Reviewed, UniProtKB/Swiss-Prot P07056 (UCRI_NEUCR)

Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Complex III subunit 5

Gene names

ORF Names:

NCU06606

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	Fungal bc1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins By similarity.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion
	Chain	33 – 231	199	Cytochrome b-c1 complex subunit Rieske, mitochondrial		PRO_0000030681
Regions
	Transmembrane	62 – 86	25	Potential
	Domain	134 – 229	96	Rieske
	Region	33 – 61	29	Hydrophilic
	Region	87 – 231	145	Hydrophilic
Sites
	Metal binding	174	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	176	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	193	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	196	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	179 ↔ 195		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P07056-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 66B780FE78227351	FASTA	231	24,771
	10 20 30 40 50 60 MAPVSIVSRA AMRAAAAPAR AVRALTTSTA LQGSSSSTFE SPFKGESKAA KVPDFGKYMS 70 80 90 100 110 120 KAPPSTNMLF SYFMVGTMGA ITAAGAKSTI QEFLKNMSAS ADVLAMAKVE VDLNAIPEGK 130 140 150 160 170 180 NVIIKWRGKP VFIRHRTPAE IEEANKVNVA TLRDPETDAD RVKKPEWLVM LGVCTHLGCV 190 200 210 220 230 PIGEAGDYGG WFCPCHGSHY DISGRIRKGP APLNLEIPLY EFPEEGKLVI G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing." Harnisch U., Weiss H., Sebald W. Eur. J. Biochem. 149:95-99(1985) [PubMed: 2986972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02472 Genomic DNA. Translation: CAA26308.1. AABX02000028 Genomic DNA. Translation: EAA33112.1.
PIR	RDNCUF. A24612.
RefSeq	XP_962348.1.
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
SMR	P07056. Positions 70-231.
ModBase	Search...
Protein-protein interaction databases
STRING	P07056.
Genome annotation databases
GeneID	3878496.
KEGG	ncr:NCU06606.
NMPDR	fig|5141.1.peg.4394.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-001465-MON.
BRENDA	1.10.2.2. 266.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_NEUCR

Accession

Primary (citable) accession number: P07056
Secondary accession number(s): Q7S9Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	September 1, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)