Reviewed, UniProtKB/Swiss-Prot P07152 (MMP10_RAT)

Names and origin

Protein names

Recommended name:
    Stromelysin-2
      Short name=SL-2
    EC=3.4.24.22
Alternative name(s):
    Matrix metalloproteinase-10
      Short name=MMP-10
    Transin-2
    Transformation-associated protein 34A

Gene names

Name:

Mmp10

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Catalytic activity	Similar to stromelysin 1, but action on collagen types III, IV and V is weak.
Cofactor	Binds 2 zinc ions per subunit By similarity. Calcium By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix Probable.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Biological process	Collagen degradation
Cellular component	Extracellular matrix Secreted
Domain	Repeat Signal
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Probable
	Propeptide	18 – 99	82	Activation peptide By similarity		PRO_0000028768
	Chain	100 – 476	377	Stromelysin-2		PRO_0000028769
Regions
	Domain	295 – 337	43	Hemopexin-like 1
	Domain	339 – 382	44	Hemopexin-like 2
	Domain	387 – 434	48	Hemopexin-like 3
	Domain	436 – 476	41	Hemopexin-like 4
	Motif	90 – 97	8	Cysteine switch By similarity
Sites
	Active site	219	1	By similarity
	Metal binding	92	1	Zinc; in inhibited form By similarity
	Metal binding	218	1	Zinc; catalytic By similarity
	Metal binding	222	1	Zinc; catalytic By similarity
	Metal binding	228	1	Zinc; catalytic By similarity
Amino acid modifications
	Disulfide bond	289 ↔ 476		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07152-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: B556B6FB1D8BA7EE	FASTA	476	54,222
	10 20 30 40 50 60 MEPLAILVLL CFPICSAYPL HGAVRQDHST MDLAQQYLEK YYNFRKNEKQ FFKRKDSSPV 70 80 90 100 110 120 VKKIEEMQKF LGLEMTGKLD SNTVEMMHKP RCGVPDVGGF STFPGSPKWR KNHISYRIVN 130 140 150 160 170 180 YTLDLPRESV DSAIERALKV WEEVTPLTFS RISEGEADIM ISFAVGEHGD FYPFDGVGQS 190 200 210 220 230 240 LAHAYPPGPG FYGDAHFDDD EKWSLGPSGT NLFLVAAHEL GHSLGLFHSN NKESLMYPVY 250 260 270 280 290 300 RFSTSQANIR LSQDDIEGIQ SLYGARPSSD ATVVPVPSVS PKPETPVKCD PALSFDAVTM 310 320 330 340 350 360 LRGEFLFFKD RHFWRRTQWN PEPEFHLISA FWPSLPSGLD AAYEANNKDR VLIFKGSQFW 370 380 390 400 410 420 AVRGNEVQAG YPKRIHTLGF PPTVKKIDAA VFEKEKKKTY FFVGDKYWRF DETRQLMDKG 430 440 450 460 470 FPRLITDDFP GIEPQVDAVL HAFGFFYFFC GSSQFEFDPN ARTVTHTLKS NSWLLC

References

[1]	"Sequences coding for part of oncogene-induced transin are highly conserved in a related rat gene." Breathnach R., Matrisian L.M., Gesnel M.-C., Staub A., Leroy P. Nucleic Acids Res. 15:1139-1151(1987) [PubMed: 3547333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and characterization of v-mos-activated transformation-associated proteins." Chan J.C., Scanlon M., Zhang H.Z., Jia L.B., Yu D., Hung M.C., French M., Eastman E.M. J. Biol. Chem. 267:1099-1103(1992) [PubMed: 1370458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05083 mRNA. Translation: CAA28739.1. M65253 mRNA. Translation: AAA42202.1.
IPI	IPI00204362.
PIR	KCRTS2. B26403.
RefSeq	NP_598198.1.
UniGene	Rn.9946
3D structure databases
HSSP	HSSP built from PDB template 1HY7 based on UniProtKB P08254.
ModBase	Search...
Protein family/group databases
MEROPS	M10.011.
Genome annotation databases
Ensembl	ENSRNOG00000032832. Rattus norvegicus. [Contig view]
GeneID	117061.
KEGG	rno:117061.
Organism-specific databases
RGD	620192. Mmp10.
Phylogenomic databases
HOVERGEN	P07152.
Enzyme and pathway databases
BRENDA	3.4.24.22. 248.
Gene expression databases
ArrayExpress	P07152.
GermOnline	ENSRNOG00000032832. Rattus norvegicus.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 1 hit.
Pfam	PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	619922.

Entry information

Entry name

MMP10_RAT

Accession

Primary (citable) accession number: P07152

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)