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Protein

Peptide chain release factor 2

Gene

prfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.

GO - Molecular functioni

  • ribosome binding Source: GO_Central
  • translation release factor activity, codon specific Source: EcoCyc

GO - Biological processi

  • translational termination Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10762-MONOMER.
ECOL316407:JW5847-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 2
Short name:
RF-2
Gene namesi
Name:prfB
Synonyms:supK
Ordered Locus Names:b2891, JW5847
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10762. prfB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi252 – 2521Q → E: Loss of methylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Peptide chain release factor 2PRO_0000166816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei252 – 2521N5-methylglutamine2 Publications

Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF2. Is absent when the factor is overproduced.3 Publications

Keywords - PTMi

Methylation

Proteomic databases

EPDiP07012.
PaxDbiP07012.
PRIDEiP07012.

Interactioni

Protein-protein interaction databases

DIPiDIP-10559N.
IntActiP07012. 19 interactions.
MINTiMINT-1238444.
STRINGi511145.b2891.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2419Combined sources
Helixi27 – 4216Combined sources
Helixi44 – 485Combined sources
Helixi50 – 9041Combined sources
Helixi93 – 11321Combined sources
Helixi114 – 1185Combined sources
Turni122 – 1254Combined sources
Beta strandi128 – 1347Combined sources
Helixi138 – 15821Combined sources
Beta strandi162 – 1709Combined sources
Beta strandi172 – 18514Combined sources
Helixi188 – 1925Combined sources
Helixi193 – 1953Combined sources
Beta strandi197 – 2048Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi213 – 22412Combined sources
Helixi236 – 2383Combined sources
Beta strandi239 – 2446Combined sources
Beta strandi260 – 2656Combined sources
Turni266 – 2683Combined sources
Beta strandi271 – 2744Combined sources
Beta strandi276 – 2783Combined sources
Helixi280 – 30627Combined sources
Beta strandi321 – 3277Combined sources
Helixi328 – 3303Combined sources
Beta strandi332 – 3354Combined sources
Turni336 – 3383Combined sources
Beta strandi341 – 3433Combined sources
Helixi345 – 3495Combined sources
Helixi354 – 3629Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GQEX-ray1.81A1-365[»]
1MI6electron microscopy10.90A1-365[»]
1ML5electron microscopy14.00Z1-365[»]
ProteinModelPortaliP07012.
SMRiP07012. Positions 4-365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07012.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CG1. Bacteria.
COG1186. LUCA.
HOGENOMiHOG000074814.
InParanoidiP07012.
KOiK02836.
OMAiRLYEHEM.
PhylomeDBiP07012.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00094. Rel_fac_2. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR020853. Peptide_chain_release_fac2_bac.
IPR004374. PrfB.
[Graphical view]
PANTHERiPTHR11075:SF6. PTHR11075:SF6. 1 hit.
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00020. prfB. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEINPVNNR IQDLTERSDV LRGYLDYDAK KERLEEVNAE LEQPDVWNEP
60 70 80 90 100
ERAQALGKER SSLEAVVDTL DQMKQGLEDV SGLLELAVEA DDEETFNEAV
110 120 130 140 150
AELDALEEKL AQLEFRRMFS GEYDSADCYL DIQAGSGGTE AQDWASMLER
160 170 180 190 200
MYLRWAESRG FKTEIIEESE GEVAGIKSVT IKISGDYAYG WLRTETGVHR
210 220 230 240 250
LVRKSPFDSG GRRHTSFSSA FVYPEVDDDI DIEINPADLR IDVYRTSGAG
260 270 280 290 300
GQHVNRTESA VRITHIPTGI VTQCQNDRSQ HKNKDQAMKQ MKAKLYELEM
310 320 330 340 350
QKKNAEKQAM EDNKSDIGWG SQIRSYVLDD SRIKDLRTGV ETRNTQAVLD
360
GSLDQFIEAS LKAGL
Length:365
Mass (Da):41,251
Last modified:November 1, 1997 - v3
Checksum:iEB8C802FDDE0A76D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011L → V (PubMed:3889910).Curated
Sequence conflicti201 – 2011L → V (PubMed:3049538).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti246 – 2461T → A in strain: BL21 and MRE-600; increased termination efficiency.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11520 Genomic DNA. Translation: AAA24520.1.
U28375 Genomic DNA. Translation: AAA83072.1.
U00096 Genomic DNA. Translation: AAC75929.1.
AP009048 Genomic DNA. Translation: BAE76956.1.
J03795 Genomic DNA. Translation: AAA23958.1.
PIRiC65073. FCECR2.
RefSeqiNP_417367.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75929; AAC75929; b2891.
BAE76956; BAE76956; BAE76956.
GeneIDi947369.
KEGGiecj:JW5847.
eco:b2891.
PATRICi32121192. VBIEscCol129921_2984.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11520 Genomic DNA. Translation: AAA24520.1.
U28375 Genomic DNA. Translation: AAA83072.1.
U00096 Genomic DNA. Translation: AAC75929.1.
AP009048 Genomic DNA. Translation: BAE76956.1.
J03795 Genomic DNA. Translation: AAA23958.1.
PIRiC65073. FCECR2.
RefSeqiNP_417367.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GQEX-ray1.81A1-365[»]
1MI6electron microscopy10.90A1-365[»]
1ML5electron microscopy14.00Z1-365[»]
ProteinModelPortaliP07012.
SMRiP07012. Positions 4-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10559N.
IntActiP07012. 19 interactions.
MINTiMINT-1238444.
STRINGi511145.b2891.

Proteomic databases

EPDiP07012.
PaxDbiP07012.
PRIDEiP07012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75929; AAC75929; b2891.
BAE76956; BAE76956; BAE76956.
GeneIDi947369.
KEGGiecj:JW5847.
eco:b2891.
PATRICi32121192. VBIEscCol129921_2984.

Organism-specific databases

EchoBASEiEB0755.
EcoGeneiEG10762. prfB.

Phylogenomic databases

eggNOGiENOG4105CG1. Bacteria.
COG1186. LUCA.
HOGENOMiHOG000074814.
InParanoidiP07012.
KOiK02836.
OMAiRLYEHEM.
PhylomeDBiP07012.

Enzyme and pathway databases

BioCyciEcoCyc:EG10762-MONOMER.
ECOL316407:JW5847-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP07012.
PROiP07012.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00094. Rel_fac_2. 1 hit.
InterProiIPR014720. dsRBD_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR020853. Peptide_chain_release_fac2_bac.
IPR004374. PrfB.
[Graphical view]
PANTHERiPTHR11075:SF6. PTHR11075:SF6. 1 hit.
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00020. prfB. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRF2_ECOLI
AccessioniPrimary (citable) accession number: P07012
Secondary accession number(s): P76642, Q2M9V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The gene for this protein contains a UGA in-frame termination codon after Leu-25; a naturally occurring frameshift enables complete translation of RF-2. This provides a mechanism for the protein to regulate its own production.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.