Reviewed, UniProtKB/Swiss-Prot P07128 (KHSE_CORGL)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	Cgl1184, cg1338

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 309	308	Homoserine kinase HAMAP MF_00384		PRO_0000156565
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Experimental info
	Sequence conflict	246	1	I → V in CAA68332. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P07128-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 89C807BC983A60E7	FASTA	309	32,620
	10 20 30 40 50 60 MAIELNVGRK VTVTVPGSSA NLGPGFDTLG LALSVYDTVE VEIIPSGLEV EVFGEGQGEV 70 80 90 100 110 120 PLDGSHLVVK AIRAGLKAAD AEVPGLRVVC HNNIPQSRGL GSSAAAAVAG VAAANGLADF 130 140 150 160 170 180 PLTQEQIVQL SSAFEGHPDN AAASVLGGAV VSWTNLSIDG KSQPQYAAVP LEVQDNIRAT 190 200 210 220 230 240 ALVPNFHAST EAVRRVLPTE VTHIDARFNV SRVAVMIVAL QQRPDLLWEG TRDRLHQPYR 250 260 270 280 290 300 AEVLPITSEW VNRLRNRGYA AYLSGAGPTA MVLSTEPIPD KVLEDARESG IKVLELEVAG PVKVEVNQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon." Peoples O.P., Liebl W., Bodis M., Maeng P.J., Follettie M.T., Archer J.A.C., Sinskey A.J. Mol. Microbiol. 2:63-72(1988) [PubMed: 2835591] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"Nucleotide sequence of the homoserine kinase (thr B) gene of Brevibacterium lactofermentum." Mateos L.M., del Real G., Aguilar A., Martin J.F. Nucleic Acids Res. 15:3922-3922(1987) [PubMed: 3035505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[3]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00546 Genomic DNA. Translation: CAA68615.1. Y00140 Genomic DNA. Translation: CAA68332.1. BA000036 Genomic DNA. Translation: BAB98577.1. BX927151 Genomic DNA. Translation: CAF19888.1.
PIR	KIFKMG. S00866.
RefSeq	NP_600410.1. YP_225474.1.
3D structure databases
ModBase	Search...
2-D gel databases
World-2DPAGE	0001:P07128.
Proteomic databases
PRIDE	P07128.
Genome annotation databases
GeneID	1019167. 3345034.
GenomeReviews	Gene locus Cgl1184 in contig BA000036_GR. Gene locus cg1338 in contig BX927147_GR.
KEGG	cgb:cg1338. cgl:NCgl1137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P07128.
OMA	P07128. FDCLGAA.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG1338-MON.
BRENDA	2.7.1.39. 812.
Family and domain databases
HAMAP	MF_00384. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR014721. Ribosomal_S5_D2-type_fold. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CORGL

Accession

Primary (citable) accession number: P07128
Secondary accession number(s): P08210

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 83 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)