Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

Rpn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • peptide transport Source: RGD
  • protein N-linked glycosylation via asparagine Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Ribophorin I
Short name:
RPN-I
Ribophorin-1
Gene namesi
Name:Rpn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3594. Rpn1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 437LumenalSequence analysisAdd BLAST415
Transmembranei438 – 455HelicalSequence analysisAdd BLAST18
Topological domaini456 – 605CytoplasmicSequence analysisAdd BLAST150

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000002224323 – 605Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1Add BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei185N6-acetyllysineBy similarity1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei536N6-acetyllysine; alternateBy similarity1
Cross-linki536Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP07153.
PeptideAtlasiP07153.
PRIDEiP07153.

PTM databases

iPTMnetiP07153.
PhosphoSitePlusiP07153.
SwissPalmiP07153.

Expressioni

Tissue specificityi

Expressed in all tissues tested.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).By similarity

Protein-protein interaction databases

BioGridi247626. 4 interactors.
IntActiP07153. 2 interactors.
MINTiMINT-4996362.
STRINGi10116.ENSRNOP00000066002.

Structurei

3D structure databases

SMRiP07153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
HOVERGENiHBG012864.
InParanoidiP07153.

Family and domain databases

InterProiView protein in InterPro
IPR007676. Ribophorin_I.
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiView protein in Pfam
PF04597. Ribophorin_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPIVLLLL LWLALAPTPG SASSEAPPLV NEDVKRTVDL SSHLAKVTAE
60 70 80 90 100
VVLAHPGGGS TARASSFVLA LEPELESRLA HLGVQVKGED EEDNNLEVRE
110 120 130 140 150
TKMKGKSGRF FTVKLPVALD PGSKISIVVE TVYTHVLHPY PTQITQSEKQ
160 170 180 190 200
FVVFEGNHYF YSPYPTKTQT MRVRLASRNV ESHTKLGNPS RSEDILDYGP
210 220 230 240 250
FKDIPAYSQD TFKVHYENNS PFLTITSMTR VIEVSHWGNI AVEENVDLKH
260 270 280 290 300
TGAVLKGPFS RYDYQRQPDS GISSIRSFKT ILPAAAQDVY YRDEIGNVST
310 320 330 340 350
SHLLILDDSV EMEIRPRFGL FGGWKTHYIV GYNLPSYEYL YNLGDQYALK
360 370 380 390 400
MRFVDHVFDE QVIDSLTVKI ILPEGAKNIQ VDSPYDISRA PDELHYTYLD
410 420 430 440 450
TFGRPVIVAY KKNLVEQHIQ DIVVHYTFNK VLMLQEPLLV VAAFYILFFT
460 470 480 490 500
VIIYVRLDFS ITKDPAAEAR MKVACITEQV LTLVNKRLGL YRHFDETVNR
510 520 530 540 550
YKQSRDISTL NSGKKSLETE HKAVTSEIAV LQSRLKTEGS DLCDRVSEMQ
560 570 580 590 600
KLDAQVKELV LKSAVEAERL VAGKLKKDTY IENEKLSSGK RQELVTKIDH

ILDAL
Length:605
Mass (Da):68,304
Last modified:April 1, 1988 - v1
Checksum:i60F8ECAAF9C4806F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16 – 31APTPG…PPLVN → PDAWQRLFGGSAAGQR (PubMed:2335524).CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05300 mRNA. Translation: CAA28919.1.
M33508 Genomic DNA. Translation: AAA42043.1.
PIRiA27274.
UniGeneiRn.233900.
Rn.4224.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRPN1_RAT
AccessioniPrimary (citable) accession number: P07153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 10, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families