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Protein

Homoserine kinase

Gene

thrB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.By similarity

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.

Pathwayi: L-threonine biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes L-threonine from L-aspartate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. Homoserine dehydrogenase (hom)
  4. Homoserine kinase (thrB)
  5. Threonine synthase (thrC)
This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 105ATPSequence analysisAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Threonine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-10757-MONOMER
UniPathwayiUPA00050; UER00064

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Short name:
HK
Short name:
HSK
Gene namesi
Name:thrB
Ordered Locus Names:Cgl1184, cg1338
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001565652 – 309Homoserine kinaseAdd BLAST308

2D gel databases

World-2DPAGE0001:P07128

Interactioni

Protein-protein interaction databases

STRINGi196627.cg1338

Structurei

Secondary structure

1309
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 20Combined sources11
Turni22 – 24Combined sources3
Turni26 – 28Combined sources3
Beta strandi29 – 43Combined sources15
Beta strandi45 – 53Combined sources9
Helixi65 – 78Combined sources14
Beta strandi84 – 92Combined sources9
Beta strandi98 – 100Combined sources3
Helixi102 – 117Combined sources16
Helixi124 – 132Combined sources9
Beta strandi134 – 136Combined sources3
Helixi138 – 146Combined sources9
Beta strandi148 – 155Combined sources8
Beta strandi159 – 161Combined sources3
Beta strandi165 – 170Combined sources6
Beta strandi178 – 183Combined sources6
Beta strandi193 – 197Combined sources5
Helixi203 – 221Combined sources19
Helixi224 – 226Combined sources3
Helixi227 – 230Combined sources4
Beta strandi234 – 236Combined sources3
Helixi237 – 243Combined sources7
Helixi245 – 256Combined sources12
Beta strandi260 – 263Combined sources4
Beta strandi270 – 276Combined sources7
Helixi280 – 288Combined sources9
Beta strandi292 – 298Combined sources7
Beta strandi303 – 306Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WATX-ray2.14A/B1-309[»]
ProteinModelPortaliP07128
SMRiP07128
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D5I Bacteria
COG0083 LUCA
HOGENOMiHOG000247199
KOiK00872
OMAiPDNVAPC

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00384 Homoser_kinase, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR006203 GHMP_knse_ATP-bd_CS
IPR000870 Homoserine_kinase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PANTHERiPTHR20861:SF1 PTHR20861:SF1, 1 hit
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF000676 Homoser_kin, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00191 thrB, 1 hit
PROSITEiView protein in PROSITE
PS00627 GHMP_KINASES_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIELNVGRK VTVTVPGSSA NLGPGFDTLG LALSVYDTVE VEIIPSGLEV
60 70 80 90 100
EVFGEGQGEV PLDGSHLVVK AIRAGLKAAD AEVPGLRVVC HNNIPQSRGL
110 120 130 140 150
GSSAAAAVAG VAAANGLADF PLTQEQIVQL SSAFEGHPDN AAASVLGGAV
160 170 180 190 200
VSWTNLSIDG KSQPQYAAVP LEVQDNIRAT ALVPNFHAST EAVRRVLPTE
210 220 230 240 250
VTHIDARFNV SRVAVMIVAL QQRPDLLWEG TRDRLHQPYR AEVLPITSEW
260 270 280 290 300
VNRLRNRGYA AYLSGAGPTA MVLSTEPIPD KVLEDARESG IKVLELEVAG

PVKVEVNQP
Length:309
Mass (Da):32,620
Last modified:January 23, 2007 - v4
Checksum:i89C807BC983A60E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246I → V in CAA68332 (PubMed:3035505).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00546 Genomic DNA Translation: CAA68615.1
Y00140 Genomic DNA Translation: CAA68332.1
BA000036 Genomic DNA Translation: BAB98577.1
BX927151 Genomic DNA Translation: CAF19888.1
PIRiS00866 KIFKMG
RefSeqiNP_600410.1, NC_003450.3
WP_011014183.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB98577; BAB98577; BAB98577
CAF19888; CAF19888; cg1338
GeneIDi1019167
KEGGicgb:cg1338
cgl:NCgl1137
PATRICifig|196627.13.peg.1163

Similar proteinsi

Entry informationi

Entry nameiKHSE_CORGL
AccessioniPrimary (citable) accession number: P07128
Secondary accession number(s): P08210
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 142 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health