Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methyl-accepting chemotaxis protein IV

Gene

tap

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates taxis toward dipeptides via an interaction with the periplasmic dipeptide-binding protein.
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.

GO - Molecular functioni

  • signal transducer activity Source: EcoCyc
  • transmembrane signaling receptor activity Source: EcoCyc

GO - Biological processi

  • chemotaxis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

BioCyciEcoCyc:TAP-MONOMER.
ECOL316407:JW1874-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-accepting chemotaxis protein IV
Short name:
MCP-IV
Alternative name(s):
Dipeptide chemoreceptor protein
Gene namesi
Name:tap
Ordered Locus Names:b1885, JW1874
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10987. tap.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Found predominantly at cell poles.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 3327HelicalSequence analysisAdd
BLAST
Topological domaini34 – 188155PeriplasmicSequence analysisAdd
BLAST
Transmembranei189 – 20921HelicalSequence analysisAdd
BLAST
Topological domaini210 – 533324CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Methyl-accepting chemotaxis protein IVPRO_0000110541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei293 – 2931Glutamate methyl ester (Gln)By similarity
Modified residuei300 – 3001Glutamate methyl ester (Gln)By similarity
Modified residuei307 – 3071Glutamate methyl ester (Gln)By similarity
Modified residuei489 – 4891Glutamate methyl ester (Glu)By similarity

Keywords - PTMi

Methylation

Proteomic databases

EPDiP07018.
PaxDbiP07018.

Interactioni

Protein-protein interaction databases

BioGridi4262072. 163 interactions.
DIPiDIP-10955N.
IntActiP07018. 2 interactions.
MINTiMINT-1309514.
STRINGi511145.b1885.

Structurei

3D structure databases

ProteinModelPortaliP07018.
SMRiP07018. Positions 23-166, 214-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 26453HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini269 – 498230Methyl-accepting transducerPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 methyl-accepting transducer domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000148074.
InParanoidiP07018.
KOiK05877.
OMAiAKYDEAW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP07018.

Family and domain databases

Gene3Di1.20.120.30. 1 hit.
InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF47170. SSF47170. 1 hit.
PROSITEiPS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR
60 70 80 90 100
AALAQTRAVM LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL
110 120 130 140 150
FKSFMAMTAG NEHVRGLQKE TEKSFARWHN DLEHQATWLE SNQLSDFLTA
160 170 180 190 200
PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ SQRNYQISAL VFISMIIVAA
210 220 230 240 250
IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY GRNEITAIFA
260 270 280 290 300
SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ
310 320 330 340 350
TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI
360 370 380 390 400
ATSSQKIGDI ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR
410 420 430 440 450
NLASRSAQAA KEIKGLIEES VNRVQQGSKL VNNAAATMID IVSSVTRVND
460 470 480 490 500
IMGEIASASE EQQRGIEQVA QAVSQMDQVT QQNASLVEEA AVATEQLANQ
510 520 530
ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS
Length:533
Mass (Da):57,512
Last modified:November 1, 1997 - v2
Checksum:i632570BE1E45DA38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti335 – 3351A → G in AAA23567 (PubMed:6305515).Curated
Sequence conflicti503 – 5031H → R in AAA23567 (PubMed:6305515).Curated
Sequence conflicti527 – 5337QIAPVVS → TNCASGILK in AAA23567 (PubMed:6305515).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01705 Genomic DNA. Translation: AAA23567.1.
U00096 Genomic DNA. Translation: AAC74955.1.
AP009048 Genomic DNA. Translation: BAA15701.1.
PIRiE64951. QRECM2.
RefSeqiNP_416399.1. NC_000913.3.
WP_000483239.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74955; AAC74955; b1885.
BAA15701; BAA15701; BAA15701.
GeneIDi946397.
KEGGiecj:JW1874.
eco:b1885.
PATRICi32119097. VBIEscCol129921_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01705 Genomic DNA. Translation: AAA23567.1.
U00096 Genomic DNA. Translation: AAC74955.1.
AP009048 Genomic DNA. Translation: BAA15701.1.
PIRiE64951. QRECM2.
RefSeqiNP_416399.1. NC_000913.3.
WP_000483239.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07018.
SMRiP07018. Positions 23-166, 214-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262072. 163 interactions.
DIPiDIP-10955N.
IntActiP07018. 2 interactions.
MINTiMINT-1309514.
STRINGi511145.b1885.

Proteomic databases

EPDiP07018.
PaxDbiP07018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74955; AAC74955; b1885.
BAA15701; BAA15701; BAA15701.
GeneIDi946397.
KEGGiecj:JW1874.
eco:b1885.
PATRICi32119097. VBIEscCol129921_1966.

Organism-specific databases

EchoBASEiEB0980.
EcoGeneiEG10987. tap.

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000148074.
InParanoidiP07018.
KOiK05877.
OMAiAKYDEAW.
OrthoDBiEOG6G4VQG.
PhylomeDBiP07018.

Enzyme and pathway databases

BioCyciEcoCyc:TAP-MONOMER.
ECOL316407:JW1874-MONOMER.

Miscellaneous databases

PROiP07018.

Family and domain databases

Gene3Di1.20.120.30. 1 hit.
InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF47170. SSF47170. 1 hit.
PROSITEiPS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sensory transducers of E. coli are composed of discrete structural and functional domains."
    Krikos A., Mutoh N., Boyd A., Simon M.I.
    Cell 33:615-622(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Isolation and identification of new inner membrane-associated proteins that localize to cell poles in Escherichia coli."
    Li G., Young K.D.
    Mol. Microbiol. 84:276-295(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMCP4_ECOLI
AccessioniPrimary (citable) accession number: P07018
Secondary accession number(s): P76300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: March 16, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.