P07172 (HIS8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidinol-phosphate aminotransferase
EC=2.6.1.9

Alternative name(s):
Imidazole acetol-phosphate transaminase

Gene names

Name:	HIS5
Ordered Locus Names:	YIL116W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	intracellular Traceable author statement. Source: SGD
Molecular function	L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC histidinol-phosphate transaminase activity Inferred from mutant phenotype. Source: SGD pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Histidinol-phosphate aminotransferase		PRO_0000153509
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine Probable
Experimental info
	Sequence conflict	110 – 112	3	PGK → RE in CAA29139. Ref.1
	Sequence conflict	125	1	Y → S in CAA29139. Ref.1
	Sequence conflict	142	1	T → I in CAA29139. Ref.1
	Sequence conflict	294	1	R → L in CAA29139. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07172 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: E026E09DB0443C45	FASTA	385	42,646
	10 20 30 40 50 60 MVFDLKRIVR PKIYNLEPYR CARDDFTEGI LLDANENAHG PTPVELSKTN LHRYPDPHQL 70 80 90 100 110 120 EFKTAMTKYR NKTSSYANDP EVKPLTADNL CLGVGSDESI DAIIRACCVP GKEKILVLPP 130 140 150 160 170 180 TYSMYSVCAN INDIEVVQCP LTVSDGSFQM DTEAVLTILK NDSLIKLMFV TSPGNPTGAK 190 200 210 220 230 240 IKTSLIEKVL QNWDNGLVVV DEAYVDFCGG STAPLVTKYP NLVTLQTLSK SFGLAGIRLG 250 260 270 280 290 300 MTYATAELAR ILNAMKAPYN ISSLASEYAL KAVQDSNLKK MEATSKIINE EKMRLLKELT 310 320 330 340 350 360 ALDYVDDQYV GGLDANFLLI RINGGDNVLA KKLYYQLATQ SGVVVRFRGN ELGCSGCLRI 370 380 TVGTHEENTH LIKYFKETLY KLANE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the yeast HIS5 gene responsive to general control of amino acid biosynthesis." Nishiwaki K., Hayashi N., Irie S., Chung D.-H., Harashima S., Oshima Y. Mol. Gen. Genet. 208:159-167(1987) [PubMed: 3302607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Studies on the HIS5 gene of yeast: the nucleotide sequence of 5' upstream region of the HIS5 gene of Saccharomyces cerevisiae." Chung D.H., Nishiwaki K., Oshima Y. Sanop Misaengmul Hakhoe Chi 13:19-25(1985) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38125 Genomic DNA. Translation: CAA86264.1. X05650 Genomic DNA. Translation: CAA29139.1. M38613 Genomic DNA. Translation: AAA34675.1. BK006942 Genomic DNA. Translation: DAA08437.1.
PIR	S48456.
RefSeq	NP_012150.1. NM_001179464.1.
3D structure databases
ProteinModelPortal	P07172.
SMR	P07172. Positions 2-377.
ModBase	Search...
Protein-protein interaction databases
STRING	P07172.
Proteomic databases
PeptideAtlas	P07172.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL116W; YIL116W; YIL116W.
GeneID	854690.
KEGG	sce:YIL116W.
NMPDR	fig|4932.3.peg.1674.
Organism-specific databases
CYGD	YIL116w.
SGD	S000001378. HIS5.
Phylogenomic databases
eggNOG	fuNOG07719.
GeneTree	EFGT00050000001336.
HOGENOM	HBG646350.
OMA	DKIVDCP.
OrthoDB	EOG4H75M3.
Gene expression databases
ArrayExpress	P07172.
Genevestigator	P07172.
GermOnline	YIL116W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR005861. HisP_aminotrans. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00817.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01141. HisC. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977316.

Entry information

Entry name

HIS8_YEAST

Accession

Primary (citable) accession number: P07172
Secondary accession number(s): D6VVH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program