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Protein

Sodium/proline symporter

Gene

putP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei344Involved in high-affinity binding for sodium and proline1

GO - Molecular functioni

  • proline:sodium symporter activity Source: EcoCyc
  • sodium ion binding Source: InterPro

GO - Biological processi

  • proline transport Source: EcoCyc
  • short-chain fatty acid transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:PUTP-MONOMER.
ECOL316407:JW1001-MONOMER.
MetaCyc:PUTP-MONOMER.

Protein family/group databases

TCDBi2.A.21.2.1. the solute:sodium symporter (sss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/proline symporter
Alternative name(s):
Proline permease
Propionate transporter
Gene namesi
Name:putP
Ordered Locus Names:b1015, JW1001
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10802. putP.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 26HelicalSequence analysisAdd BLAST23
Topological domaini27 – 66PeriplasmicSequence analysisAdd BLAST40
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 124CytoplasmicSequence analysisAdd BLAST37
Transmembranei125 – 150HelicalSequence analysisAdd BLAST26
Topological domaini151 – 162PeriplasmicSequence analysisAdd BLAST12
Transmembranei163 – 184HelicalSequence analysisAdd BLAST22
Topological domaini185 – 189CytoplasmicSequence analysis5
Transmembranei190 – 213HelicalSequence analysisAdd BLAST24
Topological domaini214 – 231PeriplasmicSequence analysisAdd BLAST18
Transmembranei232 – 248HelicalSequence analysisAdd BLAST17
Topological domaini249 – 274CytoplasmicSequence analysisAdd BLAST26
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Topological domaini296 – 319PeriplasmicSequence analysisAdd BLAST24
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 370CytoplasmicSequence analysisAdd BLAST30
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Topological domaini392 – 397PeriplasmicSequence analysis6
Transmembranei398 – 423HelicalSequence analysisAdd BLAST26
Topological domaini424 – 430CytoplasmicSequence analysis7
Transmembranei431 – 445HelicalSequence analysisAdd BLAST15
Topological domaini446 – 448PeriplasmicSequence analysis3
Transmembranei449 – 475HelicalSequence analysisAdd BLAST27
Topological domaini476 – 502CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate when grown on propionate as sole carbon source.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257R → C: Sodium-independent binding affinity for proline. 1
Mutagenesisi376R → E or Q: No change in activity. 1 Publication1
Mutagenesisi376R → K: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001053981 – 502Sodium/proline symporterAdd BLAST502

Proteomic databases

EPDiP07117.
PaxDbiP07117.
PRIDEiP07117.

Expressioni

Inductioni

Up-regulated by propionate.1 Publication

Interactioni

Subunit structurei

Has been isolated from inner membrane preparations as a homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260051. 9 interactors.
IntActiP07117. 4 interactors.
STRINGi511145.b1015.

Structurei

3D structure databases

ProteinModelPortaliP07117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 66HydrophilicAdd BLAST40
Regioni88 – 124HydrophilicAdd BLAST37
Regioni151 – 162HydrophilicAdd BLAST12
Regioni185 – 189Hydrophilic5
Regioni214 – 231HydrophilicAdd BLAST18
Regioni249 – 274HydrophilicAdd BLAST26
Regioni296 – 319HydrophilicAdd BLAST24
Regioni341 – 370HydrophilicAdd BLAST30
Regioni392 – 397Hydrophilic6
Regioni424 – 430Hydrophilic7
Regioni446 – 448Hydrophilic3
Regioni476 – 502HydrophilicAdd BLAST27

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK7. Bacteria.
COG0591. LUCA.
HOGENOMiHOG000282935.
InParanoidiP07117.
KOiK11928.
OMAiEYSTALW.
PhylomeDBiP07117.

Family and domain databases

CDDicd11475. SLC5sbd_PutP. 1 hit.
InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PTHR11819:SF148. PTHR11819:SF148. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISTPMLVT FCVYIFGMIL IGFIAWRSTK NFDDYILGGR SLGPFVTALS
60 70 80 90 100
AGASDMSGWL LMGLPGAVFL SGISESWIAI GLTLGAWINW KLVAGRLRVH
110 120 130 140 150
TEYNNNALTL PDYFTGRFED KSRILRIISA LVILLFFTIY CASGIVAGAR
160 170 180 190 200
LFESTFGMSY ETALWAGAAA TILYTFIGGF LAVSWTDTVQ ASLMIFALIL
210 220 230 240 250
TPVIVIISVG GFGDSLEVIK QKSIENVDML KGLNFVAIIS LMGWGLGYFG
260 270 280 290 300
QPHILARFMA ADSHHSIVHA RRISMTWMIL CLAGAVAVGF FGIAYFNDHP
310 320 330 340 350
ALAGAVNQNA ERVFIELAQI LFNPWIAGIL LSAILAAVMS TLSCQLLVCS
360 370 380 390 400
SAITEDLYKA FLRKHASQKE LVWVGRVMVL VVALVAIALA ANPENRVLGL
410 420 430 440 450
VSYAWAGFGA AFGPVVLFSV MWSRMTRNGA LAGMIIGALT VIVWKQFGWL
460 470 480 490 500
GLYEIIPGFI FGSIGIVVFS LLGKAPSAAM QKRFAEADAH YHSAPPSRLQ

ES
Length:502
Mass (Da):54,344
Last modified:April 1, 1988 - v1
Checksum:i37AE923C33186356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05653 Genomic DNA. Translation: CAA29143.1.
U00096 Genomic DNA. Translation: AAC74100.1.
AP009048 Genomic DNA. Translation: BAA35793.1.
L01150 Genomic DNA. Translation: AAA24463.1.
L01151 Genomic DNA. Translation: AAA24464.1.
M35174 Genomic DNA. Translation: AAA24458.1.
PIRiA30258. JGECPP.
RefSeqiNP_415535.1. NC_000913.3.
WP_001018479.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74100; AAC74100; b1015.
BAA35793; BAA35793; BAA35793.
GeneIDi945602.
KEGGiecj:JW1001.
eco:b1015.
PATRICi32117263. VBIEscCol129921_1055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05653 Genomic DNA. Translation: CAA29143.1.
U00096 Genomic DNA. Translation: AAC74100.1.
AP009048 Genomic DNA. Translation: BAA35793.1.
L01150 Genomic DNA. Translation: AAA24463.1.
L01151 Genomic DNA. Translation: AAA24464.1.
M35174 Genomic DNA. Translation: AAA24458.1.
PIRiA30258. JGECPP.
RefSeqiNP_415535.1. NC_000913.3.
WP_001018479.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260051. 9 interactors.
IntActiP07117. 4 interactors.
STRINGi511145.b1015.

Protein family/group databases

TCDBi2.A.21.2.1. the solute:sodium symporter (sss) family.

Proteomic databases

EPDiP07117.
PaxDbiP07117.
PRIDEiP07117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74100; AAC74100; b1015.
BAA35793; BAA35793; BAA35793.
GeneIDi945602.
KEGGiecj:JW1001.
eco:b1015.
PATRICi32117263. VBIEscCol129921_1055.

Organism-specific databases

EchoBASEiEB0795.
EcoGeneiEG10802. putP.

Phylogenomic databases

eggNOGiENOG4105CK7. Bacteria.
COG0591. LUCA.
HOGENOMiHOG000282935.
InParanoidiP07117.
KOiK11928.
OMAiEYSTALW.
PhylomeDBiP07117.

Enzyme and pathway databases

BioCyciEcoCyc:PUTP-MONOMER.
ECOL316407:JW1001-MONOMER.
MetaCyc:PUTP-MONOMER.

Miscellaneous databases

PROiP07117.

Family and domain databases

CDDicd11475. SLC5sbd_PutP. 1 hit.
InterProiIPR011851. Na/Pro_symporter.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PTHR11819:SF148. PTHR11819:SF148. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02121. Na_Pro_sym. 1 hit.
TIGR00813. sss. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUTP_ECOLI
AccessioniPrimary (citable) accession number: P07117
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.