T-protein - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P07023 (TYRA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
T-protein

Including the following 2 domains:

Chorismate mutase
Short name=CM
EC=5.4.99.5
Prephenate dehydrogenase
Short name=PDH
EC=1.3.1.12

Gene names

Name:	tyrA
Ordered Locus Names:	b2600, JW2581

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Chorismate = prephenate. Prephenate + NAD⁺ = 4-hydroxyphenylpyruvate + CO₂ + NADH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NAD(+) route): step 1/1. Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 chorismate mutase domain. Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	L-phenylalanine biosynthetic process Inferred from direct assay. Source: EcoCyc tyrosine biosynthetic process Inferred from direct assay. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chorismate mutase activity Inferred from direct assay. Source: EcoCyc nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase activity Inferred from direct assay. Source: EcoCyc protein homodimerization activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 373

373

T-protein

PRO_0000119195

Regions

Domain

1 – 90

Chorismate mutase

Domain

99 – 361

263

Prephenate/arogenate dehydrogenase

Sequences

Sequence

Length

Mass (Da)

Tools

P07023 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 481D0A03DE2AC832
Show »

FASTA

373

42,043

        10         20         30         40         50         60 
MVAELTALRD QIDEVDKALL NLLAKRLELV AEVGEVKSRF GLPIYVPERE ASMLASRRAE 

        70         80         90        100        110        120 
AEALGVPPDL IEDVLRRVMR ESYSSENDKG FKTLCPSLRP VVIVGGGGQM GRLFEKMLTL 

       130        140        150        160        170        180 
SGYQVRILEQ HDWDRAADIV ADAGMVIVSV PIHVTEQVIG KLPPLPKDCI LVDLASVKNG 

       190        200        210        220        230        240 
PLQAMLVAHD GPVLGLHPMF GPDSGSLAKQ VVVWCDGRKP EAYQWFLEQI QVWGARLHRI 

       250        260        270        280        290        300 
SAVEHDQNMA FIQALRHFAT FAYGLHLAEE NVQLEQLLAL SSPIYRLELA MVGRLFAQDP 

       310        320        330        340        350        360 
QLYADIIMSS ERNLALIKRY YKRFGEAIEL LEQGDKQAFI DSFRKVEHWF GDYAQRFQSE 

       370 
SRVLLRQAND NRQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12." Hudson G.S., Davidson B.E. J. Mol. Biol. 180:1023-1051(1984) [PubMed: 6396419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M10431 Genomic DNA. Translation: AAA24331.1. U00096 Genomic DNA. Translation: AAC75649.1. AP009048 Genomic DNA. Translation: BAA16485.2.
PIR	KMECTD. A30274.
RefSeq	NP_417091.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P07023.
SMR	P07023. Positions 3-367.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11059N.
IntAct	P07023. 1 interaction.
MINT	MINT-1255670.
2D gel databases
ECO2DBASE	F037.9. 6TH EDITION.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003551; EBESCP00000003551; EBESCG00000002901. EBESCT00000017395; EBESCP00000016686; EBESCG00000016451.
GeneID	947115.
GenomeReviews	Gene locus JW2581 in contig AP009048_GR. Gene locus b2600 in contig U00096_GR.
KEGG	ecj:JW2581. eco:b2600.
PATRIC	32120599. VBIEscCol129921_2698.
Organism-specific databases
EchoBASE	EB1032.
EcoGene	EG11039. tyrA.
Phylogenomic databases
eggNOG	COG0287.
GeneTree	EBGT00050000010854.
HOGENOM	HBG538602.
OMA	HGLPIYA.
PhylomeDB	P07023.
ProtClustDB	PRK11199.
Enzyme and pathway databases
BioCyc	EcoCyc:CHORISMUTPREPHENDEHYDROG-MONOMER. MetaCyc:CHORISMUTPREPHENDEHYDROG-MONOMER.
Gene expression databases
Genevestigator	P07023.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR008244. Chor_mut/prephenate_DH_T. IPR002701. Chorismate_mutase. IPR020822. Chorismate_mutase_type_II. IPR011277. CM_T. IPR016040. NAD(P)-bd_dom. IPR003099. Prephen_DH. [Graphical view]
Gene3D	G3DSA:1.20.59.10. Chorismate_mutase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K14187.
Pfam	PF01817. CM_2. 1 hit. PF02153. PDH. 1 hit. [Graphical view]
PIRSF	PIRSF001499. Chor_mut_pdh_Tpr. 1 hit.
SMART	SM00830. CM_2. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF48600. Chorismate_mut. 1 hit.
TIGRFAMs	TIGR01799. CM_T. 1 hit.
PROSITE	PS51168. CHORISMATE_MUT_2. 1 hit. PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRA_ECOLI

Accession

Primary (citable) accession number: P07023
Secondary accession number(s): P78205, P78206

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents