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P07038 (PMA1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasma membrane ATPase
EC=3.6.3.6
Alternative name(s):
Proton pump
Gene names
Name:pma-1
ORF Names:B1D1.210, NCU01680
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 920920Plasma membrane ATPase
PRO_0000046268

Regions

Topological domain1 – 115115Cytoplasmic Probable
Transmembrane116 – 13823Helical; Name=1; Probable
Topological domain139 – 1402Extracellular Probable
Transmembrane141 – 16020Helical; Name=2; Probable
Topological domain161 – 291131Cytoplasmic Probable
Transmembrane292 – 31423Helical; Name=3; Probable
Topological domain315 – 3217Extracellular Probable
Transmembrane322 – 35433Helical; Name=4; Probable
Topological domain355 – 687333Cytoplasmic Probable
Transmembrane688 – 71326Helical; Name=5; Probable
Topological domain714 – 7207Extracellular Probable
Transmembrane721 – 73818Helical; Name=6; Probable
Topological domain739 – 75416Cytoplasmic Probable
Transmembrane755 – 77925Helical; Name=7; Probable
Topological domain780 – 80627Extracellular Probable
Transmembrane807 – 82620Helical; Name=8; Probable
Transmembrane827 – 84721Helical; Name=9; Probable
Topological domain848 – 8536Extracellular Probable
Transmembrane854 – 87825Helical; Name=10; Probable
Topological domain879 – 92042Cytoplasmic Probable

Sites

Active site37814-aspartylphosphate intermediate By similarity
Metal binding6341Magnesium By similarity
Metal binding6381Magnesium By similarity

Experimental info

Sequence conflict81G → A in AAA33563. Ref.2
Sequence conflict8011I → M in AAA33563. Ref.2

Secondary structure

1 ............................................................................................................................................ 920
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07038 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 2A8F035F26337CF7

FASTA92099,887
        10         20         30         40         50         60 
MADHSASGAP ALSTNIESGK FDEKAAEAAA YQPKPKVEDD EDEDIDALIE DLESHDGHDA 

        70         80         90        100        110        120 
EEEEEEATPG GGRVVPEDML QTDTRVGLTS EEVVQRRRKY GLNQMKEEKE NHFLKFLGFF 

       130        140        150        160        170        180 
VGPIQFVMEG AAVLAAGLED WVDFGVICGL LLLNAVVGFV QEFQAGSIVD ELKKTLALKA 

       190        200        210        220        230        240 
VVLRDGTLKE IEAPEVVPGD ILQVEEGTII PADGRIVTDD AFLQVDQSAL TGESLAVDKH 

       250        260        270        280        290        300 
KGDQVFASSA VKRGEAFVVI TATGDNTFVG RAAALVNAAS GGSGHFTEVL NGIGTILLIL 

       310        320        330        340        350        360 
VIFTLLIVWV SSFYRSNPIV QILEFTLAIT IIGVPVGLPA VVTTTMAVGA AYLAKKKAIV 

       370        380        390        400        410        420 
QKLSAIESLA GVEILCSDKT GTLTKNKLSL HDPYTVAGVD PEDLMLTACL AASRKKKGID 

       430        440        450        460        470        480 
AIDKAFLKSL KYYPRAKSVL SKYKVLQFHP FDPVSKKVVA VVESPQGERI TCVKGAPLFV 

       490        500        510        520        530        540 
LKTVEEDHPI PEEVDQAYKN KVAEFATRGF RSLGVARKRG EGSWEILGIM PCMDPPRHDT 

       550        560        570        580        590        600 
YKTVCEAKTL GLSIKMLTGD AVGIARETSR QLGLGTNIYN AERLGLGGGG DMPGSEVYDF 

       610        620        630        640        650        660 
VEAADGFAEV FPQHKYNVVE ILQQRGYLVA MTGDGVNDAP SLKKADTGIA VEGSSDAARS 

       670        680        690        700        710        720 
AADIVFLAPG LGAIIDALKT SRQIFHRMYA YVVYRIALSI HLEIFLGLWI AILNRSLNIE 

       730        740        750        760        770        780 
LVVFIAIFAD VATLAIAYDN APYSQTPVKW NLPKLWGMSV LLGVVLAVGT WITVTTMYAQ 

       790        800        810        820        830        840 
GENGGIVQNF GNMDEVLFLQ ISLTENWLIF ITRANGPFWS SIPSWQLSGA IFLVDILATC 

       850        860        870        880        890        900 
FTIWGWFEHS DTSIVAVVRI WIFSFGIFCI MGGVYYILQD SVGFDNLMHG KSPKGNQKQR 

       910        920 
SLEDFVVSLQ RVSTQHEKSQ

« Hide

References

« Hide 'large scale' references
[1]"Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences."
Hager K.M., Mandala S.M., Davenport J.W., Speicher D.W., Benz E.J. Jr., Slayman C.W.
Proc. Natl. Acad. Sci. U.S.A. 83:7693-7697(1986) [PubMed: 2876429] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase."
Addison R.
J. Biol. Chem. 261:14896-14901(1986) [PubMed: 2876992] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed: 12712197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[5]"Cysteine 532 and cysteine 545 are the N-ethylmaleimide-reactive residues of the Neurospora plasma membrane H+-ATPase."
Pardo J.P., Slayman C.W.
J. Biol. Chem. 264:9373-9379(1989) [PubMed: 2524486] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
[6]"Identification of the major cytoplasmic regions of the Neurospora crassa plasma membrane H(+)-ATPase using protein chemical techniques."
Scarborough G.A., Hennessey J.P. Jr.
J. Biol. Chem. 265:16145-16149(1990) [PubMed: 2144525] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, TOPOLOGY.
[7]"Identification of the membrane-embedded regions of the Neurospora crassa plasma membrane H(+)-ATPase."
Rao U.S., Hennessey J.P. Jr., Scarborough G.A.
J. Biol. Chem. 266:14740-14746(1991) [PubMed: 1830591] [Abstract]
Cited for: TOPOLOGY.
[8]"Cytoplasmic location of amino acids 359-440 of the Neurospora crassa plasma membrane H(+)-ATPase."
Rao U.S., Bauzon D.D., Scarborough G.A.
Biochim. Biophys. Acta 1108:153-158(1992) [PubMed: 1386255] [Abstract]
Cited for: TOPOLOGY.
[9]"Topology of the Neurospora plasma membrane H(+)-ATPase. Localization of a transmembrane segment."
Lin A., Addison R.
J. Biol. Chem. 269:3887-3890(1994) [PubMed: 8106434] [Abstract]
Cited for: TOPOLOGY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M14085 Genomic DNA. Translation: AAA33561.1.
J02602 Genomic DNA. Translation: AAA33563.1.
AL355927 Genomic DNA. Translation: CAB91270.1.
AABX02000005 Genomic DNA. Translation: EAA27650.1.
PIRPXNCP. A26497.
RefSeqXP_956886.1. XM_951793.2.
UniGeneNcr.15464.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MHSelectron microscopy8.00A/B1-920[»]
ProteinModelPortalP07038.
SMRP07038. Positions 1-920.
ModBaseSearch...

Protein-protein interaction databases

STRINGP07038.

Protein family/group databases

TCDB3.A.3.3.1. P-type ATPase (P-ATPase) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000001834; EFNCRP00000001834; EFNCRG00000001832.
GeneID3873048.
KEGGncr:NCU01680.
NMPDRfig|5141.1.peg.6052.

Phylogenomic databases

eggNOGfuNOG06156.
GeneTreeEFGT00050000000742.
OrthoDBEOG4M68RQ.

Enzyme and pathway databases

BioCycNCRA-XX3-01:NCRA-XX3-01-005390-MONOMER.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 2 hits.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits.
KOK01535.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA1_NEUCR
AccessionPrimary (citable) accession number: P07038
Secondary accession number(s): Q7RV59
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: December 14, 2011
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents