##gff-version 3 P07112 UniProtKB Initiator methionine 1 1 . . . Note=Removed;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8223650;Dbxref=PMID:8223650 P07112 UniProtKB Chain 2 709 . . . ID=PRO_0000210849;Note=Mobilization protein A P07112 UniProtKB Region 1 21 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P07112 UniProtKB Region 2 186 . . . Note=DNA relaxase P07112 UniProtKB Region 251 337 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P07112 UniProtKB Region 315 709 . . . Note=DNA primase P07112 UniProtKB Region 689 709 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P07112 UniProtKB Coiled coil 567 594 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255 P07112 UniProtKB Compositional bias 251 285 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P07112 UniProtKB Compositional bias 323 337 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P07112 UniProtKB Active site 25 25 . . . Note=O-(5'-phospho-DNA)-tyrosine intermediate%3B for relaxase activity P07112 UniProtKB Binding site 112 112 . . . . P07112 UniProtKB Binding site 120 120 . . . . P07112 UniProtKB Binding site 122 122 . . . . P07112 UniProtKB Site 70 70 . . . Note=Involved in DNA binding;Ontology_term=ECO:0000305;evidence=ECO:0000305 P07112 UniProtKB Mutagenesis 25 25 . . . Note=99.5%25 decrease in conjugal transfer frequency. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Mutagenesis 32 32 . . . Note=No decrease in conjugal transfer frequency. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Mutagenesis 38 38 . . . Note=No decrease in conjugal transfer frequency. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Mutagenesis 74 74 . . . Note=50%25 decrease in conjugal transfer frequency. 10-fold decrease in conjugal transfer frequency and great reduction in the rate of DNA cleavage%3B when associated with A-76. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Mutagenesis 74 74 . . . Note=70%25 decrease in conjugal transfer frequency. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Mutagenesis 76 76 . . . Note=No decrease in conjugal transfer frequency. 10-fold decrease in conjugal transfer frequency and great reduction in the rate of DNA cleavage%3B when associated with A-74. E->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17157875;Dbxref=PMID:17157875 P07112 UniProtKB Sequence conflict 291 291 . . . Note=P->Q;Ontology_term=ECO:0000305;evidence=ECO:0000305