Anaerobic ribonucleoside-triphosphate reductase

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P07071 (NRDD_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Anaerobic ribonucleoside-triphosphate reductase
EC=1.17.4.2

Gene names

Name:	NRDD
Synonyms:	49.1, 55.11/55.13, SUNY

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H₂O = ribonucleoside triphosphate + thioredoxin.
Subunit structure	Tetramer consisting of 2 alpha (NrdD) and 2 beta (NrdG) subunits.
Induction	Probably by activating enzyme under anaerobic conditions by generation of an organic free radical. Exposure of this activated protein to oxygen may result in cleavage at the glycine residue harboring its organic radical with loss of the 25 C-terminal AA.
Sequence similarities	Belongs to the anaerobic ribonucleoside-triphosphate reductase family. Contains 1 glycine radical domain.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Oxidoreductase
PTM	Organic radical
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-triphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 605

605

Anaerobic ribonucleoside-triphosphate reductase

PRO_0000166686

Regions

Domain

482 – 605

124

Glycine radical

Sites

Metal binding

543

Zinc

Metal binding

546

Zinc

Metal binding

561

Zinc

Metal binding

564

Zinc

Amino acid modifications

Modified residue

580

Glycine radical

Secondary structure

605

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07071 [UniParc].

Last modified January 23, 2002. Version 4.
Checksum: C5F29CE03126800B
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FASTA

605

67,957

        10         20         30         40         50         60 
MTIEKEIEGL IHKTNKDLLN ENANKDSRVF PTQRDLMAGI VSKHIAKNMV PSFIMKAHES 

        70         80         90        100        110        120 
GIIHVHDIDY SPALPFTNCC LVDLKGMLEN GFKLGNAQIE TPKSIGVATA IMAQITAQVA 

       130        140        150        160        170        180 
SHQYGGTTFA NVDKVLSPYV KRTYAKHIED AEKWQIADAL NYAQSKTEKD VYDAFQAYEY 

       190        200        210        220        230        240 
EVNTLFSSNG QTPFVTITFG TGTDWTERMI QKAILKNRIK GLGRDGITPI FPKLVMFVEE 

       250        260        270        280        290        300 
GVNLYKDDPN YDIKQLALEC ASKRMYPDII SAKNNKAITG SSVPVSPMGC RSFLSVWKDS 

       310        320        330        340        350        360 
TGNEILDGRN NLGVVTLNLP RIALDSYIGT QFNEQKFVEL FNERMDLCFE ALMCRISSLK 

       370        380        390        400        410        420 
GVKATVAPIL YQEGAFGVRL KPDDDIIELF KNGRSSVSLG YIGIHELNIL VGRDIGREIL 

       430        440        450        460        470        480 
TKMNAHLKQW TERTGFAFSL YSTPAENLCY RFCKLDTEKY GSVKDVTDKG WYTNSFHVSV 

       490        500        510        520        530        540 
EENITPFEKI SREAPYHFIA TGGHISYVEL PDMKNNLKGL EAVWDYAAQH LDYFGVNMPV 

       550        560        570        580        590        600 
DKCFTCGSTH EMTPTENGFV CSICGETDPK KMNTIRRTCG YLGNPNERGF NLGKNKEIMH 


RVKHQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb." Tomaschewski J., Rueger W. Nucleic Acids Res. 15:3632-3633(1987) [PubMed: 3575111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4." Barth K.A., Powell D., Trupin M., Mosig G. Genetics 120:329-343(1988) [PubMed: 2974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-181.
[4]	"The product of the split sunY gene of bacteriophage T4 is a processed protein." Zeh A., Shub D.A. J. Bacteriol. 173:6980-6985(1991) [PubMed: 1938898] [Abstract] Cited for: IDENTIFICATION.
[5]	"A possible glycine radical in anaerobic ribonucleotide reductase from Escherichia coli: nucleotide sequence of the cloned nrdD gene." Sun X., Harder J., Krook M., Joernvall H., Sjoeberg B.-M., Reichard P. Proc. Natl. Acad. Sci. U.S.A. 90:577-581(1993) [PubMed: 8421692] [Abstract] Cited for: POSSIBLE FUNCTION.
[6]	"Intron-containing T4 bacteriophage gene sunY encodes an anaerobic ribonucleotide reductase." Young P., Oehman M., Xu M.Q., Shub D.A., Sjoeberg B.-M. J. Biol. Chem. 269:20229-20232(1994) [PubMed: 8051113] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Bacteriophage T4 anaerobic ribonucleotide reductase contains a stable glycyl radical at position 580." Young P., Andersson J., Sahlin M., Sjoeberg B.-M. J. Biol. Chem. 271:20770-20775(1996) [PubMed: 8702830] [Abstract] Cited for: CHARACTERIZATION.
[8]	"A glycyl radical site in the crystal structure of a class III ribonucleotide reductase." Logan D.T., Andersson J., Sjoeberg B.-M., Nordlund P. Science 283:1499-1504(1999) [PubMed: 10066165] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS).
[9]	"A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase." Logan D.T., Mulliez E., Larsson K.-M., Bodevin S., Atta M., Garnaud P.E., Sjoeberg B.-M., Fontecave M. Proc. Natl. Acad. Sci. U.S.A. 100:3826-3831(2003) [PubMed: 12655046] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y00122 Genomic DNA. Translation: CAA68308.1.
Y00122 Genomic DNA. Translation: CAA68310.1. Sequence problems.
AF158101 Genomic DNA. Translation: AAD42633.1.
X12629 Genomic DNA. Translation: CAA31150.1.

PIR

Z6BPT9. E29284.
Z4BPT9. S01907.

RefSeq

NP_049690.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H78	X-ray	2.50	A	1-605	[»]
1H79	X-ray	2.90	A	1-605	[»]
1H7A	X-ray	2.75	A	1-605	[»]
1H7B	X-ray	2.45	A	1-605	[»]
1HK8	X-ray	2.45	A	1-605	[»]

ProteinModelPortal

P07071.

SMR

P07071. Positions 27-589.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258655.

GenomeReviews

Gene locus T4p077 in contig AF158101_GR.

Phylogenomic databases

ProtClustDB

CLSP2343308.

Family and domain databases

InterPro

IPR001150. Form_AcTrfase_GR.
IPR019777. Form_AcTrfase_GR_CS.
IPR012833. NrdD.
[Graphical view]

Pfam

PF01228. Gly_radical. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02487. NrdD. 1 hit.

PROSITE

PS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NRDD_BPT4

Accession

Primary (citable) accession number: P07071
Secondary accession number(s): P07073, Q38428, Q9T0V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2002
Last modified:	September 21, 2011
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents