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Protein

Glia-derived nexin

Gene

Serpine2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei364 – 3652Reactive bondSequence Analysis

GO - Molecular functioni

  1. heparin binding Source: RGD
  2. serine-type endopeptidase inhibitor activity Source: RGD

GO - Biological processi

  1. dense core granule biogenesis Source: RGD
  2. embryo implantation Source: RGD
  3. negative regulation of blood coagulation Source: BHF-UCL
  4. negative regulation of endopeptidase activity Source: GO_Central
  5. negative regulation of proteolysis Source: BHF-UCL
  6. positive regulation of neuron projection development Source: RGD
  7. response to radiation Source: RGD
  8. secretory granule organization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Heparin-binding

Protein family/group databases

MEROPSiI04.021.

Names & Taxonomyi

Protein namesi
Recommended name:
Glia-derived nexin
Short name:
GDN
Alternative name(s):
Peptidase inhibitor 7
Short name:
PI-7
Protease nexin 1
Short name:
PN-1
Protease nexin I
Serpin E2
Gene namesi
Name:Serpine2
Synonyms:Pi7, Pn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3748. Serpine2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
  2. membrane Source: RGD
  3. neuronal cell body Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 397378Glia-derived nexinPRO_0000032506Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP07092.
PRIDEiP07092.

PTM databases

PhosphoSiteiP07092.

Expressioni

Gene expression databases

GenevestigatoriP07092.

Interactioni

Protein-protein interaction databases

BioGridi248020. 1 interaction.
IntActiP07092. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP07092.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP07092.
KOiK16643.
PhylomeDBiP07092.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07092-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNWHFPFFIL TTVTLSSVYS QLNSLSLEEL GSDTGIQVFN QIIKSQPHEN
60 70 80 90 100
VVISPHGIAS ILGMLQLGAD GRTKKQLSTV MRYNVNGVGK VLKKINKAIV
110 120 130 140 150
SKKNKDIVTV ANAVFVRNGF KVEVPFAARN KEVFQCEVQS VNFQDPASAC
160 170 180 190 200
DAINFWVKNE TRGMIDNLLS PNLIDSALTK LVLVNAVYFK GLWKSRFQPE
210 220 230 240 250
NTKKRTFVAG DGKSYQVPML AQLSVFRSGS TKTPNGLWYN FIELPYHGES
260 270 280 290 300
ISMLIALPTE SSTPLSAIIP HISTKTINSW MNTMVPKRMQ LVLPKFTALA
310 320 330 340 350
QTDLKEPLKA LGITEMFEPS KANFAKITRS ESLHVSHILQ KAKIEVSEDG
360 370 380 390
TKAAVVTTAI LIARSSPPWF IVDRPFLFCI RHNPTGAILF LGQVNKP
Length:397
Mass (Da):44,063
Last modified:April 1, 1988 - v1
Checksum:i11EF0790C7297646
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17784 mRNA. Translation: AAA41209.1.
PIRiB27496.
RefSeqiNP_062070.1. NM_019197.1.
UniGeneiRn.2271.

Genome annotation databases

GeneIDi29366.
KEGGirno:29366.
UCSCiRGD:3748. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17784 mRNA. Translation: AAA41209.1.
PIRiB27496.
RefSeqiNP_062070.1. NM_019197.1.
UniGeneiRn.2271.

3D structure databases

ProteinModelPortaliP07092.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248020. 1 interaction.
IntActiP07092. 1 interaction.

Protein family/group databases

MEROPSiI04.021.

PTM databases

PhosphoSiteiP07092.

Proteomic databases

PaxDbiP07092.
PRIDEiP07092.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29366.
KEGGirno:29366.
UCSCiRGD:3748. rat.

Organism-specific databases

CTDi5270.
RGDi3748. Serpine2.

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP07092.
KOiK16643.
PhylomeDBiP07092.

Miscellaneous databases

NextBioi608908.
PROiP07092.

Gene expression databases

GenevestigatoriP07092.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA sequence coding for a rat glia-derived nexin and its homology to members of the serpin superfamily."
    Sommer J., Gloor S.M., Rovelli G.F., Hofsteenge J., Nick H., Meier R., Monard D.
    Biochemistry 26:6407-6410(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the heparin-binding site of glia-derived nexin/protease nexin-1."
    Rovelli G., Stone S.R., Guidolin A., Sommer J., Monard D.
    Biochemistry 31:3542-3549(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 82-96; 165-178 AND 317-333, HEPARIN-BINDING.
  3. "Functional sites of glia-derived nexin (GDN): importance of the site reacting with the protease."
    Nick H., Hofsteenge J., Shaw E., Rovelli G., Monard D.
    Biochemistry 29:2417-2421(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 133-153 AND 347-397, FUNCTION.

Entry informationi

Entry nameiGDN_RAT
AccessioniPrimary (citable) accession number: P07092
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: February 4, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.