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Protein

NAD(P) transhydrogenase subunit alpha

Gene

pntA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Catalytic activityi

NADPH + NAD+ = NADP+ + NADH.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175NAD; via amide nitrogen1 Publication1
Binding sitei238NAD1 Publication1
Binding sitei257NAD; via carbonyl oxygen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi120 – 122NAD1 Publication3
Nucleotide bindingi195 – 197NAD1 Publication3

GO - Molecular functioni

  • NAD(P)+ transhydrogenase (AB-specific) activity Source: UniProtKB
  • NAD binding Source: EcoCyc
  • NADP binding Source: GO_Central
  • protein dimerization activity Source: UniProtKB

GO - Biological processi

  • NADPH regeneration Source: UniProtKB
  • proton transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PNTA-MONOMER.
ECOL316407:JW1595-MONOMER.
MetaCyc:PNTA-MONOMER.
BRENDAi1.6.1.2. 2026.

Protein family/group databases

TCDBi3.D.2.1.1. the proton-translocating transhydrogenase (pth) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P) transhydrogenase subunit alpha (EC:1.6.1.2)
Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit alpha
Pyridine nucleotide transhydrogenase subunit alpha
Gene namesi
Name:pntA
Ordered Locus Names:b1603, JW1595
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10744. pntA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 401CytoplasmicSequence analysisAdd BLAST401
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 452CytoplasmicSequence analysis9
Transmembranei453 – 473HelicalSequence analysisAdd BLAST21
Topological domaini474 – 476PeriplasmicSequence analysis3
Transmembranei477 – 497HelicalSequence analysisAdd BLAST21
Topological domaini498 – 510CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151 – 161Missing : Dimerizes; affinity for PntB unchanged, but maximum transhydrogenation rate lowered significantly. Add BLAST11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001990171 – 510NAD(P) transhydrogenase subunit alphaAdd BLAST510

Proteomic databases

EPDiP07001.
PaxDbiP07001.
PRIDEiP07001.

Interactioni

Subunit structurei

Heterodimer of an alpha (PntA) and a beta (PntB) chain. Alpha subunit serves as the dimerization unit.2 Publications

GO - Molecular functioni

  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi4260811. 6 interactors.
DIPiDIP-366N.
IntActiP07001. 2 interactors.
MINTiMINT-1299705.
STRINGi511145.b1603.

Structurei

Secondary structure

1510
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi20 – 28Combined sources9
Beta strandi32 – 36Combined sources5
Turni37 – 40Combined sources4
Helixi41 – 43Combined sources3
Helixi47 – 53Combined sources7
Beta strandi56 – 58Combined sources3
Helixi60 – 64Combined sources5
Beta strandi65 – 70Combined sources6
Helixi77 – 80Combined sources4
Beta strandi88 – 91Combined sources4
Helixi95 – 97Combined sources3
Helixi99 – 107Combined sources9
Beta strandi111 – 114Combined sources4
Helixi115 – 117Combined sources3
Helixi122 – 127Combined sources6
Helixi129 – 148Combined sources20
Beta strandi156 – 158Combined sources3
Beta strandi161 – 163Combined sources3
Beta strandi167 – 171Combined sources5
Helixi175 – 186Combined sources12
Beta strandi190 – 194Combined sources5
Helixi198 – 200Combined sources3
Helixi201 – 206Combined sources6
Beta strandi209 – 211Combined sources3
Helixi225 – 230Combined sources6
Helixi232 – 248Combined sources17
Beta strandi250 – 254Combined sources5
Beta strandi259 – 261Combined sources3
Helixi269 – 273Combined sources5
Beta strandi280 – 283Combined sources4
Helixi286 – 288Combined sources3
Beta strandi291 – 294Combined sources4
Beta strandi299 – 302Combined sources4
Turni304 – 306Combined sources3
Beta strandi308 – 310Combined sources3
Helixi316 – 318Combined sources3
Helixi320 – 338Combined sources19
Beta strandi341 – 344Combined sources4
Beta strandi350 – 352Combined sources3
Helixi353 – 358Combined sources6
Beta strandi359 – 362Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X13X-ray1.90A/B2-394[»]
1X14X-ray1.94A/B2-394[»]
1X15X-ray2.04A/B2-394[»]
2BRUNMR-A/B2-394[»]
ProteinModelPortaliP07001.
SMRiP07001.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP07001.

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108IIE. Bacteria.
COG3288. LUCA.
HOGENOMiHOG000022121.
InParanoidiP07001.
KOiK00324.
OMAiNVAPKEF.
PhylomeDBiP07001.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR008143. Ala_DH/PNT_CS2.
IPR008142. AlaDH/PNT_CS1.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR023753. FAD/NAD-binding_dom.
IPR016040. NAD(P)-bd_dom.
IPR026255. NADP_transhyd_a.
IPR024605. NADP_transhyd_a_C.
[Graphical view]
PfamiPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF12769. PNTB_4TM. 1 hit.
[Graphical view]
PIRSFiPIRSF000203. NADP_transhydrogenase_alpha. 1 hit.
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00561. pntA. 1 hit.
PROSITEiPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P07001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGIPRERL TNETRVAATP KTVEQLLKLG FTVAVESGAG QLASFDDKAF
60 70 80 90 100
VQAGAEIVEG NSVWQSEIIL KVNAPLDDEI ALLNPGTTLV SFIWPAQNPE
110 120 130 140 150
LMQKLAERNV TVMAMDSVPR ISRAQSLDAL SSMANIAGYR AIVEAAHEFG
160 170 180 190 200
RFFTGQITAA GKVPPAKVMV IGAGVAGLAA IGAANSLGAI VRAFDTRPEV
210 220 230 240 250
KEQVQSMGAE FLELDFKEEA GSGDGYAKVM SDAFIKAEME LFAAQAKEVD
260 270 280 290 300
IIVTTALIPG KPAPKLITRE MVDSMKAGSV IVDLAAQNGG NCEYTVPGEI
310 320 330 340 350
FTTENGVKVI GYTDLPGRLP TQSSQLYGTN LVNLLKLLCK EKDGNITVDF
360 370 380 390 400
DDVVIRGVTV IRAGEITWPA PPIQVSAQPQ AAQKAAPEVK TEEKCTCSPW
410 420 430 440 450
RKYALMALAI ILFGWMASVA PKEFLGHFTV FALACVVGYY VVWNVSHALH
460 470 480 490 500
TPLMSVTNAI SGIIVVGALL QIGQGGWVSF LSFIAVLIAS INIFGGFTVT
510
QRMLKMFRKN
Length:510
Mass (Da):54,623
Last modified:April 1, 1993 - v2
Checksum:i801742097BEA6943
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04195 Genomic DNA. Translation: CAB37089.1.
X66086 Genomic DNA. Translation: CAA46884.1.
U00096 Genomic DNA. Translation: AAC74675.1.
AP009048 Genomic DNA. Translation: BAA15342.1.
PIRiS24380. DEECXA.
RefSeqiNP_416120.1. NC_000913.3.
WP_001300486.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74675; AAC74675; b1603.
BAA15342; BAA15342; BAA15342.
GeneIDi946628.
KEGGiecj:JW1595.
eco:b1603.
PATRICi32118508. VBIEscCol129921_1674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04195 Genomic DNA. Translation: CAB37089.1.
X66086 Genomic DNA. Translation: CAA46884.1.
U00096 Genomic DNA. Translation: AAC74675.1.
AP009048 Genomic DNA. Translation: BAA15342.1.
PIRiS24380. DEECXA.
RefSeqiNP_416120.1. NC_000913.3.
WP_001300486.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X13X-ray1.90A/B2-394[»]
1X14X-ray1.94A/B2-394[»]
1X15X-ray2.04A/B2-394[»]
2BRUNMR-A/B2-394[»]
ProteinModelPortaliP07001.
SMRiP07001.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260811. 6 interactors.
DIPiDIP-366N.
IntActiP07001. 2 interactors.
MINTiMINT-1299705.
STRINGi511145.b1603.

Protein family/group databases

TCDBi3.D.2.1.1. the proton-translocating transhydrogenase (pth) family.

Proteomic databases

EPDiP07001.
PaxDbiP07001.
PRIDEiP07001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74675; AAC74675; b1603.
BAA15342; BAA15342; BAA15342.
GeneIDi946628.
KEGGiecj:JW1595.
eco:b1603.
PATRICi32118508. VBIEscCol129921_1674.

Organism-specific databases

EchoBASEiEB0737.
EcoGeneiEG10744. pntA.

Phylogenomic databases

eggNOGiENOG4108IIE. Bacteria.
COG3288. LUCA.
HOGENOMiHOG000022121.
InParanoidiP07001.
KOiK00324.
OMAiNVAPKEF.
PhylomeDBiP07001.

Enzyme and pathway databases

BioCyciEcoCyc:PNTA-MONOMER.
ECOL316407:JW1595-MONOMER.
MetaCyc:PNTA-MONOMER.
BRENDAi1.6.1.2. 2026.

Miscellaneous databases

EvolutionaryTraceiP07001.
PROiP07001.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR008143. Ala_DH/PNT_CS2.
IPR008142. AlaDH/PNT_CS1.
IPR007886. AlaDH/PNT_N.
IPR007698. AlaDH/PNT_NAD(H)-bd.
IPR023753. FAD/NAD-binding_dom.
IPR016040. NAD(P)-bd_dom.
IPR026255. NADP_transhyd_a.
IPR024605. NADP_transhyd_a_C.
[Graphical view]
PfamiPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
PF12769. PNTB_4TM. 1 hit.
[Graphical view]
PIRSFiPIRSF000203. NADP_transhydrogenase_alpha. 1 hit.
SMARTiSM01002. AlaDh_PNT_C. 1 hit.
SM01003. AlaDh_PNT_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00561. pntA. 1 hit.
PROSITEiPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNTA_ECOLI
AccessioniPrimary (citable) accession number: P07001
Secondary accession number(s): P76888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.