P07098 (LIPG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastric triacylglycerol lipase
Short name=GL
Short name=Gastric lipase
EC=3.1.1.3

Gene names

Name:

LIPF

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Sequence caution	The sequence CAA29414.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride metabolic process Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Non-traceable author statement Ref.7. Source: UniProtKB retinyl-palmitate esterase activity Inferred from electronic annotation. Source: EC triglyceride lipase activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.7

Chain

20 – 398

379

Gastric triacylglycerol lipase

PRO_0000017766

Sites

Active site

172

1

Nucleophile

Active site

343

1

Charge relay system

Active site

372

1

Charge relay system

Amino acid modifications

Glycosylation

34

1

N-linked (GlcNAc...)

Glycosylation

99

1

N-linked (GlcNAc...)

Glycosylation

271

1

N-linked (GlcNAc...)

Glycosylation

327

1

N-linked (GlcNAc...)

Disulfide bond

246 ↔ 255

Natural variations

Natural variant

161

1

T → A. Ref.3 Ref.6
Corresponds to variant rs814628 [ dbSNP | Ensembl ].

VAR_011947

Natural variant

224

1

F → I. Ref.3
Corresponds to variant rs6586145 [ dbSNP | Ensembl ].

VAR_020565

Natural variant

348

1

P → T. Ref.3
Corresponds to variant rs17333991 [ dbSNP | Ensembl ].

VAR_020566

Secondary structure

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398

Helix Strand Turn

Details...

Helix

29 – 32

4

Helix

35 – 41

7

Beta strand

47 – 52

6

Beta strand

56 – 64

9

Beta strand

79 – 83

5

Helix

90 – 94

5

Helix

102 – 108

7

Beta strand

112 – 115

4

Beta strand

127 – 130

4

Helix

136 – 138

3

Helix

142 – 147

6

Helix

149 – 161

13

Beta strand

166 – 171

6

Helix

173 – 184

12

Helix

186 – 189

4

Beta strand

192 – 199

8

Helix

210 – 216

7

Helix

219 – 226

8

Beta strand

228 – 230

3

Turn

234 – 239

6

Helix

241 – 249

9

Helix

252 – 255

4

Helix

258 – 263

6

Helix

272 – 274

3

Helix

275 – 279

5

Helix

288 – 300

13

Helix

311 – 318

8

Beta strand

319 – 322

4

Helix

328 – 330

3

Beta strand

335 – 340

6

Beta strand

344 – 346

3

Helix

348 – 357

10

Beta strand

361 – 367

7

Helix

374 – 377

4

Helix

381 – 384

4

Helix

386 – 393

8

Turn

394 – 397

4

Sequences

	Sequence		Length	Mass (Da)
	P07098 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: CD3EE1621C014F0F	FASTA	398	45,238
	10 20 30 40 50 60 MWLLLTMASL ISVLGTTHGL FGKLHPGSPE VTMNISQMIT YWGYPNEEYE VVTEDGYILE 70 80 90 100 110 120 VNRIPYGKKN SGNTGQRPVV FLQHGLLASA TNWISNLPNN SLAFILADAG YDVWLGNSRG 130 140 150 160 170 180 NTWARRNLYY SPDSVEFWAF SFDEMAKYDL PATIDFIVKK TGQKQLHYVG HSQGTTIGFI 190 200 210 220 230 240 AFSTNPSLAK RIKTFYALAP VATVKYTKSL INKLRFVPQS LFKFIFGDKI FYPHNFFDQF 250 260 270 280 290 300 LATEVCSREM LNLLCSNALF IICGFDSKNF NTSRLDVYLS HNPAGTSVQN MFHWTQAVKS 310 320 330 340 350 360 GKFQAYDWGS PVQNRMHYDQ SQPPYYNVTA MNVPIAVWNG GKDLLADPQD VGLLLPKLPN 370 380 390 LIYHKEIPFY NHLDFIWAMD APQEVYNDIV SMISEDKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a human gastric lipase and expression of the enzyme in yeast." Bodmer M.W., Angal S., Yarranton G.T., Harris T.J.R., Lyons A., King D.J., Pieroni G., Riviere C., Verger R., Lowe P.A. Biochim. Biophys. Acta 909:237-244(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Stomach.
[3]	NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-161; ILE-224 AND THR-348.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-161.
[7]	"Human gastric lipase. The N-terminal tetrapeptide is essential for lipid binding and lipase activity." Bernbaeck S., Blaeckberg L. Eur. J. Biochem. 182:495-499(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-45.
[8]	"Crystal structure of human gastric lipase and model of lysosomal acid lipase, two lipolytic enzymes of medical interest." Roussel A., Canaan S., Egloff M.P., Riviere M., Dupuis L., Verger R., Cambillau C. J. Biol. Chem. 274:16995-17002(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X05997 mRNA. Translation: CAA29413.1.
X05997 mRNA. Translation: CAA29414.1. Different initiation.
AK312940 mRNA. Translation: BAG35782.1.
AY631869 Genomic DNA. Translation: AAT38115.1.
AL358532 Genomic DNA. Translation: CAH71057.1.
CH471066 Genomic DNA. Translation: EAW50162.1.
BC112272 mRNA. Translation: AAI12273.1.
BC113711 mRNA. Translation: AAI13712.1.

IPI

IPI00009893.

PIR

S07145.

RefSeq

NP_004181.1. NM_004190.3.

UniGene

Hs.523130.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HLG	X-ray	3.00	A/B	28-398	[»]

ProteinModelPortal

P07098.

ModBase

Search...

Protein-protein interaction databases

IntAct

P07098. 1 interaction.

STRING

9606.ENSP00000238983.

PTM databases

PhosphoSite

P07098.

Polymorphism databases

DMDM

126306.

Proteomic databases

PaxDb

P07098.

PRIDE

P07098.

Protocols and materials databases

DNASU

8513.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000238983; ENSP00000238983; ENSG00000182333.

GeneID

8513.

KEGG

hsa:8513.

UCSC

uc001kfg.2. human.

Organism-specific databases

CTD

8513.

GeneCards

GC10P090415.

HGNC

HGNC:6622. LIPF.

HPA

HPA045930.

MIM

601980. gene.

neXtProt

NX_P07098.

PharmGKB

PA30394.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0596.

HOVERGEN

HBG006265.

InParanoid

P07098.

KO

K14452.

OrthoDB

EOG43TZVJ.

PhylomeDB

P07098.

Gene expression databases

ArrayExpress

P07098.

Bgee

P07098.

CleanEx

HS_LIPF.

Genevestigator

P07098.

GermOnline

ENSG00000182333. Homo sapiens.

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR006693. AB_hydrolase_lipase.
IPR025483. Lipase_euk.
[Graphical view]

Pfam

PF04083. Abhydro_lipase. 1 hit.
PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000862. Steryl_ester_lip. 1 hit.

PROSITE

PS00120. LIPASE_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1796.

ChiTaRS

LIPF. human.

EvolutionaryTrace

P07098.

GenomeRNAi

8513.

NextBio

31864.

PMAP-CutDB

P07098.

SOURCE

Search...

Entry information

Entry name

LIPG_HUMAN

Accession

Primary (citable) accession number: P07098
Secondary accession number(s): Q2M1P6, Q5VXI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 140 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.