Reviewed, UniProtKB/Swiss-Prot P07023 (TYRA_ECOLI)

Names and origin

Protein names

Recommended name:
    T-protein
Including the following 2 domains:
    1- Recommended name:
            Chorismate mutase
                Short name=CM
              EC=5.4.99.5
    2- Recommended name:
            Prephenate dehydrogenase
                Short name=PDH
              EC=1.3.1.12

Gene names

Name:	tyrA
Ordered Locus Names:	b2600, JW2581

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Chorismate = prephenate. Prephenate + NAD+ = 4-hydroxyphenylpyruvate + CO2 + NADH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; L-tyrosine from prephenate: step 1/2. Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 chorismate mutase domain. Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro chorismate mutase activity Inferred from electronic annotation. Source: EC prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	T-protein		PRO_0000119195
Regions
	Domain	1 – 90	90	Chorismate mutase
	Domain	99 – 361	263	Prephenate/arogenate dehydrogenase

Sequences

	Sequence		Length	Mass (Da)
	P07023-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 481D0A03DE2AC832	FASTA	373	42,043
	10 20 30 40 50 60 MVAELTALRD QIDEVDKALL NLLAKRLELV AEVGEVKSRF GLPIYVPERE ASMLASRRAE 70 80 90 100 110 120 AEALGVPPDL IEDVLRRVMR ESYSSENDKG FKTLCPSLRP VVIVGGGGQM GRLFEKMLTL 130 140 150 160 170 180 SGYQVRILEQ HDWDRAADIV ADAGMVIVSV PIHVTEQVIG KLPPLPKDCI LVDLASVKNG 190 200 210 220 230 240 PLQAMLVAHD GPVLGLHPMF GPDSGSLAKQ VVVWCDGRKP EAYQWFLEQI QVWGARLHRI 250 260 270 280 290 300 SAVEHDQNMA FIQALRHFAT FAYGLHLAEE NVQLEQLLAL SSPIYRLELA MVGRLFAQDP 310 320 330 340 350 360 QLYADIIMSS ERNLALIKRY YKRFGEAIEL LEQGDKQAFI DSFRKVEHWF GDYAQRFQSE 370 SRVLLRQAND NRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12." Hudson G.S., Davidson B.E. J. Mol. Biol. 180:1023-1051(1984) [PubMed: 6396419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M10431 Genomic DNA. Translation: AAA24331.1. U00096 Genomic DNA. Translation: AAC75649.1. AP009048 Genomic DNA. Translation: BAA16485.2.
PIR	KMECTD. A30274.
RefSeq	AP_003181.1. NP_417091.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:11059N.
2-D gel databases
ECO2DBASE	F037.9. 6TH EDITION.
Genome annotation databases
GeneID	947115.
GenomeReviews	Gene locus JW2581 in contig AP009048_GR. Gene locus b2600 in contig U00096_GR.
KEGG	ecj:JW2581. eco:b2600.
Organism-specific databases
EchoBASE	EB1032.
EcoGene	EG11039. tyrA.
CMR	Search...
Phylogenomic databases
HOGENOM	P07023.
OMA	P07023. RTMRESY.
Enzyme and pathway databases
BioCyc	EcoCyc:CHORISMUTPREPHENDEHYDROG-MON. MetaCyc:CHORISMUTPREPHENDEHYDROG-MON.
Family and domain databases
InterPro	IPR008244. Chor_mut/prephenate_DH_T. IPR002701. Chorismate_mut. IPR011277. CM_T. IPR003099. Prephen_DH. [Graphical view]
PANTHER	PTHR21363. Prephen_DH. 1 hit.
Pfam	PF01817. CM_2. 1 hit. PF02153. PDH. 1 hit. [Graphical view]
PIRSF	PIRSF001499. Chor_mut_pdh_Tpr. 1 hit.
TIGRFAMs	TIGR01799. CM_T. 1 hit.
PROSITE	PS51168. CHORISMATE_MUT_2. 1 hit. PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRA_ECOLI

Accession

Primary (citable) accession number: P07023
Secondary accession number(s): P78205, P78206

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)