Regulatory protein SdiA - Escherichia coli (strain K12)

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P07026 (SDIA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulatory protein SdiA

Gene names

Name:	sdiA
Ordered Locus Names:	b1916, JW1901

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates cell division by specifically increasing transcription from one of the two promoters that lie immediately upstream of the ftsQAZ gene cluster. Activates ydiV expression in response to extracellular autoinducer AI-1 (Vibrio fischeri autoinducer oxoC6). Ref.5 Ref.6
Induction	Repressed by glucose. Ref.6
Domain	Binding to the autoinducer occurs via the N-terminal 170 residues; as E.coli does not produce LuxI autoinducers endogenously it should be able to bind to a number of different AI-1 autoinducers, which would enable it to detect other bacteria. This was shown to be the case.
Disruption phenotype	A double sdiA/ydiV deletion mutant leads to decreased cAMP levels which inhibits quorum sensing system 2. Ref.6
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Regulatory protein SdiA

PRO_0000184187

Regions

Domain

173 – 238

HTH luxR-type

DNA binding

197 – 216

H-T-H motif By similarity

Experimental info

Sequence conflict

120 – 128

TQYLMLPNR → HSVFNAAQTG in CAA27327. Ref.1

Sequence conflict

140

A → R in CAA27327. Ref.1

Secondary structure

240

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P07026 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C04CCC50C06135C4
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FASTA

240

28,117

        10         20         30         40         50         60 
MQDKDFFSWR RTMLLRFQRM ETAEEVYHEI ELQAQQLEYD YYSLCVRHPV PFTRPKVAFY 

        70         80         90        100        110        120 
TNYPEAWVSY YQAKNFLAID PVLNPENFSQ GHLMWNDDLF SEAQPLWEAA RAHGLRRGVT 

       130        140        150        160        170        180 
QYLMLPNRAL GFLSFSRCSA REIPILSDEL QLKMQLLVRE SLMALMRLND EIVMTPEMNF 

       190        200        210        220        230        240 
SKREKEILRW TAEGKTSAEI AMILSISENT VNFHQKNMQK KINAPNKTQV ACYAAATGLI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple control elements for the uvrC gene unit of Escherichia coli." Sharma S., Stark T.F., Beattie W.G., Moses R.E. Nucleic Acids Res. 14:2301-2318(1986) [PubMed: 3515318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A factor that positively regulates cell division by activating transcription of the major cluster of essential cell division genes of Escherichia coli." Wang X., de Boer P.A.J., Rothfield L.I. EMBO J. 10:3363-3372(1991) [PubMed: 1915297] [Abstract] Cited for: FUNCTION.
[6]	"An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli." Zhou X., Meng X., Sun B. Cell Res. 18:937-948(2008) [PubMed: 18560382] [Abstract] Cited for: ROLE IN YDIV REGULATION, REPRESSION BY GLUCOSE, DISRUPTION PHENOTYPE. Strain: K12 / W3110 / ZK126.
[7]	"Structure of the Escherichia coli quorum sensing protein SdiA: activation of the folding switch by acyl homoserine lactones." Yao Y., Martinez-Yamout M.A., Dickerson T.J., Brogan A.P., Wright P.E., Dyson H.J. J. Mol. Biol. 355:262-273(2006) [PubMed: 16307757] [Abstract] Cited for: STRUCTURE BY NMR OF 3-171 IN COMPLEX WITH AUTOINDUCER, BINDING OF HOMOSERINE LACTONE DERIVATIVES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03691 Genomic DNA. Translation: CAA27327.1.
U00096 Genomic DNA. Translation: AAC74983.1.
AP009048 Genomic DNA. Translation: BAA15736.1.

PIR

QQECU1. A64955.

RefSeq

NP_416426.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AVX	NMR	-	A	3-171	[»]

ProteinModelPortal

P07026.

SMR

P07026. Positions 1-240.

ModBase

Search...

Protein-protein interaction databases

IntAct

P07026. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000450; EBESCP00000000450; EBESCG00000000372.
EBESCT00000014436; EBESCP00000013727; EBESCG00000013497.

GeneID

946421.

GenomeReviews

Gene locus JW1901 in contig AP009048_GR.
Gene locus b1916 in contig U00096_GR.

KEGG

ecj:JW1901.
eco:b1916.

PATRIC

32119167. VBIEscCol129921_1998.

Organism-specific databases

EchoBASE

EB0928.

EcoGene

EG10935. sdiA.

Phylogenomic databases

eggNOG

COG2771.

GeneTree

EBGT00050000010572.

HOGENOM

HBG577588.

OMA

PAVAIFD.

PhylomeDB

P07026.

ProtClustDB

PRK10188.

Enzyme and pathway databases

BioCyc

EcoCyc:PD02198.

Gene expression databases

Genevestigator

P07026.

Family and domain databases

InterPro

IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR005143. TF_LuxR_autoind-bd_dom.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.450.80. TF_LuxR_autoind-bd_dom. 1 hit.
G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

K07782.

Pfam

PF03472. Autoind_bind. 1 hit.
PF00196. GerE. 1 hit.
[Graphical view]

PRINTS

PR00038. HTHLUXR.

SMART

SM00421. HTH_LUXR. 1 hit.
[Graphical view]

SUPFAM

SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
SSF75516. TF_LuxR_autoind-bd_dom. 1 hit.

PROSITE

PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SDIA_ECOLI

Accession

Primary (citable) accession number: P07026
Secondary accession number(s): P76313

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents