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Protein

Calbindin

Gene

Calb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi24 – 351Add BLAST12
Calcium bindingi111 – 1222Add BLAST12
Calcium bindingi155 – 1663Add BLAST12
Calcium bindingi199 – 2104Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • vitamin D binding Source: UniProtKB-KW
  • zinc ion binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding, Vitamin D

Names & Taxonomyi

Protein namesi
Recommended name:
Calbindin
Alternative name(s):
Calbindin D28
D-28K
Spot 35 protein
Vitamin D-dependent calcium-binding protein, avian-type
Gene namesi
Name:Calb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi69340. Calb1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: BHF-UCL
  • cytoplasm Source: RGD
  • cytosol Source: GO_Central
  • dendrite Source: Ensembl
  • extracellular exosome Source: Ensembl
  • neuronal cell body Source: Ensembl
  • nucleus Source: RGD
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000734752 – 261CalbindinAdd BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP07171.
PRIDEiP07171.

PTM databases

iPTMnetiP07171.
PhosphoSitePlusiP07171.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007456.
GenevisibleiP07171. RN.

Interactioni

Subunit structurei

Interacts with RANBP9.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010845.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 5Combined sources4
Turni6 – 8Combined sources3
Helixi13 – 23Combined sources11
Beta strandi28 – 31Combined sources4
Helixi34 – 50Combined sources17
Helixi56 – 65Combined sources10
Helixi68 – 70Combined sources3
Helixi75 – 81Combined sources7
Helixi88 – 92Combined sources5
Helixi93 – 95Combined sources3
Helixi100 – 107Combined sources8
Turni112 – 114Combined sources3
Beta strandi116 – 118Combined sources3
Helixi120 – 134Combined sources15
Helixi140 – 153Combined sources14
Beta strandi156 – 160Combined sources5
Helixi164 – 170Combined sources7
Turni173 – 175Combined sources3
Helixi178 – 183Combined sources6
Helixi188 – 198Combined sources11
Beta strandi201 – 204Combined sources4
Helixi208 – 221Combined sources14
Turni223 – 225Combined sources3
Turni228 – 230Combined sources3
Helixi231 – 239Combined sources9
Helixi249 – 251Combined sources3
Helixi253 – 256Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F33NMR-A1-261[»]
2G9BNMR-A1-261[»]
ProteinModelPortaliP07171.
SMRiP07171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07171.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 46EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini53 – 88EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini98 – 133EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini142 – 177EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Domaini186 – 221EF-hand 5PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 7Interaction with RANBP9By similarity6

Domaini

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

Sequence similaritiesi

Belongs to the calbindin family.Curated
Contains 5 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP07171.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG091G0GNH.
PhylomeDBiP07171.
TreeFamiTF325083.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELLQARKKA
60 70 80 90 100
GLELSPEMKT FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC
110 120 130 140 150
EEFMKTWRKY DTDHSGFIET EELKNFLKDL LEKANKTVDD TKLAEYTDLM
160 170 180 190 200
LKLFDSNNDG KLELTEMARL LPVQENFLLK FQGIKMCGKE FNKAFELYDQ
210 220 230 240 250
DGNGYIDENE LDALLKDLCE KNKQELDINN ISTYKKNIMA LSDGGKLYRT
260
DLALILSAGD N
Length:261
Mass (Da):29,994
Last modified:January 23, 2007 - v2
Checksum:iB9C58D75C4D252C1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139D → H in AAA40852 (PubMed:2792772).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31178 mRNA. Translation: AAA40851.1.
X04280 mRNA. Translation: CAA27828.1.
M27839 Genomic DNA. Translation: AAA40852.1.
BC081764 mRNA. Translation: AAH81764.1.
PIRiA30808. KLRTB.
RefSeqiNP_114190.1. NM_031984.2.
UniGeneiRn.3908.

Genome annotation databases

EnsembliENSRNOT00000010845; ENSRNOP00000010845; ENSRNOG00000007456.
GeneIDi83839.
KEGGirno:83839.
UCSCiRGD:69340. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31178 mRNA. Translation: AAA40851.1.
X04280 mRNA. Translation: CAA27828.1.
M27839 Genomic DNA. Translation: AAA40852.1.
BC081764 mRNA. Translation: AAH81764.1.
PIRiA30808. KLRTB.
RefSeqiNP_114190.1. NM_031984.2.
UniGeneiRn.3908.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2F33NMR-A1-261[»]
2G9BNMR-A1-261[»]
ProteinModelPortaliP07171.
SMRiP07171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010845.

PTM databases

iPTMnetiP07171.
PhosphoSitePlusiP07171.

Proteomic databases

PaxDbiP07171.
PRIDEiP07171.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010845; ENSRNOP00000010845; ENSRNOG00000007456.
GeneIDi83839.
KEGGirno:83839.
UCSCiRGD:69340. rat.

Organism-specific databases

CTDi793.
RGDi69340. Calb1.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00390000013118.
HOGENOMiHOG000231866.
HOVERGENiHBG000855.
InParanoidiP07171.
KOiK14757.
OMAiFFRCQQL.
OrthoDBiEOG091G0GNH.
PhylomeDBiP07171.
TreeFamiTF325083.

Miscellaneous databases

EvolutionaryTraceiP07171.
PROiP07171.

Gene expression databases

BgeeiENSRNOG00000007456.
GenevisibleiP07171. RN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR029634. Calbindin.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR19972:SF3. PTHR19972:SF3. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALB1_RAT
AccessioniPrimary (citable) accession number: P07171
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.