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Protein

Outer membrane usher protein PapC

Gene

papC

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the export and assembly of pili subunits across the outer membrane. Forms a hexameric ring-shaped pore in the outer bacterial membrane. The 2 nanometer-diameter pore allows the passage of the thin tip fibrillum. As for the rod, it probably unwinds into linear fibers which would therefore be narrow enough to pass through the pore.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Protein family/group databases

TCDBi1.B.11.2.1. the outer membrane fimbrial usher porin (fup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein PapC
Gene namesi
Name:papC
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 836812Outer membrane usher protein PapCPRO_0000009323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi814 ↔ 832Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP07110.

Interactioni

Protein-protein interaction databases

DIPiDIP-60749N.
STRINGi199310.c5186.

Structurei

Secondary structure

1
836
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi174 – 18512Combined sources
Beta strandi192 – 20312Combined sources
Beta strandi208 – 2158Combined sources
Beta strandi218 – 2214Combined sources
Beta strandi228 – 2314Combined sources
Beta strandi234 – 24411Combined sources
Turni245 – 2484Combined sources
Beta strandi249 – 2579Combined sources
Beta strandi261 – 2633Combined sources
Beta strandi267 – 2759Combined sources
Helixi282 – 2843Combined sources
Beta strandi293 – 2986Combined sources
Beta strandi300 – 3056Combined sources
Beta strandi308 – 3147Combined sources
Beta strandi317 – 3204Combined sources
Beta strandi331 – 3388Combined sources
Beta strandi347 – 3504Combined sources
Beta strandi363 – 3719Combined sources
Beta strandi383 – 39210Combined sources
Beta strandi395 – 40612Combined sources
Beta strandi409 – 41911Combined sources
Beta strandi421 – 4233Combined sources
Beta strandi425 – 43612Combined sources
Turni438 – 4403Combined sources
Beta strandi442 – 45211Combined sources
Beta strandi464 – 4718Combined sources
Helixi478 – 4858Combined sources
Beta strandi494 – 50512Combined sources
Turni507 – 5104Combined sources
Beta strandi512 – 52514Combined sources
Beta strandi528 – 53710Combined sources
Beta strandi548 – 5547Combined sources
Beta strandi565 – 5728Combined sources
Beta strandi579 – 5846Combined sources
Beta strandi590 – 59910Combined sources
Helixi601 – 6033Combined sources
Beta strandi606 – 61510Combined sources
Beta strandi622 – 63211Combined sources
Beta strandi634 – 64512Combined sources
Beta strandi648 – 66114Combined sources
Beta strandi758 – 7647Combined sources
Beta strandi775 – 7773Combined sources
Beta strandi783 – 7875Combined sources
Beta strandi792 – 7965Combined sources
Beta strandi806 – 8105Combined sources
Beta strandi814 – 8185Combined sources
Beta strandi824 – 83411Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KT6NMR-A752-836[»]
2VQIX-ray3.20A/B157-667[»]
3FIPX-ray3.15A/B171-650[»]
ProteinModelPortaliP07110.
SMRiP07110. Positions 173-663.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07110.

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4106PG1. Bacteria.
COG3188. LUCA.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRIPFAVN NITCVILLSL FCNAASAVEF NTDVLDAADK KNIDFTRFSE
60 70 80 90 100
AGYVLPGQYL LDVIVNGQSI SPASLQISFV EPALSGDKAE KKLPQACLTS
110 120 130 140 150
DMVRLMGLTA ESLDKVVYWH DGQCADFHGL PGVDIRPDTG AGVLRINMPQ
160 170 180 190 200
AWLEYSDATW LPPSRWDDGI PGLMLDYNLN GTVSRNYQGG DSHQFSYNGT
210 220 230 240 250
VGGNLGPWRL RADYQGSQEQ SRYNGEKTTN RNFTWSRFYL FRAIPRWRAN
260 270 280 290 300
LTLGENNINS DIFRSWSYTG ASLESDDRML PPRLRGYAPQ ITGIAETNAR
310 320 330 340 350
VVVSQQGRVL YDSMVPAGPF SIQDLDSSVR GRLDVEVIEQ NGRKKTFQVD
360 370 380 390 400
TASVPYLTRP GQVRYKLVSG RSRGYGHETE GPVFATGEAS WGLSNQWSLY
410 420 430 440 450
GGAVLAGDYN ALAAGAGWDL GVPGTLSADI TQSVARIEGE RTFQGKSWRL
460 470 480 490 500
SYSKRFDNAD ADITFAGYRF SERNYMTMEQ YLNARYRNDY SSREKEMYTV
510 520 530 540 550
TLNKNVADWN TSFNLQYSRQ TYWDIRKTDY YTVSVNRYFN VFGLQGVAVG
560 570 580 590 600
LSASRSKYLG RDNDSAYLRI SVPLGTGTAS YSGSMSNDRY VNMAGYTDTF
610 620 630 640 650
NDGLDSYSLN AGLNSGGGLT SQRQINAYYS HRSPLANLSA NIASLQKGYT
660 670 680 690 700
SFGVSASGGA TITGKGAALH AGGMSGGTRL LVDTDGVGGV PVDGGQVVTN
710 720 730 740 750
RWGTGVVTDI SSYYRNTTSV DLKRLPDDVE ATRSVVESAL TEGAIGYRKF
760 770 780 790 800
SVLKGKRLFA ILRLADGSQP PFGASVTSEK GRELGMVADE GLAWLSGVTP
810 820 830
GETLSVNWDG KIQCQVNVPE TAISDQQLLL PCTPQK
Length:836
Mass (Da):91,509
Last modified:November 28, 2002 - v2
Checksum:i9343D35DC7B1E4C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti517 – 54327YSRQT…FNVFG → SENIKVAVDAHRIIVRFPYV PVCLTAV (PubMed:2897064).CuratedAdd
BLAST
Sequence conflicti517 – 54327YSRQT…FNVFG → SENIKVAVDAHRIIVRFPYV PVCLTAV (PubMed:1357526).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00529 Genomic DNA. Translation: CAA68588.1.
X61239 Genomic DNA. Translation: CAA43564.1.
AF481883 Genomic DNA. Translation: AAL89688.1.
PIRiS25218.
RefSeqiWP_000654936.1. NZ_LRVT01000069.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00529 Genomic DNA. Translation: CAA68588.1.
X61239 Genomic DNA. Translation: CAA43564.1.
AF481883 Genomic DNA. Translation: AAL89688.1.
PIRiS25218.
RefSeqiWP_000654936.1. NZ_LRVT01000069.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KT6NMR-A752-836[»]
2VQIX-ray3.20A/B157-667[»]
3FIPX-ray3.15A/B171-650[»]
ProteinModelPortaliP07110.
SMRiP07110. Positions 173-663.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60749N.
STRINGi199310.c5186.

Protein family/group databases

TCDBi1.B.11.2.1. the outer membrane fimbrial usher porin (fup) family.

Proteomic databases

PRIDEiP07110.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106PG1. Bacteria.
COG3188. LUCA.

Miscellaneous databases

EvolutionaryTraceiP07110.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence, regulation and functional analysis of the papC gene required for cell surface localization of Pap pili of uropathogenic Escherichia coli."
    Norgren M., Baga M., Tennent J.M., Normark S.
    Mol. Microbiol. 1:169-178(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700336 / J96.
  2. "Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific adhesive properties."
    Marklund B.-I., Tennent J.M., Garcia E., Hamers A., Baga M., Lindberg F., Gaastra W., Normark S.
    Mol. Microbiol. 6:2225-2242(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700336 / J96.
  3. "Bacterial outer membrane ushers contain distinct targeting and assembly domains for pilus biogenesis."
    Thanassi D.G., Stathopoulos C., Dodson K., Geiger D., Hultgren S.J.
    J. Bacteriol. 184:6260-6269(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 517-543.
  4. "PapD, a periplasmic transport protein in P-pilus biogenesis."
    Lindberg F., Tennent J.M., Hultgren S.J., Lund B., Normark S.
    J. Bacteriol. 171:6052-6058(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 720-836.
  5. "Chaperone-assisted self-assembly of pili independent of cellular energy."
    Jacob-Dubuisson F., Striker R., Hultgren S.J.
    J. Biol. Chem. 269:12447-12455(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane."
    Thanassi D.G., Saulino E.T., Lombardo M.J., Roth R., Heuser J., Hultgren S.J.
    Proc. Natl. Acad. Sci. U.S.A. 95:3146-3151(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiPAPC_ECOLX
AccessioniPrimary (citable) accession number: P07110
Secondary accession number(s): Q8RNL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 28, 2002
Last modified: May 11, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.