Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein UshA

Gene

ushA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.

Catalytic activityi

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Enzyme regulationi

The activity of this protein is inhibited by an intracellular protein inhibitor.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Zinc 11
Metal bindingi43Zinc 11
Metal bindingi84Zinc 11
Metal bindingi84Zinc 21
Metal bindingi116Zinc 21
Sitei117Transition state stabilizer1
Sitei120Transition state stabilizer1
Metal bindingi217Zinc 21
Metal bindingi252Zinc 21
Metal bindingi254Zinc 11

GO - Molecular functioni

  • 5'-nucleotidase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW
  • UDP-sugar diphosphatase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:USHA-MONOMER.
ECOL316407:JW0469-MONOMER.
MetaCyc:USHA-MONOMER.
BRENDAi3.1.3.5. 2026.
SABIO-RKP07024.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UshA
Including the following 2 domains:
UDP-sugar hydrolase (EC:3.6.1.45)
Alternative name(s):
UDP-sugar diphosphatase
UDP-sugar pyrophosphatase
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Gene namesi
Name:ushA
Ordered Locus Names:b0480, JW0469
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11060. ushA.

Subcellular locationi

  • Periplasm

  • Note: Exported from the cell, except a small proportion that is internally localized.

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 252 PublicationsAdd BLAST25
ChainiPRO_000000003126 – 550Protein UshAAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi258 ↔ 275

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP07024.
PaxDbiP07024.
PRIDEiP07024.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi4259843. 4 interactors.
DIPiDIP-11096N.
IntActiP07024. 10 interactors.
MINTiMINT-1256646.
STRINGi511145.b0480.

Structurei

Secondary structure

1550
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 39Combined sources8
Helixi56 – 73Combined sources18
Beta strandi76 – 81Combined sources6
Beta strandi86 – 88Combined sources3
Helixi90 – 93Combined sources4
Turni94 – 97Combined sources4
Helixi98 – 107Combined sources10
Beta strandi111 – 113Combined sources3
Helixi116 – 119Combined sources4
Helixi123 – 132Combined sources10
Beta strandi140 – 144Combined sources5
Turni145 – 147Combined sources3
Beta strandi150 – 160Combined sources11
Beta strandi163 – 171Combined sources9
Turni173 – 177Combined sources5
Beta strandi178 – 180Combined sources3
Helixi181 – 184Combined sources4
Beta strandi187 – 189Combined sources3
Helixi192 – 206Combined sources15
Beta strandi210 – 218Combined sources9
Helixi222 – 224Combined sources3
Helixi233 – 239Combined sources7
Beta strandi244 – 249Combined sources6
Beta strandi259 – 261Combined sources3
Beta strandi278 – 280Combined sources3
Beta strandi283 – 286Combined sources4
Beta strandi293 – 303Combined sources11
Beta strandi306 – 318Combined sources13
Beta strandi320 – 324Combined sources5
Beta strandi326 – 328Combined sources3
Beta strandi333 – 336Combined sources4
Helixi343 – 359Combined sources17
Beta strandi362 – 369Combined sources8
Helixi375 – 378Combined sources4
Helixi384 – 397Combined sources14
Beta strandi400 – 405Combined sources6
Helixi406 – 408Combined sources3
Beta strandi415 – 419Combined sources5
Helixi420 – 426Combined sources7
Beta strandi432 – 439Combined sources8
Helixi440 – 450Combined sources11
Beta strandi455 – 457Combined sources3
Beta strandi461 – 471Combined sources11
Beta strandi474 – 480Combined sources7
Beta strandi489 – 496Combined sources8
Helixi497 – 500Combined sources4
Helixi503 – 505Combined sources3
Beta strandi512 – 514Combined sources3
Beta strandi515 – 521Combined sources7
Helixi522 – 533Combined sources12
Helixi538 – 541Combined sources4
Beta strandi545 – 550Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HO5X-ray2.10A/B26-550[»]
1HP1X-ray1.70A26-541[»]
1HPUX-ray1.85A/B/C/D26-550[»]
1OI8X-ray2.10A/B26-550[»]
1OIDX-ray2.10A/B26-550[»]
1OIEX-ray2.33A26-550[»]
1USHX-ray1.73A1-550[»]
2USHX-ray2.22A/B1-550[»]
4WWLX-ray2.23A26-550[»]
ProteinModelPortaliP07024.
SMRiP07024.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07024.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 379Substrate binding5
Regioni498 – 504Substrate binding7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CGH. Bacteria.
COG0737. LUCA.
HOGENOMiHOG000247216.
InParanoidiP07024.
KOiK11751.
OMAiTLNILHI.
PhylomeDBiP07024.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLQRGVAL ALLTTFTLAS ETALAYEQDK TYKITVLHTN DHHGHFWRNE
60 70 80 90 100
YGEYGLAAQK TLVDGIRKEV AAEGGSVLLL SGGDINTGVP ESDLQDAEPD
110 120 130 140 150
FRGMNLVGYD AMAIGNHEFD NPLTVLRQQE KWAKFPLLSA NIYQKSTGER
160 170 180 190 200
LFKPWALFKR QDLKIAVIGL TTDDTAKIGN PEYFTDIEFR KPADEAKLVI
210 220 230 240 250
QELQQTEKPD IIIAATHMGH YDNGEHGSNA PGDVEMARAL PAGSLAMIVG
260 270 280 290 300
GHSQDPVCMA AENKKQVDYV PGTPCKPDQQ NGIWIVQAHE WGKYVGRADF
310 320 330 340 350
EFRNGEMKMV NYQLIPVNLK KKVTWEDGKS ERVLYTPEIA ENQQMISLLS
360 370 380 390 400
PFQNKGKAQL EVKIGETNGR LEGDRDKVRF VQTNMGRLIL AAQMDRTGAD
410 420 430 440 450
FAVMSGGGIR DSIEAGDISY KNVLKVQPFG NVVVYADMTG KEVIDYLTAV
460 470 480 490 500
AQMKPDSGAY PQFANVSFVA KDGKLNDLKI KGEPVDPAKT YRMATLNFNA
510 520 530 540 550
TGGDGYPRLD NKPGYVNTGF IDAEVLKAYI QKSSPLDVSV YEPKGEVSWQ
Length:550
Mass (Da):60,824
Last modified:November 1, 1997 - v2
Checksum:iDBA07B1C40C6C075
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti256P → T in CAA27532 (PubMed:3012467).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03895 Genomic DNA. Translation: CAA27532.1.
U82664 Genomic DNA. Translation: AAB40234.1.
U00096 Genomic DNA. Translation: AAC73582.1.
AP009048 Genomic DNA. Translation: BAE76259.1.
D73370 Genomic DNA. No translation available.
PIRiG64778. YXECUG.
RefSeqiNP_415013.1. NC_000913.3.
WP_000771748.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73582; AAC73582; b0480.
BAE76259; BAE76259; BAE76259.
GeneIDi947331.
KEGGiecj:JW0469.
eco:b0480.
PATRICi32116117. VBIEscCol129921_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03895 Genomic DNA. Translation: CAA27532.1.
U82664 Genomic DNA. Translation: AAB40234.1.
U00096 Genomic DNA. Translation: AAC73582.1.
AP009048 Genomic DNA. Translation: BAE76259.1.
D73370 Genomic DNA. No translation available.
PIRiG64778. YXECUG.
RefSeqiNP_415013.1. NC_000913.3.
WP_000771748.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HO5X-ray2.10A/B26-550[»]
1HP1X-ray1.70A26-541[»]
1HPUX-ray1.85A/B/C/D26-550[»]
1OI8X-ray2.10A/B26-550[»]
1OIDX-ray2.10A/B26-550[»]
1OIEX-ray2.33A26-550[»]
1USHX-ray1.73A1-550[»]
2USHX-ray2.22A/B1-550[»]
4WWLX-ray2.23A26-550[»]
ProteinModelPortaliP07024.
SMRiP07024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259843. 4 interactors.
DIPiDIP-11096N.
IntActiP07024. 10 interactors.
MINTiMINT-1256646.
STRINGi511145.b0480.

Proteomic databases

EPDiP07024.
PaxDbiP07024.
PRIDEiP07024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73582; AAC73582; b0480.
BAE76259; BAE76259; BAE76259.
GeneIDi947331.
KEGGiecj:JW0469.
eco:b0480.
PATRICi32116117. VBIEscCol129921_0500.

Organism-specific databases

EchoBASEiEB1053.
EcoGeneiEG11060. ushA.

Phylogenomic databases

eggNOGiENOG4105CGH. Bacteria.
COG0737. LUCA.
HOGENOMiHOG000247216.
InParanoidiP07024.
KOiK11751.
OMAiTLNILHI.
PhylomeDBiP07024.

Enzyme and pathway databases

BioCyciEcoCyc:USHA-MONOMER.
ECOL316407:JW0469-MONOMER.
MetaCyc:USHA-MONOMER.
BRENDAi3.1.3.5. 2026.
SABIO-RKP07024.

Miscellaneous databases

EvolutionaryTraceiP07024.
PROiP07024.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSHA_ECOLI
AccessioniPrimary (citable) accession number: P07024
Secondary accession number(s): P78274, Q2MBU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 172 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.