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Protein

Gamma-glutamyl phosphate reductase

Gene

proA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.UniRule annotation2 Publications

Catalytic activityi

L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.UniRule annotation2 Publications

Enzyme regulationi

Inhibited by p-chloromercuribenzoate, copper and nickel ions.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Pathwayi: L-proline biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • glutamate-5-semialdehyde dehydrogenase activity Source: EcoCyc
  • NADP binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:GLUTSEMIALDEHYDROG-MONOMER.
ECOL316407:JW0233-MONOMER.
MetaCyc:GLUTSEMIALDEHYDROG-MONOMER.
UniPathwayiUPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl phosphate reductaseUniRule annotation (EC:1.2.1.41UniRule annotation)
Short name:
GPRUniRule annotation
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenaseUniRule annotation
Glutamyl-gamma-semialdehyde dehydrogenaseUniRule annotation
Short name:
GSA dehydrogenaseUniRule annotation
Gene namesi
Name:proAUniRule annotation
Ordered Locus Names:b0243, JW0233
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10767. proA.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Gamma-glutamyl phosphate reductasePRO_0000189723Add
BLAST

Proteomic databases

EPDiP07004.
PaxDbiP07004.
PRIDEiP07004.

2D gel databases

SWISS-2DPAGEP07004.

Interactioni

Subunit structurei

Homodimer (Probable). May form a complex with glutamate 5-kinase (proB).Curated2 Publications

Protein-protein interaction databases

BioGridi4259768. 16 interactions.
DIPiDIP-10568N.
IntActiP07004. 17 interactions.
MINTiMINT-1261639.
STRINGi511145.b0243.

Structurei

3D structure databases

ProteinModelPortaliP07004.
SMRiP07004. Positions 16-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the gamma-glutamyl phosphate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C2S. Bacteria.
COG0014. LUCA.
HOGENOMiHOG000246356.
InParanoidiP07004.
KOiK00147.
OMAiCNAIETL.
PhylomeDBiP07004.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEQMGIAAK QASYKLAQLS SREKNRVLEK IADELEAQSE IILNANAQDV
60 70 80 90 100
ADARANGLSE AMLDRLALTP ARLKGIADDV RQVCNLADPV GQVIDGGVLD
110 120 130 140 150
SGLRLERRRV PLGVIGVIYE ARPNVTVDVA SLCLKTGNAV ILRGGKETCR
160 170 180 190 200
TNAATVAVIQ DALKSCGLPA GAVQAIDNPD RALVSEMLRM DKYIDMLIPR
210 220 230 240 250
GGAGLHKLCR EQSTIPVITG GIGVCHIYVD ESVEIAEALK VIVNAKTQRP
260 270 280 290 300
STCNTVETLL VNKNIADSFL PALSKQMAES GVTLHADAAA LAQLQAGPAK
310 320 330 340 350
VVAVKAEEYD DEFLSLDLNV KIVSDLDDAI AHIREHGTQH SDAILTRDMR
360 370 380 390 400
NAQRFVNEVD SSAVYVNAST RFTDGGQFGL GAEVAVSTQK LHARGPMGLE
410
ALTTYKWIGI GDYTIRA
Length:417
Mass (Da):44,630
Last modified:November 1, 1997 - v2
Checksum:iD9A4E3418EFEB488
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti358 – 36710Missing (PubMed:6089111).Curated
Sequence conflicti358 – 36710Missing (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00786 Genomic DNA. Translation: CAA25364.1.
U70214 Genomic DNA. Translation: AAB08663.1.
U00096 Genomic DNA. Translation: AAC73347.1.
AP009048 Genomic DNA. Translation: BAA77912.2.
Z12832 Genomic DNA. Translation: CAA78292.1.
PIRiD64749. RDECER.
RefSeqiNP_414778.1. NC_000913.3.
WP_000893278.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73347; AAC73347; b0243.
BAA77912; BAA77912; BAA77912.
GeneIDi946680.
KEGGiecj:JW0233.
eco:b0243.
PATRICi32115601. VBIEscCol129921_0245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00786 Genomic DNA. Translation: CAA25364.1.
U70214 Genomic DNA. Translation: AAB08663.1.
U00096 Genomic DNA. Translation: AAC73347.1.
AP009048 Genomic DNA. Translation: BAA77912.2.
Z12832 Genomic DNA. Translation: CAA78292.1.
PIRiD64749. RDECER.
RefSeqiNP_414778.1. NC_000913.3.
WP_000893278.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP07004.
SMRiP07004. Positions 16-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259768. 16 interactions.
DIPiDIP-10568N.
IntActiP07004. 17 interactions.
MINTiMINT-1261639.
STRINGi511145.b0243.

2D gel databases

SWISS-2DPAGEP07004.

Proteomic databases

EPDiP07004.
PaxDbiP07004.
PRIDEiP07004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73347; AAC73347; b0243.
BAA77912; BAA77912; BAA77912.
GeneIDi946680.
KEGGiecj:JW0233.
eco:b0243.
PATRICi32115601. VBIEscCol129921_0245.

Organism-specific databases

EchoBASEiEB0760.
EcoGeneiEG10767. proA.

Phylogenomic databases

eggNOGiENOG4105C2S. Bacteria.
COG0014. LUCA.
HOGENOMiHOG000246356.
InParanoidiP07004.
KOiK00147.
OMAiCNAIETL.
PhylomeDBiP07004.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00360.
BioCyciEcoCyc:GLUTSEMIALDEHYDROG-MONOMER.
ECOL316407:JW0233-MONOMER.
MetaCyc:GLUTSEMIALDEHYDROG-MONOMER.

Miscellaneous databases

PROiP07004.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR020593. G-glutamylP_reductase_CS.
IPR012134. Glu-5-SA_DH.
IPR000965. GPR_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF000151. GPR. 1 hit.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00407. proA. 1 hit.
PROSITEiPS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROA_ECOLI
AccessioniPrimary (citable) accession number: P07004
Secondary accession number(s): P77428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.