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P07152

- MMP10_RAT

UniProt

P07152 - MMP10_RAT

Protein

Stromelysin-2

Gene

Mmp10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

    Catalytic activityi

    Similar to stromelysin 1, but action on collagen types III, IV and V is weak.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity
    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921Zinc; in inhibited formBy similarity
    Metal bindingi168 – 1681Zinc 1By similarity
    Metal bindingi170 – 1701Zinc 1By similarity
    Metal bindingi183 – 1831Zinc 1By similarity
    Metal bindingi196 – 1961Zinc 1By similarity
    Metal bindingi218 – 2181Zinc 2; catalyticBy similarity
    Active sitei219 – 2191PROSITE-ProRule annotation
    Metal bindingi222 – 2221Zinc 2; catalyticBy similarity
    Metal bindingi228 – 2281Zinc 2; catalyticBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. metalloendopeptidase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. collagen catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    Collagen degradation

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM10.011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Stromelysin-2 (EC:3.4.24.22)
    Short name:
    SL-2
    Alternative name(s):
    Matrix metalloproteinase-10
    Short name:
    MMP-10
    Transformation-associated protein 34A
    Transin-2
    Gene namesi
    Name:Mmp10
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620192. Mmp10.

    Subcellular locationi

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717CuratedAdd
    BLAST
    Propeptidei18 – 9982Activation peptideBy similarityPRO_0000028768Add
    BLAST
    Chaini100 – 476377Stromelysin-2PRO_0000028769Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi289 ↔ 476By similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP07152.
    PRIDEiP07152.

    Expressioni

    Gene expression databases

    GenevestigatoriP07152.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000013118.

    Structurei

    3D structure databases

    ProteinModelPortaliP07152.
    SMRiP07152. Positions 33-267.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati286 – 33550Hemopexin 1Add
    BLAST
    Repeati336 – 38247Hemopexin 2Add
    BLAST
    Repeati384 – 43249Hemopexin 3Add
    BLAST
    Repeati433 – 47644Hemopexin 4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi90 – 978Cysteine switchBy similarity

    Domaini

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Belongs to the peptidase M10A family.Curated
    Contains 4 hemopexin repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG270148.
    HOGENOMiHOG000217927.
    HOVERGENiHBG052484.
    InParanoidiP07152.
    KOiK01396.
    PhylomeDBiP07152.

    Family and domain databases

    Gene3Di2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR018486. Hemopexin_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR021158. Pept_M10A_Zn_BS.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PfamiPF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSiPR00138. MATRIXIN.
    SMARTiSM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47090. SSF47090. 1 hit.
    SSF50923. SSF50923. 1 hit.
    PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
    PS00024. HEMOPEXIN. 1 hit.
    PS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07152-1 [UniParc]FASTAAdd to Basket

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    MEPLAILVLL CFPICSAYPL HGAVRQDHST MDLAQQYLEK YYNFRKNEKQ    50
    FFKRKDSSPV VKKIEEMQKF LGLEMTGKLD SNTVEMMHKP RCGVPDVGGF 100
    STFPGSPKWR KNHISYRIVN YTLDLPRESV DSAIERALKV WEEVTPLTFS 150
    RISEGEADIM ISFAVGEHGD FYPFDGVGQS LAHAYPPGPG FYGDAHFDDD 200
    EKWSLGPSGT NLFLVAAHEL GHSLGLFHSN NKESLMYPVY RFSTSQANIR 250
    LSQDDIEGIQ SLYGARPSSD ATVVPVPSVS PKPETPVKCD PALSFDAVTM 300
    LRGEFLFFKD RHFWRRTQWN PEPEFHLISA FWPSLPSGLD AAYEANNKDR 350
    VLIFKGSQFW AVRGNEVQAG YPKRIHTLGF PPTVKKIDAA VFEKEKKKTY 400
    FFVGDKYWRF DETRQLMDKG FPRLITDDFP GIEPQVDAVL HAFGFFYFFC 450
    GSSQFEFDPN ARTVTHTLKS NSWLLC 476
    Length:476
    Mass (Da):54,222
    Last modified:April 1, 1988 - v1
    Checksum:iB556B6FB1D8BA7EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05083 mRNA. Translation: CAA28739.1.
    M65253 mRNA. Translation: AAA42202.1.
    PIRiB26403. KCRTS2.
    RefSeqiNP_598198.1. NM_133514.1.
    UniGeneiRn.9946.

    Genome annotation databases

    GeneIDi117061.
    KEGGirno:117061.
    UCSCiRGD:620192. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05083 mRNA. Translation: CAA28739.1 .
    M65253 mRNA. Translation: AAA42202.1 .
    PIRi B26403. KCRTS2.
    RefSeqi NP_598198.1. NM_133514.1.
    UniGenei Rn.9946.

    3D structure databases

    ProteinModelPortali P07152.
    SMRi P07152. Positions 33-267.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000013118.

    Protein family/group databases

    MEROPSi M10.011.

    Proteomic databases

    PaxDbi P07152.
    PRIDEi P07152.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 117061.
    KEGGi rno:117061.
    UCSCi RGD:620192. rat.

    Organism-specific databases

    CTDi 4319.
    RGDi 620192. Mmp10.

    Phylogenomic databases

    eggNOGi NOG270148.
    HOGENOMi HOG000217927.
    HOVERGENi HBG052484.
    InParanoidi P07152.
    KOi K01396.
    PhylomeDBi P07152.

    Miscellaneous databases

    NextBioi 619922.

    Gene expression databases

    Genevestigatori P07152.

    Family and domain databases

    Gene3Di 2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR018486. Hemopexin_CS.
    IPR024079. MetalloPept_cat_dom.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR021158. Pept_M10A_Zn_BS.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    Pfami PF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSi PR00138. MATRIXIN.
    SMARTi SM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47090. SSF47090. 1 hit.
    SSF50923. SSF50923. 1 hit.
    PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
    PS00024. HEMOPEXIN. 1 hit.
    PS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene."
      Breathnach R., Matrisian L.M., Gesnel M.-C., Staub A., Leroy P.
      Nucleic Acids Res. 15:1139-1151(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning and characterization of v-mos-activated transformation-associated proteins."
      Chan J.C., Scanlon M., Zhang H.Z., Jia L.B., Yu D., Hung M.C., French M., Eastman E.M.
      J. Biol. Chem. 267:1099-1103(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiMMP10_RAT
    AccessioniPrimary (citable) accession number: P07152
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3