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Protein

Sodium/proline symporter

Gene

putP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei344Involved in high-affinity binding for sodium and proline1

GO - Molecular functioni

  • proline:sodium symporter activity Source: EcoCyc
  • sodium ion binding Source: InterPro

GO - Biological processi

  • proline transport Source: EcoCyc
  • short-chain fatty acid transport Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Ion transport, Sodium transport, Symport, Transport
LigandSodium

Enzyme and pathway databases

BioCyciEcoCyc:PUTP-MONOMER
MetaCyc:PUTP-MONOMER

Protein family/group databases

TCDBi2.A.21.2.1 the solute:sodium symporter (sss) family

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/proline symporter
Alternative name(s):
Proline permease
Propionate transporter
Gene namesi
Name:putP
Ordered Locus Names:b1015, JW1001
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10802 putP

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 26HelicalSequence analysisAdd BLAST23
Topological domaini27 – 66PeriplasmicSequence analysisAdd BLAST40
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 124CytoplasmicSequence analysisAdd BLAST37
Transmembranei125 – 150HelicalSequence analysisAdd BLAST26
Topological domaini151 – 162PeriplasmicSequence analysisAdd BLAST12
Transmembranei163 – 184HelicalSequence analysisAdd BLAST22
Topological domaini185 – 189CytoplasmicSequence analysis5
Transmembranei190 – 213HelicalSequence analysisAdd BLAST24
Topological domaini214 – 231PeriplasmicSequence analysisAdd BLAST18
Transmembranei232 – 248HelicalSequence analysisAdd BLAST17
Topological domaini249 – 274CytoplasmicSequence analysisAdd BLAST26
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Topological domaini296 – 319PeriplasmicSequence analysisAdd BLAST24
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 370CytoplasmicSequence analysisAdd BLAST30
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Topological domaini392 – 397PeriplasmicSequence analysis6
Transmembranei398 – 423HelicalSequence analysisAdd BLAST26
Topological domaini424 – 430CytoplasmicSequence analysis7
Transmembranei431 – 445HelicalSequence analysisAdd BLAST15
Topological domaini446 – 448PeriplasmicSequence analysis3
Transmembranei449 – 475HelicalSequence analysisAdd BLAST27
Topological domaini476 – 502CytoplasmicSequence analysisAdd BLAST27

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate when grown on propionate as sole carbon source.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257R → C: Sodium-independent binding affinity for proline. 1
Mutagenesisi376R → E or Q: No change in activity. 1 Publication1
Mutagenesisi376R → K: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001053981 – 502Sodium/proline symporterAdd BLAST502

Proteomic databases

EPDiP07117
PaxDbiP07117
PRIDEiP07117

Expressioni

Inductioni

Up-regulated by propionate.1 Publication

Interactioni

Subunit structurei

Has been isolated from inner membrane preparations as a homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260051, 14 interactors
IntActiP07117, 4 interactors
STRINGi316385.ECDH10B_1087

Structurei

3D structure databases

ProteinModelPortaliP07117
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 66HydrophilicAdd BLAST40
Regioni88 – 124HydrophilicAdd BLAST37
Regioni151 – 162HydrophilicAdd BLAST12
Regioni185 – 189Hydrophilic5
Regioni214 – 231HydrophilicAdd BLAST18
Regioni249 – 274HydrophilicAdd BLAST26
Regioni296 – 319HydrophilicAdd BLAST24
Regioni341 – 370HydrophilicAdd BLAST30
Regioni392 – 397Hydrophilic6
Regioni424 – 430Hydrophilic7
Regioni446 – 448Hydrophilic3
Regioni476 – 502HydrophilicAdd BLAST27

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK7 Bacteria
COG0591 LUCA
HOGENOMiHOG000282935
InParanoidiP07117
KOiK11928
OMAiCWTDLIQ
PhylomeDBiP07117

Family and domain databases

CDDicd11475 SLC5sbd_PutP, 1 hit
Gene3Di1.20.1730.10, 1 hit
InterProiView protein in InterPro
IPR038377 Na/Glc_symporter_sf
IPR011851 Na/Pro_symporter
IPR001734 Na/solute_symporter
IPR018212 Na/solute_symporter_CS
PfamiView protein in Pfam
PF00474 SSF, 1 hit
TIGRFAMsiTIGR02121 Na_Pro_sym, 1 hit
TIGR00813 sss, 1 hit
PROSITEiView protein in PROSITE
PS00456 NA_SOLUT_SYMP_1, 1 hit
PS00457 NA_SOLUT_SYMP_2, 1 hit
PS50283 NA_SOLUT_SYMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

P07117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISTPMLVT FCVYIFGMIL IGFIAWRSTK NFDDYILGGR SLGPFVTALS
60 70 80 90 100
AGASDMSGWL LMGLPGAVFL SGISESWIAI GLTLGAWINW KLVAGRLRVH
110 120 130 140 150
TEYNNNALTL PDYFTGRFED KSRILRIISA LVILLFFTIY CASGIVAGAR
160 170 180 190 200
LFESTFGMSY ETALWAGAAA TILYTFIGGF LAVSWTDTVQ ASLMIFALIL
210 220 230 240 250
TPVIVIISVG GFGDSLEVIK QKSIENVDML KGLNFVAIIS LMGWGLGYFG
260 270 280 290 300
QPHILARFMA ADSHHSIVHA RRISMTWMIL CLAGAVAVGF FGIAYFNDHP
310 320 330 340 350
ALAGAVNQNA ERVFIELAQI LFNPWIAGIL LSAILAAVMS TLSCQLLVCS
360 370 380 390 400
SAITEDLYKA FLRKHASQKE LVWVGRVMVL VVALVAIALA ANPENRVLGL
410 420 430 440 450
VSYAWAGFGA AFGPVVLFSV MWSRMTRNGA LAGMIIGALT VIVWKQFGWL
460 470 480 490 500
GLYEIIPGFI FGSIGIVVFS LLGKAPSAAM QKRFAEADAH YHSAPPSRLQ

ES
Length:502
Mass (Da):54,344
Last modified:April 1, 1988 - v1
Checksum:i37AE923C33186356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05653 Genomic DNA Translation: CAA29143.1
U00096 Genomic DNA Translation: AAC74100.1
AP009048 Genomic DNA Translation: BAA35793.1
L01150 Genomic DNA Translation: AAA24463.1
L01151 Genomic DNA Translation: AAA24464.1
M35174 Genomic DNA Translation: AAA24458.1
PIRiA30258 JGECPP
RefSeqiNP_415535.1, NC_000913.3
WP_001018479.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74100; AAC74100; b1015
BAA35793; BAA35793; BAA35793
GeneIDi945602
KEGGiecj:JW1001
eco:b1015
PATRICifig|1411691.4.peg.1254

Similar proteinsi

Entry informationi

Entry nameiPUTP_ECOLI
AccessioniPrimary (citable) accession number: P07117
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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