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Reviewed, UniProtKB/Swiss-Prot P07014 (DHSB_ECOLI)

Last modified June 16, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Succinate dehydrogenase iron-sulfur subunit
    EC=1.3.99.1
Gene names
Name: sdhB
Ordered Locus Names: b0724, JW0714
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.

Catalytic activity

Succinate + acceptor = fumarate + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster.

Binds 1 3Fe-4S cluster.

Binds 1 4Fe-4S cluster.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle.

Subunit structure

Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein. The complex forms trimers. Ref.7

Subcellular location

Cell inner membrane; Peripheral membrane protein. Ref.7

Sequence similarities

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S ferredoxin-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-1035514,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Succinate dehydrogenase iron-sulfur subunit
PRO_0000158688

Regions

Domain8 – 97902Fe-2S ferredoxin-type
Domain139 – 169314Fe-4S ferredoxin-type

Sites

Metal binding551Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding601Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding631Iron-sulfur 1 (2Fe-2S) Potential
Metal binding751Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1491Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1521Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1551Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1591Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2061Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2121Iron-sulfur 3 (3Fe-4S) By similarity
Metal binding2161Iron-sulfur 2 (4Fe-4S) By similarity

Secondary structure

............................................... 238
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07014-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 226F60C55F5AC35A

FASTA23826,770
        10         20         30         40         50         60 
MRLEFSIYRY NPDVDDAPRM QDYTLEADEG RDMMLLDALI QLKEKDPSLS FRRSCREGVC 

        70         80         90        100        110        120 
GSDGLNMNGK NGLACITPIS ALNQPGKKIV IRPLPGLPVI RDLVVDMGQF YAQYEKIKPY 

       130        140        150        160        170        180 
LLNNGQNPPA REHLQMPEQR EKLDGLYECI LCACCSTSCP SFWWNPDKFI GPAGLLAAYR 

       190        200        210        220        230 
FLIDSRDTET DSRLDGLSDA FSVFRCHSIM NCVSVCPKGL NPTRAIGHIK SMLLQRNA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli."
Darlison M.G., Guest J.R.
Biochem. J. 223:507-517(1984) [PubMed: 6388571] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12."
Darlison M.G., Spencer M.E., Guest J.R.
Eur. J. Biochem. 141:351-359(1984) [PubMed: 6376123] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-238.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-11.
Strain: K12 / EMG2.
[7]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.

Cross-references

Sequence databases

J01619 Genomic DNA. Translation: AAA23896.1.
X01070 Genomic DNA. Translation: CAA25534.1.
U00096 Genomic DNA. Translation: AAC73818.1.
AP009048 Genomic DNA. Translation: BAA35391.1.
X00661 Genomic DNA. Translation: CAA25279.1.
PIRDEECSI. A28837.
RefSeqAP_001362.1.
NP_415252.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60B1-238[»]
1NENX-ray2.90B1-238[»]
2ACZX-ray3.10B1-238[»]
2AD0model-B1-238[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10836N.
IntActP07014. 4 interactions.

Genome annotation databases

GeneID945300.
GenomeReviewsGene locus JW0714 in contig AP009048_GR.
Gene locus b0724 in contig U00096_GR.
KEGGecj:JW0714.
eco:b0724.

Organism-specific databases

EchoBASEEB0925.
EcoGeneEG10932. sdhB.
CMRSearch...

Phylogenomic databases

HOGENOMP07014.
OMAP07014. ADSRDDN.

Enzyme and pathway databases

BioCycEcoCyc:SDH-FE-S.
MetaCyc:SDH-FE-S.

Family and domain databases

InterProIPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. b-grasp_ferredoxin-like.
IPR001041. Ferredoxin.
IPR012285. Fum_reductase_C.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
[Graphical view]
Gene3DG3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMsTIGR00384. dhsB. 1 hit.
PROSITEPS00197. 2FE2S_FER_1. False negative.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHSB_ECOLI
AccessionPrimary (citable) accession number: P07014
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 16, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents