Reviewed, UniProtKB/Swiss-Prot P07018 (MCP4_ECOLI)

Names and origin

Protein names

Recommended name:
    Methyl-accepting chemotaxis protein IV
      Short name=MCP-IV
Alternative name(s):
    Dipeptide chemoreceptor protein

Gene names

Name:	tap
Ordered Locus Names:	b1885, JW1874

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates taxis toward dipeptides via an interaction with the periplasmic dipeptide-binding protein. Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase cheR and removed by the methylesterase cheB.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Contains 1 HAMP domain. Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transducer
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transmembrane receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Methyl-accepting chemotaxis protein IV		PRO_0000110541
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 33	27	Potential
	Topological domain	34 – 188	155	Periplasmic Potential
	Transmembrane	189 – 209	21	Potential
	Topological domain	210 – 533	324	Cytoplasmic Potential
	Domain	212 – 264	53	HAMP
	Domain	269 – 498	230	Methyl-accepting transducer
Amino acid modifications
	Modified residue	293	1	Glutamate methyl ester (Gln)
	Modified residue	300	1	Glutamate methyl ester (Gln)
	Modified residue	307	1	Glutamate methyl ester (Gln)
	Modified residue	489	1	Glutamate methyl ester (Glu)
Experimental info
	Sequence conflict	335	1	A → G in AAA23567. Ref.1
	Sequence conflict	503	1	H → R in AAA23567. Ref.1
	Sequence conflict	527 – 533	7	QIAPVVS → TNCASGILK in AAA23567. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P07018-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 632570BE1E45DA38	FASTA	533	57,512
	10 20 30 40 50 60 MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR AALAQTRAVM 70 80 90 100 110 120 LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL FKSFMAMTAG NEHVRGLQKE 130 140 150 160 170 180 TEKSFARWHN DLEHQATWLE SNQLSDFLTA PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ 190 200 210 220 230 240 SQRNYQISAL VFISMIIVAA IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY 250 260 270 280 290 300 GRNEITAIFA SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ 310 320 330 340 350 360 TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI ATSSQKIGDI 370 380 390 400 410 420 ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR NLASRSAQAA KEIKGLIEES 430 440 450 460 470 480 VNRVQQGSKL VNNAAATMID IVSSVTRVND IMGEIASASE EQQRGIEQVA QAVSQMDQVT 490 500 510 520 530 QQNASLVEEA AVATEQLANQ ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sensory transducers of E. coli are composed of discrete structural and functional domains." Krikos A., Mutoh N., Boyd A., Simon M.I. Cell 33:615-622(1983) [PubMed: 6305515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01705 Genomic DNA. Translation: AAA23567.1. U00096 Genomic DNA. Translation: AAC74955.1. AP009048 Genomic DNA. Translation: BAA15701.1.
PIR	QRECM2. E64951.
RefSeq	AP_002505.1. NP_416399.1.
3D structure databases
SMR	P07018. Positions 290-516.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10955N.
STRING	P07018.
Genome annotation databases
GeneID	946397.
GenomeReviews	Gene locus JW1874 in contig AP009048_GR. Gene locus b1885 in contig U00096_GR.
KEGG	ecj:JW1874. eco:b1885.
Organism-specific databases
EchoBASE	EB0980.
EcoGene	EG10987. tap.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752668.
OMA	MERAKNG.
Enzyme and pathway databases
BioCyc	EcoCyc:TAP-MON. ECOL168927:B1885-MON.
Gene expression databases
Genevestigator	P07018.
Family and domain databases
InterPro	IPR004090. Chemotax_Me-accpt_rcpt. IPR003122. Chemotax_Me-accpt_rcpt_lig-bd. IPR004091. Chemotax_Me-accpt_rcpt_Me-site. IPR004089. Chemotax_Me-accpt_rcpt_sig. IPR003660. HAMP_linker_domain. [Graphical view]
Gene3D	G3DSA:1.20.120.30. Chemotax_Me-accpt_rcpt_lig-bd. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF00015. MCPsignal. 1 hit. PF02203. TarH. 1 hit. [Graphical view]
PRINTS	PR00260. CHEMTRNSDUCR.
SMART	SM00304. HAMP. 1 hit. SM00283. MA. 1 hit. SM00319. TarH. 1 hit. [Graphical view]
PROSITE	PS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit. PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit. PS50885. HAMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCP4_ECOLI

Accession

Primary (citable) accession number: P07018
Secondary accession number(s): P76300

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	November 1, 1997
Last modified:	December 15, 2009
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)