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Protein

Methyl-accepting chemotaxis protein IV

Gene

tap

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates taxis toward dipeptides via an interaction with the periplasmic dipeptide-binding protein.
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.

GO - Molecular functioni

  • signal transducer activity Source: EcoCyc
  • transmembrane signaling receptor activity Source: EcoCyc

GO - Biological processi

  • chemotaxis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

BioCyciEcoCyc:TAP-MONOMER.
ECOL316407:JW1874-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-accepting chemotaxis protein IV
Short name:
MCP-IV
Alternative name(s):
Dipeptide chemoreceptor protein
Gene namesi
Name:tap
Ordered Locus Names:b1885, JW1874
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10987. tap.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

  • Note: Found predominantly at cell poles.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 33HelicalSequence analysisAdd BLAST27
Topological domaini34 – 188PeriplasmicSequence analysisAdd BLAST155
Transmembranei189 – 209HelicalSequence analysisAdd BLAST21
Topological domaini210 – 533CytoplasmicSequence analysisAdd BLAST324

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001105411 – 533Methyl-accepting chemotaxis protein IVAdd BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei293Glutamate methyl ester (Gln)By similarity1
Modified residuei300Glutamate methyl ester (Gln)By similarity1
Modified residuei307Glutamate methyl ester (Gln)By similarity1
Modified residuei489Glutamate methyl ester (Glu)By similarity1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP07018.
PRIDEiP07018.

Interactioni

Protein-protein interaction databases

BioGridi4262072. 163 interactors.
DIPiDIP-10955N.
IntActiP07018. 2 interactors.
MINTiMINT-1309514.
STRINGi511145.b1885.

Structurei

3D structure databases

ProteinModelPortaliP07018.
SMRiP07018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini212 – 264HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini269 – 498Methyl-accepting transducerPROSITE-ProRule annotationAdd BLAST230

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 methyl-accepting transducer domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000148074.
InParanoidiP07018.
KOiK05877.
OMAiYQISTLV.
PhylomeDBiP07018.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00181. Tar_Tsr_LBD. 1 hit.
Gene3Di1.20.120.30. 1 hit.
InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF47170. SSF47170. 1 hit.
PROSITEiPS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07018-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNRIRISTT LFLILILCGI LQIGSNGMSF WAFRDDLQRL NQVEQSNQQR
60 70 80 90 100
AALAQTRAVM LQASTALNKA GTLTALSYPA DDIKTLMTTA RASLTQSTTL
110 120 130 140 150
FKSFMAMTAG NEHVRGLQKE TEKSFARWHN DLEHQATWLE SNQLSDFLTA
160 170 180 190 200
PVQGSQNAFD VNFEAWQLEI NHVLEAASAQ SQRNYQISAL VFISMIIVAA
210 220 230 240 250
IYISSALWWT RKMIVQPLAI IGSHFDSIAA GNLARPIAVY GRNEITAIFA
260 270 280 290 300
SLKTMQQALR GTVSDVRKGS QEMHIGIAEI VAGNNDLSSR TEQQAASLAQ
310 320 330 340 350
TAASMEQLTA TVGQNADNAR QASELAKNAA TTAQAGGVQV STMTHTMQEI
360 370 380 390 400
ATSSQKIGDI ISVIDGIAFQ TNILALNAAV EAARAGEQGR GFAVVAGEVR
410 420 430 440 450
NLASRSAQAA KEIKGLIEES VNRVQQGSKL VNNAAATMID IVSSVTRVND
460 470 480 490 500
IMGEIASASE EQQRGIEQVA QAVSQMDQVT QQNASLVEEA AVATEQLANQ
510 520 530
ADHLSSRVAV FTLEEHEVAR HESVQLQIAP VVS
Length:533
Mass (Da):57,512
Last modified:November 1, 1997 - v2
Checksum:i632570BE1E45DA38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335A → G in AAA23567 (PubMed:6305515).Curated1
Sequence conflicti503H → R in AAA23567 (PubMed:6305515).Curated1
Sequence conflicti527 – 533QIAPVVS → TNCASGILK in AAA23567 (PubMed:6305515).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01705 Genomic DNA. Translation: AAA23567.1.
U00096 Genomic DNA. Translation: AAC74955.1.
AP009048 Genomic DNA. Translation: BAA15701.1.
PIRiE64951. QRECM2.
RefSeqiNP_416399.1. NC_000913.3.
WP_000483239.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74955; AAC74955; b1885.
BAA15701; BAA15701; BAA15701.
GeneIDi946397.
KEGGiecj:JW1874.
eco:b1885.
PATRICi32119097. VBIEscCol129921_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01705 Genomic DNA. Translation: AAA23567.1.
U00096 Genomic DNA. Translation: AAC74955.1.
AP009048 Genomic DNA. Translation: BAA15701.1.
PIRiE64951. QRECM2.
RefSeqiNP_416399.1. NC_000913.3.
WP_000483239.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07018.
SMRiP07018.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262072. 163 interactors.
DIPiDIP-10955N.
IntActiP07018. 2 interactors.
MINTiMINT-1309514.
STRINGi511145.b1885.

Proteomic databases

PaxDbiP07018.
PRIDEiP07018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74955; AAC74955; b1885.
BAA15701; BAA15701; BAA15701.
GeneIDi946397.
KEGGiecj:JW1874.
eco:b1885.
PATRICi32119097. VBIEscCol129921_1966.

Organism-specific databases

EchoBASEiEB0980.
EcoGeneiEG10987. tap.

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000148074.
InParanoidiP07018.
KOiK05877.
OMAiYQISTLV.
PhylomeDBiP07018.

Enzyme and pathway databases

BioCyciEcoCyc:TAP-MONOMER.
ECOL316407:JW1874-MONOMER.

Miscellaneous databases

PROiP07018.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00181. Tar_Tsr_LBD. 1 hit.
Gene3Di1.20.120.30. 1 hit.
InterProiIPR004090. Chemotax_Me-accpt_rcpt.
IPR003122. Chemotax_Me-accpt_rcpt_lig-bd.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
[Graphical view]
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
[Graphical view]
SUPFAMiSSF47170. SSF47170. 1 hit.
PROSITEiPS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCP4_ECOLI
AccessioniPrimary (citable) accession number: P07018
Secondary accession number(s): P76300
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.