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P07152 (MMP10_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Stromelysin-2
Short name=SL-2
EC=3.4.24.22
Alternative name(s):
Matrix metalloproteinase-10
Short name=MMP-10
Transformation-associated protein 34A
Transin-2
Gene names
Name:Mmp10
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

Catalytic activity

Similar to stromelysin 1, but action on collagen types III, IV and V is weak.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Calcium By similarity.

Subcellular location

Secreted › extracellular space › extracellular matrix Probable.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similarities

Belongs to the peptidase M10A family.

Contains 4 hemopexin-like domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Probable
Propeptide18 – 9982Activation peptide By similarity
PRO_0000028768
Chain100 – 476377Stromelysin-2
PRO_0000028769

Regions

Domain295 – 33743Hemopexin-like 1
Domain339 – 38244Hemopexin-like 2
Domain387 – 43448Hemopexin-like 3
Domain436 – 47641Hemopexin-like 4
Motif90 – 978Cysteine switch By similarity

Sites

Active site2191 By similarity
Metal binding921Zinc; in inhibited form By similarity
Metal binding1681Zinc 1 By similarity
Metal binding1701Zinc 1 By similarity
Metal binding1831Zinc 1 By similarity
Metal binding1961Zinc 1 By similarity
Metal binding2181Zinc 2; catalytic By similarity
Metal binding2221Zinc 2; catalytic By similarity
Metal binding2281Zinc 2; catalytic By similarity

Amino acid modifications

Disulfide bond289 ↔ 476 By similarity

Sequences

Sequence LengthMass (Da)Tools
P07152 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: B556B6FB1D8BA7EE

FASTA47654,222
        10         20         30         40         50         60 
MEPLAILVLL CFPICSAYPL HGAVRQDHST MDLAQQYLEK YYNFRKNEKQ FFKRKDSSPV 

        70         80         90        100        110        120 
VKKIEEMQKF LGLEMTGKLD SNTVEMMHKP RCGVPDVGGF STFPGSPKWR KNHISYRIVN 

       130        140        150        160        170        180 
YTLDLPRESV DSAIERALKV WEEVTPLTFS RISEGEADIM ISFAVGEHGD FYPFDGVGQS 

       190        200        210        220        230        240 
LAHAYPPGPG FYGDAHFDDD EKWSLGPSGT NLFLVAAHEL GHSLGLFHSN NKESLMYPVY 

       250        260        270        280        290        300 
RFSTSQANIR LSQDDIEGIQ SLYGARPSSD ATVVPVPSVS PKPETPVKCD PALSFDAVTM 

       310        320        330        340        350        360 
LRGEFLFFKD RHFWRRTQWN PEPEFHLISA FWPSLPSGLD AAYEANNKDR VLIFKGSQFW 

       370        380        390        400        410        420 
AVRGNEVQAG YPKRIHTLGF PPTVKKIDAA VFEKEKKKTY FFVGDKYWRF DETRQLMDKG 

       430        440        450        460        470 
FPRLITDDFP GIEPQVDAVL HAFGFFYFFC GSSQFEFDPN ARTVTHTLKS NSWLLC

« Hide

References

[1]"Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene."
Breathnach R., Matrisian L.M., Gesnel M.-C., Staub A., Leroy P.
Nucleic Acids Res. 15:1139-1151(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning and characterization of v-mos-activated transformation-associated proteins."
Chan J.C., Scanlon M., Zhang H.Z., Jia L.B., Yu D., Hung M.C., French M., Eastman E.M.
J. Biol. Chem. 267:1099-1103(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X05083 mRNA. Translation: CAA28739.1.
M65253 mRNA. Translation: AAA42202.1.
IPIIPI00204362.
PIRKCRTS2. B26403.
RefSeqNP_598198.1. NM_133514.1.
UniGeneRn.9946.

3D structure databases

ProteinModelPortalP07152.
SMRP07152. Positions 33-267.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013118.

Protein family/group databases

MEROPSM10.011.

Proteomic databases

PaxDbP07152.
PRIDEP07152.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID117061.
KEGGrno:117061.
UCSCRGD:620192. rat.

Organism-specific databases

CTD4319.
RGD620192. Mmp10.

Phylogenomic databases

eggNOGNOG270148.
HOGENOMHOG000217927.
HOVERGENHBG052484.
InParanoidP07152.
KOK01396.
OrthoDBEOG4KKZ2X.

Gene expression databases

GenevestigatorP07152.
GermOnlineENSRNOG00000032832. Rattus norvegicus.

Family and domain databases

Gene3D2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_Metazoans.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSPR00138. MATRIXIN.
SMARTSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMSSF50923. Hemopexin. 1 hit.
SSF47090. PGBD_like. 1 hit.
PROSITEPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619922.

Entry information

Entry nameMMP10_RAT
AccessionPrimary (citable) accession number: P07152
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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