VX-ray3.00C21-356[»]
    3D38X-ray3.21C21-356[»]
    3G7FX-ray2.50C21-356[»]
    3PRCX-ray2.40C21-356[»]
    3T6DX-ray1.95C1-356[»]
    3T6EX-ray1.92C1-356[»]
    4AC5X-ray8.20C21-356[»]
    4CASX-ray3.50A1-356[»]
    5PRCX-ray2.35C21-356[»]
    6PRCX-ray2.30C21-356[»]
    7PRCX-ray2.65C21-356[»]
    ProteinModelPortaliP07173.
    SMRiP07173. Positions 21-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP07173.

    Family & Domainsi

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    Gene3Di1.10.468.10. 2 hits.
    InterProiIPR011031. Multihaem_cyt.
    IPR023119. Multihaem_cyt_PRC_cyt_su-like.
    IPR003158. Photosyn_RC_cyt_c-su.
    [Graphical view]
    PfamiPF02276. CytoC_RC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000017. RC_cytochrome. 1 hit.
    PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07173-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQLIVNSVA TVALASLVAG CFEPPPATTT QTGFRGLSMG EVLHPATVKA    50
    KKERDAQYPP ALAAVKAEGP PVSQVYKNVK VLGNLTEAEF LRTMTAITEW 100
    VSPQEGCTYC HDENNLASEA KYPYVVARRM LEMTRAINTN WTQHVAQTGV 150
    TCYTCHRGTP LPPYVRYLEP TLPLNNRETP THVERVETRS GYVVRLAKYT 200
    AYSALNYDPF TMFLANDKRQ VRVVPQTALP LVGVSRGKER RPLSDAYATF 250
    ALMMSISDSL GTNCTFCHNA QTFESWGKKS TPQRAIAWWG IRMVRDLNMN 300
    YLAPLNASLP ASRLGRQGEA PQADCRTCHQ GVTKPLFGAS RLKDYPELGP 350
    IKAAAK 356
    Length:356
    Mass (Da):39,371
    Last modified:April 1, 1988 - v1
    Checksum:iECE3D64F1BB0877A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05768 Genomic DNA. Translation: CAA29223.1.
    M16317 Genomic DNA. Translation: AAA26093.1.
    PIRiS00139.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05768 Genomic DNA. Translation: CAA29223.1 .
    M16317 Genomic DNA. Translation: AAA26093.1 .
    PIRi S00139.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DXR X-ray 2.00 C 21-352 [» ]
    1PRC X-ray 2.30 C 21-356 [» ]
    1R2C X-ray 2.86 C 21-356 [» ]
    1VRN X-ray 2.20 C 21-352 [» ]
    2I5N X-ray 1.96 C 21-356 [» ]
    2JBL X-ray 2.40 C 1-356 [» ]
    2PRC X-ray 2.45 C 21-356 [» ]
    2WJM X-ray 1.95 C 21-356 [» ]
    2WJN X-ray 1.86 C 21-356 [» ]
    2X5U X-ray 3.00 C 21-356 [» ]
    2X5V X-ray 3.00 C 21-356 [» ]
    3D38 X-ray 3.21 C 21-356 [» ]
    3G7F X-ray 2.50 C 21-356 [» ]
    3PRC X-ray 2.40 C 21-356 [» ]
    3T6D X-ray 1.95 C 1-356 [» ]
    3T6E X-ray 1.92 C 1-356 [» ]
    4AC5 X-ray 8.20 C 21-356 [» ]
    4CAS X-ray 3.50 A 1-356 [» ]
    5PRC X-ray 2.35 C 21-356 [» ]
    6PRC X-ray 2.30 C 21-356 [» ]
    7PRC X-ray 2.65 C 21-356 [» ]
    ProteinModelPortali P07173.
    SMRi P07173. Positions 21-352.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P07173.

    Family and domain databases

    Gene3Di 1.10.468.10. 2 hits.
    InterProi IPR011031. Multihaem_cyt.
    IPR023119. Multihaem_cyt_PRC_cyt_su-like.
    IPR003158. Photosyn_RC_cyt_c-su.
    [Graphical view ]
    Pfami PF02276. CytoC_RC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000017. RC_cytochrome. 1 hit.
    PROSITEi PS51008. MULTIHEME_CYTC. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis."
      Weyer K.A., Lottspeich F., Lang F., Oesterhelt D., Michel H.
      EMBO J. 6:2197-2202(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE.
      Strain: ATCC 19567 / DSM 133 / F.
    2. "The cytochrome subunit of the photosynthetic reaction center from Rhodopseudomonas viridis is a lipoprotein."
      Weyer K.A., Schaefer W., Lottspeich F., Michel H.
      Biochemistry 26:2909-2914(1987)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-46, DIACYLGLYCEROL AT CYS-21.
    3. "Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3-A resolution."
      Deisenhofer J., Epp O., Miki K., Huber R., Michel H.
      Nature 318:618-624(1985)
      Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
    4. "X-ray structure analysis of a membrane protein complex. Electron density map at 3-A resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis."
      Deisenhofer J., Epp O., Miki K., Huber R., Michel H.
      J. Mol. Biol. 180:385-398(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3 ANGSTROMS).
    5. "The coupling of light-induced electron transfer and proton uptake as derived from crystal structures of reaction centres from Rhodopseudomonas viridis modified at the binding site of the secondary quinone, QB."
      Lancaster C.R.D., Michel H.
      Structure 5:1339-1359(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).
      Strain: ATCC 19567 / DSM 133 / F.
    6. "Refined crystal structures of reaction centres from Rhodopseudomonas viridis in complexes with the herbicide atrazine and two chiral atrazine derivatives also lead to a new model of the bound carotenoid."
      Lancaster C.R.D., Michel H.
      J. Mol. Biol. 286:883-898(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
      Strain: ATCC 19567 / DSM 133 / F.

    Entry informationi

    Entry nameiCYCR_BLAVI
    AccessioniPrimary (citable) accession number: P07173
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3