P07107 (ACBP_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-CoA-binding protein
Short name=ACBP

Alternative name(s):
Diazepam-binding inhibitor
Short name=DBI
Endozepine
Short name=EP

Gene names

Name:

DBI

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Subunit structure	Monomer.
Sequence similarities	Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.

Ontologies

Keywords
Biological process	Transport
Ligand	Lipid-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fatty-acyl-CoA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3 Ref.4 Ref.5

Chain

2 – 87

86

Acyl-CoA-binding protein

PRO_0000214001

Regions

Domain

2 – 87

86

ACB

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.3

Modified residue

8

1

N6-acetyllysine By similarity

Modified residue

19

1

N6-acetyllysine By similarity

Modified residue

29

1

Phosphotyrosine By similarity

Modified residue

55

1

N6-acetyllysine By similarity

Modified residue

77

1

N6-acetyllysine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

87

Helix Strand Turn

Details...

Helix

3 – 12

10

Helix

13 – 15

3

Helix

22 – 36

15

Helix

50 – 61

12

Turn

62 – 64

3

Helix

67 – 85

19

Sequences

	Sequence		Length	Mass (Da)
	P07107 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B2922635F18CA0D8	FASTA	87	10,044
	10 20 30 40 50 60 MSQAEFDKAA EEVKHLKTKP ADEEMLFIYS HYKQATVGDI NTERPGMLDF KGKAKWDAWN 70 80 ELKGTSKEDA MKAYIDKVEE LKKKYGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine and human cDNA sequences encoding a putative benzodiazepine receptor ligand." Webb N.R., Rose T.M., Malik N., Marquardt H., Shoyab M., Todaro G.J., Lee D.C. DNA 6:71-79(1987) [PubMed: 2881742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[3]	"Complete amino acid sequences of bovine and human endozepines. Homology with rat diazepam binding inhibitor." Marquardt H., Todaro G.J., Shoyab M. J. Biol. Chem. 261:9727-9731(1986) [PubMed: 3525533] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-87, ACETYLATION AT SER-2. Tissue: Brain.
[4]	"Amino acid sequence of acyl-CoA-binding protein from cow liver." Mikkelsen J., Hoejrup P., Nielsen P.F., Roepstorff P., Knudsen J. Biochem. J. 245:857-861(1987) [PubMed: 3663196] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-87. Tissue: Liver.
[5]	"Purification and characterization of variants of acyl-CoA-binding protein in the bovine liver." Jensen M.S., Hoejrup P., Rasmussen J.T., Knudsen J. Biochem. J. 284:809-812(1992) [PubMed: 1622397] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-87. Tissue: Liver.
[6]	"The secondary structure in solution of acyl-coenzyme A binding protein from bovine liver using 1H nuclear magnetic resonance spectroscopy." Andersen K.V., Ludvigsen S., Mandrup S., Knudsen J., Poulsen F.M. Biochemistry 30:10654-10663(1991) [PubMed: 1931985] [Abstract] Cited for: STRUCTURE BY NMR.
[7]	"Three-dimensional structure in solution of acyl-coenzyme A binding protein from bovine liver." Andersen K.V., Poulsen F.M. J. Mol. Biol. 226:1131-1141(1992) [PubMed: 1518047] [Abstract] Cited for: STRUCTURE BY NMR.
[8]	"The three-dimensional structure of acyl-coenzyme A binding protein from bovine liver: structural refinement using heteronuclear multidimensional NMR spectroscopy." Andersen K.V., Poulsen F.M. J. Biomol. NMR 3:271-284(1993) [PubMed: 8358232] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15886 mRNA. Translation: AAA30495.1.
BC114181 mRNA. Translation: AAI14182.1.

IPI

IPI00695419.

PIR

NZBO. A26448.

RefSeq

NP_001106792.1. NM_001113321.1.

UniGene

Bt.46917.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ACA	NMR	-	A	2-86	[»]
1HB6	X-ray	2.00	A	2-86	[»]
1HB8	X-ray	2.00	A/B/C	2-86	[»]
1NTI	NMR	-	A	2-86	[»]
1NVL	NMR	-	A	2-87	[»]
2ABD	NMR	-	A	2-87	[»]

ProteinModelPortal

P07107.

SMR

P07107. Positions 2-87.

ModBase

Search...

Protein-protein interaction databases

STRING

P07107.

Proteomic databases

PRIDE

P07107.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000012523; ENSBTAP00000012523; ENSBTAG00000009517.

GeneID

768330.

KEGG

bta:768330.

Organism-specific databases

CTD

1622.

Phylogenomic databases

eggNOG

maNOG21202.

GeneTree

ENSGT00390000005957.

HOVERGEN

HBG000398.

InParanoid

P07107.

OMA

WKELEGT.

OrthoDB

EOG4GF3GS.

PhylomeDB

P07107.

Family and domain databases

InterPro

IPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
[Graphical view]

Gene3D

G3DSA:1.20.80.10. ACBP. 1 hit.

KO

K08762.

Pfam

PF00887. ACBP. 1 hit.
[Graphical view]

PRINTS

PR00689. ACOABINDINGP.

SUPFAM

SSF47027. ACBP. 1 hit.

PROSITE

PS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACBP_BOVIN

Accession

Primary (citable) accession number: P07107
Secondary accession number(s): Q29RG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program