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UniProtKB - P07003 (POXB_ECOLI)

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Protein

Pyruvate dehydrogenase [ubiquinone]

Gene

poxB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Pyruvate + ubiquinone + H2O = acetate + CO2 + ubiquinol.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication
  • Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
  • thiamine diphosphate1 PublicationNote: Binds 1 thiamine pyrophosphate per subunit.1 Publication

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • lipid binding Source: EcoCyc
  • magnesium ion binding Source: EcoCyc
  • pyruvate dehydrogenase (quinone) activity Source: EcoCyc
  • thiamine pyrophosphate binding Source: EcoCyc
Complete GO annotation...

GO - Biological processi

  • protein homotetramerization Source: EcoCyc
  • pyruvate catabolic process Source: EcoCyc
  • pyruvate metabolic process Source: EcoCyc
Complete GO annotation...

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, Lipid-binding, Magnesium, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciEcoCyc:PYRUVOXID-MONOMER.
MetaCyc:PYRUVOXID-MONOMER.
BRENDAi1.2.5.1. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase [ubiquinone] (EC:1.2.5.1)
Alternative name(s):
Pyruvate oxidase
Short name:
POX
Cleaved into the following chain:
Alpha-peptide
Gene namesi
Name:poxB
Ordered Locus Names:b0871, JW0855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10754. poxB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi533A → T in poxB11. 1 Publication1
Mutagenesisi549 – 572Missing in poxB6. 1 PublicationAdd BLAST24
Mutagenesisi553A → V in poxB14. 1 Publication1
Mutagenesisi560D → P in poxB15; normal activity. 1 Publication1
Mutagenesisi564 – 572Missing in poxB7. 1 Publication9
Mutagenesisi564E → P in poxB16; loss of activity. 1 Publication1
Mutagenesisi570 – 572Missing in poxB8. 1 Publication3
Mutagenesisi572R → G in poxB10; reduced activity; may interact less with membranes. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3380.
DrugBankiDB00336. Nitrofural.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000446061 – 572Pyruvate dehydrogenase [ubiquinone]Add BLAST572
PeptideiPRO_0000035665550 – 572Alpha-peptideAdd BLAST23

Post-translational modificationi

Activated by limited proteolytic digestion. This cleavage produces a peptide (alpha-peptide) and mimics the activation of enzyme by phospholipids. The proteolytic cleavage also results in the loss of the high affinity lipid-binding site of the enzyme.

Proteomic databases

EPDiP07003.
PaxDbiP07003.
PRIDEiP07003.

2D gel databases

SWISS-2DPAGEiP07003.

Interactioni

Subunit structurei

Homotetramer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
Complete GO annotation...

Protein-protein interaction databases

BioGridi4262101. 12 interactors.
DIPiDIP-36216N.
IntActiP07003. 4 interactors.
STRINGi316385.ECDH10B_0941.

Structurei

Secondary structure

1572
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 15Combined sources11
Beta strandi20 – 23Combined sources4
Helixi27 – 29Combined sources3
Helixi30 – 39Combined sources10
Beta strandi41 – 46Combined sources6
Helixi50 – 64Combined sources15
Beta strandi68 – 72Combined sources5
Helixi76 – 79Combined sources4
Helixi82 – 90Combined sources9
Beta strandi95 – 102Combined sources8
Helixi104 – 106Combined sources3
Helixi117 – 120Combined sources4
Turni121 – 124Combined sources4
Beta strandi126 – 130Combined sources5
Helixi134 – 136Combined sources3
Helixi137 – 150Combined sources14
Beta strandi153 – 160Combined sources8
Helixi161 – 164Combined sources4
Beta strandi183 – 185Combined sources3
Helixi188 – 199Combined sources12
Beta strandi203 – 208Combined sources6
Helixi210 – 212Combined sources3
Helixi216 – 226Combined sources11
Beta strandi230 – 232Combined sources3
Helixi234 – 236Combined sources3
Helixi237 – 240Combined sources4
Beta strandi241 – 243Combined sources3
Beta strandi247 – 250Combined sources4
Helixi257 – 265Combined sources9
Beta strandi267 – 273Combined sources7
Helixi279 – 281Combined sources3
Beta strandi284 – 293Combined sources10
Helixi295 – 297Combined sources3
Beta strandi300 – 302Combined sources3
Beta strandi305 – 309Combined sources5
Helixi312 – 319Combined sources8
Helixi320 – 322Combined sources3
Helixi330 – 348Combined sources19
Beta strandi349 – 351Combined sources3
Beta strandi354 – 357Combined sources4
Helixi359 – 369Combined sources11
Beta strandi375 – 378Combined sources4
Helixi382 – 390Combined sources9
Beta strandi398 – 400Combined sources3
Turni403 – 405Combined sources3
Helixi411 – 421Combined sources11
Beta strandi427 – 432Combined sources6
Helixi433 – 438Combined sources6
Helixi440 – 442Combined sources3
Helixi443 – 448Combined sources6
Beta strandi454 – 459Combined sources6
Beta strandi461 – 466Combined sources6
Beta strandi468 – 470Combined sources3
Turni478 – 480Combined sources3
Helixi487 – 493Combined sources7
Beta strandi496 – 501Combined sources6
Helixi504 – 506Combined sources3
Helixi507 – 516Combined sources10
Beta strandi517 – 519Combined sources3
Beta strandi521 – 527Combined sources7
Helixi536 – 542Combined sources7
Turni543 – 545Combined sources3
Helixi548 – 550Combined sources3
Beta strandi551 – 555Combined sources5
Beta strandi559 – 564Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EY9X-ray2.90A/B1-572[»]
3EYAX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-549[»]
ProteinModelPortaliP07003.
SMRiP07003.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07003.

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105C7K. Bacteria.
COG0028. LUCA.
InParanoidiP07003.
KOiK00156.
PhylomeDBiP07003.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
PfamiView protein in Pfam
PF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
PROSITEiView protein in PROSITE
PS00187. TPP_ENZYMES. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P07003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQTVAAYIA KTLESAGVKR IWGVTGDSLN GLSDSLNRMG TIEWMSTRHE 
        60         70         80         90        100
EVAAFAAGAE AQLSGELAVC AGSCGPGNLH LINGLFDCHR NHVPVLAIAA 
       110        120        130        140        150
HIPSSEIGSG YFQETHPQEL FRECSHYCEL VSSPEQIPQV LAIAMRKAVL 
       160        170        180        190        200
NRGVSVVVLP GDVALKPAPE GATMHWYHAP QPVVTPEEEE LRKLAQLLRY 
       210        220        230        240        250
SSNIALMCGS GCAGAHKELV EFAGKIKAPI VHALRGKEHV EYDNPYDVGM 
       260        270        280        290        300
TGLIGFSSGF HTMMNADTLV LLGTQFPYRA FYPTDAKIIQ IDINPASIGA 
       310        320        330        340        350
HSKVDMALVG DIKSTLRALL PLVEEKADRK FLDKALEDYR DARKGLDDLA 
       360        370        380        390        400
KPSEKAIHPQ YLAQQISHFA ADDAIFTCDV GTPTVWAARY LKMNGKRRLL 
       410        420        430        440        450
GSFNHGSMAN AMPQALGAQA TEPERQVVAM CGDGGFSMLM GDFLSVVQMK 
       460        470        480        490        500
LPVKIVVFNN SVLGFVAMEM KAGGYLTDGT ELHDTNFARI AEACGITGIR 
       510        520        530        540        550
VEKASEVDEA LQRAFSIDGP VLVDVVVAKE ELAIPPQIKL EQAKGFSLYM 
       560        570 
LRAIISGRGD EVIELAKTNW LR
Length:572
Mass (Da):62,011
Last modified:April 1, 1988 - v1
Checksum:i57B38B9E3A92BDEA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti364 – 365QQ → HE in AAB59101 (PubMed:2663858).Curated2
Sequence conflicti364 – 365QQ → HE in AAB59102 (PubMed:2663858).Curated2
Sequence conflicti414 – 416QAL → HGV in AAB59101 (PubMed:2663858).Curated3
Sequence conflicti414 – 416QAL → HGV in AAB59102 (PubMed:2663858).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04105 Genomic DNA. Translation: CAA27725.1.
U00096 Genomic DNA. Translation: AAC73958.1.
AP009048 Genomic DNA. Translation: BAA35585.1.
S73268 Genomic DNA. Translation: AAB31180.1.
M28208 Genomic DNA. Translation: AAB59101.1.
L47688 Genomic DNA. Translation: AAB59102.1.
L47689 Genomic DNA. Translation: AAB59103.1.
L47690 Genomic DNA. Translation: AAB59104.1.
L47691 Genomic DNA. Translation: AAB59105.1.
L47692 Genomic DNA. Translation: AAB59106.1.
L47693 Genomic DNA. Translation: AAB59107.1.
L47694 Genomic DNA. Translation: AAB59108.1.
L47695 Genomic DNA. Translation: AAB59109.1.
PIRiA23648. DEECPC.
RefSeqiNP_415392.1. NC_000913.3.
WP_000815337.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73958; AAC73958; b0871.
BAA35585; BAA35585; BAA35585.
GeneIDi946132.
KEGGiecj:JW0855.
eco:b0871.
PATRICifig|1411691.4.peg.1406.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPOXB_ECOLI
AccessioniPrimary (citable) accession number: P07003
Secondary accession number(s): Q47513
, Q47514, Q47515, Q47516, Q47517, Q47518, Q47519, Q47520
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: July 5, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families