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P07164 (AEQ1_AEQVI) Reviewed, UniProtKB/Swiss-Prot

Last modified September 18, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aequorin-1
OrganismAequorea victoria (Jellyfish)
Taxonomic identifier6100 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaHydrozoaHydroidaLeptomedusaeAequoreidaeAequorea

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ca2+-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO2 with the concomitant emission of light.

Post-translational modification

The reduction of the disulfide bond is necessary to regenerate aequorin from apoaequorin.

Biotechnological use

Aequorin is used as an intracellular Ca2+ indicator. Aequorin has a number of advantages over other Ca2+ indicators, for example, low leakage rate from cells, lack of intracellular compartmentalization or sequestration and it does not disrupt cell functions or embryo development.

Sequence similarities

Belongs to the aequorin family.

Contains 4 EF-hand domains.

Caution

Was originally (Ref.4) thought to have a internal disulfide bond.

Ontologies

Keywords
   Biological processLuminescence
   DomainRepeat
   LigandCalcium
Metal-binding
   Molecular functionPhotoprotein
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processbioluminescence

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 77
PRO_0000004126
Chain8 – 196189Aequorin-1
PRO_0000004127

Regions

Domain18 – 5336EF-hand 1
Domain54 – 10855EF-hand 2
Domain111 – 14636EF-hand 3
Domain147 – 18236EF-hand 4
Calcium binding31 – 42121
Calcium binding124 – 135122
Calcium binding160 – 171123
Region47 – 5711May interact with the chromophore
Region62 – 7211May interact with the chromophore
Region107 – 11711May interact with the chromophore

Experimental info

Mutagenesis1961Missing: Loss of bioluminescence. Ref.3

Secondary structure

......................... 196
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07164 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 9AA5B636288A5B8F

FASTA19622,514
        10         20         30         40         50         60 
MTSEQYSVKL TPDFDNPKWI GRHKHMFNFL DVNHNGRISL DEMVYKASDI VINNLGATPE 

        70         80         90        100        110        120 
QAKRHKDAVE AFFGGAGMKY GVETEWPEYI EGWKRLASEE LKRYSKNQIT LIRLWGDALF 

       130        140        150        160        170        180 
DIIDKDQNGA ISLDEWKAYT KSDGIIQSSE DCEETFRVCD IDESGQLDVD EMTRQHLGFW 

       190 
YTMDPACEKL YGGAVP 

« Hide

References

[1]"Sequence comparisons of complementary DNAs encoding aequorin isotypes."
Prasher D.C., McCann R.O., Longiaru M., Cormier M.J.
Biochemistry 26:1326-1332(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Amino acid sequence of the calcium-dependent photoprotein aequorin."
Charbonneau H., Walsh K.A., McCann R.O., Prendergast F.G., Cormier M.J., Vanaman T.C.
Biochemistry 24:6762-6771(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 8-196.
[3]"A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin."
Nomura M., Inouye S., Ohmiya Y., Tsuji F.I.
FEBS Lett. 295:63-66(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF PRO-196.
[4]"Mass spectrometric evidence for a disulfide bond in aequorin regeneration."
Ohmiya Y., Kurono S., Ohashi M., Fagan T.F., Tsuji F.I.
FEBS Lett. 332:226-228(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY DISULFIDE BOND.
[5]"All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin."
Deng L., Vysotski E.S., Markova S.V., Liu Z.-J., Lee J., Rose J., Wang B.-C.
Protein Sci. 14:663-675(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 7-196 IN COMPLEX WITH CALCIUM IONS.

Web resources

Wikipedia

Aequorin entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M16103 mRNA. Translation: AAA27716.1.
PIRA26623.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SL8X-ray1.70A7-196[»]
ProteinModelPortalP07164.
SMRP07164. Positions 16-196.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA1.13.12.5. 8923.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 3 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07164.

Entry information

Entry nameAEQ1_AEQVI
AccessionPrimary (citable) accession number: P07164
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: September 18, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references