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P07014

- SDHB_ECOLI

UniProt

P07014 - SDHB_ECOLI

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Protein

Succinate dehydrogenase iron-sulfur subunit

Gene

sdhB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Binds 1 2Fe-2S cluster.
Binds 1 3Fe-4S cluster.
Binds 1 4Fe-4S cluster.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (2Fe-2S)
Metal bindingi60 – 601Iron-sulfur 1 (2Fe-2S)
Metal bindingi75 – 751Iron-sulfur 1 (2Fe-2S)
Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S)
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)
Metal bindingi159 – 1591Iron-sulfur 3 (3Fe-4S)
Binding sitei164 – 1641Ubiquinone; shared with SdhD subunit1 Publication
Metal bindingi206 – 2061Iron-sulfur 3 (3Fe-4S)
Metal bindingi212 – 2121Iron-sulfur 3 (3Fe-4S)
Metal bindingi216 – 2161Iron-sulfur 2 (4Fe-4S)

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: EcoCyc
  2. 3 iron, 4 sulfur cluster binding Source: EcoCyc
  3. 4 iron, 4 sulfur cluster binding Source: EcoCyc
  4. electron carrier activity Source: EcoCyc
  5. iron-sulfur cluster binding Source: EcoCyc
  6. metal ion binding Source: UniProtKB-KW
  7. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. aerobic respiration Source: EcoCyc
  2. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:SDH-FE-S.
ECOL316407:JW0714-MONOMER.
MetaCyc:SDH-FE-S.
UniPathwayiUPA00223; UER01005.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase iron-sulfur subunit (EC:1.3.5.1)
Gene namesi
Name:sdhB
Ordered Locus Names:b0724, JW0714
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10932. sdhB.

Subcellular locationi

Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Succinate dehydrogenase iron-sulfur subunitPRO_0000158688Add
BLAST

Proteomic databases

PaxDbiP07014.
PRIDEiP07014.

Expressioni

Gene expression databases

GenevestigatoriP07014.

Interactioni

Subunit structurei

Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein. The complex forms trimers.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
sdhAP0AC412EBI-1035514,EBI-371263

Protein-protein interaction databases

BioGridi849677. 1 interaction.
DIPiDIP-10836N.
IntActiP07014. 8 interactions.
STRINGi511145.b0724.

Structurei

Secondary structure

1
238
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98
Turni12 – 143
Beta strandi19 – 268
Beta strandi29 – 313
Helixi35 – 4511
Beta strandi54 – 607
Beta strandi64 – 674
Beta strandi70 – 734
Helixi74 – 763
Helixi79 – 813
Beta strandi89 – 924
Beta strandi97 – 1015
Beta strandi103 – 1053
Helixi108 – 1169
Beta strandi130 – 1323
Helixi137 – 1415
Turni142 – 1487
Helixi156 – 1583
Helixi160 – 1645
Turni166 – 1683
Helixi172 – 18211
Helixi190 – 1956
Turni200 – 2056
Helixi211 – 2155
Helixi222 – 23716

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60B1-238[»]
1NENX-ray2.90B1-238[»]
2ACZX-ray3.10B1-238[»]
2AD0model-B1-238[»]
2WDQX-ray2.40B/F/J1-238[»]
2WDRX-ray3.20B/F/J1-238[»]
2WDVX-ray3.20B/F/J1-238[»]
2WP9X-ray2.70B/F/J1-238[»]
2WS3X-ray3.20B/F/J1-238[»]
2WU2X-ray2.50B/F/J1-238[»]
2WU5X-ray2.80B/F/J1-238[»]
ProteinModelPortaliP07014.
SMRiP07014. Positions 1-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07014.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 97902Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini139 – 169314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
InParanoidiP07014.
KOiK00240.
OMAiQYEKIRP.
OrthoDBiEOG6CK7MG.
PhylomeDBiP07014.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07014-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLEFSIYRY NPDVDDAPRM QDYTLEADEG RDMMLLDALI QLKEKDPSLS
60 70 80 90 100
FRRSCREGVC GSDGLNMNGK NGLACITPIS ALNQPGKKIV IRPLPGLPVI
110 120 130 140 150
RDLVVDMGQF YAQYEKIKPY LLNNGQNPPA REHLQMPEQR EKLDGLYECI
160 170 180 190 200
LCACCSTSCP SFWWNPDKFI GPAGLLAAYR FLIDSRDTET DSRLDGLSDA
210 220 230
FSVFRCHSIM NCVSVCPKGL NPTRAIGHIK SMLLQRNA
Length:238
Mass (Da):26,770
Last modified:April 1, 1988 - v1
Checksum:i226F60C55F5AC35A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01619 Genomic DNA. Translation: AAA23896.1.
X01070 Genomic DNA. Translation: CAA25534.1.
U00096 Genomic DNA. Translation: AAC73818.1.
AP009048 Genomic DNA. Translation: BAA35391.1.
X00661 Genomic DNA. Translation: CAA25279.1.
PIRiA28837. DEECSI.
RefSeqiNP_415252.1. NC_000913.3.
YP_489004.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73818; AAC73818; b0724.
BAA35391; BAA35391; BAA35391.
GeneIDi12932956.
945300.
KEGGiecj:Y75_p0704.
eco:b0724.
PATRICi32116643. VBIEscCol129921_0754.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01619 Genomic DNA. Translation: AAA23896.1 .
X01070 Genomic DNA. Translation: CAA25534.1 .
U00096 Genomic DNA. Translation: AAC73818.1 .
AP009048 Genomic DNA. Translation: BAA35391.1 .
X00661 Genomic DNA. Translation: CAA25279.1 .
PIRi A28837. DEECSI.
RefSeqi NP_415252.1. NC_000913.3.
YP_489004.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1NEK X-ray 2.60 B 1-238 [» ]
1NEN X-ray 2.90 B 1-238 [» ]
2ACZ X-ray 3.10 B 1-238 [» ]
2AD0 model - B 1-238 [» ]
2WDQ X-ray 2.40 B/F/J 1-238 [» ]
2WDR X-ray 3.20 B/F/J 1-238 [» ]
2WDV X-ray 3.20 B/F/J 1-238 [» ]
2WP9 X-ray 2.70 B/F/J 1-238 [» ]
2WS3 X-ray 3.20 B/F/J 1-238 [» ]
2WU2 X-ray 2.50 B/F/J 1-238 [» ]
2WU5 X-ray 2.80 B/F/J 1-238 [» ]
ProteinModelPortali P07014.
SMRi P07014. Positions 1-238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 849677. 1 interaction.
DIPi DIP-10836N.
IntActi P07014. 8 interactions.
STRINGi 511145.b0724.

Proteomic databases

PaxDbi P07014.
PRIDEi P07014.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73818 ; AAC73818 ; b0724 .
BAA35391 ; BAA35391 ; BAA35391 .
GeneIDi 12932956.
945300.
KEGGi ecj:Y75_p0704.
eco:b0724.
PATRICi 32116643. VBIEscCol129921_0754.

Organism-specific databases

EchoBASEi EB0925.
EcoGenei EG10932. sdhB.

Phylogenomic databases

eggNOGi COG0479.
HOGENOMi HOG000160590.
InParanoidi P07014.
KOi K00240.
OMAi QYEKIRP.
OrthoDBi EOG6CK7MG.
PhylomeDBi P07014.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01005 .
BioCyci EcoCyc:SDH-FE-S.
ECOL316407:JW0714-MONOMER.
MetaCyc:SDH-FE-S.

Miscellaneous databases

EvolutionaryTracei P07014.
PROi P07014.

Gene expression databases

Genevestigatori P07014.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view ]
Pfami PF13085. Fer2_3. 1 hit.
[Graphical view ]
SUPFAMi SSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR00384. dhsB. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli."
    Darlison M.G., Guest J.R.
    Biochem. J. 223:507-517(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12."
    Darlison M.G., Spencer M.E., Guest J.R.
    Eur. J. Biochem. 141:351-359(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-238.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-11.
    Strain: K12 / EMG2.
  7. Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BL21-DE3.
  8. "Architecture of succinate dehydrogenase and reactive oxygen species generation."
    Yankovskaya V., Horsefield R., Toernroth S., Luna-Chavez C., Miyoshi H., Leger C., Byrne B., Cecchini G., Iwata S.
    Science 299:700-704(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS AND UBIQUINONE, COFACTOR, SUBUNIT.
  9. "Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction."
    Horsefield R., Yankovskaya V., Sexton G., Whittingham W., Shiomi K., Omura S., Byrne B., Cecchini G., Iwata S.
    J. Biol. Chem. 281:7309-7316(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS, COFACTOR, SUBUNIT.
  10. "Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site."
    Ruprecht J., Yankovskaya V., Maklashina E., Iwata S., Cecchini G.
    J. Biol. Chem. 284:29836-29846(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiSDHB_ECOLI
AccessioniPrimary (citable) accession number: P07014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: October 29, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3