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P07014 (SDHB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Succinate dehydrogenase iron-sulfur subunit

EC=1.3.5.1
Gene names
Name:sdhB
Ordered Locus Names:b0724, JW0714
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth.

Catalytic activity

Succinate + a quinone = fumarate + a quinol.

Cofactor

Binds 1 2Fe-2S cluster. Ref.8 Ref.9 Ref.10

Binds 1 3Fe-4S cluster. Ref.8 Ref.9 Ref.10

Binds 1 4Fe-4S cluster. Ref.8 Ref.9 Ref.10

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (bacterial route): step 1/1.

Subunit structure

Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and a hydrophobic anchor protein. The complex forms trimers. Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Cell inner membrane; Peripheral membrane protein Ref.7.

Sequence similarities

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S ferredoxin-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

sdhAP0AC416EBI-1035514,EBI-371263

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Succinate dehydrogenase iron-sulfur subunit
PRO_0000158688

Regions

Domain8 – 97902Fe-2S ferredoxin-type
Domain139 – 169314Fe-4S ferredoxin-type

Sites

Metal binding551Iron-sulfur 1 (2Fe-2S)
Metal binding601Iron-sulfur 1 (2Fe-2S)
Metal binding751Iron-sulfur 1 (2Fe-2S)
Metal binding1491Iron-sulfur 2 (4Fe-4S)
Metal binding1521Iron-sulfur 2 (4Fe-4S)
Metal binding1551Iron-sulfur 2 (4Fe-4S)
Metal binding1591Iron-sulfur 3 (3Fe-4S)
Metal binding2061Iron-sulfur 3 (3Fe-4S)
Metal binding2121Iron-sulfur 3 (3Fe-4S)
Metal binding2161Iron-sulfur 2 (4Fe-4S)
Binding site1641Ubiquinone; shared with SdhD subunit

Secondary structure

................................................. 238
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P07014 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 226F60C55F5AC35A

FASTA23826,770
        10         20         30         40         50         60 
MRLEFSIYRY NPDVDDAPRM QDYTLEADEG RDMMLLDALI QLKEKDPSLS FRRSCREGVC 

        70         80         90        100        110        120 
GSDGLNMNGK NGLACITPIS ALNQPGKKIV IRPLPGLPVI RDLVVDMGQF YAQYEKIKPY 

       130        140        150        160        170        180 
LLNNGQNPPA REHLQMPEQR EKLDGLYECI LCACCSTSCP SFWWNPDKFI GPAGLLAAYR 

       190        200        210        220        230 
FLIDSRDTET DSRLDGLSDA FSVFRCHSIM NCVSVCPKGL NPTRAIGHIK SMLLQRNA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli."
Darlison M.G., Guest J.R.
Biochem. J. 223:507-517(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12."
Darlison M.G., Spencer M.E., Guest J.R.
Eur. J. Biochem. 141:351-359(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-238.
[6]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-11.
Strain: K12 / EMG2.
[7]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.
[8]"Architecture of succinate dehydrogenase and reactive oxygen species generation."
Yankovskaya V., Horsefield R., Toernroth S., Luna-Chavez C., Miyoshi H., Leger C., Byrne B., Cecchini G., Iwata S.
Science 299:700-704(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS AND UBIQUINONE, COFACTOR, SUBUNIT.
[9]"Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction."
Horsefield R., Yankovskaya V., Sexton G., Whittingham W., Shiomi K., Omura S., Byrne B., Cecchini G., Iwata S.
J. Biol. Chem. 281:7309-7316(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS, COFACTOR, SUBUNIT.
[10]"Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site."
Ruprecht J., Yankovskaya V., Maklashina E., Iwata S., Cecchini G.
J. Biol. Chem. 284:29836-29846(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR CLUSTERS, COFACTOR, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01619 Genomic DNA. Translation: AAA23896.1.
X01070 Genomic DNA. Translation: CAA25534.1.
U00096 Genomic DNA. Translation: AAC73818.1.
AP009048 Genomic DNA. Translation: BAA35391.1.
X00661 Genomic DNA. Translation: CAA25279.1.
PIRDEECSI. A28837.
RefSeqNP_415252.1. NC_000913.3.
YP_489004.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60B1-238[»]
1NENX-ray2.90B1-238[»]
2ACZX-ray3.10B1-238[»]
2AD0model-B1-238[»]
2WDQX-ray2.40B/F/J1-238[»]
2WDRX-ray3.20B/F/J1-238[»]
2WDVX-ray3.20B/F/J1-238[»]
2WP9X-ray2.70B/F/J1-238[»]
2WS3X-ray3.20B/F/J1-238[»]
2WU2X-ray2.50B/F/J1-238[»]
2WU5X-ray2.80B/F/J1-238[»]
ProteinModelPortalP07014.
SMRP07014. Positions 1-238.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid849677. 1 interaction.
DIPDIP-10836N.
IntActP07014. 6 interactions.
STRING511145.b0724.

Proteomic databases

PaxDbP07014.
PRIDEP07014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73818; AAC73818; b0724.
BAA35391; BAA35391; BAA35391.
GeneID12932956.
945300.
KEGGecj:Y75_p0704.
eco:b0724.
PATRIC32116643. VBIEscCol129921_0754.

Organism-specific databases

EchoBASEEB0925.
EcoGeneEG10932. sdhB.

Phylogenomic databases

eggNOGCOG0479.
HOGENOMHOG000160590.
KOK00240.
OMAEGVWRCR.
OrthoDBEOG6CK7MG.
PhylomeDBP07014.
ProtClustDBPRK05950.

Enzyme and pathway databases

BioCycEcoCyc:SDH-FE-S.
ECOL316407:JW0714-MONOMER.
MetaCyc:SDH-FE-S.
UniPathwayUPA00223; UER01005.

Gene expression databases

GenevestigatorP07014.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR00384. dhsB. 1 hit.
PROSITEPS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP07014.
PROP07014.

Entry information

Entry nameSDHB_ECOLI
AccessionPrimary (citable) accession number: P07014
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: April 16, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene