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Protein

Repressor c protein

Gene

repc

Organism
Escherichia phage D108 (Bacteriophage D108)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes latency by binding operators O1 and O2 in the enhancer/operator region, thereby repressing the transcription from the Pe (early) promoter and blocking the expression of the genes required for replication (lytic growth). Competes with DDE-recombinase A for binding to the internal activation sequence (IAS), which overlaps O1 and O2. The outcome of this competition determines if the virus enters latency or starts replication. Makes the cell immune to superinfection by repressing genes expression of any subsequent incoming viral genome (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processHost-virus interaction, Latency-replication decision, Transcription, Transcription regulation, Viral latency

Names & Taxonomyi

Protein namesi
Recommended name:
Repressor c protein
Short name:
Repc
Alternative name(s):
CI
Gene namesi
Name:repc
Synonyms:c
OrganismiEscherichia phage D108 (Bacteriophage D108)
Taxonomic identifieri665033 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMuvirusunclassified Mu-like viruses
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000320 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000775881 – 174Repressor c proteinAdd BLAST174

Expressioni

Inductioni

Expressed in the early phase of the viral replicative cycle. When present at high concentration, negatively regulates its own expression by binding to O3 (PcM promoter). PcM promoter, and thus Repc expression, is blocked by Ner. The host SsrA, the ClpXP host protease that degrades Repc, the Lon protease, and the stationary phase-specific sigma factor RpoS are all influencing viral repression in response to either temperature or stationary growth phase. Decreased availability of host SsrA in growing cells would favor latency, whereas starvation would favor Repc degradation and hence induction.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer. Three homodimers assemble as a homohexamer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP07040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 73HTH Mu-typePROSITE-ProRule annotationAdd BLAST68

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 174Recognition by host ClpX-ClpPBy similarity5

Domaini

The winged HTH N-terminal domain cooperatively binds to the enhancer/operator region of the Pe promoter. The C-terminal domain (CTD) regulates DNA binding by the N-terminal domain and degradation by ClpX-ClpP protease.

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090002BS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR009061. DNA-bd_dom_put.
IPR003314. Mu-type_HTH.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF02316. HTH_Tnp_Mu_1. 1 hit.
SUPFAMiSSF46955. SSF46955. 1 hit.
PROSITEiView protein in PROSITE
PS51702. HTH_MU. 1 hit.

Sequencei

Sequence statusi: Complete.

P07040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSFEIKEWF NAKELEGMPG VPKLATNITR KAVAEDWVKR QRHGGKGVAY
60 70 80 90 100
EYHINSLPEE TRRAIKGASL SDKPVHTSIV HTVDERLIYA MSFLTPDEQA
110 120 130 140 150
AAVEIIRVAG IKGLMPTIVS KDKALEALGI TVEQQKTLQT LQALPPEKVR
160 170
EILSQYEGKE HNFPVRENDV KKAV
Length:174
Mass (Da):19,518
Last modified:April 1, 1988 - v1
Checksum:i2DA5D6CCDCB4C585
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50Y → N in ACV50260 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03847 Genomic DNA. Translation: CAA27474.1.
GQ357916 Genomic DNA. Translation: ACV50260.1.
PIRiS07370.
RefSeqiYP_003335749.1. NC_013594.1.

Genome annotation databases

GeneIDi8658869.
KEGGivg:8658869.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiREPC_BPD10
AccessioniPrimary (citable) accession number: P07040
Secondary accession number(s): C9DGK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 10, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families