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P07147

- TYRP1_MOUSE

UniProt

P07147 - TYRP1_MOUSE

Protein

5,6-dihydroxyindole-2-carboxylic acid oxidase

Gene

Tyrp1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.1 Publication

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi192 – 1921Copper ABy similarity
    Metal bindingi215 – 2151Copper ABy similarity
    Metal bindingi224 – 2241Copper ABy similarity
    Metal bindingi377 – 3771Copper BBy similarity
    Metal bindingi381 – 3811Copper BBy similarity
    Metal bindingi404 – 4041Copper BBy similarity

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen Source: InterPro
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. acetoacetic acid metabolic process Source: MGI
    2. melanin biosynthetic process from tyrosine Source: UniProtKB
    3. melanin metabolic process Source: MGI
    4. melanocyte differentiation Source: MGI
    5. melanosome organization Source: MGI
    6. pigmentation Source: MGI

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15620.
    UniPathwayiUPA00785.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,6-dihydroxyindole-2-carboxylic acid oxidase (EC:1.14.18.-)
    Short name:
    DHICA oxidase
    Alternative name(s):
    Brown locus protein
    Catalase B
    Tyrosinase-related protein 1
    Short name:
    TRP
    Short name:
    TRP-1
    Short name:
    TRP1
    Gene namesi
    Name:Tyrp1
    Synonyms:Tyrp-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:98881. Tyrp1.

    Subcellular locationi

    Melanosome membrane 1 Publication; Single-pass type I membrane protein 1 Publication
    Note: Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex.

    GO - Cellular componenti

    1. clathrin-coated endocytic vesicle membrane Source: Ensembl
    2. endosome membrane Source: Ensembl
    3. integral component of membrane Source: UniProtKB-KW
    4. melanosome Source: UniProtKB
    5. melanosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Defects in Tyrp1 are the cause of the brown (b) phenotype. Brown mice have a brown or hypopigmented coat.

    Keywords - Diseasei

    Albinism, Disease mutation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Chaini25 – 5375135,6-dihydroxyindole-2-carboxylic acid oxidasePRO_0000035890Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP07147.
    PRIDEiP07147.

    PTM databases

    PhosphoSiteiP07147.

    Expressioni

    Tissue specificityi

    Pigment cells.

    Gene expression databases

    BgeeiP07147.
    CleanExiMM_TYRP1.
    GenevestigatoriP07147.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TyrP113442EBI-821614,EBI-821603

    Protein-protein interaction databases

    IntActiP07147. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP07147.
    SMRiP07147. Positions 128-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini25 – 477453Lumenal, melanosomeSequence AnalysisAdd
    BLAST
    Topological domaini502 – 53736CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei478 – 50124HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG86242.
    HOGENOMiHOG000118376.
    HOVERGENiHBG003553.
    InParanoidiP07147.
    KOiK00506.
    OMAiVKKTFLG.
    PhylomeDBiP07147.
    TreeFamiTF315865.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR015559. DiOHindole_carboxylic_A_Oxase.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PANTHERiPTHR11474:SF3. PTHR11474:SF3. 1 hit.
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 2 hits.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P07147-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSYNVLPLA YISLFLMLFY QVWAQFPREC ANIEALRRGV CCPDLLPSSG    50
    PGTDPCGSSS GRGRCVAVIA DSRPHSRHYP HDGKDDREAW PLRFFNRTCQ 100
    CNDNFSGHNC GTCRPGWRGA ACNQKILTVR RNLLDLSPEE KSHFVRALDM 150
    AKRTTHPQFV IATRRLEDIL GPDGNTPQFE NISVYNYFVW THYYSVKKTF 200
    LGTGQESFGD VDFSHEGPAF LTWHRYHLLQ LERDMQEMLQ EPSFSLPYWN 250
    FATGKNVCDV CTDDLMGSRS NFDSTLISPN SVFSQWRVVC ESLEEYDTLG 300
    TLCNSTEGGP IRRNPAGNVG RPAVQRLPEP QDVTQCLEVR VFDTPPFYSN 350
    STDSFRNTVE GYSAPTGKYD PAVRSLHNLA HLFLNGTGGQ THLSPNDPIF 400
    VLLHTFTDAV FDEWLRRYNA DISTFPLENA PIGHNRQYNM VPFWPPVTNT 450
    EMFVTAPDNL GYAYEVQWPG QEFTVSEIIT IAVVAALLLV AAIFGVASCL 500
    IRSRSTKNEA NQPLLTDHYQ RYAEDYEELP NPNHSMV 537
    Length:537
    Mass (Da):60,761
    Last modified:April 1, 1988 - v1
    Checksum:i86570998AA9EB0BC
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti110 – 1101C → Y in brown. 1 Publication
    Natural varianti326 – 3261R → H in brown. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03687 mRNA. Translation: CAA27323.1.
    BC076598 mRNA. Translation: AAH76598.1.
    CCDSiCCDS18290.1.
    PIRiA24933. YRMSB6.
    RefSeqiNP_001268943.1. NM_001282014.1.
    NP_001268944.1. NM_001282015.1.
    NP_112479.1. NM_031202.3.
    UniGeneiMm.30438.

    Genome annotation databases

    EnsembliENSMUST00000006151; ENSMUSP00000006151; ENSMUSG00000005994.
    ENSMUST00000102831; ENSMUSP00000099895; ENSMUSG00000005994.
    GeneIDi22178.
    KEGGimmu:22178.
    UCSCiuc008tjq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03687 mRNA. Translation: CAA27323.1 .
    BC076598 mRNA. Translation: AAH76598.1 .
    CCDSi CCDS18290.1.
    PIRi A24933. YRMSB6.
    RefSeqi NP_001268943.1. NM_001282014.1.
    NP_001268944.1. NM_001282015.1.
    NP_112479.1. NM_031202.3.
    UniGenei Mm.30438.

    3D structure databases

    ProteinModelPortali P07147.
    SMRi P07147. Positions 128-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P07147. 1 interaction.

    PTM databases

    PhosphoSitei P07147.

    Proteomic databases

    MaxQBi P07147.
    PRIDEi P07147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006151 ; ENSMUSP00000006151 ; ENSMUSG00000005994 .
    ENSMUST00000102831 ; ENSMUSP00000099895 ; ENSMUSG00000005994 .
    GeneIDi 22178.
    KEGGi mmu:22178.
    UCSCi uc008tjq.2. mouse.

    Organism-specific databases

    CTDi 7306.
    MGIi MGI:98881. Tyrp1.

    Phylogenomic databases

    eggNOGi NOG86242.
    HOGENOMi HOG000118376.
    HOVERGENi HBG003553.
    InParanoidi P07147.
    KOi K00506.
    OMAi VKKTFLG.
    PhylomeDBi P07147.
    TreeFami TF315865.

    Enzyme and pathway databases

    UniPathwayi UPA00785 .
    BioCyci MetaCyc:MONOMER-15620.

    Miscellaneous databases

    ChiTaRSi TYRP1. mouse.
    NextBioi 302135.
    PROi P07147.
    SOURCEi Search...

    Gene expression databases

    Bgeei P07147.
    CleanExi MM_TYRP1.
    Genevestigatori P07147.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR015559. DiOHindole_carboxylic_A_Oxase.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    PANTHERi PTHR11474:SF3. PTHR11474:SF3. 1 hit.
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 2 hits.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Melanoma.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    3. "A cDNA encoding tyrosinase-related protein maps to the brown locus in mouse."
      Jackson I.J.
      Proc. Natl. Acad. Sci. U.S.A. 85:4392-4396(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROTEIN.
    4. "Murine and human b locus pigmentation genes encode a glycoprotein (gp75) with catalase activity."
      Halaban R., Moellmann G.
      Proc. Natl. Acad. Sci. U.S.A. 87:4809-4813(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    5. "The mouse brown (b) locus protein has dopachrome tautomerase activity and is located in lysosomes in transfected fibroblasts."
      Winder A.J., Wittbjer A., Rosengren E., Rorsman H.
      J. Cell Sci. 106:153-166(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    6. "Tyrosinase related protein 1 (TRP1) functions as a DHICA oxidase in melanin biosynthesis."
      Kobayashi T., Urabe K., Winder A., Jimenez-Cervantes C., Imokawa G., Brewington T., Solano F., Garcia-Borron J.C., Hearing V.J.
      EMBO J. 13:5818-5825(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles."
      Setty S.R., Tenza D., Truschel S.T., Chou E., Sviderskaya E.V., Theos A.C., Lamoreux M.L., Di Pietro S.M., Starcevic M., Bennett D.C., Dell'Angelica E.C., Raposo G., Marks M.S.
      Mol. Biol. Cell 18:768-780(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "The molecular basis of brown, an old mouse mutation, and of an induced revertant to wild type."
      Zdarsky E., Favor J., Jackson I.J.
      Genetics 126:443-449(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS BROWN TYR-110 AND HIS-326.

    Entry informationi

    Entry nameiTYRP1_MOUSE
    AccessioniPrimary (citable) accession number: P07147
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3