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Protein

Primosomal replication protein N

Gene

priB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.UniRule annotation3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • single-stranded DNA binding Source: EcoCyc

GO - Biological processi

  • DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  • DNA replication initiation Source: EcoCyc
  • plasmid maintenance Source: EcoCyc
  • response to radiation Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG10764-MONOMER.
MetaCyc:EG10764-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Primosomal replication protein NUniRule annotation
Gene namesi
Name:priBUniRule annotation
Ordered Locus Names:b4201, JW4159
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10764. priB.

Subcellular locationi

GO - Cellular componenti

  • pre-primosome complex Source: UniProtKB

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001990502 – 104Primosomal replication protein NAdd BLAST103

Proteomic databases

PaxDbiP07013.
PRIDEiP07013.

Expressioni

Inductioni

Induced by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Component of the preprimosomal complex composed of one monomer of PriC and DnaT, two monomers of PriA, two dimers of PriB and one hexamer of DnaB. Upon transient interaction with DnaG it forms the primosome.UniRule annotation1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-10563N.
IntActiP07013. 12 interactors.
STRINGi316407.85676952.

Structurei

Secondary structure

1104
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 20Combined sources18
Turni21 – 23Combined sources3
Beta strandi24 – 39Combined sources16
Beta strandi42 – 57Combined sources16
Helixi60 – 64Combined sources5
Beta strandi71 – 76Combined sources6
Beta strandi84 – 86Combined sources3
Beta strandi91 – 93Combined sources3
Beta strandi95 – 99Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TXYX-ray2.00A/B1-104[»]
1V1QX-ray2.10A/B1-102[»]
1WOCX-ray2.00A/B/C/D2-104[»]
2CCZX-ray2.70A/B1-102[»]
2PNHX-ray2.25A/B1-104[»]
ProteinModelPortaliP07013.
SMRiP07013.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07013.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 101SSBUniRule annotationAdd BLAST100

Sequence similaritiesi

Belongs to the PriB family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4108VDV. Bacteria.
COG2965. LUCA.
HOGENOMiHOG000261221.
KOiK02686.

Family and domain databases

CDDicd04496. SSB_OBF. 1 hit.
HAMAPiMF_00720. PriB. 1 hit.
InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR023646. Prisomal_replication_PriB.
PfamiView protein in Pfam
PF00436. SSB. 1 hit.
PIRSFiPIRSF003135. Primosomal_n. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR04418. PriB_gamma. 1 hit.
PROSITEiView protein in PROSITE
PS50935. SSB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRLVLSGT VCRAPLRKVS PSGIPHCQFV LEHRSVQEEA GFHRQAWCQM
60 70 80 90 100
PVIVSGHENQ AITHSITVGS RITVQGFISC HKAKNGLSKM VLHAEQIELI

DSGD
Length:104
Mass (Da):11,442
Last modified:January 23, 2007 - v3
Checksum:i0D52F9D68193B4AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04022 Genomic DNA. Translation: CAA27653.1.
U14003 Genomic DNA. Translation: AAA97097.1.
U00096 Genomic DNA. Translation: AAC77158.1.
AP009048 Genomic DNA. Translation: BAE78202.1.
PIRiA30281. Q4ECFR.
RefSeqiNP_418622.1. NC_000913.3.
WP_001315977.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77158; AAC77158; b4201.
BAE78202; BAE78202; BAE78202.
GeneIDi948722.
KEGGiecj:JW4159.
eco:b4201.
PATRICifig|1411691.4.peg.2500.

Similar proteinsi

Entry informationi

Entry nameiPRIB_ECOLI
AccessioniPrimary (citable) accession number: P07013
Secondary accession number(s): Q2M6A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families