P07004 (PROA_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 118.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Gamma-glutamyl phosphate reductase Short name=GPR EC=1.2.1.41 Alternative name(s): Glutamate-5-semialdehyde dehydrogenase Glutamyl-gamma-semialdehyde dehydrogenase Short name=GSA dehydrogenase | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 417 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412 |
| Catalytic activity | L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412 |
| Pathway | Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412 |
| Subcellular location | |
| Sequence similarities | Belongs to the gamma-glutamyl phosphate reductase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Proline biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | proline biosynthetic process Inferred from genetic interaction Ref.1. Source: EcoCyc |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NADP binding Inferred from direct assay. Source: EcoCyc glutamate-5-semialdehyde dehydrogenase activityInferred from direct assay. Source: EcoCyc |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of the Escherichia coli proBA locus by DNA and protein sequencing." Deutch A.H., Rushlow K.E., Smith C.J. Nucleic Acids Res. 12:6337-6355(1984) [PubMed: 6089111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. |
| [2] | "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [3] | "Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 358-367. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "Structure and biosynthesis of unbranched multicopy single-stranded DNA by reverse transcriptase in a clinical Escherichia coli isolate." Lim D. Mol. Microbiol. 6:3531-3542(1992) [PubMed: 1282191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 368-417. Strain: Clinical strain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X00786 Genomic DNA. Translation: CAA25364.1. U70214 Genomic DNA. Translation: AAB08663.1. U00096 Genomic DNA. Translation: AAC73347.1. AP009048 Genomic DNA. Translation: BAA77912.2. Z12832 Genomic DNA. Translation: CAA78292.1. |
| PIR | RDECER. D64749. |
| RefSeq | NP_414778.1. NC_000913.2. |
3D structure databases | |
| ProteinModelPortal | P07004. |
| SMR | P07004. Positions 2-417. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-10568N. |
| IntAct | P07004. 17 interactions. |
| MINT | MINT-1261639. |
2D gel databases | |
| SWISS-2DPAGE | P07004. |
| 2DBase-Ecoli | P07004. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000001314; EBESCP00000001314; EBESCG00000001089. EBESCT00000016949; EBESCP00000016240; EBESCG00000016008. |
| GeneID | 946680. |
| GenomeReviews | Gene locus JW0233 in contig AP009048_GR. Gene locus b0243 in contig U00096_GR. |
| KEGG | ecj:JW0233. eco:b0243. |
| PATRIC | 32115601. VBIEscCol129921_0245. |
Organism-specific databases | |
| EchoBASE | EB0760. |
| EcoGene | EG10767. proA. |
Phylogenomic databases | |
| eggNOG | COG0014. |
| GeneTree | EBGT00050000009178. |
| HOGENOM | HBG318080. |
| OMA | QYPAACN. |
| PhylomeDB | P07004. |
| ProtClustDB | PRK00197. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:GLUTSEMIALDEHYDROG-MONOMER. MetaCyc:GLUTSEMIALDEHYDROG-MONOMER. |
Gene expression databases | |
| Genevestigator | P07004. |
Family and domain databases | |
| HAMAP | MF_00412. ProA. [Tree] |
| InterPro | IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits. |
| KO | K00147. |
| PANTHER | PTHR11063:SF1. GSA_DH. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| PIRSF | PIRSF000151. GPR. 1 hit. |
| SUPFAM | SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit. |
| TIGRFAMs | TIGR00407. ProA. 1 hit. |
| PROSITE | PS01223. PROA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PROA_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P07004 Secondary accession number(s): P77428 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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