P07150 (ANXA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A1

Alternative name(s):
Annexin I
Annexin-1
Calpactin II
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 inhibitory protein
p35

Gene names

Name:	Anxa1
Synonyms:	Anx1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Subunit structure	Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cell projection › cilium By similarity. Basolateral cell membrane By similarity. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium By similarity. Found in the cytoplasm of type II pneumocytes and alveolar macrophages By similarity.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification	Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity By similarity.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cilium Cytoplasm Membrane Nucleus
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
Molecular function	Phospholipase A2 inhibitor
PTM	Acetylation Isopeptide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	alpha-beta T cell differentiation Inferred from electronic annotation. Source: Compara arachidonic acid secretion Inferred from electronic annotation. Source: Compara cell cycle Inferred from electronic annotation. Source: Compara cell surface receptor signaling pathway Inferred from direct assay PubMed 12459455. Source: RGD cellular response to glucocorticoid stimulus Inferred from direct assay PubMed 2936963. Source: BHF-UCL cellular response to hydrogen peroxide Inferred from mutant phenotype PubMed 8607818. Source: RGD endocrine pancreas development Inferred from expression pattern PubMed 7527053. Source: RGD estrous cycle phase Inferred from expression pattern PubMed 17283243. Source: RGD gliogenesis Inferred from expression pattern PubMed 9263577. Source: RGD hepatocyte differentiation Inferred from expression pattern PubMed 11668598. Source: RGD insulin secretion Inferred from direct assay PubMed 12459455. Source: RGD keratinocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of acute inflammatory response Inferred from direct assay PubMed 17917587. Source: RGD negative regulation of apoptotic process Inferred from direct assay PubMed 17917587. Source: RGD negative regulation of protein secretion Inferred from mutant phenotype PubMed 8559285. Source: RGD neutrophil clearance Inferred from electronic annotation. Source: Compara peptide cross-linking Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from mutant phenotype PubMed 8607818. Source: RGD positive regulation of neutrophil apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of prostaglandin biosynthetic process Inferred from mutant phenotype PubMed 8260938. Source: RGD positive regulation of vesicle fusion Inferred from electronic annotation. Source: Compara regulation of cell proliferation Inferred from electronic annotation. Source: Compara response to X-ray Inferred from expression pattern PubMed 9269316. Source: RGD response to drug Inferred from expression pattern PubMed 9416328. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 17283243. Source: RGD response to interleukin-1 Inferred from expression pattern PubMed 11311405. Source: RGD response to peptide hormone stimulus Inferred from expression pattern PubMed 8828496. Source: RGD
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cilium Inferred from electronic annotation. Source: UniProtKB-SubCell cornified envelope Inferred from electronic annotation. Source: Compara extracellular space Inferred from direct assay PubMed 10489933. Source: RGD mitochondrial membrane Inferred from direct assay PubMed 10806526. Source: RGD nucleus Inferred from direct assay PubMed 17994624. Source: RGD plasma membrane Inferred from direct assay PubMed 12467520. Source: RGD protein complex Inferred from direct assay PubMed 10548514. Source: RGD sarcolemma Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Traceable author statement PubMed 12459455. Source: RGD phospholipase A2 inhibitor activity Inferred from direct assay PubMed 10806526. Source: RGD protein homodimerization activity Inferred from direct assay PubMed 8335093. Source: RGD structural molecule activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 346	345	Annexin A1		PRO_0000067464
Regions
	Repeat	51 – 111	61	Annexin 1
	Repeat	123 – 183	61	Annexin 2
	Repeat	207 – 267	61	Annexin 3
	Repeat	282 – 342	61	Annexin 4
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.5
	Modified residue	5	1	Phosphoserine; by TRPM7 By similarity
	Modified residue	21	1	Phosphotyrosine; by EGFR By similarity
	Modified residue	27	1	Phosphoserine; by PKC By similarity
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	312	1	N6-acetyllysine By similarity
	Cross-link	19		Isoglutamyl lysine isopeptide (Gln-Lys) (interchain with K-?) By similarity
Experimental info
	Sequence conflict	322	1	P → S in AAB19866. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P07150 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F046E5F410AF2CF5	FASTA	346	38,829
	10 20 30 40 50 60 MAMVSEFLKQ ACYIEKQEQE YVQAVKSYKG GPGSAVSPYP SFNPSSDVAA LHKAIMVKGV 70 80 90 100 110 120 DEATIIDILT KRTNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA MLKTPAQFDA 130 140 150 160 170 180 DELRAAMKGL GTDEDTLIEI LTTRSNQQIR EITRVYREEL KRDLAKDITS DTSGDFRNAL 190 200 210 220 230 240 LALAKGDRCE DMSVNQDLAD TDARALYEAG ERRKGTDVNV FNTILTTRSY PHLRKVFQNY 250 260 270 280 290 300 RKYSQHDMNK ALDLELKGDI EKCLTTIVKC ATSTPAFFAE KLYEAMKGAG TRHKTLIRIM 310 320 330 340 VSRSEIDMNE IKVFYQKKYG IPLCQAILDE TKGDYEKILV ALCGGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat lipocortin I cDNA." Tamaki M., Nakamura E., Nishikubo C., Sakata T., Shin M., Teraoka H. Nucleic Acids Res. 15:7637-7637(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"Molecular cloning anD expression in Escherichia coli of the cDNA coding for rat lipocortin I (calpactin II)." Shimizu Y., Takabayashi E., Yano S., Shimizu N., Yamada K., Gushima H. Gene 65:141-147(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Correlation of gene and protein structure of rat and human lipocortin I." Kovacic R.T., Tizard R., Cate R.L., Frey A.Z., Wallner B.P. Biochemistry 30:9015-9021(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[5]	Bienvenut W.V., von Kriegsheim A., Kolch W. Submitted (JUN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-9; 59-71; 99-124; 129-144; 167-177; 186-204; 214-228; 251-281 AND 304-312, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Fibroblast.
[6]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 99-113; 129-144 AND 215-228, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00446 mRNA. Translation: CAA68500.1. M19967 mRNA. Translation: AAA40861.1. S57478 , S57447, S57450, S57455, S57459, S57463, S57466, S57468, S57470, S57472, S57474, S57476 Genomic DNA. Translation: AAB19866.1. BC061710 mRNA. Translation: AAH61710.1.
IPI	IPI00231615.
PIR	LURT1. JT0303.
RefSeq	NP_037036.1. NM_012904.2.
UniGene	Rn.1792.
3D structure databases
ProteinModelPortal	P07150.
SMR	P07150. Positions 2-344.
ModBase	Search...
Protein-protein interaction databases
IntAct	P07150. 2 interactions.
STRING	10116.ENSRNOP00000051674.
PTM databases
PhosphoSite	P07150.
Proteomic databases
PaxDb	P07150.
PRIDE	P07150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023664; ENSRNOP00000023664; ENSRNOG00000017469.
GeneID	25380.
KEGG	rno:25380.
UCSC	RGD:2118. rat.
Organism-specific databases
CTD	301.
RGD	2118. Anxa1.
Phylogenomic databases
eggNOG	NOG282829.
GeneTree	ENSGT00680000099735.
HOGENOM	HOG000158803.
HOVERGEN	HBG061815.
OrthoDB	EOG48WC2C.
Gene expression databases
ArrayExpress	P07150.
Genevestigator	P07150.
GermOnline	ENSRNOG00000017469. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.220.10. 4 hits.
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002388. AnnexinI. [Graphical view]
PANTHER	PTHR10502. PTHR10502. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00197. ANNEXINI.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	606425.

Entry information

Entry name

ANXA1_RAT

Accession

Primary (citable) accession number: P07150
Secondary accession number(s): Q64664

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program