##gff-version 3 P07102 UniProtKB Signal peptide 1 22 . . . Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:2168385,ECO:0000269|PubMed:8387749,ECO:0000269|Ref.8;Dbxref=PMID:2168385,PMID:8387749 P07102 UniProtKB Chain 23 432 . . . ID=PRO_0000023947;Note=Phytase AppA P07102 UniProtKB Active site 39 39 . . . Note=Nucleophile;Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:10655611,ECO:0000305|PubMed:1429631;Dbxref=PMID:10655611,PMID:1429631 P07102 UniProtKB Active site 326 326 . . . Note=Proton donor;Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:10655611,ECO:0000305|PubMed:8407904;Dbxref=PMID:10655611,PMID:8407904 P07102 UniProtKB Binding site 38 38 . . . Ontology_term=ECO:0000269,ECO:0000305,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:10655611,ECO:0000305|Ref.18,ECO:0007744|PDB:1DKP,ECO:0007744|PDB:1DKQ;Dbxref=PMID:10655611 P07102 UniProtKB Binding site 42 46 . . . Ontology_term=ECO:0000269,ECO:0000305,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:10655611,ECO:0000305|Ref.18,ECO:0007744|PDB:1DKP,ECO:0007744|PDB:1DKQ;Dbxref=PMID:10655611 P07102 UniProtKB Binding site 114 114 . . . Ontology_term=ECO:0000269,ECO:0000305,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:10655611,ECO:0000305|Ref.18,ECO:0007744|PDB:1DKP,ECO:0007744|PDB:1DKQ;Dbxref=PMID:10655611 P07102 UniProtKB Binding site 289 289 . . . Ontology_term=ECO:0000269,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:10655611,ECO:0007744|PDB:1DKP,ECO:0007744|PDB:1DKQ;Dbxref=PMID:10655611 P07102 UniProtKB Binding site 325 327 . . . Ontology_term=ECO:0000269,ECO:0000305,ECO:0007744,ECO:0007744;evidence=ECO:0000269|PubMed:10655611,ECO:0000305|Ref.18,ECO:0007744|PDB:1DKP,ECO:0007744|PDB:1DKQ;Dbxref=PMID:10655611 P07102 UniProtKB Disulfide bond 99 130 . . . Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10655611,ECO:0000269|PubMed:15642731,ECO:0000269|Ref.18;Dbxref=PMID:10655611,PMID:15642731 P07102 UniProtKB Disulfide bond 155 430 . . . Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10655611,ECO:0000269|PubMed:15642731,ECO:0000269|Ref.18;Dbxref=PMID:10655611,PMID:15642731 P07102 UniProtKB Disulfide bond 200 210 . . . Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10655611,ECO:0000269|PubMed:15642731;Dbxref=PMID:10655611,PMID:15642731 P07102 UniProtKB Disulfide bond 404 413 . . . Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10655611,ECO:0000269|PubMed:15642731,ECO:0000269|Ref.18;Dbxref=PMID:10655611,PMID:15642731 P07102 UniProtKB Mutagenesis 38 38 . . . Note=Loss of activity with pNPP and phytate as substrates. R->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:1429631,ECO:0000269|PubMed:30712472;Dbxref=PMID:1429631,PMID:30712472 P07102 UniProtKB Mutagenesis 38 38 . . . Note=Loss of activity with phytate as substrate. R->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 39 39 . . . Note=Loss of activity with phytate as substrate. H->A%2CG;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10655611,ECO:0000269|PubMed:30712472;Dbxref=PMID:10655611,PMID:30712472 P07102 UniProtKB Mutagenesis 39 39 . . . Note=Loss of activity with pNPP as substrate. H->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1429631;Dbxref=PMID:1429631 P07102 UniProtKB Mutagenesis 40 40 . . . Note=Retains 50%25 of wild-type activity with phytate as substrate. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 41 41 . . . Note=Retains 70%25 of wild-type activity with phytate as substrate. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 41 41 . . . Note=Retains less than 10%25 of wild-type activity with phytate as substrate. V->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 42 42 . . . Note=Shows residual activity with pNPP as substrate. Loss of activity with phytate as substrate. R->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:1429631,ECO:0000269|PubMed:30712472;Dbxref=PMID:1429631,PMID:30712472 P07102 UniProtKB Mutagenesis 42 42 . . . Note=Loss of activity with phytate as substrate. R->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 43 43 . . . Note=Retains 70%25 of wild-type activity with phytate as substrate. A->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 44 44 . . . Note=Retains 70%25 of wild-type activity with phytate as substrate. P->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 44 44 . . . Note=Retains 50%25 of wild-type activity with phytate as substrate. P->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 45 45 . . . Note=Retains 20%25 of wild-type activity with phytate as substrate. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 45 45 . . . Note=Retains less than 10%25 of wild-type activity with phytate as substrate. T->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 46 46 . . . Note=Retains 40%25 of wild-type activity with phytate as substrate. K->A%2CG;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 47 47 . . . Note=Retains 80%25 of wild-type activity with phytate as substrate. A->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 48 48 . . . Note=Retains 80%25 of wild-type activity with phytate as substrate. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 48 48 . . . Note=Retains 90%25 of wild-type activity with phytate as substrate. T->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30712472;Dbxref=PMID:30712472 P07102 UniProtKB Mutagenesis 85 85 . . . Note=Does not affect activity with pNPP as substrate. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1429631;Dbxref=PMID:1429631 P07102 UniProtKB Mutagenesis 99 99 . . . Note=Retains less than 10%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 114 114 . . . Note=Shows residual activity with pNPP as substrate. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1429631;Dbxref=PMID:1429631 P07102 UniProtKB Mutagenesis 130 130 . . . Note=Retains less than 10%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 155 155 . . . Note=Retains less than 10%25 of wild-type activity. Does not require DsbC for its remaining activity%3B when associated with S-430. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 200 200 . . . Note=Retains 70%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 210 210 . . . Note=Retains 35%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 325 325 . . . Note=Shows residual activity with pNPP as substrate. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1429631;Dbxref=PMID:1429631 P07102 UniProtKB Mutagenesis 326 326 . . . Note=28-fold decrease in Vmax with pNPP as substrate. 1600-fold decrease in Vmax with fructose 1%2C6-bisphosphate as substrate. Does not affect the Km values of the substrates pNPP%2C fructose 1%2C6-diphosphate and tripolyphosphate. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8407904;Dbxref=PMID:8407904 P07102 UniProtKB Mutagenesis 326 326 . . . Note=40-fold decrease in Vmax with pNPP as substrate. 460-fold decrease in Vmax with fructose 1%2C6-bisphosphate as substrate. Does not affect the Km values of the substrates pNPP%2C fructose 1%2C6-diphosphate and tripolyphosphate. D->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8407904;Dbxref=PMID:8407904 P07102 UniProtKB Mutagenesis 404 404 . . . Note=Retains less than 10%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 413 413 . . . Note=Retains less than 10%25 of wild-type activity. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Mutagenesis 430 430 . . . Note=Retains less than 10%25 of wild-type activity. Does not require DsbC for its remaining activity%3B when associated with S-155. C->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15642731;Dbxref=PMID:15642731 P07102 UniProtKB Sequence conflict 51 66 . . . Note=MQDVTPDAWPTWPVKL->NAGCHPRRMANLAGKT;Ontology_term=ECO:0000305;evidence=ECO:0000305 P07102 UniProtKB Sequence conflict 75 76 . . . Note=EL->DV;Ontology_term=ECO:0000305;evidence=ECO:0000305 P07102 UniProtKB Sequence conflict 112 112 . . . Note=D->S;Ontology_term=ECO:0000305;evidence=ECO:0000305 P07102 UniProtKB Beta strand 28 38 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 49 53 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 63 65 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 71 90 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 96 98 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 104 109 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 113 126 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 145 147 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 149 152 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 159 169 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Turn 170 172 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1DKQ P07102 UniProtKB Helix 174 179 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 182 192 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 194 196 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 198 205 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 212 215 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 220 223 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 226 229 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 231 248 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 254 257 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 262 279 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 283 301 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 310 312 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 314 316 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 318 324 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 326 336 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 345 349 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1DKQ P07102 UniProtKB Beta strand 354 362 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Turn 363 366 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 367 376 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 379 383 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 390 392 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 395 398 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Beta strand 405 407 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 415 425 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T P07102 UniProtKB Helix 428 430 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7Z2T