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P07018 (MCP4_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-accepting chemotaxis protein IV
Short name=MCP-IV
Alternative name(s):
Dipeptide chemoreceptor protein
Gene names
Name:tap
Ordered Locus Names:b1885, JW1874
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates taxis toward dipeptides via an interaction with the periplasmic dipeptide-binding protein.

Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Contains 1 HAMP domain.

Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionTransducer
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processchemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontransmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Methyl-accepting chemotaxis protein IV
PRO_0000110541

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 3327Helical; Potential
Topological domain34 – 188155Periplasmic Potential
Transmembrane189 – 20921Helical; Potential
Topological domain210 – 533324Cytoplasmic Potential
Domain212 – 26453HAMP
Domain269 – 498230Methyl-accepting transducer

Amino acid modifications

Modified residue2931Glutamate methyl ester (Gln)
Modified residue3001Glutamate methyl ester (Gln)
Modified residue3071Glutamate methyl ester (Gln)
Modified residue4891Glutamate methyl ester (Glu)

Experimental info

Sequence conflict3351A → G in AAA23567. Ref.1
Sequence conflict5031H → R in AAA23567. Ref.1
Sequence conflict527 – 5337QIAPVVS → TNCASGILK in AAA23567. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P07018 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 632570BE1E45DA38

FASTA53357,512
        10         20         30         40         50         60 
MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR AALAQTRAVM 

        70         80         90        100        110        120 
LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL FKSFMAMTAG NEHVRGLQKE 

       130        140        150        160        170        180 
TEKSFARWHN DLEHQATWLE SNQLSDFLTA PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ 

       190        200        210        220        230        240 
SQRNYQISAL VFISMIIVAA IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY 

       250        260        270        280        290        300 
GRNEITAIFA SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ 

       310        320        330        340        350        360 
TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI ATSSQKIGDI 

       370        380        390        400        410        420 
ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR NLASRSAQAA KEIKGLIEES 

       430        440        450        460        470        480 
VNRVQQGSKL VNNAAATMID IVSSVTRVND IMGEIASASE EQQRGIEQVA QAVSQMDQVT 

       490        500        510        520        530 
QQNASLVEEA AVATEQLANQ ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS

« Hide

References

« Hide 'large scale' references
[1]"Sensory transducers of E. coli are composed of discrete structural and functional domains."
Krikos A., Mutoh N., Boyd A., Simon M.I.
Cell 33:615-622(1983) [PubMed: 6305515] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J01705 Genomic DNA. Translation: AAA23567.1.
U00096 Genomic DNA. Translation: AAC74955.1.
AP009048 Genomic DNA. Translation: BAA15701.1.
PIRQRECM2. E64951.
RefSeqNP_416399.1. NC_000913.2.

3D structure databases

ProteinModelPortalP07018.
SMRP07018. Positions 23-181, 213-516.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10955N.
IntActP07018. 2 interactions.
MINTMINT-1309514.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000004965; EBESCP00000004965; EBESCG00000004053.
EBESCT00000014861; EBESCP00000014152; EBESCG00000013921.
GeneID946397.
GenomeReviewsGene locus JW1874 in contig AP009048_GR.
Gene locus b1885 in contig U00096_GR.
KEGGecj:JW1874.
eco:b1885.
PATRIC32119097. VBIEscCol129921_1966.

Organism-specific databases

EchoBASEEB0980.
EcoGeneEG10987. tap.

Phylogenomic databases

eggNOGCOG0840.
GeneTreeEBGT00050000009142.
HOGENOMHBG752668.
OMASHEMHIG.
PhylomeDBP07018.
ProtClustDBPRK09793.

Enzyme and pathway databases

BioCycEcoCyc:TAP-MONOMER.

Gene expression databases

GenevestigatorP07018.

Family and domain databases

InterProIPR004090. Chemotax_Me-accpt_rcpt.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR004089. Chemotax_Me-accpt_rcpt_sig.
IPR003660. HAMP_linker_domain.
[Graphical view]
Gene3DG3DSA:1.20.120.30. Chemotax_Me-accpt_rcpt_lig-bd. 1 hit.
KOK05877.
PfamPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
PRINTSPR00260. CHEMTRNSDUCR.
SMARTSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMSSF47170. Chemotax_Me-accpt_rcpt_lig-bd. 1 hit.
PROSITEPS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMCP4_ECOLI
AccessionPrimary (citable) accession number: P07018
Secondary accession number(s): P76300
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents