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Protein

Regulatory protein SdiA

Gene

sdiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Activates cell division by specifically increasing transcription from one of the two promoters that lie immediately upstream of the ftsQAZ gene cluster. Activates ydiV expression in response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer oxoC6).2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi197 – 21620H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of cytokinesis Source: EcoCyc
  • positive regulation of DNA-templated transcription, initiation Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Cell division, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD02198.
ECOL316407:JW1901-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein SdiA
Gene namesi
Name:sdiA
Ordered Locus Names:b1916, JW1901
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10935. sdiA.

Pathology & Biotechi

Disruption phenotypei

A double sdiA/ydiV deletion mutant leads to decreased cAMP levels which inhibits quorum sensing system 2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Regulatory protein SdiAPRO_0000184187Add
BLAST

Proteomic databases

PaxDbiP07026.
PRIDEiP07026.

Expressioni

Inductioni

Repressed by glucose.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259602. 108 interactions.
IntActiP07026. 8 interactions.
STRINGi511145.b1916.

Structurei

Secondary structure

1
240
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1914Combined sources
Helixi23 – 3614Combined sources
Beta strandi40 – 478Combined sources
Beta strandi51 – 544Combined sources
Beta strandi57 – 615Combined sources
Helixi65 – 739Combined sources
Helixi76 – 783Combined sources
Helixi81 – 833Combined sources
Helixi85 – 873Combined sources
Beta strandi92 – 943Combined sources
Helixi97 – 1004Combined sources
Helixi104 – 1129Combined sources
Beta strandi117 – 1248Combined sources
Beta strandi130 – 1389Combined sources
Helixi147 – 16721Combined sources
Turni171 – 1733Combined sources
Helixi182 – 19211Combined sources
Helixi197 – 2048Combined sources
Helixi208 – 22215Combined sources
Helixi227 – 23610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AVXNMR-A1-171[»]
4LFUX-ray2.26A1-240[»]
4LGWX-ray2.70A1-240[»]
ProteinModelPortaliP07026.
SMRiP07026. Positions 5-240.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07026.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini173 – 23866HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Domaini

Binding to the autoinducer occurs via the N-terminal 170 residues; as E.coli does not produce LuxI autoinducers endogenously it should be able to bind to a number of different AI-1 autoinducers, which would enable it to detect other bacteria. This was shown to be the case.

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP07026.
KOiK07782.
OMAiAWMSHYQ.
PhylomeDBiP07026.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV
60 70 80 90 100
PFTRPKVAFY TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF
110 120 130 140 150
SEAQPLWEAA RAHGLRRGVT QYLMLPNRAL GFLSFSRCSA REIPILSDEL
160 170 180 190 200
QLKMQLLVRE SLMALMRLND EIVMTPEMNF SKREKEILRW TAEGKTSAEI
210 220 230 240
AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI
Length:240
Mass (Da):28,117
Last modified:November 1, 1997 - v2
Checksum:iC04CCC50C06135C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 1289TQYLMLPNR → HSVFNAAQTG in CAA27327 (PubMed:3515318).Curated
Sequence conflicti140 – 1401A → R in CAA27327 (PubMed:3515318).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03691 Genomic DNA. Translation: CAA27327.1.
U00096 Genomic DNA. Translation: AAC74983.1.
AP009048 Genomic DNA. Translation: BAA15736.1.
PIRiA64955. QQECU1.
RefSeqiNP_416426.1. NC_000913.3.
WP_001152715.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74983; AAC74983; b1916.
BAA15736; BAA15736; BAA15736.
GeneIDi946421.
KEGGiecj:JW1901.
eco:b1916.
PATRICi32119167. VBIEscCol129921_1998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03691 Genomic DNA. Translation: CAA27327.1.
U00096 Genomic DNA. Translation: AAC74983.1.
AP009048 Genomic DNA. Translation: BAA15736.1.
PIRiA64955. QQECU1.
RefSeqiNP_416426.1. NC_000913.3.
WP_001152715.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AVXNMR-A1-171[»]
4LFUX-ray2.26A1-240[»]
4LGWX-ray2.70A1-240[»]
ProteinModelPortaliP07026.
SMRiP07026. Positions 5-240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259602. 108 interactions.
IntActiP07026. 8 interactions.
STRINGi511145.b1916.

Proteomic databases

PaxDbiP07026.
PRIDEiP07026.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74983; AAC74983; b1916.
BAA15736; BAA15736; BAA15736.
GeneIDi946421.
KEGGiecj:JW1901.
eco:b1916.
PATRICi32119167. VBIEscCol129921_1998.

Organism-specific databases

EchoBASEiEB0928.
EcoGeneiEG10935. sdiA.

Phylogenomic databases

eggNOGiENOG4105VJY. Bacteria.
COG2771. LUCA.
HOGENOMiHOG000111053.
InParanoidiP07026.
KOiK07782.
OMAiAWMSHYQ.
PhylomeDBiP07026.

Enzyme and pathway databases

BioCyciEcoCyc:PD02198.
ECOL316407:JW1901-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP07026.
PROiP07026.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.30.450.80. 1 hit.
InterProiIPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF75516. SSF75516. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSDIA_ECOLI
AccessioniPrimary (citable) accession number: P07026
Secondary accession number(s): P76313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.