Reviewed, UniProtKB/Swiss-Prot P07146 (TRY2_MOUSE)

Names and origin

Protein names

Recommended name:
    Anionic trypsin-2
    EC=3.4.21.4
Alternative name(s):
    Anionic trypsin II
    Pretrypsinogen II
    Serine protease 2

Gene names

Name:	Prss2
Synonyms:	Try2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from sequence or structural similarity. Source: UniProtKB digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from sequence or structural similarity. Source: UniProtKB serine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028203
	Chain	24 – 246	223	Anionic trypsin-2		PRO_0000028204
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	200	1	Charge relay system By similarity
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	132 ↔ 233		By similarity
	Disulfide bond	139 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07146-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: CEF8C97AAC2D07AD	FASTA	246	26,204
	10 20 30 40 50 60 MSALLILALV GAAVAFPVDD DDKIVGGYTC RESSVPYQVS LNAGYHFCGG SLINDQWVVS 70 80 90 100 110 120 AAHCYKYRIQ VRLGEHNINV LEGNEQFVDS AKIIRHPNYN SWTLDNDIML IKLASPVTLN 130 140 150 160 170 180 ARVASVPLPS SCAPAGTQCL ISGWGNTLSN GVNNPDLLQC VDAPVLPQAD CEASYPGDIT 190 200 210 220 230 240 NNMICVGFLE GGKDSCQGDS GGPVVCNGEL QGIVSWGYGC AQPDAPGVYT KVCNYVDWIQ NTIADN

References

[1]	"Sequence organisation and transcriptional regulation of the mouse elastase II and trypsin genes." Stevenson B.J., Hagenbuechle O., Wellauer P.K. Nucleic Acids Res. 14:8307-8330(1986) [PubMed: 3641189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: A/J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04574 mRNA. Translation: CAA28243.1. X04577 Genomic DNA. Translation: CAA28245.1.
IPI	IPI00403650.
PIR	B25528.
RefSeq	NP_033456.1.
UniGene	Mm.276926
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	P07146. Positions 24-246.
ModBase	Search...
Protein family/group databases
MEROPS	S01.064.
PTM databases
PhosphoSite	P07146.
Genome annotation databases
Ensembl	ENSMUSG00000057163. Mus musculus. [Contig view]
GeneID	22072.
KEGG	mmu:22072.
Organism-specific databases
MGI	MGI:102759. Prss2.
Phylogenomic databases
HOGENOM	P07146.
HOVERGEN	P07146.
OMA	P07146. LLQCVDA.
Enzyme and pathway databases
BRENDA	3.4.21.4. 244.
Gene expression databases
ArrayExpress	P07146.
Bgee	P07146.
CleanEx	MM_PRSS2.
GermOnline	ENSMUSG00000057163. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301902.
SOURCE	Search...

Entry information

Entry name

TRY2_MOUSE

Accession

Primary (citable) accession number: P07146

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 5, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)