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Reviewed, UniProtKB/Swiss-Prot P07108 (ACBP_HUMAN)

Last modified February 9, 2010. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Acyl-CoA-binding protein
      Short name=ACBP
Alternative name(s):
    Diazepam-binding inhibitor
      Short name=DBI
    Endozepine
      Short name=EP
Gene names
Name: DBI
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length87 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.

Subunit structure

Monomer.

Tissue specificity

Isoform 1 is ubiquitous, with a moderate expression level. Isoform 2 is ubiquitous with high level in liver and adipose tissue. Isoform 3 is ubiquitous with strong expression in adipose tissue and heart.

Sequence similarities

Belongs to the ACBP family.

Contains 1 ACB (acyl-CoA-binding) domain.

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Ontologies

Keywords
   Biological processTransport
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandLipid-binding
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionacyl-CoA binding

Inferred from electronic annotation. Source: InterPro

benzodiazepine receptor binding Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P07108-1)

Also known as: ACBP-1a; Short;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P07108-2)

Also known as: ACBP-1b; Long;

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MSQ → MWGDLWLLPPASANPGTGTE
Isoform 3 (identifier: P07108-3)

Also known as: ACBP-1c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MSQ → MPAF

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7 Ref.8
Chain2 – 8786Acyl-CoA-binding protein
PRO_0000214004

Regions

Domain2 – 8786ACB

Amino acid modifications

Modified residue21N-acetylserine Ref.7
Modified residue81N6-acetyllysine Ref.13
Modified residue191N6-acetyllysine Ref.13
Modified residue291Phosphotyrosine Ref.11
Modified residue551N6-acetyllysine Ref.13
Modified residue771N6-acetyllysine Ref.13

Natural variations

Alternative sequence1 – 33MSQ → MWGDLWLLPPASANPGTGTE in isoform 2.
VSP_000068
Alternative sequence1 – 33MSQ → MPAF in isoform 3.
VSP_038680
Natural variant391D → N: dbSNP rs8192504.
VAR_048160
Natural variant711M → V: dbSNP rs8192506.
VAR_048161
Natural variant861G → R: dbSNP rs8192507.
VAR_048162

Secondary structure

1 ........... 87
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ACBP-1a) (Short) [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B343A309F1B1AE28

FASTA8710,044
        10         20         30         40         50         60 
MSQAEFEKAA EEVRHLKTKP SDEEMLFIYG HYKQATVGDI NTERPGMLDF TGKAKWDAWN 

        70         80 
ELKGTSKEDA MKAYINKVEE LKKKYGI

« Hide

Isoform 2 (ACBP-1b) (Long).

Checksum: 54D0238ABE40BCD2
Show »

FASTA10411,793
Isoform 3 (ACBP-1c).

Checksum: F4FFCEBA6D9D4330
Show »

FASTA8810,145

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References

« Hide 'large scale' references
[1]"Cloning and expression of cDNA for human diazepam binding inhibitor, a natural ligand of an allosteric regulatory site of the gamma-aminobutyric acid type A receptor."
Gray P.W., Glaister D., Seeburg P.H., Guidotti A., Costa E.
Proc. Natl. Acad. Sci. U.S.A. 83:7547-7551(1986) [PubMed: 3020548] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Bovine and human cDNA sequences encoding a putative benzodiazepine receptor ligand."
Webb N.R., Rose T.M., Malik N., Marquardt H., Shoyab M., Todaro G.J., Lee D.C.
DNA 6:71-79(1987) [PubMed: 2881742] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Identification of new acyl-CoA binding protein transcripts in human and mouse."
Nitz I., Doering F., Schrezenmeir J., Burwinkel B.
Int. J. Biochem. Cell Biol. 37:2395-2405(2005) [PubMed: 16055366] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed: 15815621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.
[7]"Complete amino acid sequences of bovine and human endozepines. Homology with rat diazepam binding inhibitor."
Marquardt H., Todaro G.J., Shoyab M.
J. Biol. Chem. 261:9727-9731(1986) [PubMed: 3525533] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-87 (ISOFORM 1), ACETYLATION AT SER-2.
Tissue: Brain.
[8]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-14 (ISOFORM 1).
Tissue: Platelet.
[9]"Purification and analysis of growth regulating proteins secreted by a human melanoma cell line."
Apfel R., Lottspeich F., Hoppe J., Behl C., Duerr G., Bogdahn U.
Melanoma Res. 2:327-336(1992) [PubMed: 1292782] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-30 AND 72-87.
[10]"The characterization of two diazepam binding inhibitor (DBI) transcripts in humans."
Kolmer M., Rovio A., Alho H.
Biochem. J. 306:327-330(1995) [PubMed: 7534063] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[11]"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer."
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. expand/collapse author list , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.
Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-29, MASS SPECTROMETRY.
[12]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[13]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed: 19608861] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-8; LYS-19; LYS-55 AND LYS-77, MASS SPECTROMETRY.
[14]"High resolution crystal structures of unliganded and liganded human liver ACBP reveal a new mode of binding for the acyl-CoA ligand."
Taskinen J.P., van Aalten D.M., Knudsen J., Wierenga R.K.
Proteins 66:229-238(2007) [PubMed: 17044054] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M14200 mRNA. Translation: AAA52171.1.
M15887 mRNA. Translation: AAA35788.1.
CR456956 mRNA. Translation: CAG33237.1.
AC016736 Genomic DNA. Translation: AAY14873.1.
BC006466 mRNA. No translation available.
BC062996 mRNA. Translation: AAH62996.1.
AM000001 mRNA. Translation: CAJ00736.1.
IPIIPI00010182.
IPI00218836.
PIRNZHU. B26448.
RefSeqNP_001073331.1.
NP_065438.1.
UniGeneHs.78888

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CB8X-ray1.40A/B1-87[»]
2FJ9X-ray1.60A2-87[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP07108. 1 interaction.
STRINGP07108.

PTM databases

PhosphoSiteP07108.

Proteomic databases

PRIDEP07108.

Genome annotation databases

EnsemblENST00000355857; ENSP00000348116; ENSG00000155368; Homo sapiens. [Genome view]
ENST00000393103; ENSP00000376815; ENSG00000155368; Homo sapiens. [Genome view]
GeneID1622.
KEGGhsa:1622.
UCSCuc002tlv.1. human.
uc002tlw.1. human.

Organism-specific databases

CTD1622.
GeneCardsGC02P119840.
H-InvDBHIX0029917.
HGNCHGNC:2690. DBI.
HPACAB008595.
MIM125950. gene.
PharmGKBPA27158.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG20762.
HOVERGENP07108.
OMAERPDNAT.
OrthoDBEOG96Q9C3.
PhylomeDBP07108.

Gene expression databases

ArrayExpressP07108.
BgeeP07108.
CleanExHS_DBI.
GenevestigatorP07108.
GermOnlineENSG00000155368. Homo sapiens.

Family and domain databases

InterProIPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA_bd_prot_3-hlx.
[Graphical view]
Gene3DG3DSA:1.20.80.10. ACBP. 1 hit.
PANTHERPTHR23310. ACBP. 1 hit.
PfamPF00887. ACBP. 1 hit.
[Graphical view]
PRINTSPR00689. ACOABINDINGP.
PROSITEPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio6658.
SOURCESearch...

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Entry information

Entry nameACBP_HUMAN
AccessionPrimary (citable) accession number: P07108
Secondary accession number(s): P08869 expand/collapse secondary AC list , Q4VWZ6, Q53SQ7, Q6IB48, Q9UCI8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: February 9, 2010
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 2: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

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Index of human polymorphisms and disease mutations

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Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents