Reviewed,
UniProtKB/Swiss-Prot P07152 (MMP10_RAT)
Last modified
June 16, 2009.
Version 92.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Stromelysin-2 Short name=SL-2 EC=3.4.24.22 Alternative name(s): Matrix metalloproteinase-10 Short name=MMP-10 Transin-2 Transformation-associated protein 34A | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 476 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. |
| Catalytic activity | Similar to stromelysin 1, but action on collagen types III, IV and V is weak. |
| Cofactor | Binds 2 zinc ions per subunit By similarity. Calcium By similarity. |
| Subcellular location | Secreted › extracellular space › extracellular matrix Probable. |
| Domain | The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. |
| Sequence similarities | Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Collagen degradation |
| Cellular component | Extracellular matrix Secreted |
| Domain | Repeat Signal |
| Ligand | Calcium Metal-binding Zinc |
| Molecular function | Hydrolase Metalloprotease Protease |
| PTM | Disulfide bond Zymogen |
| Gene Ontology (GO) | |
| Biological process | collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell proteinaceous extracellular matrixInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||
Molecule processing | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | Probable | |||||||
| Propeptide | 18 – 99 | 82 | Activation peptide By similarity | PRO_0000028768 | ||||||
| Chain | 100 – 476 | 377 | Stromelysin-2 | PRO_0000028769 | ||||||
Regions | ||||||||||
| Domain | 295 – 337 | 43 | Hemopexin-like 1 | |||||||
| Domain | 339 – 382 | 44 | Hemopexin-like 2 | |||||||
| Domain | 387 – 434 | 48 | Hemopexin-like 3 | |||||||
| Domain | 436 – 476 | 41 | Hemopexin-like 4 | |||||||
| Motif | 90 – 97 | 8 | Cysteine switch By similarity | |||||||
Sites | ||||||||||
| Active site | 219 | 1 | By similarity | |||||||
| Metal binding | 92 | 1 | Zinc; in inhibited form By similarity | |||||||
| Metal binding | 218 | 1 | Zinc; catalytic By similarity | |||||||
| Metal binding | 222 | 1 | Zinc; catalytic By similarity | |||||||
| Metal binding | 228 | 1 | Zinc; catalytic By similarity | |||||||
Amino acid modifications | ||||||||||
| Disulfide bond | 289 ↔ 476 | By similarity | ||||||||
Sequences
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References
| [1] | "Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene." Breathnach R., Matrisian L.M., Gesnel M.-C., Staub A., Leroy P. Nucleic Acids Res. 15:1139-1151(1987) [PubMed: 3547333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Molecular cloning and characterization of v-mos-activated transformation-associated proteins." Chan J.C., Scanlon M., Zhang H.Z., Jia L.B., Yu D., Hung M.C., French M., Eastman E.M. J. Biol. Chem. 267:1099-1103(1992) [PubMed: 1370458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| X05083 mRNA. Translation: CAA28739.1. M65253 mRNA. Translation: AAA42202.1. | |
| IPI | IPI00204362. |
| PIR | KCRTS2. B26403. |
| RefSeq | NP_598198.1. |
| UniGene | Rn.9946 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HY7 based on UniProtKB P08254. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | M10.011. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000032832. Rattus norvegicus. [Contig view] |
| GeneID | 117061. |
| KEGG | rno:117061. |
Organism-specific databases | |
| RGD | 620192. Mmp10. |
Phylogenomic databases | |
| HOVERGEN | P07152. |
Enzyme and pathway databases | |
| BRENDA | 3.4.24.22. 248. |
Gene expression databases | |
| ArrayExpress | P07152. |
| GermOnline | ENSRNOG00000032832. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view] |
| Gene3D | G3DSA:2.110.10.10. Hemopexin. 1 hit. |
| Pfam | PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF001191. Peptidase_M10A_matrix. 1 hit. |
| PRINTS | PR00138. MATRIXIN. |
| SMART | SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view] |
| PROSITE | PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 619922. |
Entry information
| Entry name | MMP10_RAT | ||||||||
| Accession | Primary (citable) accession number: P07152 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


