P07153 (RPN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
EC=2.4.1.119

Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Ribophorin I
Short name=RPN-I
Ribophorin-1

Gene names

Name:

Rpn1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome By similarity.
Tissue specificity	Expressed in all tissues tested.
Sequence similarities	Belongs to the OST1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Acetylation Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptide transport Non-traceable author statement Ref.1. Source: RGD protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell rough microsome Inferred from direct assay. Source: RGD
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 605	583	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1		PRO_0000022243
Regions
	Topological domain	23 – 437	415	Lumenal Potential
	Transmembrane	438 – 455	18	Helical; Potential
	Topological domain	456 – 605	150	Cytoplasmic Potential
Amino acid modifications
	Modified residue	185	1	N6-acetyllysine By similarity
	Glycosylation	297	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	16 – 31	16	APTPG…PPLVN → PDAWQRLFGGSAAGQR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07153 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 60F8ECAAF9C4806F	FASTA	605	68,304
	10 20 30 40 50 60 MEAPIVLLLL LWLALAPTPG SASSEAPPLV NEDVKRTVDL SSHLAKVTAE VVLAHPGGGS 70 80 90 100 110 120 TARASSFVLA LEPELESRLA HLGVQVKGED EEDNNLEVRE TKMKGKSGRF FTVKLPVALD 130 140 150 160 170 180 PGSKISIVVE TVYTHVLHPY PTQITQSEKQ FVVFEGNHYF YSPYPTKTQT MRVRLASRNV 190 200 210 220 230 240 ESHTKLGNPS RSEDILDYGP FKDIPAYSQD TFKVHYENNS PFLTITSMTR VIEVSHWGNI 250 260 270 280 290 300 AVEENVDLKH TGAVLKGPFS RYDYQRQPDS GISSIRSFKT ILPAAAQDVY YRDEIGNVST 310 320 330 340 350 360 SHLLILDDSV EMEIRPRFGL FGGWKTHYIV GYNLPSYEYL YNLGDQYALK MRFVDHVFDE 370 380 390 400 410 420 QVIDSLTVKI ILPEGAKNIQ VDSPYDISRA PDELHYTYLD TFGRPVIVAY KKNLVEQHIQ 430 440 450 460 470 480 DIVVHYTFNK VLMLQEPLLV VAAFYILFFT VIIYVRLDFS ITKDPAAEAR MKVACITEQV 490 500 510 520 530 540 LTLVNKRLGL YRHFDETVNR YKQSRDISTL NSGKKSLETE HKAVTSEIAV LQSRLKTEGS 550 560 570 580 590 600 DLCDRVSEMQ KLDAQVKELV LKSAVEAERL VAGKLKKDTY IENEKLSSGK RQELVTKIDH ILDAL

References

[1]	"Isolation and characterization of cDNA clones for rat ribophorin I: complete coding sequence and in vitro synthesis and insertion of the encoded product into endoplasmic reticulum membranes." Harnik-Ort V., Prakash K., Marcantonio E., Colman D.R., Rosenfeld M.G., Adesnik M., Sabatini D.D., Kreibich G. J. Cell Biol. 104:855-863(1987) [PubMed: 3031084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Structure and chromosomal location of the rat ribophorin I gene." Behal A., Prakash K., D'Eustachio P., Adesnik M., Sabatini D.D., Kreibich G. J. Biol. Chem. 265:8252-8258(1990) [PubMed: 2335524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05300 mRNA. Translation: CAA28919.1. M33508 Genomic DNA. Translation: AAA42043.1.
IPI	IPI00204365.
PIR	A27274.
RefSeq	NP_037199.1. NM_013067.1.
UniGene	Rn.4224.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P07153. 1 interaction.
MINT	MINT-4996362.
PTM databases
PhosphoSite	P07153.
Proteomic databases
PRIDE	P07153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25596.
KEGG	rno:25596.
Organism-specific databases
CTD	6184.
RGD	3594. Rpn1.
Phylogenomic databases
HOVERGEN	HBG012864.
Gene expression databases
Genevestigator	P07153.
Family and domain databases
InterPro	IPR007676. Ribophorin_I. [Graphical view]
KO	K12666.
PANTHER	PTHR21049. Ribophorin_I. 1 hit.
Pfam	PF04597. Ribophorin_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607287.

Entry information

Entry name

RPN1_RAT

Accession

Primary (citable) accession number: P07153

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program