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Protein

Periplasmic AppA protein

Gene

appA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.
Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Substrate1
Active sitei39Nucleophile1
Binding sitei114Substrate1
Binding sitei289Substrate1
Active sitei326Proton donor1

GO - Molecular functioni

  • 4-phytase activity Source: EcoCyc
  • acid phosphatase activity Source: EcoCyc

GO - Biological processi

  • cellular response to anoxia Source: EcoCyc
  • cellular response to phosphate starvation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:APPA-MONOMER.
ECOL316407:JW0963-MONOMER.
MetaCyc:APPA-MONOMER.
BRENDAi3.1.3.26. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic AppA protein
Including the following 2 domains:
Phosphoanhydride phosphohydrolase (EC:3.1.3.2)
Alternative name(s):
pH 2.5 acid phosphatase
Short name:
AP
4-phytase (EC:3.1.3.26)
Gene namesi
Name:appA
Ordered Locus Names:b0980, JW0963
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10049. appA.

Subcellular locationi

GO - Cellular componenti

  • periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 223 PublicationsAdd BLAST22
ChainiPRO_000002394723 – 432Periplasmic AppA proteinAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi99 ↔ 130
Disulfide bondi155 ↔ 430
Disulfide bondi200 ↔ 210
Disulfide bondi404 ↔ 413

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP07102.
PRIDEiP07102.

2D gel databases

SWISS-2DPAGEP07102.

Expressioni

Inductioni

In addition to cAMP-mediated control, this enzyme is induced when bacterial cultures reach stationary phase; its synthesis is triggered by phosphate starvation or a shift from aerobic to anaerobic conditions.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259354. 14 interactors.
IntActiP07102. 3 interactors.
STRINGi511145.b0980.

Structurei

Secondary structure

1432
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 38Combined sources12
Helixi49 – 53Combined sources5
Beta strandi63 – 65Combined sources3
Helixi71 – 90Combined sources20
Beta strandi96 – 98Combined sources3
Turni102 – 104Combined sources3
Beta strandi105 – 109Combined sources5
Helixi113 – 126Combined sources14
Helixi145 – 147Combined sources3
Turni149 – 153Combined sources5
Helixi159 – 169Combined sources11
Turni170 – 172Combined sources3
Helixi174 – 179Combined sources6
Helixi182 – 192Combined sources11
Helixi194 – 196Combined sources3
Helixi198 – 201Combined sources4
Turni204 – 207Combined sources4
Helixi212 – 215Combined sources4
Beta strandi220 – 222Combined sources3
Beta strandi227 – 229Combined sources3
Helixi232 – 249Combined sources18
Helixi254 – 257Combined sources4
Helixi262 – 279Combined sources18
Helixi283 – 289Combined sources7
Helixi291 – 301Combined sources11
Helixi310 – 312Combined sources3
Beta strandi314 – 316Combined sources3
Beta strandi318 – 324Combined sources7
Helixi326 – 336Combined sources11
Beta strandi345 – 349Combined sources5
Beta strandi354 – 362Combined sources9
Turni363 – 366Combined sources4
Beta strandi367 – 376Combined sources10
Helixi379 – 383Combined sources5
Beta strandi390 – 392Combined sources3
Beta strandi395 – 399Combined sources5
Beta strandi405 – 407Combined sources3
Helixi415 – 425Combined sources11
Helixi428 – 430Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DKLX-ray2.30A/B23-432[»]
1DKMX-ray2.25A23-432[»]
1DKNX-ray2.40A23-432[»]
1DKOX-ray2.38A23-432[»]
1DKPX-ray2.28A23-432[»]
1DKQX-ray2.05A23-432[»]
4TSRX-ray2.07A23-432[»]
ProteinModelPortaliP07102.
SMRiP07102.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07102.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 46Substrate binding5
Regioni325 – 327Substrate binding3

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D6F. Bacteria.
ENOG410XRK8. LUCA.
HOGENOMiHOG000118851.
InParanoidiP07102.
KOiK01093.
OMAiKSCSLTQ.
PhylomeDBiP07102.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 3 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07102-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAILIPFLS LLIPLTPQSA FAQSEPELKL ESVVIVSRHG VRAPTKATQL
60 70 80 90 100
MQDVTPDAWP TWPVKLGWLT PRGGELIAYL GHYQRQRLVA DGLLAKKGCP
110 120 130 140 150
QSGQVAIIAD VDERTRKTGE AFAAGLAPDC AITVHTQADT SSPDPLFNPL
160 170 180 190 200
KTGVCQLDNA NVTDAILSRA GGSIADFTGH RQTAFRELER VLNFPQSNLC
210 220 230 240 250
LKREKQDESC SLTQALPSEL KVSADNVSLT GAVSLASMLT EIFLLQQAQG
260 270 280 290 300
MPEPGWGRIT DSHQWNTLLS LHNAQFYLLQ RTPEVARSRA TPLLDLIKTA
310 320 330 340 350
LTPHPPQKQA YGVTLPTSVL FIAGHDTNLA NLGGALELNW TLPGQPDNTP
360 370 380 390 400
PGGELVFERW RRLSDNSQWI QVSLVFQTLQ QMRDKTPLSL NTPPGEVKLT
410 420 430
LAGCEERNAQ GMCSLAGFTQ IVNEARIPAC SL
Length:432
Mass (Da):47,057
Last modified:August 1, 1991 - v2
Checksum:i6510C6C579177F11
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51 – 66MQDVT…WPVKL → NAGCHPRRMANLAGKT in CAA29031 (PubMed:3038201).CuratedAdd BLAST16
Sequence conflicti75 – 76EL → DV in CAA29031 (PubMed:3038201).Curated2
Sequence conflicti112D → S in CAA29031 (PubMed:3038201).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58708 Genomic DNA. Translation: AAA72086.1.
U00096 Genomic DNA. Translation: AAC74065.1.
AP009048 Genomic DNA. Translation: BAA35745.1.
X05471 Genomic DNA. Translation: CAA29031.1.
S63811 Genomic DNA. Translation: AAB20286.1.
PIRiB36733.
RefSeqiNP_415500.1. NC_000913.3.
WP_001300464.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74065; AAC74065; b0980.
BAA35745; BAA35745; BAA35745.
GeneIDi946206.
KEGGiecj:JW0963.
eco:b0980.
PATRICi32117183. VBIEscCol129921_1015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58708 Genomic DNA. Translation: AAA72086.1.
U00096 Genomic DNA. Translation: AAC74065.1.
AP009048 Genomic DNA. Translation: BAA35745.1.
X05471 Genomic DNA. Translation: CAA29031.1.
S63811 Genomic DNA. Translation: AAB20286.1.
PIRiB36733.
RefSeqiNP_415500.1. NC_000913.3.
WP_001300464.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DKLX-ray2.30A/B23-432[»]
1DKMX-ray2.25A23-432[»]
1DKNX-ray2.40A23-432[»]
1DKOX-ray2.38A23-432[»]
1DKPX-ray2.28A23-432[»]
1DKQX-ray2.05A23-432[»]
4TSRX-ray2.07A23-432[»]
ProteinModelPortaliP07102.
SMRiP07102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259354. 14 interactors.
IntActiP07102. 3 interactors.
STRINGi511145.b0980.

2D gel databases

SWISS-2DPAGEP07102.

Proteomic databases

PaxDbiP07102.
PRIDEiP07102.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74065; AAC74065; b0980.
BAA35745; BAA35745; BAA35745.
GeneIDi946206.
KEGGiecj:JW0963.
eco:b0980.
PATRICi32117183. VBIEscCol129921_1015.

Organism-specific databases

EchoBASEiEB0047.
EcoGeneiEG10049. appA.

Phylogenomic databases

eggNOGiENOG4105D6F. Bacteria.
ENOG410XRK8. LUCA.
HOGENOMiHOG000118851.
InParanoidiP07102.
KOiK01093.
OMAiKSCSLTQ.
PhylomeDBiP07102.

Enzyme and pathway databases

BioCyciEcoCyc:APPA-MONOMER.
ECOL316407:JW0963-MONOMER.
MetaCyc:APPA-MONOMER.
BRENDAi3.1.3.26. 2026.

Miscellaneous databases

EvolutionaryTraceiP07102.
PROiP07102.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 3 hits.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPA_ECOLI
AccessioniPrimary (citable) accession number: P07102
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.