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Protein

Histidinol-phosphate aminotransferase

Gene

HIS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate.

Cofactori

Pathwayi: L-histidine biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (HIS1)
  2. Histidine biosynthesis trifunctional protein (HIS4)
  3. Histidine biosynthesis trifunctional protein (HIS4)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6)
  5. Imidazole glycerol phosphate synthase hisHF (HIS7)
  6. Imidazoleglycerol-phosphate dehydratase (HIS3)
  7. Histidinol-phosphate aminotransferase (HIS5)
  8. Histidinol-phosphatase (HIS2)
  9. Histidine biosynthesis trifunctional protein (HIS4)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • histidine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciYEAST:YIL116W-MONOMER.
UniPathwayiUPA00031; UER00012.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol-phosphate aminotransferase (EC:2.6.1.9)
Alternative name(s):
Imidazole acetol-phosphate transaminase
Gene namesi
Name:HIS5
Ordered Locus Names:YIL116W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL116W.
SGDiS000001378. HIS5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Histidinol-phosphate aminotransferasePRO_0000153509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301N6-(pyridoxal phosphate)lysineCurated

Proteomic databases

MaxQBiP07172.
TopDownProteomicsiP07172.

Interactioni

Protein-protein interaction databases

BioGridi34875. 10 interactions.
MINTiMINT-4490038.

Structurei

3D structure databases

ProteinModelPortaliP07172.
SMRiP07172. Positions 22-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000288512.
InParanoidiP07172.
KOiK00817.
OMAiIKPPYNI.
OrthoDBiEOG7ZGXCZ.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFDLKRIVR PKIYNLEPYR CARDDFTEGI LLDANENAHG PTPVELSKTN
60 70 80 90 100
LHRYPDPHQL EFKTAMTKYR NKTSSYANDP EVKPLTADNL CLGVGSDESI
110 120 130 140 150
DAIIRACCVP GKEKILVLPP TYSMYSVCAN INDIEVVQCP LTVSDGSFQM
160 170 180 190 200
DTEAVLTILK NDSLIKLMFV TSPGNPTGAK IKTSLIEKVL QNWDNGLVVV
210 220 230 240 250
DEAYVDFCGG STAPLVTKYP NLVTLQTLSK SFGLAGIRLG MTYATAELAR
260 270 280 290 300
ILNAMKAPYN ISSLASEYAL KAVQDSNLKK MEATSKIINE EKMRLLKELT
310 320 330 340 350
ALDYVDDQYV GGLDANFLLI RINGGDNVLA KKLYYQLATQ SGVVVRFRGN
360 370 380
ELGCSGCLRI TVGTHEENTH LIKYFKETLY KLANE
Length:385
Mass (Da):42,646
Last modified:February 1, 1995 - v2
Checksum:iE026E09DB0443C45
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1123PGK → RE in CAA29139 (PubMed:3302607).Curated
Sequence conflicti125 – 1251Y → S in CAA29139 (PubMed:3302607).Curated
Sequence conflicti142 – 1421T → I in CAA29139 (PubMed:3302607).Curated
Sequence conflicti294 – 2941R → L in CAA29139 (PubMed:3302607).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86264.1.
X05650 Genomic DNA. Translation: CAA29139.1.
M38613 Genomic DNA. Translation: AAA34675.1.
BK006942 Genomic DNA. Translation: DAA08437.1.
PIRiS48456.
RefSeqiNP_012150.1. NM_001179464.1.

Genome annotation databases

EnsemblFungiiYIL116W; YIL116W; YIL116W.
GeneIDi854690.
KEGGisce:YIL116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86264.1.
X05650 Genomic DNA. Translation: CAA29139.1.
M38613 Genomic DNA. Translation: AAA34675.1.
BK006942 Genomic DNA. Translation: DAA08437.1.
PIRiS48456.
RefSeqiNP_012150.1. NM_001179464.1.

3D structure databases

ProteinModelPortaliP07172.
SMRiP07172. Positions 22-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34875. 10 interactions.
MINTiMINT-4490038.

Proteomic databases

MaxQBiP07172.
TopDownProteomicsiP07172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL116W; YIL116W; YIL116W.
GeneIDi854690.
KEGGisce:YIL116W.

Organism-specific databases

EuPathDBiFungiDB:YIL116W.
SGDiS000001378. HIS5.

Phylogenomic databases

HOGENOMiHOG000288512.
InParanoidiP07172.
KOiK00817.
OMAiIKPPYNI.
OrthoDBiEOG7ZGXCZ.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00012.
BioCyciYEAST:YIL116W-MONOMER.

Miscellaneous databases

PROiP07172.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01023. HisC_aminotrans_2.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR005861. HisP_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01141. hisC. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the yeast HIS5 gene responsive to general control of amino acid biosynthesis."
    Nishiwaki K., Hayashi N., Irie S., Chung D.-H., Harashima S., Oshima Y.
    Mol. Gen. Genet. 208:159-167(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Studies on the HIS5 gene of yeast: the nucleotide sequence of 5' upstream region of the HIS5 gene of Saccharomyces cerevisiae."
    Chung D.H., Nishiwaki K., Oshima Y.
    Sanop Misaengmul Hakhoe Chi 13:19-25(1985)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.

Entry informationi

Entry nameiHIS8_YEAST
AccessioniPrimary (citable) accession number: P07172
Secondary accession number(s): D6VVH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.